RchiOBHm_Chr2g0134981
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003077
Physical Location & Seq
Forward (+)
52091995 .. 52093587
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50596

Sequence Viewer

Length: 921 bp
ATGGAACCAACTTGTTATACTCAGGCTTCCAAAGATGCGAATTGGAGAGCTGCCATGACCGAGGAGATTAACGCTTTGTTGAAAAACCATACCTGGTCGCTTGTTCATCCCTCACCATCTCAGCATGTTGTGGGCTGCAAATGGGTGTTTCGCATTAAGCGAAACCCTGATGGCTCAGTAGAGCGTTACAAGGCCCGCCTTGTTGCCAAAGGATTTCATCAACAGCACGGAATCGATTATGGTGAAACTTATAGTCCTGTTATCAAACCTGCTACCATTCGAACCGTTCTATGTGTGGCGGTGTCTCGGGGTTGGTCTCTTCGTCAGCTGGACGTCAAAAATGCCTTCCTACATGGCTATCTACAAGAGGACGTTTACATGACCCAGCCCCCTGGCTTCGTCGATTCCTCTCGGCCCTCCTATGTCTGTAAGCTTCACAAGGCACTTTATGGCCTCAAACAAGCACCCCGTGCTTGGTTTCAACGCATGAGTTCTTTTCTGCTTTCTATTGGTTTTCACCTTAGTAAGGCTGATTCATCCTTATTCATCTATCAACATGGCCTGTATGTGATTTTCTTCTTACTCTATGTTGATGATATTGTTGTCACGGGCAGCAGTGACCGACTTCTTCAAAGTTTTATCGCTGCCTTGGGTCGTGGATTTGACATTAAGGATCTTGGTCCCCTACACTATTTCCTTGGTTTACAGGTCACACCTAATTCCAATGGCCTCCACATCAATCAACTCAAGTATGCTCATGACATTTTAAAGAAGCATGATCTGTTACACAGTCAACCAGTGAGCACCCCACTGTCAGCTAAGACTGTTCTCACCACACATGATGGGGACCTTCTTGCAAATCCCACTGTTTTTCGCGAGATGGTTGGCTCACTACAGTACCTCACTATTTACACGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

306

Amino Acids

34.57

Weight (kDa)

