Rroxscaffold_3G00242950
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003077
Physical Location & Seq
Forward (+)
35500133 .. 35502801
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242950.1

Sequence Viewer

Length: 735 bp
ATGGTGACTCGTCTCCAGGACGAGATTGTTCAACCCCGAAAACGTACTGATGGCATGGTTCGCTATCCTCTTCCTCGCCCTCTTATCTCCTTGGTGGAACTTGCTGAACCCACTTGTTACTCCCAGGCTTCTCAGAAATTAGAATGGCGTCAGGCCATGACAGAAGAAGTCAATGCACTACTCAAAAACAACACATGGACCCTTGTGCCTCCCTCTCCTTCTCAGAATGCAGTCGGTTGCAAATGGGTCTTTCGTATCAAGAAGAAATCAGATGGCTCTGTTGAACGATACAAGGCCCGTCTTGTTGCCAAAGGCTTCCATCAACAGCAAGGTATCGATTATGATGAAACATTTAGTCCTGTTATTAAGCCTGCAACTATTCGTACTGTGATTACTATGGCTGTTTCACGAAGTTGGCCTCTTAGGCAATTAGATGTCAAGAATGTCTTCCTTCATGGCATTCTTAAGGAAGACGTGTTCATGGTACAACCTCCTGGCTTTATTGACCCATCAAAACCTCATCATGTCTGCAAACTTAACAAAGCACTTTATAGATTGAAACAGGCACCTCGTGCTTGGTTCCATCGTATGAGCTCATTTCTGTTATCTGCTGGTTTCACTCAAAGTATTGCAGACCCATCTCTATTTATTTATCGTCATGGTCCACATATTATCTATTTTCTCTTATATGTTGATGATATTGTGGTAACTGGGAGTAACAACCACCTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

244

Amino Acids

28.01

Weight (kDa)

