RchiOBHm_Chr7g0182281
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003077
Physical Location & Seq
Forward (+)
3594001 .. 3597137
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16261

Sequence Viewer

Length: 2805 bp
ATGTCTCCCACTTCGTCAGTTAGTGGTTTTCGATACTATGTCCTATTTACAGATGAGTATTCTCGCTTTACTTGGGTCTATCCTATGCGCCGTAAAAATGAGGTTTTTACTCATTTTCAGTCCTTGGTTGTCATGATACAAAATCACTTTCACAGTTCCATAAAATTTTTACAAAGTGATAATGGCACGGAATACGTCACTAATGCCTTTTCTCATTATTGCAAATCTCTAGGCATTCTCCAACGTTTTTCCTGTCCTCATACGCCTCAACAAAATGGCCTTGCTGAGCGCAAACATCAACATATAGCCACAATGACCCGAACTCTTCTCCTCACATCAGGGGTTCCTCACAATCTGTGGGTAGAAGCTGTCCTAACCTCAGTCTATCTTATCAATCTCCTTCCTACACCAGTTTTCGATTGGGCAACACCTTATTCTTGTCTCTATGGACGCTCTCCCCCCTATTCTTCTCTTCGTGTTTTTGGGTGTTCTTGCTTCCCTTATCTTGGTCCCTATGTCTCAGACAAGTTATCCAGTCGTAGTATAGAATGTGTCTTTCTTGGTTATAGTCCTAATCATAAGGGCTATCGGTGTCTCAATCCAAAAACTGGTCGTGTCTATGTGTCACGTAACGTTTTATTTAATGAAAATAGTTTCCCTTTTAAAGATTTGCAGGTACCATCAAATGTTCCTCCTGGTCCACTTCACTTGTTCACATTATCTAGCCCATTGACTTTTCCACCAGAAACAGTCCCTACATCGATCAGTCCCCCTGCTTCAGATCCTCCTACGACCCTACAACTGCTAAACAGCTCCATACCTTCCGCTCAAGCATCTCCATGCAACTCCGACTTCCCACTGTCAATTCCCGCAGGTGACTCCACCGTCACGCCTCCACCCTCACCCCCGTCCGCCCGAACTCCCCTTCTCCAATACACCCGCCGTCGCCCAACGACTGCGCCAGCCACAGCGCCGCTCTCCCCTCCATCTCCGGCCACCGCGTCGCGCAACGCAGCAGTGCCGCCTATCTCTCCTCCTCCAGCCTCCGCGTCCCCCGACGCAGCTTCCCATCCTTCGTCATCCTCACTGCCTGCGGCACCTTCCTCTCCTCTTTCTAATTCACCAGCTCCTTCTCCTGATCCACCGGTCAATGCTCCTCCACCGGCCCCCGCCCCATCGCATCCAATGGTCACCCGACTTCGGGATGGCATTGTCCAAACCCGTGCACTAACAGATGGTACAGCCTCTCAGAAACCTGAATGGCGTGCTGCCATGACAGAGGAGATCAATGCCTTGTTGAAAAACAACACATGGACTCTAGTGCCTCCTTCTTCTTCACAAAATACAGTGGGTTGCAAATGGGTTTTTCGGGTGAAGCGGAAGTCTGACGGATCTATTGAGCGGTACAAGGCTCGTCTTGTCGCCAAGGGCTTCCATCAACAGCAAGGTATTGATTACGGTGAAACTTATAGTCCAGTCATTAAGCCTGCAACTATTCGTACAGTCCTTTCGATTGCTGTGTCTCGTGGTTGGTCCCTACGCCAATTAGATGTTAAAAATGCTTTCCTTCATGGTGTTCTTACTGAGGATGTGTATATGGTTCAGCCCCCTGGTTTTGTTGATCAGTCTAGGCCTAGTCATGTCTGCAAATTAAACAAAGCTCTTTATGGCTTGAAACAGGCACCTCGTGCATGGTTTCAGCGTATGAGCACCTTTCTTCTTTCTGTTGGGTTCTCTCAGAGTCTTGCAGATTCCTCTTTGTTTATCTATCGTCATGGCCAGCATCAGATATTTTTTCTGCTATATGTAGATGACATTGTTGTTACGGGCAGCAATGTTCAGCTGCTTCAATCCTTTATTGATAACCTTGGGCGTGGTTTTGACATTAAGGATCTAGGACCTTTGCATTATTTTTTGGGATTACAGGTTACAAAACACTATCATGGGCTGCATATTAATCAGCTTAAGTATGCTCATGATCTTCTCTCTAAACATGACCTTTTGCTTAGCAAGCCTGTCAGTACGCCCATGTCTGCCAAGTCTGATCTTCACTCTACTGATGGGGTTTTCCTTGAGAATCCTACATTGTATCGTGAGCTTGTTGGGTCTTTGCAATATCTCACAATCACCCGTCCTGACATTGCTTTTGCTGTTAACTTGGTCTCTCAATTTATGAGTCAGCCTCGTGTTTCCCATCTCATTGCTGTAAAACGTATCCTTCGTTACGTCAAGGGCTCACTTGGTCATGGGTTGTTGTTCACTCCTCAACGACAACCAGTTCATCTTTCAGCTTACTCTGATGCAGATTGGGCTGGTTGTCCAACGTCTCGTCGGTCTACATCTGGCTACCTTGTGTATCTCGGTTCCAATCTCATCTCTTGGTGTTCTAAAAAGCAACCTACGGTTGCACGTTCTAGTGCTGAGTCTGAATACAGAGCTCTGGCTCATGCTAGTGCTGAAACGACATGGTTGGGTTATTTATTGTATGAGCTTGGTGCCACGATCCATTTTCCAATTTTACTTCATTGTGATAATCTCAGCACCACATATATGGCTTCCAATCCGGTGTTCCATGCCCGCACCAAGCACATTGAGCTCGACTACCACTATGTGCGTGAAAAAGTTGCTCGTGGAAGTCACAGAGTTTGCTTCATTCCTTCCAGAGATCAGCCAGCTGACTTACTCACAAAGCCACTTCACAAGTCTCGACATCTTCTTTTCACCAGCAAACTTGTCCGTCCAGGCCGGTCAAGTTTGAGGGGGGATGTTAAAGATACTCAATCTTCCTCAATCACTAGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

