RchiOBHm_Chr6g0310171
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003077
Physical Location & Seq
Forward (+)
67578086 .. 67583538
5453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27893

Sequence Viewer

Length: 2670 bp
ATGCGAACCTATCTCGGTGATTGGGAGATACCGGAGGCGGTTGGCTTTCAGGCTCGTCCTATTCTAAATCAAGGTCTTCTCCCATCTTATATGTCACTCTCATTGCCGTGGGAGCATTGCTTATTCTTGCCTATTCACACAGTGGCAAAGGTGGACTTGGTAATACTAGAGGGGCTATTAGCAGGGTTCAAGGTGTGTGATTACTCATGCACAATGGAGGTCCAACAACCAATTCCTGTTTTGAAAAAAGCATATGGTGACAGCATGCATAAGGTTTTGCACATTGGCCCTGATACTTGTACAGTGGTTTCTAAATTGTGTAGTGAAGAGGAAACTGAAGCCTGGGGTGTGGATCCGTATGACGTAGAGGATGCTGATAGAAGTTGCAAAGCTCTTGTGCGCAAAGGTGGCCTATGCAAAGATGGTCTCTATCCGCTTAGCTTGTCTTCCGAGTCCACGGTTCTCCATGCACTCTCCTCTGTTGACTCGTCTCTTTGGCACAATGGCTTAGGTCATCCCTCCTCTTCCGTTTTAGCTCCTTTAGGATCTACTCTTGGTTCCACACTTTCTTTTAATTCTTTTTGTAATAATTGTGCACTTAGCAAGTCCCATCAATTACCTTTTCCTTCCAACCATATTTCTGCCTCTTCTGTATTTTCTATAATTCATAGTGATGTATGGATGTCTCCTACCTTGTCTGTCACTAGCTTCAAATATTATGTTCTTTTCACAGACGAATACTCTCCTTATACATGGCTTTATCCTATGCACCAGAAAAATGAGGTCCTTACTCACTTTCAAAGCCTTGTTGCCATGATTTGCAATATTTTCCACAATTTTGTACAATATCTTCAAAGTGACAATGATACAGAATATGTCAACAATGTCTTCTCCCAGTTTTGTGCCTCTTCGGGCATCCAACAACGATTTTCTTGTCCATACACTCCACAACAAAATGGGCTTGCTGAAAGAAAACATCGACATATTGCCACTATGGCTCGCACTCTACTTCTCACCTTGGGTGCTCCTCAAAATCTTTGGGCTGATGCTTTTTTTACATCTGTATATCTCATTAACCTTCTTCCCACTCCTACTCTAAATTGGGATACTCCTCACACACGTCTCTATGGTCATCCTCCTCCTTATTCATCCCTTCGTGTTTTTGGTTGCTCATGTTTTCCTATCCTAGGTACAAATGTTCCCAACAAATTGTCTAGTCGTAGTGTCGAGTACAAGCTAATTCATCTTCTGCTACCTTCGAGTTCACTCTTCTCTCCAATCGAGTTAACATTGTACCTCCTCAGGCCATCCTGCCGAGCTCAGTGTCATCTGGCCCACTCCCAACTGATCACCAATTTGAGCAATCTACCCTTCCTTTCTCTCACAGCACAGCCCAATGACCCAAGTCTAAGTTCACAGCCCATTGACCCAATTCCAAGTCTACAGCCCAATGCCCCAAGTTCACACTCTGGTCAAAATCCACCCTTGGCATCTCTCGAATCAGACAGGCACTCCATCAATCTCGTGCTTCCGCTTCCTTCATCACCCGCCCAGCTGCCCTCATCGCAGCCCCCTGGTCGTGCTCCTTTGGTCGCTCCTCCGATCATGACTTACTGCTGCCGTCACCGACCGGTGGATGCGCCTGCTCAGTCCACCATCGCTCGTGCTCCGCATCTCATTGTAGCCAAACGAATTCTCCACTATGTCAAAGGCACACTCGGCCATGGACTCCATTTTAGTCCTCAAAGCCAACCTGCCCACCTTTATGCGTACTCTGTTGTCGATTGGGCCGACTGCCCAGAATCTCGCCGTTCCACTTCCGACTATCTCATTTACCTTGGCAATACCCTTGTCTCTTGGTGTTCCAAAAAGCAACCTACCATTGCTCGCTCCAGTGTAGAATCTGAATATCGATCCCTTGCTCATTCCAGTGCCGAGACAACTTGGTTAGGCTTCTTACTATATGAACTTAGTTTCCATATTCAGTACCCCATTCTGCTTTACTGTGATAACCTAAGTGCCACATACATGGCTTCTAATCCGGTCTTCCACGCTCGCACCAAGCACATCGAGTTAGATTATCACTTTGTTCGTGAAAAGGTAGCTCTTGGGAGTCATCAAGTTCATTTTGTCCCTTCCGTCGATCACCCAGCTGATCTTCTTACTAAACCACTTCACAAGCAGTGCCATAATCTCCTGTGTACCAAACTTGTGCGTCCTGAGCCACCCAGTTTGAAGGGGGGTGTTCGAGAAAATCACTCTCCTCCATTGATTTCCTCTCCACAATTAAAACTGATTGTGCCAAAATCATTTTCTGTTGTCATTGTTTTGGATTCTTTGGATTATTTGTCTGAAAGATACCTCAACAAGACCCTTCTGGATCTGGCTAGGGTATCAGCTGATGGTGTTGTAATCTTTACAGGCAAGATCTTATGTCAACTTGTAAAATCCATTTATCTGTGCTCTCTTGGAAAGCAGAGAGCTAAAGCAGTTGAGGTCTCCAAATTTGGTAGGGTGGCTAAATTGAGGAACAAAGCCTGGTGGACTAAATTTTTTGGGCAGGTTAGCTTAGAAGAGAACGAAGGTGCTAGCAAGAAGTTTAATATTGCTGCAACAAAGATGTCATTTGTCTCAAATTGTCAAGTTTTCCACCTCAGGTCATTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

889

Amino Acids

98.95

Weight (kDa)

8.41

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 142 - 203 8.5e-08 GAG-pre-integrase domain
rve PF00665 220 - 316 6.1e-12 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 401
Acc36I ACCTGC 2 cut(s) 1762, 2551
AccI GTMKAC 1 cut(s) 1441
AciI CCGC 5 cut(s) 38, 434, 1532, 1548, 1670
AclWI GGATC 5 cut(s) 347, 360, 553, 1908, 2388
AcoI YGGCCR 1 cut(s) 1720
AcsI RAATTY 3 cut(s) 1692, 2504, 2549
AcuI CTGAAG 1 cut(s) 357
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 8 cut(s) 301, 843, 1192, 1232, 1295, 1772, 1988, 2203
AfiI CCNNNNNNNGG 2 cut(s) 1187, 1633
AflIII ACRYGT 1 cut(s) 1118
AgeI ACCGGT 1 cut(s) 1630
AgsI TTSAA 7 cut(s) 190, 244, 712, 800, 854, 2236, 2665
AjiI CACGTC 1 cut(s) 1121
AjnI CCWGG 3 cut(s) 341, 1573, 2537
AjuI GAANNNNNNNTTGG 6 cut(s) 216, 248, 1396, 1428, 2296, 2328
AloI GAACNNNNNNTCC 2 cut(s) 1959, 1991
Alw21I GWGCWC 6 cut(s) 598, 1027, 1321, 1585, 1669, 2465
Alw26I GTCTC 8 cut(s) 431, 495, 690, 1127, 1858, 1931, 2503, 2635
Alw44I GTGCAC 1 cut(s) 594
AlwI GGATC 5 cut(s) 347, 360, 553, 1908, 2388
AoxI GGCC 6 cut(s) 286, 409, 1304, 1332, 1720, 1788
ApaLI GTGCAC 1 cut(s) 594
ApeKI GCWGC 4 cut(s) 1555, 1567, 1617, 2609
ApoI RAATTY 3 cut(s) 1692, 2504, 2549
AsiGI ACCGGT 1 cut(s) 1630
AspA2I CCTAGG 1 cut(s) 1186
AspLEI GCGC 2 cut(s) 402, 1642
AspS9I GGNCC 5 cut(s) 220, 287, 784, 1333, 1788