9.21

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 29 - 271 1.3e-67 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 336
Acc36I ACCTGC 1 cut(s) 277
AccII CGCG 1 cut(s) 876
AciI CCGC 2 cut(s) 196, 299
AclWI GGATC 1 cut(s) 681
AcyI GRCGYC 1 cut(s) 333
AdeI CACNNNGTG 1 cut(s) 470
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 2 cut(s) 474, 526
AflIII ACRYGT 1 cut(s) 912
AgsI TTSAA 3 cut(s) 82, 482, 632
AjiI CACGTC 1 cut(s) 915
AjnI CCWGG 2 cut(s) 92, 391
AluBI AGCT 4 cut(s) 50, 328, 433, 818
AluI AGCT 4 cut(s) 50, 328, 433, 818
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 2 cut(s) 309, 321
AlwI GGATC 1 cut(s) 681
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 5 cut(s) 192, 413, 451, 559, 727
ApeKI GCWGC 4 cut(s) 50, 135, 612, 644
AspS9I GGNCC 4 cut(s) 193, 414, 680, 847
AsuHPI GGTGA 4 cut(s) 105, 254, 509, 823
AsuII TTCGAA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 2 cut(s) 680, 847
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
Bbv12I GWGCWC 1 cut(s) 806
BbvI GCAGC 4 cut(s) 37, 122, 624, 631
BccI CCATC 4 cut(s) 124, 164, 836, 874
BciT130I CCWGG 2 cut(s) 94, 393
BcoDI GTCTC 2 cut(s) 309, 321
BfmI CTRYAG 1 cut(s) 893
BfuAI ACCTGC 1 cut(s) 277
BisI GCNGC 4 cut(s) 51, 136, 613, 645
BlsI GCNGC 4 cut(s) 52, 137, 614, 646
Bme1390I CCNGG 2 cut(s) 94, 393
Bme18I GGWCC 2 cut(s) 680, 847
BmeT110I CYCGRG 1 cut(s) 306
BmgBI CACGTC 1 cut(s) 915
BmgT120I GGNCC 4 cut(s) 193, 414, 680, 847
BmiI GGNNCC 3 cut(s) 6, 682, 848
BmrFI CCNGG 2 cut(s) 94, 393
BmsI GCATC 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 731
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 60, 391, 648, 697
Bsc4I CCNNNNNNNGG 2 cut(s) 474, 526
Bse1I ACTGG 1 cut(s) 797
BseBI CCWGG 2 cut(s) 94, 393
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 4 cut(s) 60, 391, 648, 697
BseGI GGATG 2 cut(s) 106, 536
BseLI CCNNNNNNNGG 2 cut(s) 474, 526
BseMII CTCAG 3 cut(s) 35, 134, 189
BseNI ACTGG 1 cut(s) 797
BseRI GAGGAG 1 cut(s) 77
BseXI GCAGC 4 cut(s) 37, 122, 624, 631
BseYI CCCAGC 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 876
BshFI GGCC 5 cut(s) 194, 415, 453, 561, 729
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 806
BsiHKCI CYCGRG 1 cut(s) 306
BslFI GGGAC 2 cut(s) 666, 860
BslI CCNNNNNNNGG 2 cut(s) 474, 526
BsmAI GTCTC 2 cut(s) 309, 321
BsmFI GGGAC 2 cut(s) 666, 860
BsnI GGCC 5 cut(s) 194, 415, 453, 561, 729
Bso31I GGTCTC 1 cut(s) 321
BsoBI CYCGRG 1 cut(s) 306
Bsp119I TTCGAA 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 2 cut(s) 673, 778
Bsp68I TCGCGA 1 cut(s) 876
BspACI CCGC 2 cut(s) 196, 299
BspANI GGCC 5 cut(s) 194, 415, 453, 561, 729
BspCNI CTCAG 3 cut(s) 34, 133, 188
BspDI ATCGAT 1 cut(s) 234
BspFNI CGCG 1 cut(s) 876
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 3 cut(s) 6, 682, 848
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 1 cut(s) 681
BspT104I TTCGAA 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 321
BsrI ACTGG 1 cut(s) 797
BssECI CCNNGG 4 cut(s) 60, 391, 648, 697
BssMI GATC 2 cut(s) 673, 778
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 648, 697
Bst2UI CCWGG 2 cut(s) 94, 393
Bst4CI ACNGT 6 cut(s) 286, 791, 813, 826, 868, 897
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 5 cut(s) 21, 120, 175, 521, 819
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 2 cut(s) 106, 536
BstFNI CGCG 1 cut(s) 876
BstKTI GATC 2 cut(s) 676, 781
BstMAI GTCTC 2 cut(s) 309, 321
BstMBI GATC 2 cut(s) 673, 778
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstNI CCWGG 2 cut(s) 94, 393
BstNSI RCATGY 1 cut(s) 128
BstSCI CCNGG 2 cut(s) 92, 391
BstSFI CTRYAG 1 cut(s) 893
BstUI CGCG 1 cut(s) 876
BstV1I GCAGC 4 cut(s) 37, 122, 624, 631
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 5 cut(s) 194, 415, 453, 561, 729
BsuTUI ATCGAT 1 cut(s) 234
BtrI CACGTC 1 cut(s) 915
BtsCI GGATG 2 cut(s) 106, 536
BtsI GCAGTG 1 cut(s) 622
BtsIMutI CAGTG 4 cut(s) 622, 804, 809, 864
BtuMI TCGCGA 1 cut(s) 876
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 196
CciI TCATGA 1 cut(s) 757
Cfr13I GGNCC 4 cut(s) 193, 414, 680, 847
ClaI ATCGAT 1 cut(s) 234
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 1 cut(s) 898
CviAII CATG 9 cut(s) 55, 125, 353, 379, 487, 557, 758, 776, 839
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 5 cut(s) 21, 120, 175, 521, 819
