RchiOBHm_Chr2g0100361
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003077
Physical Location & Seq
Forward (+)
12328379 .. 12334373
5995 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47500

Sequence Viewer

Length: 531 bp
ATGTGCATCATCTCTGCCTTGAATCTCTTGAAGAAACAATCGATCTTCTCTCCTACTTTTTGCTTGGTGTTGAATAAGGTCGCCGGCCATGTCCTTTTTTTGCTTGCGACTGCAGAAATATTGAGAGAAGAAGTTGGCTGCAAGTGGGTTTTCAGGGTGAAACGCAATCCGGATGGCTCTGTTGAACGCTACAAGGCTCGTTTTGTGGCTAAAGGCTTTCACCAACAACACGATATTGACTATAATGAGACGTTTAGTTCTGTGATTAAGCCAGCCACAATTCGTAATGTACTCTGTCTTACAATTTCTCGCAGTTGGCCTCTTCGTCAGTTAGACGTTAAGAATGTCTTCCTCCATGTTTTTCTTAAGGAAGACGTCTACATGACCCAGCCTCCTAGCTTCATTGATCAAGCAAGACCGTCGTTTGTCTGCAAGCTCAACAAAGCTATCTATGGCCTTAAACAAGCCCCTCATGCCTGGTTCCATCGCATGACTTCTTTTCTATTGTCTGTTGGTTTTGTGCAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

176

Amino Acids

20.25

Weight (kDa)

