RchiOBHm_Chr6g0276521

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003077
Physical Location & Seq
Forward (+)
38581102 .. 38582477
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24807

Sequence Viewer

Length: 1239 bp
ATGACCCAGCCCCCTGGCTTCGTCGATTCCTCTCGGCCCTCCTATGTCTGTAAGCTTCACAAGGCACTTTATGGCCTCAAACAAGCACCCCGTGATTGGTTTCAACGCATGAGTTCTTTTCTGCTTTCTGTTGGTTTTCACCATAGTAAAGCTGATTCATCCTTATTCATCTATCAACATGGCCTGCATGTGATTTTCTTCTTACTCTATGTTGATGATATTGTTGTCACGGGCAGCAGTGACCGGCTTCTTCAAAGTTTTATCGATGCCTTGGGCCGTGGATTTGACATTAAGGATCTTGGTCCCCTACACTATTTCCTTGGTTTACAGGTCACACCTCATTCCAATGGCCTCCTCATCAATCAACTCAAGTATGCTCATGACATTTTAAAGAAGCATGATCTGTTACACAATCAACCAGTGAGCACCCCAATGTCAGCTAAGACTGTTCTCACCACACATGATGGGGACCTTCTTGCAAATCCCACTGTTTTTCGTGAGATGGTTGGCTCACTGCAGTACCTCACTATTACACGGCCTGACATTGCCTTTGCTGTCAATTCTGTGTCCCAATTTATGAGTCATCCTCGTGCTCCCCATCTCATTGCTGCCAAACGTATTCTCCGCTATTTCAAAGGCACTCTGGGCCATGGTCTCTCTTTCAGTCCTCAGTGCCATCCTGTCCACCTATCTGCTTACTCTGATGCCGATTGGGCCGGTTGTCCTGACTCTCGCCGTTCCACTTCCGGCTATCTCATTTACCTTGGCAATACACTTATCTCTTGGTGTTCCAAAAAGCAGCCTACCATTGCTCGCTCCAGTGCAGAATCTGAATATCGATCCCTTGCTCATGCTAGTGCTGAGACAACTTGGTTAGGCTTCTTACTGTATGAGCTTGGTGTCCATATTCAGTACCCCATTCTGCTTTACTGTGATAATCTCAGTGCCACATACATGGCTTCTAATCCGGTCTTCCATGCTCGCACCAAGCACATAGAGTTAGATTATCACTTTCTTCGGGAAAAGGTAGCCCTTGGGAGTCATCAGGTTCATTTTATCCCTTCCATAGATCAACCTGCTGACCTTCTTACTAAACCACTCCACAAGCAGCGCCATACTCTCCTGTGTACCAAACTTGTGCGTCCCGAGCCGCCCAGTTTGAAGGGAGGTGTTAGCGACATTAACTCCTTGCCTCAATCAAGCGTGATCTCTTCCCCAAATCAAGCTCATGAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs

Protein Analysis

412

Amino Acids

46.14

Weight (kDa)