AsuHPI GGTGA 7 cut(s) 29, 269, 1006, 1342, 1536, 1616, 2138
AsuNHI GCTAGC 1 cut(s) 2588
AvaII GGWCC 2 cut(s) 220, 784
AvrII CCTAGG 1 cut(s) 1186
AxyI CCTNAGG 2 cut(s) 1301, 2654
BaeGI GKGCMC 1 cut(s) 598
BaeI ACNNNNGTAYC 2 cut(s) 1182, 1215
BamHI GGATCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 1321
BarI GAAGNNNNNNTAC 2 cut(s) 834, 866
BauI CACGAG 2 cut(s) 1523, 1662
BbsI GAAGAC 4 cut(s) 68, 438, 880, 2038
Bbv12I GWGCWC 6 cut(s) 598, 1027, 1321, 1585, 1669, 2465
BbvI GCAGC 4 cut(s) 1542, 1579, 1604, 2596
BccI CCATC 7 cut(s) 91, 416, 618, 1315, 1523, 1664, 2396
BceAI ACGGC 3 cut(s) 91, 1605, 1794
BciT130I CCWGG 3 cut(s) 343, 1575, 2539
BciVI GTATCC 1 cut(s) 1099
BclI TGATCA 1 cut(s) 1347
BcoDI GTCTC 8 cut(s) 431, 495, 690, 1127, 1858, 1931, 2503, 2635
BfaI CTAG 6 cut(s) 167, 705, 1187, 1215, 2388, 2589
BfmI CTRYAG 1 cut(s) 1442
BfuAI ACCTGC 2 cut(s) 1762, 2551
BfuI GTATCC 1 cut(s) 1099
BglI GCCNNNNNGGC 1 cut(s) 995
BglII AGATCT 1 cut(s) 2427
BisI GCNGC 4 cut(s) 1556, 1568, 1618, 2610
BlnI CCTAGG 1 cut(s) 1186
BlpI GCTNAGC 1 cut(s) 437
BlsI GCNGC 4 cut(s) 1557, 1569, 1619, 2611
Bme1390I CCNGG 3 cut(s) 343, 1575, 2539
Bme18I GGWCC 2 cut(s) 220, 784
BmgBI CACGTC 1 cut(s) 1121
BmgT120I GGNCC 5 cut(s) 220, 287, 784, 1333, 1788
BmiI GGNNCC 2 cut(s) 354, 559
BmrFI CCNGG 3 cut(s) 343, 1575, 2539
BmrI ACTGGG 2 cut(s) 889, 2223
BmsI GCATC 6 cut(s) 361, 924, 1036, 1499, 1627, 1681
BmtI GCTAGC 1 cut(s) 2592
BmuI ACTGGG 2 cut(s) 889, 2223
BoxI GACNNNNGTC 1 cut(s) 1404
BpiI GAAGAC 4 cut(s) 68, 438, 880, 2038
BpmI CTGGAG 1 cut(s) 1876
Bpu10I CCTNAGC 2 cut(s) 508, 2220
Bpu1102I GCTNAGC 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 1912
BsaI GGTCTC 2 cut(s) 431, 2503
BsaJI CCNNGG 9 cut(s) 107, 342, 456, 1017, 1186, 1485, 1573, 1723, 1837
BsaWI WCCGGW 3 cut(s) 31, 1630, 2041
BsaXI ACNNNNNCTCC 4 cut(s) 1496, 1526, 1680, 1710
Bsc4I CCNNNNNNNGG 2 cut(s) 1187, 1633
Bse118I RCCGGY 1 cut(s) 1630
Bse1I ACTGG 4 cut(s) 895, 1893, 1929, 2229
Bse21I CCTNAGG 2 cut(s) 1301, 2654
Bse3DI GCAATG 3 cut(s) 101, 115, 1881
BseBI CCWGG 3 cut(s) 343, 1575, 2539
BseCI ATCGAT 1 cut(s) 1912
BseDI CCNNGG 9 cut(s) 107, 342, 456, 1017, 1186, 1485, 1573, 1723, 1837
