Rw4G014500

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003077
Physical Location & Seq
Forward (+)
33590027 .. 33591576
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014500.1

Sequence Viewer

Length: 963 bp
ATGACAGAGGAGATAAATGCCTTGTTGAAGAACAACACCTGGACACTGGTGCCTTTCTCCCCAACACAGAATACAGTTGGCTGTAAGTGGGTATTTCGGGTGAAGCGAAGTCTGATGGATCGATCGAGCGCTACAAGGCTCGTCTGGTACAAACCTGTGAGCACACCTATGTCTGCTAAGTCTGACCTCAATACCCCTAATGGTGTAGTCCTTGAGAATCCTACCTCCTTCCGTGAACTTGTTGGGTCCTTGCAATATCTCACCATCACACGACCTGACATTGCCTTTGCAGTTAACACTGTCTCACAGTTTATGAGCCAGCCACATGTGTCTCATCTTGTTGCTGTAAAACGCATCCTCCGTTACATCAAAGGCACTATTGATCATGGTTTACTGTTCACACCACAACCCCAACCGGTCACTCTGTCTACTTACTCTGATGCTGATTGGGCCGGCTGTCCCGTTACTCGTCGCTCCACATCTGGCTATCTTGTGTACCTAGGTTCTAATCTTATCTCTTGGTGTTCTAAAAAGCAACCTACCATTGCTTGCTCTAGTGCAGAGTCCGAGTATCGCTCTCTTGCCCATGCTAGTGCCGAAACAACTTGGTTGGGTTATTTACTGTATGAGCTTGGTGCCTGGACTCAATTTCCCATCTTGTTACATTGTGATAATCTGAGTGCCACTTATATGGCCTCCAATCCTGTTTTTCATGCACGCACTAAGCATATTGAACAGGATTACCACTATGTTCGTGAAAAGGTTGCTTGTGGTAGTCATCGCGTTTGTTTCATTCCATCCAAAGATCAGCTAGCAGATTTGCTCACTAAGCCACTTCACAAATCTCGCCATCGTCTCTTTTCCGGCAAACTTGTCCGTCCAGGACCGCCAAGTTTGAGGGGGGCTGTTAGAGACAATCAATCTCCTCCACCAATCAATCCCAGAATCTCCTCCATTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs

Protein Analysis

320

Amino Acids

35.9

Weight (kDa)