934

Amino Acids

103.48

Weight (kDa)

9.54

Isoelectric Point (pI)

58.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 10 - 89 2.2e-11 Integrase core domain
SH3_retrovirus PF25597 163 - 223 1.4e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 435 - 678 7.1e-70 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 863
AasI GACNNNNNNGTC 1 cut(s) 884
Acc36I ACCTGC 2 cut(s) 664, 863
Acc65I GGTACC 1 cut(s) 676
AccB1I GGYRCC 4 cut(s) 676, 1096, 1681, 2495
AccB7I CCANNNNNTGG 1 cut(s) 608
AccBSI CCGCTC 3 cut(s) 827, 976, 1402
AccI GTMKAC 1 cut(s) 2336
AccII CGCG 3 cut(s) 1001, 1006, 1049
AclI AACGTT 2 cut(s) 244, 633
AclWI GGATC 5 cut(s) 776, 1133, 1399, 1899, 2497
AcoI YGGCCR 2 cut(s) 993, 1777
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 1 cut(s) 762
AdeI CACNNNGTG 2 cut(s) 1688, 2611
AfaI GTAC 5 cut(s) 678, 1240, 1406, 1501, 2023
AfiI CCNNNNNNNGG 4 cut(s) 506, 608, 1200, 1201
AflII CTTAAG 1 cut(s) 1964
AgeI ACCGGT 1 cut(s) 1144
AgsI TTSAA 3 cut(s) 1300, 1675, 1850
AjnI CCWGG 3 cut(s) 694, 1609, 2740
AjuI GAANNNNNNNTTGG 2 cut(s) 2552, 2584
Alw21I GWGCWC 4 cut(s) 1228, 1712, 2440, 2598
Alw26I GTCTC 8 cut(s) 9, 446, 523, 599, 1527, 2167, 2331, 2709
Alw44I GTGCAC 1 cut(s) 1224
AlwI GGATC 5 cut(s) 776, 1133, 1399, 1899, 2497
AoxI GGCC 6 cut(s) 277, 993, 1164, 1631, 1777, 2743
ApaLI GTGCAC 1 cut(s) 1224
ApeKI GCWGC 6 cut(s) 1013, 1061, 1268, 1830, 1843, 1948
ApoI RAATTY 1 cut(s) 164
ArsI GACNNNNNNTTYG 2 cut(s) 2129, 2161
AseI ATTAAT 1 cut(s) 1956
AsiGI ACCGGT 1 cut(s) 1144
Asp718I GGTACC 1 cut(s) 676
AspLEI GCGC 5 cut(s) 90, 291, 961, 973, 1008
AspS9I GGNCC 5 cut(s) 509, 698, 1165, 1533, 1898
AsuHPI GGTGA 8 cut(s) 887, 894, 1113, 1183, 1384, 1472, 2119, 2713
AvaII GGWCC 4 cut(s) 509, 698, 1533, 1898
BaeGI GKGCMC 1 cut(s) 1228
BalI TGGCCA 1 cut(s) 1779
BanI GGYRCC 4 cut(s) 676, 1096, 1681, 2495
BanII GRGCYC 3 cut(s) 2237, 2440, 2598
BauI CACGAG 4 cut(s) 1524, 1686, 2184, 2628
Bbv12I GWGCWC 4 cut(s) 1228, 1712, 2440, 2598
BbvI GCAGC 6 cut(s) 1025, 1073, 1255, 1830, 1842, 1935
BccI CCATC 9 cut(s) 688, 994, 1077, 1183, 1199, 1229, 1443, 2054, 2202
BceAI ACGGC 2 cut(s) 75, 927
BciT130I CCWGG 3 cut(s) 696, 1611, 2742
BciVI GTATCC 1 cut(s) 2225
BclI TGATCA 1 cut(s) 1621
BcoDI GTCTC 8 cut(s) 9, 446, 523, 599, 1527, 2167, 2331, 2709
BfaI CTAG 9 cut(s) 230, 723, 1319, 1629, 1635, 1895, 2415, 2451, 2796
BfoI RGCGCY 1 cut(s) 974
BfrI CTTAAG 1 cut(s) 1964
BfuAI ACCTGC 2 cut(s) 664, 863
BfuI GTATCC 1 cut(s) 2225
BisI GCNGC 9 cut(s) 974, 1014, 1022, 1062, 1095, 1269, 1831, 1844, 1949
BlpI GCTNAGC 2 cut(s) 285, 2006
BlsI GCNGC 9 cut(s) 975, 1015, 1023, 1063, 1096, 1270, 1832, 1845, 1950
Bme1390I CCNGG 3 cut(s) 696, 1611, 2742
Bme18I GGWCC 4 cut(s) 509, 698, 1533, 1898
BmgT120I GGNCC 5 cut(s) 509, 698, 1165, 1533, 1898