DpnI GATC 2 cut(s) 675, 780
DpnII GATC 2 cut(s) 673, 778
DraI TTTAAA 1 cut(s) 768
DraIII CACNNNGTG 1 cut(s) 470
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 648, 697
Eco31I GGTCTC 1 cut(s) 321
Eco47I GGWCC 2 cut(s) 680, 847
Eco88I CYCGRG 1 cut(s) 306
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoO109I RGGNCCY 1 cut(s) 847
EcoRII CCWGG 2 cut(s) 92, 391
EcoT14I CCWWGG 2 cut(s) 648, 697
ErhI CCWWGG 2 cut(s) 648, 697
FaeI CATG 9 cut(s) 58, 128, 356, 382, 490, 560, 761, 779, 842
FaqI GGGAC 2 cut(s) 666, 860
FatI CATG 9 cut(s) 54, 124, 352, 378, 486, 556, 757, 775, 838
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 4 cut(s) 51, 136, 613, 645
FokI GGATG 2 cut(s) 93, 523
Fsp4HI GCNGC 4 cut(s) 51, 136, 613, 645
GluI GCNGC 4 cut(s) 51, 136, 613, 645
GsaI CCCAGC 1 cut(s) 388
HaeIII GGCC 5 cut(s) 194, 415, 453, 561, 729
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 9 cut(s) 58, 128, 356, 382, 490, 560, 761, 779, 842
HincII GTYRAC 1 cut(s) 794
HindII GTYRAC 1 cut(s) 794
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 3 cut(s) 231, 404, 533
HphI GGTGA 4 cut(s) 105, 254, 509, 823
Hpy166II GTNNAC 3 cut(s) 376, 704, 794
Hpy188III TCNNGA 3 cut(s) 758, 875, 918
Hpy8I GTNNAC 3 cut(s) 376, 704, 794
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 2 cut(s) 355, 860
HpyCH4III ACNGT 6 cut(s) 286, 791, 813, 826, 868, 897
HpyCH4IV ACGT 3 cut(s) 333, 372, 914
HpyCH4V TGCA 2 cut(s) 138, 857
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 5 cut(s) 21, 120, 175, 521, 819
HpySE526I ACGT 3 cut(s) 333, 372, 914
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 9 cut(s) 58, 128, 356, 382, 490, 560, 761, 779, 842
Kzo9I GATC 2 cut(s) 673, 778
Lsp1109I GCAGC 4 cut(s) 37, 122, 624, 631
LweI GCATC 1 cut(s) 25
MabI ACCWGGT 1 cut(s) 92
MaeII ACGT 3 cut(s) 333, 372, 914
MaeIII GTNAC 5 cut(s) 185, 604, 617, 709, 783
MalI GATC 2 cut(s) 675, 780
MboI GATC 2 cut(s) 673, 778
MboII GAAGA 3 cut(s) 311, 568, 620
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 806
MluCI AATT 2 cut(s) 40, 718
MnlI CCTC 8 cut(s) 55, 121, 361, 418, 427, 464, 740, 911
MseI TTAA 4 cut(s) 69, 156, 669, 767
MspA1I CMGCKG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 94, 393
MvaI CCWGG 2 cut(s) 94, 393
MvnI CGCG 1 cut(s) 876
MwoI GCNNNNNNNGC 1 cut(s) 470
NdeII GATC 2 cut(s) 673, 778
NlaIII CATG 9 cut(s) 58, 128, 356, 382, 490, 560, 761, 779, 842
NlaIV GGNNCC 3 cut(s) 6, 682, 848
NmeAIII GCCGAG 1 cut(s) 391
NmuCI GTSAC 3 cut(s) 604, 617, 709
NruI TCGCGA 1 cut(s) 876
NspI RCATGY 1 cut(s) 128
NspV TTCGAA 1 cut(s) 280
PagI TCATGA 1 cut(s) 757
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PfeI GAWTC 3 cut(s) 231, 404, 533
PkrI GCNGC 4 cut(s) 52, 137, 614, 646
PpuMI RGGWCCY 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 2 cut(s) 92, 391
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 2 cut(s) 92, 391
PspN4I GGNNCC 3 cut(s) 6, 682, 848
PspPI GGNCC 4 cut(s) 193, 414, 680, 847
PspPPI RGGWCCY 1 cut(s) 847
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 328
RruI TCGCGA 1 cut(s) 876
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
SaqAI TTAA 4 cut(s) 69, 156, 669, 767
SatI GCNGC 4 cut(s) 51, 136, 613, 645
Sau3AI GATC 2 cut(s) 673, 778
Sau96I GGNCC 4 cut(s) 193, 414, 680, 847
ScrFI CCNGG 2 cut(s) 94, 393
SduI GDGCHC 1 cut(s) 806
SexAI ACCWGGT 1 cut(s) 92
SfaNI GCATC 1 cut(s) 25
SfcI CTRYAG 1 cut(s) 893
SfuI TTCGAA 1 cut(s) 280
SinI GGWCC 2 cut(s) 680, 847
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
Sse9I AATT 2 cut(s) 40, 718
SsiI CCGC 2 cut(s) 196, 299
StyD4I CCNGG 2 cut(s) 92, 391
StyI CCWWGG 2 cut(s) 648, 697
TaaI ACNGT 6 cut(s) 286, 791, 813, 826, 868, 897
TaiI ACGT 3 cut(s) 336, 375, 917
TaqI TCGA 3 cut(s) 234, 280, 402
TaqII GACCGA 2 cut(s) 74, 636
TasI AATT 2 cut(s) 40, 718
TfiI GAWTC 3 cut(s) 231, 404, 533
Tru1I TTAA 4 cut(s) 69, 156, 669, 767
Tru9I TTAA 4 cut(s) 69, 156, 669, 767
TscAI CASTG 4 cut(s) 622, 804, 816, 871
TseFI GTSAC 3 cut(s) 604, 617, 709
TseI GCWGC 4 cut(s) 50, 135, 612, 644
Tsp45I GTSAC 3 cut(s) 604, 617, 709
TspDTI ATGAA 4 cut(s) 95, 206, 525, 535
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 4 cut(s) 622, 804, 816, 871
VpaK11BI GGWCC 2 cut(s) 680, 847
XagI CCTNNNNNAGG 1 cut(s) 524
XceI RCATGY 1 cut(s) 128
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.