9.51

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 49, 635
AccI GTMKAC 1 cut(s) 428
AccII CGCG 1 cut(s) 783
AciI CCGC 1 cut(s) 887
AclWI GGATC 1 cut(s) 126
AfaI GTAC 2 cut(s) 149, 497
AfeI AGCGCT 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 325
AgeI ACCGGT 1 cut(s) 415
AgsI TTSAA 3 cut(s) 28, 734, 959
AjnI CCWGG 3 cut(s) 38, 638, 880
AluBI AGCT 2 cut(s) 631, 811
AluI AGCT 2 cut(s) 631, 811
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 4 cut(s) 307, 336, 860, 906
AlwI GGATC 1 cut(s) 126
Aor51HI AGCGCT 1 cut(s) 130
AoxI GGCC 2 cut(s) 450, 693
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AsiGI ACCGGT 1 cut(s) 415
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 246, 450, 884
AsuHPI GGTGA 2 cut(s) 112, 253
AsuNHI GCTAGC 1 cut(s) 811
AvaII GGWCC 2 cut(s) 246, 884
AvrII CCTAGG 1 cut(s) 499
BanI GGYRCC 2 cut(s) 49, 635
Bbv12I GWGCWC 1 cut(s) 164
BccI CCATC 5 cut(s) 109, 272, 662, 805, 858
BciT130I CCWGG 3 cut(s) 40, 640, 882
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 4 cut(s) 307, 336, 860, 906
BfaI CTAG 4 cut(s) 500, 555, 591, 812
BfoI RGCGCY 1 cut(s) 132
BlnI CCTAGG 1 cut(s) 499
Bme1390I CCNGG 3 cut(s) 40, 640, 882
Bme18I GGWCC 2 cut(s) 246, 884
BmgT120I GGNCC 3 cut(s) 246, 450, 884
BmiI GGNNCC 3 cut(s) 51, 247, 637
BmrFI CCNGG 3 cut(s) 40, 640, 882
BmsI GCATC 2 cut(s) 363, 430
BmtI GCTAGC 1 cut(s) 815
BplI GAGNNNNNCTC 2 cut(s) 560, 592
BpuEI CTTGAG 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 499
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bse118I RCCGGY 2 cut(s) 415, 452
Bse1I ACTGG 1 cut(s) 51
Bse3DI GCAATG 2 cut(s) 279, 543
BseBI CCWGG 3 cut(s) 40, 640, 882
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 499
BseGI GGATG 2 cut(s) 354, 797
BseMI GCAATG 2 cut(s) 279, 543
BseMII CTCAG 1 cut(s) 668
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 3 cut(s) 23, 915, 940
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 783
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 2 cut(s) 452, 695
BshNI GGYRCC 2 cut(s) 49, 635
BshTI ACCGGT 1 cut(s) 415
BshVI ATCGAT 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 3 cut(s) 416, 453, 864
BslFI GGGAC 1 cut(s) 444
BsmAI GTCTC 4 cut(s) 307, 336, 860, 906
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 444
BsnI GGCC 2 cut(s) 452, 695
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 4 cut(s) 118, 122, 382, 805
BspACI CCGC 1 cut(s) 887
BspANI GGCC 2 cut(s) 452, 695
BspCNI CTCAG 1 cut(s) 669
BspDI ATCGAT 1 cut(s) 121
BspFNI CGCG 1 cut(s) 783
BspLI GGNNCC 3 cut(s) 51, 247, 637
BspOI GCTAGC 1 cut(s) 815
BspPI GGATC 1 cut(s) 126
BspT107I GGYRCC 2 cut(s) 49, 635
BsrDI GCAATG 2 cut(s) 279, 543
BsrFI RCCGGY 2 cut(s) 415, 452
BsrI ACTGG 1 cut(s) 51
BssAI RCCGGY 2 cut(s) 415, 452
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 4 cut(s) 118, 122, 382, 805
BssT1I CCWWGG 1 cut(s) 499
Bst2UI CCWGG 3 cut(s) 40, 640, 882
Bst4CI ACNGT 5 cut(s) 76, 301, 309, 396, 624
BstC8I GCNNGC 5 cut(s) 320, 454, 550, 718, 813
BstDEI CTNAG 4 cut(s) 177, 677, 723, 828
BstF5I GGATG 2 cut(s) 354, 797
BstFNI CGCG 1 cut(s) 783
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 4 cut(s) 121, 125, 385, 808
BstMAI GTCTC 4 cut(s) 307, 336, 860, 906
BstMBI GATC 4 cut(s) 118, 122, 382, 805
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 2 cut(s) 449, 829
BstNI CCWGG 3 cut(s) 40, 640, 882
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 38, 638, 880
BstUI CGCG 1 cut(s) 783
BstXI CCANNNNNNTGG 1 cut(s) 691
Bsu15I ATCGAT 1 cut(s) 121
BsuRI GGCC 2 cut(s) 452, 695
BsuTUI ATCGAT 1 cut(s) 121
BtgZI GCGATG 1 cut(s) 764
BtsCI GGATG 2 cut(s) 354, 797
BtsIMutI CAGTG 2 cut(s) 44, 297
Cac8I GCNNGC 5 cut(s) 320, 454, 550, 718, 813
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 2 cut(s) 415, 452
Cfr13I GGNCC 3 cut(s) 246, 450, 884
ClaI ATCGAT 1 cut(s) 121
Csp6I GTAC 2 cut(s) 148, 496
CspAI ACCGGT 1 cut(s) 415
CviAII CATG 4 cut(s) 326, 386, 587, 713
CviQI GTAC 2 cut(s) 148, 496
DdeI CTNAG 4 cut(s) 177, 677, 723, 828
DpnI GATC 4 cut(s) 120, 124, 384, 807
DpnII GATC 4 cut(s) 118, 122, 382, 805