8.87

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 1 - 146 1.6e-33 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1084
AciI CCGC 2 cut(s) 625, 1151
AclWI GGATC 2 cut(s) 303, 834
AdeI CACNNNGTG 1 cut(s) 92
AfaI GTAC 3 cut(s) 521, 914, 1129
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 4 cut(s) 104, 254, 634, 1162
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 5 cut(s) 55, 152, 440, 895, 1226
AluI AGCT 5 cut(s) 55, 152, 440, 895, 1226
Alw21I GWGCWC 2 cut(s) 428, 595
Alw26I GTCTC 2 cut(s) 659, 857
AlwI GGATC 2 cut(s) 303, 834
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 1145
AoxI GGCC 8 cut(s) 35, 73, 181, 274, 349, 536, 646, 714
ApeKI GCWGC 4 cut(s) 234, 608, 799, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AspLEI GCGC 1 cut(s) 1113
AspS9I GGNCC 6 cut(s) 36, 274, 302, 469, 646, 714
AsuHPI GGTGA 2 cut(s) 131, 445
AvaI CYCGRG 1 cut(s) 1145
AvaII GGWCC 2 cut(s) 302, 469
BaeI ACNNNNGTAYC 2 cut(s) 503, 536
BauI CACGAG 1 cut(s) 588
BbsI GAAGAC 1 cut(s) 964
Bbv12I GWGCWC 2 cut(s) 428, 595
BbvI GCAGC 4 cut(s) 246, 595, 811, 1120
BccI CCATC 4 cut(s) 458, 496, 606, 684
BceAI ACGGC 3 cut(s) 261, 551, 720
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 659, 857
BfaI CTAG 1 cut(s) 855
BfmI CTRYAG 1 cut(s) 515
BfoI RGCGCY 1 cut(s) 1114
BfuAI ACCTGC 1 cut(s) 1084
BglI GCCNNNNNGGC 1 cut(s) 713
BisI GCNGC 5 cut(s) 235, 609, 800, 1109, 1151
BlsI GCNGC 5 cut(s) 236, 610, 801, 1110, 1152
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 302, 469
BmeT110I CYCGRG 1 cut(s) 1145
BmgT120I GGNCC 6 cut(s) 36, 274, 302, 469, 646, 714
BmiI GGNNCC 2 cut(s) 304, 470
BmrFI CCNGG 1 cut(s) 15
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 2 cut(s) 256, 694
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 1 cut(s) 964
BplI GAGNNNNNCTC 2 cut(s) 571, 603
BpmI CTGGAG 1 cut(s) 802
BpuEI CTTGAG 1 cut(s) 353
Bsa29I ATCGAT 2 cut(s) 264, 838
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 7 cut(s) 13, 270, 277, 319, 649, 763, 1033
BsaWI WCCGGW 1 cut(s) 967
BsaXI ACNNNNNCTCC 4 cut(s) 606, 636, 1169, 1199
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse118I RCCGGY 2 cut(s) 243, 716
Bse1I ACTGG 3 cut(s) 419, 819, 1155
Bse3DI GCAATG 3 cut(s) 543, 603, 807
BseBI CCWGG 1 cut(s) 15
BseCI ATCGAT 2 cut(s) 264, 838
BseDI CCNNGG 7 cut(s) 13, 270, 277, 319, 649, 763, 1033
BseGI GGATG 3 cut(s) 158, 583, 676
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMI GCAATG 3 cut(s) 543, 603, 807
BseMII CTCAG 3 cut(s) 683, 852, 955
BseNI ACTGG 3 cut(s) 419, 819, 1155
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 4 cut(s) 246, 595, 811, 1120
BseYI CCCAGC 1 cut(s) 6
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 8 cut(s) 37, 75, 183, 276, 351, 538, 648, 716
BshVI ATCGAT 2 cut(s) 264, 838
BsiHKAI GWGCWC 2 cut(s) 428, 595
BsiHKCI CYCGRG 1 cut(s) 1145
BsiSI CCGG 4 cut(s) 244, 717, 747, 968
BslFI GGGAC 4 cut(s) 288, 482, 553, 1128
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 2 cut(s) 659, 857
BsmFI GGGAC 4 cut(s) 288, 482, 553, 1128
BsnI GGCC 8 cut(s) 37, 75, 183, 276, 351, 538, 648, 716
Bso31I GGTCTC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 1145
Bsp1286I GDGCHC 2 cut(s) 428, 595
Bsp143I GATC 5 cut(s) 295, 400, 839, 1069, 1206
