Rw7G035270

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003077
Physical Location & Seq
Forward (+)
52564542 .. 52565777
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035270.1

Sequence Viewer

Length: 1128 bp
ATGACTCAACCTCCTGGTTTCATTGATCAGTCTCACCCCACTCATGTCTGTAAACTCAACAAATCCCTCTATGGCCTTAAGCAGGCTCCTCGGGCGTGGTTTCAGAGAATGACATCTTTTCTGTTGTCTATTGGGTTTACCCAAAGTTTGGCAGACTCATCCTTATTCATTTTTGCTCATGGTCCGCACACTATTTATTTTTTGTTGTATGTGGATGACATTGTTCACACCTCCTCTCATGGCCTTCACCTTAATCAAGTCAAATATGCTCATGATCTCCTTTCTAAACATGATATGTTGCTTACCAAGCCCATGAGTACCCCTATGTCTGCCAAGGATTCTCTCACTGTCACTGATGGTGTGTTACTTGATAATCCCACTGAATTCCGGACACTCGTCGGTGCTTTACAATACCTCACGATTACACGTCCGGATATTGCTTTTGCGGTCAACTCGGTTTCTCAGTTTATGAGTCAACCTCGTCTTCCTCATCTTGTTGCCGTGAAGCGCATTCTTCGCTATGTCAAAGGCACACTTGGTCATGGTTTATCCTTTGCTCCTCAACATCAGCCAGTGTCCCTCTTCGCCTACTCTGATGCGGATTGGGCGGGCTGTCCCGACTCCCGTCGGTCCACGTCAGGTTATCTTGTGTATCTTGGTTCGAATTTGATCTCTTGGTGCTCCAAGAAGCAGCCCACGATTGCCCGCTCGAGTGCCGAGTCGGAATATCGTTCCCTTGCTCATGCCTGTGCTGAAACTACTTGGTTGGGTTATCTGCTCTATGAACTTGGCGCTCGCATTCAGTTTCCCATCTTATTGCATTGTGATAATCTTAGTGCTACCTACATGGCGTCCAATCCTGTATTCCATGCTCGAACTAAGCATATCGAGCTTGATTATCATTATGTCCGCGAAAAAGTTGCTCTTGGTAGTCATCGGGTCTGCTTCATTCCCTCTGTTGATCAGCCAGCTGATCTACTCACTAAACCACTTCACAAGTCACGTCATCTTCTCCTTTCAAGCAAACTTGTTCATCCGGAGCCGCCAAGTTTGAGGGGGGGTGTTAGAGAGACTAAATCTTCTCCTGAAATCAAGTCATCTAATTCTTCTCCATTGATTTGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs

Protein Analysis

375

Amino Acids

41.84

Weight (kDa)