Eco130I CCWWGG 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 246, 884
Eco47III AGCGCT 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 246
EcoRII CCWGG 3 cut(s) 38, 638, 880
EcoT14I CCWWGG 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 860
FaeI CATG 4 cut(s) 329, 389, 590, 716
FaqI GGGAC 1 cut(s) 444
FatI CATG 4 cut(s) 325, 385, 586, 712
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 428
FokI GGATG 2 cut(s) 341, 784
FspBI CTAG 4 cut(s) 500, 555, 591, 812
GlaI GCGC 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 2 cut(s) 452, 695
HapII CCGG 3 cut(s) 416, 453, 864
HhaI GCGC 1 cut(s) 131
Hin1II CATG 4 cut(s) 329, 389, 590, 716
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 4 cut(s) 217, 563, 643, 945
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 3 cut(s) 416, 453, 864
HphI GGTGA 2 cut(s) 112, 253
Hpy166II GTNNAC 6 cut(s) 236, 295, 392, 399, 429, 496
Hpy188I TCNGA 5 cut(s) 114, 184, 439, 568, 678
Hpy188III TCNNGA 1 cut(s) 755
Hpy8I GTNNAC 6 cut(s) 236, 295, 392, 399, 429, 496
Hpy99I CGWCG 1 cut(s) 474
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 5 cut(s) 76, 301, 309, 396, 624
HpyCH4V TGCA 4 cut(s) 253, 290, 560, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 829
HpyF3I CTNAG 4 cut(s) 177, 677, 723, 828
Hsp92II CATG 4 cut(s) 329, 389, 590, 716
HspAI GCGC 1 cut(s) 129
KroI GCCGGC 1 cut(s) 452
KroNI GCCGGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 382
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 4 cut(s) 118, 122, 382, 805
LmnI GCTCC 1 cut(s) 479
LweI GCATC 2 cut(s) 363, 430
MaeI CTAG 4 cut(s) 500, 555, 591, 812
MaeIII GTNAC 4 cut(s) 362, 418, 463, 660
MalI GATC 4 cut(s) 120, 124, 384, 807
MboI GATC 4 cut(s) 118, 122, 382, 805
MboII GAAGA 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 1 cut(s) 647
MlyI GAGTC 2 cut(s) 572, 637
MnlI CCTC 7 cut(s) 197, 235, 368, 706, 891, 936, 961
MroNI GCCGGC 1 cut(s) 452
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 3 cut(s) 167, 591, 689
MspI CCGG 3 cut(s) 416, 453, 864
MspR9I CCNGG 3 cut(s) 40, 640, 882
MvaI CCWGG 3 cut(s) 40, 640, 882
MvnI CGCG 1 cut(s) 783
MwoI GCNNNNNNNGC 2 cut(s) 449, 829
NaeI GCCGGC 1 cut(s) 454
NdeII GATC 4 cut(s) 118, 122, 382, 805
NgoMIV GCCGGC 1 cut(s) 452
NheI GCTAGC 1 cut(s) 811
NlaIII CATG 4 cut(s) 329, 389, 590, 716
NlaIV GGNNCC 3 cut(s) 51, 247, 637
NmuCI GTSAC 1 cut(s) 418
NspI RCATGY 1 cut(s) 329
PciI ACATGT 1 cut(s) 325
PcsI WCGNNNNNNNCGW 2 cut(s) 103, 358
PdiI GCCGGC 1 cut(s) 454
PfeI GAWTC 2 cut(s) 217, 945
PfoI TCCNGGA 1 cut(s) 880
PinAI ACCGGT 1 cut(s) 415
Ple19I CGATCG 1 cut(s) 125
PleI GAGTC 2 cut(s) 571, 637
PpsI GAGTC 2 cut(s) 571, 637
PpuMI RGGWCCY 1 cut(s) 246
PscI ACATGT 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 3 cut(s) 38, 638, 880
PspGI CCWGG 3 cut(s) 38, 638, 880
PspN4I GGNNCC 3 cut(s) 51, 247, 637
PspPI GGNCC 3 cut(s) 246, 450, 884
PspPPI RGGWCCY 1 cut(s) 246
PvuI CGATCG 1 cut(s) 125
RsaI GTAC 2 cut(s) 149, 497
RsaNI GTAC 2 cut(s) 148, 496
RseI CAYNNNNRTG 3 cut(s) 167, 591, 689
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 4 cut(s) 118, 122, 382, 805
Sau96I GGNCC 3 cut(s) 246, 450, 884
SchI GAGTC 2 cut(s) 572, 637
ScrFI CCNGG 3 cut(s) 40, 640, 882
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 363, 430
SinI GGWCC 2 cut(s) 246, 884
SmiMI CAYNNNNRTG 3 cut(s) 167, 591, 689
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 1 cut(s) 647
SsiI CCGC 1 cut(s) 887
SspMI CTAG 4 cut(s) 500, 555, 591, 812
StyD4I CCNGG 3 cut(s) 38, 638, 880
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 5 cut(s) 76, 301, 309, 396, 624
TaqI TCGA 2 cut(s) 121, 125
TasI AATT 1 cut(s) 647
TfiI GAWTC 2 cut(s) 217, 945
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 51, 304
TseFI GTSAC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 2 cut(s) 701, 781
TspGWI ACGGA 3 cut(s) 221, 350, 866
TspRI CASTG 2 cut(s) 51, 304
VpaK11BI GGWCC 2 cut(s) 246, 884
XceI RCATGY 1 cut(s) 329
XmaJI CCTAGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 428
XspI CTAG 4 cut(s) 500, 555, 591, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.