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 2 cut(s) 625, 1151
BspANI GGCC 8 cut(s) 37, 75, 183, 276, 351, 538, 648, 716
BspCNI CTCAG 3 cut(s) 682, 853, 954
BspDI ATCGAT 2 cut(s) 264, 838
BspHI TCATGA 2 cut(s) 379, 1228
BspLI GGNNCC 2 cut(s) 304, 470
BspMAI CTGCAG 1 cut(s) 519
BspMI ACCTGC 1 cut(s) 1084
BspPI GGATC 2 cut(s) 303, 834
BspTNI GGTCTC 1 cut(s) 659
BsrDI GCAATG 3 cut(s) 543, 603, 807
BsrFI RCCGGY 2 cut(s) 243, 716
BsrI ACTGG 3 cut(s) 419, 819, 1155
BssAI RCCGGY 2 cut(s) 243, 716
BssECI CCNNGG 7 cut(s) 13, 270, 277, 319, 649, 763, 1033
BssMI GATC 5 cut(s) 295, 400, 839, 1069, 1206
BssSI CACGAG 1 cut(s) 588
BssT1I CCWWGG 5 cut(s) 270, 319, 649, 763, 1033
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 4 cut(s) 448, 490, 888, 932
Bst6I CTCTTC 1 cut(s) 1216
BstC8I GCNNGC 3 cut(s) 185, 814, 982
BstDEI CTNAG 4 cut(s) 441, 669, 861, 941
BstDSI CCRYGG 2 cut(s) 277, 649
BstF5I GGATG 3 cut(s) 158, 583, 676
BstH2I RGCGCY 1 cut(s) 1114
BstHHI GCGC 1 cut(s) 1113
BstKTI GATC 5 cut(s) 298, 403, 842, 1072, 1209
BstMAI GTCTC 2 cut(s) 659, 857
BstMBI GATC 5 cut(s) 295, 400, 839, 1069, 1206
BstMWI GCNNNNNNNGC 3 cut(s) 645, 713, 1147
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 515
BstV1I GCAGC 4 cut(s) 246, 595, 811, 1120
BstV2I GAAGAC 1 cut(s) 964
BstX2I RGATCY 1 cut(s) 295
BstXI CCANNNNNNTGG 2 cut(s) 14, 955
BstYI RGATCY 1 cut(s) 295
Bsu15I ATCGAT 2 cut(s) 264, 838
BsuRI GGCC 8 cut(s) 37, 75, 183, 276, 351, 538, 648, 716
BsuTUI ATCGAT 2 cut(s) 264, 838
BtgI CCRYGG 2 cut(s) 277, 649
BtsCI GGATG 3 cut(s) 158, 583, 676
BtsI GCAGTG 2 cut(s) 244, 512
BtsIMutI CAGTG 7 cut(s) 244, 426, 486, 512, 677, 826, 949
BveI ACCTGC 1 cut(s) 1084
Cac8I GCNNGC 3 cut(s) 185, 814, 982
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 2 cut(s) 379, 1228
CfoI GCGC 1 cut(s) 1113
Cfr10I RCCGGY 2 cut(s) 243, 716
Cfr13I GGNCC 6 cut(s) 36, 274, 302, 469, 646, 714
ClaI ATCGAT 2 cut(s) 264, 838
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 3 cut(s) 520, 913, 1128
CviQI GTAC 3 cut(s) 520, 913, 1128
DdeI CTNAG 4 cut(s) 441, 669, 861, 941
DpnI GATC 5 cut(s) 297, 402, 841, 1071, 1208
DpnII GATC 5 cut(s) 295, 400, 839, 1069, 1206
DraI TTTAAA 1 cut(s) 390
DraIII CACNNNGTG 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 1216
EarI CTCTTC 1 cut(s) 1216
Eco130I CCWWGG 5 cut(s) 270, 319, 649, 763, 1033
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 2 cut(s) 302, 469
Eco88I CYCGRG 1 cut(s) 1145
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 5 cut(s) 270, 319, 649, 763, 1033
ErhI CCWWGG 5 cut(s) 270, 319, 649, 763, 1033
FaqI GGGAC 4 cut(s) 288, 482, 553, 1128
Fnu4HI GCNGC 5 cut(s) 235, 609, 800, 1109, 1151
FokI GGATG 3 cut(s) 145, 570, 663
Fsp4HI GCNGC 5 cut(s) 235, 609, 800, 1109, 1151
FspBI CTAG 1 cut(s) 855
GlaI GCGC 1 cut(s) 1112
GluI GCNGC 5 cut(s) 235, 609, 800, 1109, 1151
GsaI CCCAGC 1 cut(s) 10
GsuI CTGGAG 1 cut(s) 802
HaeII RGCGCY 1 cut(s) 1114
HaeIII GGCC 8 cut(s) 37, 75, 183, 276, 351, 538, 648, 716
HapII CCGG 4 cut(s) 244, 717, 747, 968
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 1 cut(s) 1113
Hin6I GCGC 1 cut(s) 1111
HinP1I GCGC 1 cut(s) 1111
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 6 cut(s) 26, 155, 580, 728, 827, 1039
HpaII CCGG 4 cut(s) 244, 717, 747, 968
HphI GGTGA 2 cut(s) 131, 445