BseGI GGATG 8 cut(s) 376, 514, 687, 915, 1132, 1148, 1307, 1642
BseLI CCNNNNNNNGG 2 cut(s) 1187, 1633
BseMI GCAATG 3 cut(s) 101, 115, 1881
BseMII CTCAG 5 cut(s) 1315, 1334, 1661, 2211, 2668
BseNI ACTGG 4 cut(s) 895, 1893, 1929, 2229
BseRI GAGGAG 8 cut(s) 466, 511, 1017, 1101, 1128, 1289, 1587, 2253
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 4 cut(s) 1542, 1579, 1604, 2596
BseYI CCCAGC 2 cut(s) 1551, 2149
Bsh1285I CGRYCG 1 cut(s) 1631
BshFI GGCC 6 cut(s) 288, 411, 1306, 1334, 1722, 1790
BshTI ACCGGT 1 cut(s) 1630
BshVI ATCGAT 1 cut(s) 1912
BsiEI CGRYCG 1 cut(s) 1631
BsiHKAI GWGCWC 6 cut(s) 598, 1027, 1321, 1585, 1669, 2465
BsiSI CCGG 3 cut(s) 32, 1631, 2042
BslFI GGGAC 2 cut(s) 592, 2117
BslI CCNNNNNNNGG 2 cut(s) 1187, 1633
BsmAI GTCTC 8 cut(s) 431, 495, 690, 1127, 1858, 1931, 2503, 2635
BsmBI CGTCTC 2 cut(s) 495, 1127
BsmFI GGGAC 2 cut(s) 592, 2117
BsnI GGCC 6 cut(s) 288, 411, 1306, 1334, 1722, 1790
Bso31I GGTCTC 2 cut(s) 431, 2503
Bsp1286I GDGCHC 6 cut(s) 598, 1027, 1321, 1585, 1669, 2465
Bsp1407I TGTACA 2 cut(s) 299, 841
Bsp143I GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
Bsp1720I GCTNAGC 1 cut(s) 437
Bsp19I CCATGG 1 cut(s) 1723
BspACI CCGC 5 cut(s) 38, 434, 1532, 1548, 1670
BspANI GGCC 6 cut(s) 288, 411, 1306, 1334, 1722, 1790
BspCNI CTCAG 5 cut(s) 1314, 1333, 1660, 2212, 2667
BspDI ATCGAT 1 cut(s) 1912
BspHI TCATGA 1 cut(s) 1605
BspLI GGNNCC 2 cut(s) 354, 559
BspMI ACCTGC 2 cut(s) 1762, 2551
BspOI GCTAGC 1 cut(s) 2592
BspPI GGATC 5 cut(s) 347, 360, 553, 1908, 2388
BspTNI GGTCTC 2 cut(s) 431, 2503
BsrDI GCAATG 3 cut(s) 101, 115, 1881
BsrFI RCCGGY 1 cut(s) 1630
BsrGI TGTACA 2 cut(s) 299, 841
BsrI ACTGG 4 cut(s) 895, 1893, 1929, 2229
BssAI RCCGGY 1 cut(s) 1630
BssECI CCNNGG 9 cut(s) 107, 342, 456, 1017, 1186, 1485, 1573, 1723, 1837
BssMI GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
BssSI CACGAG 2 cut(s) 1523, 1662
BssT1I CCWWGG 5 cut(s) 1017, 1186, 1485, 1723, 1837
Bst2BI CACGAG 2 cut(s) 1523, 1662
Bst2UI CCWGG 3 cut(s) 343, 1575, 2539
Bst4CI ACNGT 4 cut(s) 142, 304, 460, 2006
Bst6I CTCTTC 6 cut(s) 321, 529, 652, 913, 1274, 2568
BstAUI TGTACA 2 cut(s) 299, 841