9.13

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 1 - 99 9.9e-21 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 148
AccBSI CCGCTC 1 cut(s) 708
AccII CGCG 1 cut(s) 912
AccIII TCCGGA 3 cut(s) 387, 430, 1036
AciI CCGC 7 cut(s) 185, 446, 599, 608, 706, 910, 1043
AcsI RAATTY 2 cut(s) 383, 664
AcyI GRCGYC 1 cut(s) 851
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 2 cut(s) 82, 148
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 425
AgsI TTSAA 1 cut(s) 1020
AjiI CACGTC 3 cut(s) 428, 636, 1004
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AluBI AGCT 2 cut(s) 892, 971
AluI AGCT 2 cut(s) 892, 971
Alw21I GWGCWC 1 cut(s) 683
Alw26I GTCTC 2 cut(s) 36, 1064
Ama87I CYCGRG 2 cut(s) 90, 709
Aor13HI TCCGGA 3 cut(s) 387, 430, 1036
AoxI GGCC 2 cut(s) 73, 241
ApeKI GCWGC 1 cut(s) 691
ApoI RAATTY 2 cut(s) 383, 664
AspLEI GCGC 2 cut(s) 510, 794
AspS9I GGNCC 2 cut(s) 182, 630
AsuHPI GGTGA 2 cut(s) 26, 239
AsuII TTCGAA 1 cut(s) 662
AvaI CYCGRG 2 cut(s) 90, 709
AvaII GGWCC 2 cut(s) 182, 630
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 1 cut(s) 703
BccI CCATC 2 cut(s) 350, 818
BceAI ACGGC 1 cut(s) 485
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 902, 936
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 2 cut(s) 25, 961
BcoDI GTCTC 2 cut(s) 36, 1064
BfoI RGCGCY 1 cut(s) 795
BfrI CTTAAG 1 cut(s) 77
BisI GCNGC 2 cut(s) 692, 1043
BlsI GCNGC 2 cut(s) 693, 1044
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 182, 630
BmeT110I CYCGRG 2 cut(s) 90, 709
BmgBI CACGTC 3 cut(s) 428, 636, 1004
BmgT120I GGNCC 2 cut(s) 182, 630
BmiI GGNNCC 2 cut(s) 87, 1041
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 586
BoxI GACNNNNGTC 2 cut(s) 395, 624
BpiI GAAGAC 1 cut(s) 476
BplI GAGNNNNNCTC 2 cut(s) 463, 495
Bpu14I TTCGAA 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 851
BsaJI CCNNGG 2 cut(s) 89, 333
BsaWI WCCGGW 3 cut(s) 387, 430, 1036
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 148
Bse1I ACTGG 1 cut(s) 572
BseAI TCCGGA 3 cut(s) 387, 430, 1036
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 89, 333
BseGI GGATG 3 cut(s) 158, 220, 1033
BseLI CCNNNNNNNGG 2 cut(s) 82, 148
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 1 cut(s) 572
BseRI GAGGAG 3 cut(s) 78, 223, 549
BseXI GCAGC 1 cut(s) 703
Bsh1236I CGCG 1 cut(s) 912
BshFI GGCC 2 cut(s) 75, 243
BsiHKAI GWGCWC 1 cut(s) 683
BsiHKCI CYCGRG 2 cut(s) 90, 709
BsiSI CCGG 3 cut(s) 388, 431, 1037
BslFI GGGAC 2 cut(s) 562, 600
BslI CCNNNNNNNGG 2 cut(s) 82, 148
BsmAI GTCTC 2 cut(s) 36, 1064
BsmFI GGGAC 2 cut(s) 562, 600
BsmI GAATGC 2 cut(s) 510, 798
BsnI GGCC 2 cut(s) 75, 243
BsoBI CYCGRG 2 cut(s) 90, 709
Bsp119I TTCGAA 1 cut(s) 662
Bsp1286I GDGCHC 1 cut(s) 683
Bsp13I TCCGGA 3 cut(s) 387, 430, 1036
Bsp143I GATC 5 cut(s) 25, 274, 669, 961, 973
BspACI CCGC 7 cut(s) 185, 446, 599, 608, 706, 910, 1043
BspANI GGCC 2 cut(s) 75, 243
BspCNI CTCAG 1 cut(s) 475
BspEI TCCGGA 3 cut(s) 387, 430, 1036
BspFNI CGCG 1 cut(s) 912
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 2 cut(s) 87, 1041
BspT104I TTCGAA 1 cut(s) 662
BspTI CTTAAG 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 708
BsrI ACTGG 1 cut(s) 572
BssECI CCNNGG 2 cut(s) 89, 333
BssMI GATC 5 cut(s) 25, 274, 669, 961, 973
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 587
BstACI GRCGYC 1 cut(s) 851
BstAFI CTTAAG 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 5 cut(s) 84, 610, 706, 796, 969
BstDEI CTNAG 3 cut(s) 462, 833, 879
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 3 cut(s) 158, 220, 1033
BstFNI CGCG 1 cut(s) 912
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 2 cut(s) 510, 794
BstKTI GATC 5 cut(s) 28, 277, 672, 964, 976
BstMAI GTCTC 2 cut(s) 36, 1064
BstMBI GATC 5 cut(s) 25, 274, 669, 961, 973
BstMWI GCNNNNNNNGC 5 cut(s) 92, 307, 516, 605, 889
BstNI CCWGG 1 cut(s) 15
BstPAI GACNNNNGTC 2 cut(s) 395, 624
BstSCI CCNGG 1 cut(s) 13
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 1 cut(s) 703
BstV2I GAAGAC 1 cut(s) 476
BsuRI GGCC 2 cut(s) 75, 243
BtrI CACGTC 3 cut(s) 428, 636, 1004
BtsCI GGATG 3 cut(s) 158, 220, 1033
BtsIMutI CAGTG 4 cut(s) 345, 351, 378, 579
Cac8I GCNNGC 5 cut(s) 84, 610, 706, 796, 969
CciI TCATGA 1 cut(s) 271
CfoI GCGC 2 cut(s) 510, 794
Cfr13I GGNCC 2 cut(s) 182, 630
CseI GACGC 1 cut(s) 840
Csp6I GTAC 1 cut(s) 318
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 3 cut(s) 462, 833, 879