Hpy166II GTNNAC 3 cut(s) 326, 685, 1128
Hpy188I TCNGA 2 cut(s) 703, 832
Hpy188III TCNNGA 6 cut(s) 380, 497, 725, 1019, 1145, 1229
Hpy8I GTNNAC 3 cut(s) 326, 685, 1128
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 4 cut(s) 482, 1071, 1094, 1156
HpyCH4III ACNGT 4 cut(s) 448, 490, 888, 932
HpyCH4IV ACGT 1 cut(s) 616
HpyCH4V TGCA 4 cut(s) 187, 479, 517, 824
HpyF10VI GCNNNNNNNGC 3 cut(s) 645, 713, 1147
HpyF3I CTNAG 4 cut(s) 441, 669, 861, 941
HpySE526I ACGT 1 cut(s) 616
HspAI GCGC 1 cut(s) 1111
Kzo9I GATC 5 cut(s) 295, 400, 839, 1069, 1206
LmnI GCTCC 2 cut(s) 598, 821
Lsp1109I GCAGC 4 cut(s) 246, 595, 811, 1120
LweI GCATC 2 cut(s) 256, 694
MaeI CTAG 1 cut(s) 855
MaeII ACGT 1 cut(s) 616
MaeIII GTNAC 4 cut(s) 226, 239, 331, 405
MalI GATC 5 cut(s) 297, 402, 841, 1071, 1208
MboI GATC 5 cut(s) 295, 400, 839, 1069, 1206
MboII GAAGA 5 cut(s) 190, 242, 964, 1007, 1203
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 2 cut(s) 428, 595
MluCI AATT 2 cut(s) 559, 572
MlyI GAGTC 3 cut(s) 589, 722, 1048
MseI TTAA 3 cut(s) 291, 389, 1182
MslI CAYNNNNRTG 5 cut(s) 345, 431, 588, 855, 953
MspI CCGG 4 cut(s) 244, 717, 747, 968
MspR9I CCNGG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 645, 713, 1147
NcoI CCATGG 1 cut(s) 649
NdeII GATC 5 cut(s) 295, 400, 839, 1069, 1206
NlaIV GGNNCC 2 cut(s) 304, 470
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 3 cut(s) 226, 239, 331
NspI RCATGY 1 cut(s) 191
PagI TCATGA 2 cut(s) 379, 1228
PfeI GAWTC 3 cut(s) 26, 155, 827
PkrI GCNGC 5 cut(s) 236, 610, 801, 1110, 1152
PleI GAGTC 3 cut(s) 588, 722, 1047
PpsI GAGTC 3 cut(s) 588, 722, 1047
PpuMI RGGWCCY 1 cut(s) 469
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 6
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 304, 470
PspPI GGNCC 6 cut(s) 36, 274, 302, 469, 646, 714
PspPPI RGGWCCY 1 cut(s) 469
PstI CTGCAG 1 cut(s) 519
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 3 cut(s) 521, 914, 1129
RsaNI GTAC 3 cut(s) 520, 913, 1128
RseI CAYNNNNRTG 5 cut(s) 345, 431, 588, 855, 953
SaqAI TTAA 3 cut(s) 291, 389, 1182
SatI GCNGC 5 cut(s) 235, 609, 800, 1109, 1151
Sau3AI GATC 5 cut(s) 295, 400, 839, 1069, 1206
Sau96I GGNCC 6 cut(s) 36, 274, 302, 469, 646, 714
SchI GAGTC 3 cut(s) 589, 722, 1048
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 2 cut(s) 428, 595
SfaNI GCATC 2 cut(s) 256, 694
SfcI CTRYAG 1 cut(s) 515
SinI GGWCC 2 cut(s) 302, 469
SmiMI CAYNNNNRTG 5 cut(s) 345, 431, 588, 855, 953
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 559, 572
SsiI CCGC 2 cut(s) 625, 1151
SspMI CTAG 1 cut(s) 855
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 5 cut(s) 270, 319, 649, 763, 1033
TaaI ACNGT 4 cut(s) 448, 490, 888, 932
TaiI ACGT 1 cut(s) 619
TaqI TCGA 3 cut(s) 24, 264, 838
TasI AATT 2 cut(s) 559, 572
TauI GCSGC 1 cut(s) 1153
TfiI GAWTC 3 cut(s) 26, 155, 827
Tru1I TTAA 3 cut(s) 291, 389, 1182
Tru9I TTAA 3 cut(s) 291, 389, 1182
TscAI CASTG 7 cut(s) 244, 426, 493, 519, 677, 826, 949
TseFI GTSAC 3 cut(s) 226, 239, 331
TseI GCWGC 4 cut(s) 234, 608, 799, 1108
Tsp45I GTSAC 3 cut(s) 226, 239, 331
TspDTI ATGAA 3 cut(s) 147, 157, 1040
TspRI CASTG 7 cut(s) 244, 426, 493, 519, 677, 826, 949
VpaK11BI GGWCC 2 cut(s) 302, 469
XceI RCATGY 1 cut(s) 191
XspI CTAG 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.