BstC8I GCNNGC 7 cut(s) 266, 963, 1000, 1644, 1888, 2056, 2590
BstDSI CCRYGG 3 cut(s) 107, 456, 1723
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstF5I GGATG 8 cut(s) 376, 514, 687, 915, 1132, 1148, 1307, 1642
BstHHI GCGC 2 cut(s) 402, 1642
BstKTI GATC 9 cut(s) 355, 548, 1350, 1605, 1916, 2146, 2158, 2383, 2430
BstMAI GTCTC 8 cut(s) 431, 495, 690, 1127, 1858, 1931, 2503, 2635
BstMBI GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
BstMCI CGRYCG 1 cut(s) 1631
BstMWI GCNNNNNNNGC 6 cut(s) 112, 408, 995, 1564, 1719, 2221
BstNI CCWGG 3 cut(s) 343, 1575, 2539
BstNSI RCATGY 1 cut(s) 268
BstPAI GACNNNNGTC 1 cut(s) 1404
BstSCI CCNGG 3 cut(s) 341, 1573, 2537
BstSFI CTRYAG 1 cut(s) 1442
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 4 cut(s) 1542, 1579, 1604, 2596
BstV2I GAAGAC 4 cut(s) 68, 438, 880, 2038
BstX2I RGATCY 4 cut(s) 352, 545, 2380, 2427
BstXI CCANNNNNNTGG 1 cut(s) 2029
BstYI RGATCY 4 cut(s) 352, 545, 2380, 2427
Bsu15I ATCGAT 1 cut(s) 1912
Bsu36I CCTNAGG 2 cut(s) 1301, 2654
BsuI GTATCC 1 cut(s) 1099
BsuRI GGCC 6 cut(s) 288, 411, 1306, 1334, 1722, 1790
BsuTUI ATCGAT 1 cut(s) 1912
BtgI CCRYGG 3 cut(s) 107, 456, 1723
BtgZI GCGATG 2 cut(s) 1548, 1642
BtrI CACGTC 1 cut(s) 1121
BtsCI GGATG 8 cut(s) 376, 514, 687, 915, 1132, 1148, 1307, 1642
BtsI GCAGTG 1 cut(s) 2189
BtsIMutI CAGTG 6 cut(s) 147, 309, 1328, 1900, 1936, 2189
BveI ACCTGC 2 cut(s) 1762, 2551
Cac8I GCNNGC 7 cut(s) 266, 963, 1000, 1644, 1888, 2056, 2590
CciI TCATGA 1 cut(s) 1605
CfoI GCGC 2 cut(s) 402, 1642
Cfr10I RCCGGY 1 cut(s) 1630
Cfr13I GGNCC 5 cut(s) 220, 287, 784, 1333, 1788
ClaI ATCGAT 1 cut(s) 1912
CseI GACGC 1 cut(s) 2204
Csp6I GTAC 8 cut(s) 300, 842, 1191, 1231, 1294, 1771, 1987, 2202
CspAI ACCGGT 1 cut(s) 1630
CspCI CAANNNNNGTGG 2 cut(s) 2214, 2249
CviAII CATG 9 cut(s) 207, 265, 467, 753, 814, 1173, 1606, 1724, 2029
CviQI GTAC 8 cut(s) 300, 842, 1191, 1231, 1294, 1771, 1987, 2202
DpnI GATC 9 cut(s) 354, 547, 1349, 1604, 1915, 2145, 2157, 2382, 2429
DpnII GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
DraIII CACNNNGTG 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 1720
Eam1104I CTCTTC 6 cut(s) 321, 529, 652, 913, 1274, 2568