9.71

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 43 - 175 2.6e-38 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 378
AccI GTMKAC 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 169
AcoI YGGCCR 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 1 cut(s) 291
AflII CTTAAG 1 cut(s) 365
AgsI TTSAA 4 cut(s) 22, 31, 73, 185
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 3 cut(s) 399, 436, 446
AluI AGCT 3 cut(s) 399, 436, 446
Alw26I GTCTC 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 3 cut(s) 85, 317, 454
ApeKI GCWGC 1 cut(s) 138
Asp700I GAANNNNTTC 1 cut(s) 347
AsuHPI GGTGA 2 cut(s) 169, 212
BbsI GAAGAC 2 cut(s) 340, 378
BbvI GCAGC 1 cut(s) 125
BccI CCATC 2 cut(s) 167, 492
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 1 cut(s) 111
BfrI CTTAAG 1 cut(s) 365
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme1390I CCNGG 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 482
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 340, 378
Bsa29I ATCGAT 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 375
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bse118I RCCGGY 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 1 cut(s) 478
BseCI ATCGAT 1 cut(s) 41
BseGI GGATG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 125
BseYI CCCAGC 1 cut(s) 387
BshFI GGCC 3 cut(s) 87, 319, 456
BshVI ATCGAT 1 cut(s) 41
BsiSI CCGG 2 cut(s) 84, 170
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsnI GGCC 3 cut(s) 87, 319, 456
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 2 cut(s) 42, 406
BspANI GGCC 3 cut(s) 87, 319, 456
BspDI ATCGAT 1 cut(s) 41
BspEI TCCGGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 482
BspMAI CTGCAG 1 cut(s) 115
BspTI CTTAAG 1 cut(s) 365
BsrFI RCCGGY 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 83
BssMI GATC 2 cut(s) 42, 406
BssNI GRCGYC 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 420
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 1 cut(s) 375
BstAFI CTTAAG 1 cut(s) 365
BstC8I GCNNGC 4 cut(s) 85, 105, 273, 434
BstF5I GGATG 1 cut(s) 178
BstKTI GATC 2 cut(s) 45, 409
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 2 cut(s) 42, 406
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 1 cut(s) 478
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 125
BstV2I GAAGAC 2 cut(s) 340, 378
Bsu15I ATCGAT 1 cut(s) 41
BsuRI GGCC 3 cut(s) 87, 319, 456
BsuTUI ATCGAT 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 470
BtsCI GGATG 1 cut(s) 178
Cac8I GCNNGC 4 cut(s) 85, 105, 273, 434
Cfr10I RCCGGY 1 cut(s) 83
ClaI ATCGAT 1 cut(s) 41
Csp6I GTAC 1 cut(s) 290
CviAII CATG 5 cut(s) 89, 356, 382, 473, 490
CviQI GTAC 1 cut(s) 290
DpnI GATC 2 cut(s) 44, 408
DpnII GATC 2 cut(s) 42, 406
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EcoRII CCWGG 1 cut(s) 476
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 5 cut(s) 92, 359, 385, 476, 493
FaiI YATR 7 cut(s) 90, 243, 357, 383, 453, 474, 491
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FatI CATG 5 cut(s) 88, 355, 381, 472, 489
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 139
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 1 cut(s) 396
GluI GCNGC 1 cut(s) 139
GsaI CCCAGC 1 cut(s) 391
HaeIII GGCC 3 cut(s) 87, 319, 456
HapII CCGG 2 cut(s) 84, 170
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 5 cut(s) 92, 359, 385, 476, 493
HinfI GANTC 1 cut(s) 22
HpaII CCGG 2 cut(s) 84, 170
HphI GGTGA 2 cut(s) 169, 212
Hpy166II GTNNAC 1 cut(s) 379
Hpy188III TCNNGA 2 cut(s) 28, 170
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 420
HpyCH4IV ACGT 3 cut(s) 251, 336, 375
HpyCH4V TGCA 5 cut(s) 6, 113, 141, 432, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpySE526I ACGT 3 cut(s) 251, 336, 375
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 5 cut(s) 92, 359, 385, 476, 493
Kpn2I TCCGGA 1 cut(s) 169
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 2 cut(s) 42, 406
LpnPI CCDG 7 cut(s) 97, 139, 183, 285, 401, 463, 490
Lsp1109I GCAGC 1 cut(s) 125
LweI GCATC 1 cut(s) 15
MaeI CTAG 1 cut(s) 396
MaeII ACGT 3 cut(s) 251, 336, 375
MalI GATC 2 cut(s) 44, 408
MboI GATC 2 cut(s) 42, 406
MboII GAAGA 6 cut(s) 37, 43, 140, 314, 340, 383
MluCI AATT 2 cut(s) 279, 303
MnlI CCTC 4 cut(s) 330, 362, 402, 480
MroI TCCGGA 1 cut(s) 169
MroNI GCCGGC 1 cut(s) 83
MroXI GAANNNNTTC 1 cut(s) 347
MseI TTAA 4 cut(s) 267, 339, 366, 459
MspCI CTTAAG 1 cut(s) 365
MspI CCGG 2 cut(s) 84, 170
MspR9I CCNGG 1 cut(s) 478
MvaI CCWGG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 473
NaeI GCCGGC 1 cut(s) 85
NdeII GATC 2 cut(s) 42, 406
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 5 cut(s) 92, 359, 385, 476, 493
NlaIV GGNNCC 1 cut(s) 482
PdiI GCCGGC 1 cut(s) 85
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 1 cut(s) 482
PstI CTGCAG 1 cut(s) 115
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
SaqAI TTAA 4 cut(s) 267, 339, 366, 459
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 2 cut(s) 42, 406
ScrFI CCNGG 1 cut(s) 478
SetI ASST 7 cut(s) 81, 254, 339, 378, 401, 438, 448
SfaNI GCATC 1 cut(s) 15
SfcI CTRYAG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 2 cut(s) 279, 303
SspI AATATT 1 cut(s) 120
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 420
TaiI ACGT 3 cut(s) 254, 339, 378
TaqI TCGA 1 cut(s) 41
TasI AATT 2 cut(s) 279, 303
TatI WGTACW 1 cut(s) 289
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 4 cut(s) 267, 339, 366, 459
Tru9I TTAA 4 cut(s) 267, 339, 366, 459
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 1 cut(s) 391
Vha464I CTTAAG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 378
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 1 cut(s) 396
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.