DpnI GATC 5 cut(s) 27, 276, 671, 963, 975
DpnII GATC 5 cut(s) 25, 274, 669, 961, 973
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 333
Eco47I GGWCC 2 cut(s) 182, 630
Eco88I CYCGRG 2 cut(s) 90, 709
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FalI AAGNNNNNCTT 4 cut(s) 519, 551, 909, 941
FaqI GGGAC 2 cut(s) 562, 600
FauI CCCGC 2 cut(s) 601, 713
FbaI TGATCA 2 cut(s) 25, 961
Fnu4HI GCNGC 2 cut(s) 692, 1043
FokI GGATG 3 cut(s) 145, 227, 1020
Fsp4HI GCNGC 2 cut(s) 692, 1043
GlaI GCGC 2 cut(s) 509, 793
GluI GCNGC 2 cut(s) 692, 1043
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 2 cut(s) 75, 243
HapII CCGG 3 cut(s) 388, 431, 1037
HgaI GACGC 1 cut(s) 840
HhaI GCGC 2 cut(s) 510, 794
Hin1I GRCGYC 1 cut(s) 851
Hin6I GCGC 2 cut(s) 508, 792
HinP1I GCGC 2 cut(s) 508, 792
HincII GTYRAC 2 cut(s) 451, 476
HindII GTYRAC 2 cut(s) 451, 476
HinfI GANTC 6 cut(s) 4, 155, 338, 472, 620, 719
HpaII CCGG 3 cut(s) 388, 431, 1037
HphI GGTGA 2 cut(s) 26, 239
Hpy166II GTNNAC 6 cut(s) 53, 138, 226, 451, 476, 633
Hpy188I TCNGA 3 cut(s) 105, 595, 724
Hpy188III TCNNGA 7 cut(s) 272, 388, 418, 431, 617, 1037, 1085
Hpy8I GTNNAC 6 cut(s) 53, 138, 226, 451, 476, 633
Hpy99I CGWCG 2 cut(s) 401, 630
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 3 cut(s) 427, 635, 1003
HpyCH4V TGCA 1 cut(s) 820
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 307, 516, 605, 889
HpyF3I CTNAG 3 cut(s) 462, 833, 879
HpySE526I ACGT 3 cut(s) 427, 635, 1003
Hsp92I GRCGYC 1 cut(s) 851
HspAI GCGC 2 cut(s) 508, 792
Kpn2I TCCGGA 3 cut(s) 387, 430, 1036
Ksp22I TGATCA 2 cut(s) 25, 961
Kzo9I GATC 5 cut(s) 25, 274, 669, 961, 973
LmnI GCTCC 4 cut(s) 91, 562, 686, 1039
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 1 cut(s) 586
MaeII ACGT 3 cut(s) 427, 635, 1003
MaeIII GTNAC 3 cut(s) 349, 363, 999
MalI GATC 5 cut(s) 27, 276, 671, 963, 975
MbiI CCGCTC 1 cut(s) 708
MboI GATC 5 cut(s) 25, 274, 669, 961, 973
MboII GAAGA 6 cut(s) 476, 506, 574, 1001, 1071, 1098
MhlI GDGCHC 1 cut(s) 683
MluCI AATT 3 cut(s) 383, 664, 1102
MlyI GAGTC 4 cut(s) 149, 481, 614, 728
MmeI TCCRAC 1 cut(s) 702
MroI TCCGGA 3 cut(s) 387, 430, 1036
MseI TTAA 2 cut(s) 78, 252
MslI CAYNNNNRTG 1 cut(s) 747
MspA1I CMGCKG 1 cut(s) 971
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 3 cut(s) 388, 431, 1037
MspR9I CCNGG 1 cut(s) 15
Mva1269I GAATGC 2 cut(s) 510, 798
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 5 cut(s) 92, 307, 516, 605, 889
NdeII GATC 5 cut(s) 25, 274, 669, 961, 973
NlaIV GGNNCC 2 cut(s) 87, 1041
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 2 cut(s) 349, 999
NspV TTCGAA 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 709
PagI TCATGA 1 cut(s) 271
PctI GAATGC 2 cut(s) 510, 798
PfeI GAWTC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 2 cut(s) 693, 1044
PleI GAGTC 4 cut(s) 149, 480, 614, 727
PpsI GAGTC 4 cut(s) 149, 480, 614, 727
PshAI GACNNNNGTC 2 cut(s) 395, 624
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 87, 1041
PspPI GGNCC 2 cut(s) 182, 630
PspXI VCTCGAGB 1 cut(s) 709
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 2 cut(s) 78, 252
SatI GCNGC 2 cut(s) 692, 1043
Sau3AI GATC 5 cut(s) 25, 274, 669, 961, 973
Sau96I GGNCC 2 cut(s) 182, 630
SchI GAGTC 4 cut(s) 149, 481, 614, 728
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 683
SfaNI GCATC 1 cut(s) 586
Sfr274I CTCGAG 1 cut(s) 709
SfuI TTCGAA 1 cut(s) 662
SinI GGWCC 2 cut(s) 182, 630
SlaI CTCGAG 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 77, 709
SmoI CTYRAG 2 cut(s) 77, 709
Sse9I AATT 3 cut(s) 383, 664, 1102
SsiI CCGC 7 cut(s) 185, 446, 599, 608, 706, 910, 1043
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 3 cut(s) 430, 638, 1006
TaqI TCGA 4 cut(s) 662, 710, 874, 888
TaqII GACCGA 1 cut(s) 618
TasI AATT 3 cut(s) 383, 664, 1102
TauI GCSGC 1 cut(s) 1045
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 2 cut(s) 78, 252
Tru9I TTAA 2 cut(s) 78, 252
TscAI CASTG 4 cut(s) 352, 358, 385, 579
TseFI GTSAC 2 cut(s) 349, 999
TseI GCWGC 1 cut(s) 691
Tsp45I GTSAC 2 cut(s) 349, 999
TspDTI ATGAA 6 cut(s) 10, 157, 798, 937, 1022, 1113
TspRI CASTG 4 cut(s) 352, 358, 385, 579
Van91I CCANNNNNTGG 1 cut(s) 148
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 2 cut(s) 182, 630
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 2 cut(s) 383, 664
XhoI CTCGAG 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.