EarI CTCTTC 6 cut(s) 321, 529, 652, 913, 1274, 2568
Ecl136II GAGCTC 1 cut(s) 1319
Eco130I CCWWGG 5 cut(s) 1017, 1186, 1485, 1723, 1837
Eco24I GRGCYC 1 cut(s) 1321
Eco31I GGTCTC 2 cut(s) 431, 2503
Eco47I GGWCC 2 cut(s) 220, 784
Eco53kI GAGCTC 1 cut(s) 1319
Eco57I CTGAAG 1 cut(s) 357
Eco81I CCTNAGG 2 cut(s) 1301, 2654
EcoICRI GAGCTC 1 cut(s) 1319
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoO109I RGGNCCY 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 1692
EcoRII CCWGG 3 cut(s) 341, 1573, 2537
EcoT14I CCWWGG 5 cut(s) 1017, 1186, 1485, 1723, 1837
EcoT22I ATGCAT 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 1321
ErhI CCWWGG 5 cut(s) 1017, 1186, 1485, 1723, 1837
Esp3I CGTCTC 2 cut(s) 495, 1127
FaeI CATG 9 cut(s) 210, 268, 470, 756, 817, 1176, 1609, 1727, 2032
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 2 cut(s) 592, 2117
FatI CATG 9 cut(s) 206, 264, 466, 752, 813, 1172, 1605, 1723, 2028
FauI CCCGC 1 cut(s) 1555
FauNDI CATATG 1 cut(s) 253
FbaI TGATCA 1 cut(s) 1347
FblI GTMKAC 1 cut(s) 1441
Fnu4HI GCNGC 4 cut(s) 1556, 1568, 1618, 2610
FokI GGATG 8 cut(s) 383, 501, 694, 902, 1119, 1135, 1294, 1649
FriOI GRGCYC 1 cut(s) 1321
Fsp4HI GCNGC 4 cut(s) 1556, 1568, 1618, 2610
FspBI CTAG 6 cut(s) 167, 705, 1187, 1215, 2388, 2589
FspI TGCGCA 1 cut(s) 401
GlaI GCGC 2 cut(s) 401, 1641
GluI GCNGC 4 cut(s) 1556, 1568, 1618, 2610
GsaI CCCAGC 2 cut(s) 1555, 2153
GsuI CTGGAG 1 cut(s) 1876
HaeIII GGCC 6 cut(s) 288, 411, 1306, 1334, 1722, 1790
HapII CCGG 3 cut(s) 32, 1631, 2042
HgaI GACGC 1 cut(s) 2204
HhaI GCGC 2 cut(s) 402, 1642
Hin1II CATG 9 cut(s) 210, 268, 470, 756, 817, 1176, 1609, 1727, 2032
Hin6I GCGC 2 cut(s) 400, 1640
HinP1I GCGC 2 cut(s) 400, 1640
HincII GTYRAC 4 cut(s) 484, 880, 1287, 2438
HindII GTYRAC 4 cut(s) 484, 880, 1287, 2438
HinfI GANTC 8 cut(s) 452, 485, 1499, 1728, 1802, 1901, 2113, 2333
HpaI GTTAAC 1 cut(s) 1287
HpaII CCGG 3 cut(s) 32, 1631, 2042
HphI GGTGA 7 cut(s) 29, 269, 1006, 1342, 1536, 1616, 2138
Hpy188I TCNGA 6 cut(s) 451, 1504, 1602, 1822, 1906, 2353
Hpy188III TCNNGA 6 cut(s) 1496, 1606, 2093, 2219, 2249, 2378
Hpy99I CGWCG 1 cut(s) 2144
HpyCH4III ACNGT 4 cut(s) 142, 304, 460, 2006