9.73

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 63 - 242 2e-53 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 565
AcyI GRCGYC 1 cut(s) 148
AdeI CACNNNGTG 1 cut(s) 572
AfaI GTAC 3 cut(s) 46, 385, 486
AflII CTTAAG 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 3 cut(s) 32, 284, 559
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 3 cut(s) 15, 123, 493
AloI GAACNNNNNNTCC 2 cut(s) 461, 493
AluBI AGCT 1 cut(s) 594
AluI AGCT 1 cut(s) 594
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 1 cut(s) 17
AoxI GGCC 3 cut(s) 153, 294, 416
Asp700I GAANNNNTTC 1 cut(s) 446
AspS9I GGNCC 3 cut(s) 198, 295, 662
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 198, 662
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 596
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 2 cut(s) 439, 477
Bbv12I GWGCWC 1 cut(s) 596
BccI CCATC 6 cut(s) 44, 266, 327, 517, 591, 646
BciT130I CCWGG 3 cut(s) 17, 125, 495
BcoDI GTCTC 1 cut(s) 17
BfrI CTTAAG 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 424
Bme1390I CCNGG 3 cut(s) 17, 125, 495
Bme18I GGWCC 2 cut(s) 198, 662
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 3 cut(s) 198, 295, 662
BmiI GGNNCC 3 cut(s) 200, 567, 581
BmrFI CCNGG 3 cut(s) 17, 125, 495
BmrI ACTGGG 1 cut(s) 720
BmuI ACTGGG 1 cut(s) 720
BpiI GAAGAC 2 cut(s) 439, 477
Bsa29I ATCGAT 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 90, 123
Bse1I ACTGG 1 cut(s) 715
BseBI CCWGG 3 cut(s) 17, 125, 495
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 90, 123
BseMII CTCAG 2 cut(s) 146, 236
BseNI ACTGG 1 cut(s) 715
BshFI GGCC 3 cut(s) 155, 296, 418
BshNI GGYRCC 1 cut(s) 565
BshVI ATCGAT 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 596
BsmAI GTCTC 1 cut(s) 17
BsmBI CGTCTC 1 cut(s) 17
BsmI GAATGC 2 cut(s) 232, 459
BsnI GGCC 3 cut(s) 155, 296, 418
Bsp1286I GDGCHC 1 cut(s) 596
BspANI GGCC 3 cut(s) 155, 296, 418
BspCNI CTCAG 2 cut(s) 145, 235
BspDI ATCGAT 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 200, 567, 581
BspT107I GGYRCC 1 cut(s) 565
BspTI CTTAAG 1 cut(s) 464
BsrI ACTGG 1 cut(s) 715
BssECI CCNNGG 2 cut(s) 90, 123
BssNI GRCGYC 1 cut(s) 148
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 3 cut(s) 17, 125, 495
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 148
BstAFI CTTAAG 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 572
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 132, 222, 422
BstMAI GTCTC 1 cut(s) 17
BstMWI GCNNNNNNNGC 3 cut(s) 60, 424, 572
BstNI CCWGG 3 cut(s) 17, 125, 495
BstSCI CCNGG 3 cut(s) 15, 123, 493
BstV2I GAAGAC 2 cut(s) 439, 477
Bsu15I ATCGAT 1 cut(s) 336
BsuRI GGCC 3 cut(s) 155, 296, 418
BsuTUI ATCGAT 1 cut(s) 336
BtrI CACGTC 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 372
Cfr13I GGNCC 3 cut(s) 198, 295, 662
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 1 cut(s) 137
Csp6I GTAC 3 cut(s) 45, 384, 485
CviAII CATG 7 cut(s) 55, 157, 195, 455, 481, 524, 659
CviQI GTAC 3 cut(s) 45, 384, 485
DdeI CTNAG 3 cut(s) 132, 222, 422
DraIII CACNNNGTG 1 cut(s) 572
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 596
Eco47I GGWCC 2 cut(s) 198, 662
Eco53kI GAGCTC 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 594
EcoRII CCWGG 3 cut(s) 15, 123, 493
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 7 cut(s) 58, 160, 198, 458, 484, 527, 662
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FatI CATG 7 cut(s) 54, 156, 194, 454, 480, 523, 658
FriOI GRGCYC 1 cut(s) 596
HaeIII GGCC 3 cut(s) 155, 296, 418
HgaI GACGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 7 cut(s) 58, 160, 198, 458, 484, 527, 662
HinfI GANTC 1 cut(s) 7
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 3 cut(s) 135, 225, 271
Hpy188III TCNNGA 3 cut(s) 259, 408, 439
Hpy8I GTNNAC 1 cut(s) 665
HpyAV CCTTC 2 cut(s) 228, 461
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 2 cut(s) 43, 474
HpyCH4V TGCA 6 cut(s) 176, 230, 240, 374, 531, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 424, 572
HpyF3I CTNAG 3 cut(s) 132, 222, 422
HpySE526I ACGT 2 cut(s) 43, 474
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 7 cut(s) 58, 160, 198, 458, 484, 527, 662
MaeII ACGT 2 cut(s) 43, 474
MaeIII GTNAC 4 cut(s) 4, 116, 706, 716
MboII GAAGA 5 cut(s) 62, 176, 274, 439, 482
MhlI GDGCHC 1 cut(s) 596
MluCI AATT 2 cut(s) 137, 428
MnlI CCTC 9 cut(s) 78, 84, 90, 219, 223, 429, 501, 528, 579
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 3 cut(s) 366, 465, 537
MspCI CTTAAG 1 cut(s) 464
MspR9I CCNGG 3 cut(s) 17, 125, 495
Mva1269I GAATGC 2 cut(s) 232, 459
MvaI CCWGG 3 cut(s) 17, 125, 495
MwoI GCNNNNNNNGC 3 cut(s) 60, 424, 572
NlaIII CATG 7 cut(s) 58, 160, 198, 458, 484, 527, 662
NlaIV GGNNCC 3 cut(s) 200, 567, 581
NmuCI GTSAC 1 cut(s) 4
PctI GAATGC 2 cut(s) 232, 459
PdmI GAANNNNTTC 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 596
Psp6I CCWGG 3 cut(s) 15, 123, 493
PspGI CCWGG 3 cut(s) 15, 123, 493
PspN4I GGNNCC 3 cut(s) 200, 567, 581
PspPI GGNCC 3 cut(s) 198, 295, 662
RsaI GTAC 3 cut(s) 46, 385, 486
RsaNI GTAC 3 cut(s) 45, 384, 485
SacI GAGCTC 1 cut(s) 596
SaqAI TTAA 3 cut(s) 366, 465, 537
Sau96I GGNCC 3 cut(s) 198, 295, 662
ScrFI CCNGG 3 cut(s) 17, 125, 495
SduI GDGCHC 1 cut(s) 596
SetI ASST 8 cut(s) 46, 334, 477, 493, 520, 571, 596, 729
SinI GGWCC 2 cut(s) 198, 662
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 2 cut(s) 137, 428
SstI GAGCTC 1 cut(s) 596
StyD4I CCNGG 3 cut(s) 15, 123, 493
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 2 cut(s) 46, 477
TaqI TCGA 1 cut(s) 336
TasI AATT 2 cut(s) 137, 428
Tru1I TTAA 3 cut(s) 366, 465, 537
Tru9I TTAA 3 cut(s) 366, 465, 537
TseFI GTSAC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 360, 443, 469
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 198, 662
XmnI GAANNNNTTC 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.