HpyCH4IV ACGT 2 cut(s) 363, 1120
HpyF10VI GCNNNNNNNGC 6 cut(s) 112, 408, 995, 1564, 1719, 2221
HpySE526I ACGT 2 cut(s) 363, 1120
Hsp92II CATG 9 cut(s) 210, 268, 470, 756, 817, 1176, 1609, 1727, 2032
HspAI GCGC 2 cut(s) 400, 1640
Ksp22I TGATCA 1 cut(s) 1347
KspAI GTTAAC 1 cut(s) 1287
Kzo9I GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
LmnI GCTCC 7 cut(s) 112, 541, 1030, 1588, 1600, 1672, 1895
Lsp1109I GCAGC 4 cut(s) 1542, 1579, 1604, 2596
LweI GCATC 6 cut(s) 361, 924, 1036, 1499, 1627, 1681
MaeI CTAG 6 cut(s) 167, 705, 1187, 1215, 2388, 2589
MaeII ACGT 2 cut(s) 363, 1120
MaeIII GTNAC 5 cut(s) 93, 257, 700, 857, 1622
MalI GATC 9 cut(s) 354, 547, 1349, 1604, 1915, 2145, 2157, 2382, 2429
MboI GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
MfeI CAATTG 1 cut(s) 2665
MflI RGATCY 4 cut(s) 352, 545, 2380, 2427
MhlI GDGCHC 6 cut(s) 598, 1027, 1321, 1585, 1669, 2465
MlyI GAGTC 4 cut(s) 461, 479, 1722, 2122
MmeI TCCRAC 4 cut(s) 247, 654, 943, 1845
Mph1103I ATGCAT 1 cut(s) 270
MseI TTAA 5 cut(s) 573, 1074, 1286, 2288, 2601
MslI CAYNNNNRTG 5 cut(s) 106, 672, 1764, 1929, 2027
MspA1I CMGCKG 3 cut(s) 1555, 2153, 2399
MspI CCGG 3 cut(s) 32, 1631, 2042
MspR9I CCNGG 3 cut(s) 343, 1575, 2539
MunI CAATTG 1 cut(s) 2665
MvaI CCWGG 3 cut(s) 343, 1575, 2539
MwoI GCNNNNNNNGC 6 cut(s) 112, 408, 995, 1564, 1719, 2221
NcoI CCATGG 1 cut(s) 1723
NdeI CATATG 1 cut(s) 253
NdeII GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
NheI GCTAGC 1 cut(s) 2588
NlaIII CATG 9 cut(s) 210, 268, 470, 756, 817, 1176, 1609, 1727, 2032
NlaIV GGNNCC 2 cut(s) 354, 559
NmeAIII GCCGAG 3 cut(s) 1340, 1698, 1960
NmuCI GTSAC 5 cut(s) 93, 257, 700, 857, 1622
NsbI TGCGCA 1 cut(s) 401
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 1 cut(s) 268
PaeI GCATGC 1 cut(s) 268
PagI TCATGA 1 cut(s) 1605
PcsI WCGNNNNNNNCGW 1 cut(s) 1788
PfeI GAWTC 4 cut(s) 1499, 1802, 1901, 2333
PinAI ACCGGT 1 cut(s) 1630
PkrI GCNGC 4 cut(s) 1557, 1569, 1619, 2611
PleI GAGTC 4 cut(s) 460, 479, 1722, 2121
PpsI GAGTC 4 cut(s) 460, 479, 1722, 2121
PpuMI RGGWCCY 1 cut(s) 784
PshAI GACNNNNGTC 1 cut(s) 1404
Psp124BI GAGCTC 1 cut(s) 1321
Psp5II RGGWCCY 1 cut(s) 784
Psp6I CCWGG 3 cut(s) 341, 1573, 2537
PspFI CCCAGC 2 cut(s) 1551, 2149
PspGI CCWGG 3 cut(s) 341, 1573, 2537
PspN4I GGNNCC 2 cut(s) 354, 559
PspPI GGNCC 5 cut(s) 220, 287, 784, 1333, 1788
PspPPI RGGWCCY 1 cut(s) 784
PsuI RGATCY 4 cut(s) 352, 545, 2380, 2427
PvuII CAGCTG 3 cut(s) 1555, 2153, 2399
RsaI GTAC 8 cut(s) 301, 843, 1192, 1232, 1295, 1772, 1988, 2203
RsaNI GTAC 8 cut(s) 300, 842, 1191, 1231, 1294, 1771, 1987, 2202
RseI CAYNNNNRTG 5 cut(s) 106, 672, 1764, 1929, 2027
SacI GAGCTC 1 cut(s) 1321
SaqAI TTAA 5 cut(s) 573, 1074, 1286, 2288, 2601
SatI GCNGC 4 cut(s) 1556, 1568, 1618, 2610
Sau3AI GATC 9 cut(s) 352, 545, 1347, 1602, 1913, 2143, 2155, 2380, 2427
Sau96I GGNCC 5 cut(s) 220, 287, 784, 1333, 1788
SchI GAGTC 4 cut(s) 461, 479, 1722, 2122
ScrFI CCNGG 3 cut(s) 343, 1575, 2539
SduI GDGCHC 6 cut(s) 598, 1027, 1321, 1585, 1669, 2465
SfaNI GCATC 6 cut(s) 361, 924, 1036, 1499, 1627, 1681
SfcI CTRYAG 1 cut(s) 1442
SinI GGWCC 2 cut(s) 220, 784
SmiMI CAYNNNNRTG 5 cut(s) 106, 672, 1764, 1929, 2027
SphI GCATGC 1 cut(s) 268
SsiI CCGC 5 cut(s) 38, 434, 1532, 1548, 1670
SspI AATATT 3 cut(s) 716, 826, 2605
SspMI CTAG 6 cut(s) 167, 705, 1187, 1215, 2388, 2589
SstI GAGCTC 1 cut(s) 1321
StyD4I CCNGG 3 cut(s) 341, 1573, 2537
StyI CCWWGG 5 cut(s) 1017, 1186, 1485, 1723, 1837
TaaI ACNGT 4 cut(s) 142, 304, 460, 2006
TaiI ACGT 2 cut(s) 366, 1123
TatI WGTACW 3 cut(s) 299, 841, 1230
TfiI GAWTC 4 cut(s) 1499, 1802, 1901, 2333
Tru1I TTAA 5 cut(s) 573, 1074, 1286, 2288, 2601
Tru9I TTAA 5 cut(s) 573, 1074, 1286, 2288, 2601
TscAI CASTG 6 cut(s) 147, 309, 1328, 1900, 1936, 2189
TseFI GTSAC 5 cut(s) 93, 257, 700, 857, 1622
TseI GCWGC 4 cut(s) 1555, 1567, 1617, 2609
Tsp45I GTSAC 5 cut(s) 93, 257, 700, 857, 1622
TspDTI ATGAA 6 cut(s) 656, 1137, 1232, 1530, 1980, 2114
TspGWI ACGGA 3 cut(s) 345, 517, 2128
TspRI CASTG 6 cut(s) 147, 309, 1328, 1900, 1936, 2189
VneI GTGCAC 1 cut(s) 594
VpaK11BI GGWCC 2 cut(s) 220, 784
XagI CCTNNNNNAGG 1 cut(s) 1185
XapI RAATTY 3 cut(s) 1692, 2504, 2549
XceI RCATGY 1 cut(s) 268
XmaJI CCTAGG 1 cut(s) 1186
XmiI GTMKAC 1 cut(s) 1441
XspI CTAG 6 cut(s) 167, 705, 1187, 1215, 2388, 2589
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.