BmiI GGNNCC 9 cut(s) 345, 511, 678, 1098, 1167, 1535, 1683, 2365, 2497
BmrFI CCNGG 3 cut(s) 696, 1611, 2742
BmsI GCATC 4 cut(s) 842, 1189, 1792, 2290
BplI GAGNNNNNCTC 2 cut(s) 2167, 2199
BpmI CTGGAG 1 cut(s) 1023
Bpu1102I GCTNAGC 2 cut(s) 285, 2006
BpuEI CTTGAG 2 cut(s) 813, 2093
Bsa29I ATCGAT 1 cut(s) 761
BsaAI YACGTR 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 2167
BsaJI CCNNGG 4 cut(s) 123, 1425, 1609, 1867
BsaWI WCCGGW 2 cut(s) 1144, 2563
Bsc4I CCNNNNNNNGG 4 cut(s) 506, 608, 1200, 1201
Bse118I RCCGGY 3 cut(s) 1144, 1162, 2745
Bse1I ACTGG 5 cut(s) 410, 534, 613, 1475, 2276
Bse3DI GCAATG 3 cut(s) 1840, 2139, 2199
BseBI CCWGG 3 cut(s) 696, 1611, 2742
BseCI ATCGAT 1 cut(s) 761
BseDI CCNNGG 4 cut(s) 123, 1425, 1609, 1867
BseGI GGATG 6 cut(s) 1069, 1079, 1180, 1210, 1594, 2770
BseLI CCNNNNNNNGG 4 cut(s) 506, 608, 1200, 1201
BseMI GCAATG 3 cut(s) 1840, 2139, 2199
BseMII CTCAG 8 cut(s) 276, 393, 534, 1262, 1575, 1751, 2412, 2551
BseNI ACTGG 5 cut(s) 410, 534, 613, 1475, 2276
BseRI GAGGAG 7 cut(s) 320, 1023, 1026, 1098, 1146, 1295, 2253
BseSI GKGCMC 1 cut(s) 1228
BseXI GCAGC 6 cut(s) 1025, 1073, 1255, 1830, 1842, 1935
Bsh1236I CGCG 3 cut(s) 1001, 1006, 1049
BshFI GGCC 6 cut(s) 279, 995, 1166, 1633, 1779, 2745
BshNI GGYRCC 4 cut(s) 676, 1096, 1681, 2495
BshTI ACCGGT 1 cut(s) 1144
BshVI ATCGAT 1 cut(s) 761
BsiHKAI GWGCWC 4 cut(s) 1228, 1712, 2440, 2598
BsiSI CCGG 5 cut(s) 992, 1145, 1163, 2564, 2746
BslFI GGGAC 5 cut(s) 495, 737, 753, 1036, 1519
BslI CCNNNNNNNGG 4 cut(s) 506, 608, 1200, 1201
BsmAI GTCTC 8 cut(s) 9, 446, 523, 599, 1527, 2167, 2331, 2709
BsmBI CGTCTC 1 cut(s) 2331
BsmFI GGGAC 5 cut(s) 495, 737, 753, 1036, 1519
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 6 cut(s) 279, 995, 1166, 1633, 1779, 2745
Bso31I GGTCTC 1 cut(s) 2167
Bsp1286I GDGCHC 5 cut(s) 1228, 1712, 2237, 2440, 2598
Bsp1720I GCTNAGC 2 cut(s) 285, 2006
BspANI GGCC 6 cut(s) 279, 995, 1166, 1633, 1779, 2745
BspCNI CTCAG 8 cut(s) 277, 392, 533, 1261, 1576, 1750, 2413, 2550
BspDI ATCGAT 1 cut(s) 761
BspFNI CGCG 3 cut(s) 1001, 1006, 1049
BspHI TCATGA 2 cut(s) 132, 1975
BspLI GGNNCC 9 cut(s) 345, 511, 678, 1098, 1167, 1535, 1683, 2365, 2497
BspMI ACCTGC 2 cut(s) 664, 863
BspPI GGATC 5 cut(s) 776, 1133, 1399, 1899, 2497
BspT107I GGYRCC 4 cut(s) 676, 1096, 1681, 2495
BspTI CTTAAG 1 cut(s) 1964
BspTNI GGTCTC 1 cut(s) 2167
BsrBI CCGCTC 3 cut(s) 827, 976, 1402
BsrDI GCAATG 3 cut(s) 1840, 2139, 2199
BsrFI RCCGGY 3 cut(s) 1144, 1162, 2745
BsrI ACTGG 5 cut(s) 410, 534, 613, 1475, 2276
BssAI RCCGGY 3 cut(s) 1144, 1162, 2745
BssECI CCNNGG 4 cut(s) 123, 1425, 1609, 1867
BssSI CACGAG 4 cut(s) 1524, 1686, 2184, 2628
BssT1I CCWWGG 3 cut(s) 123, 1425, 1867
Bst2BI CACGAG 4 cut(s) 1524, 1686, 2184, 2628
Bst2UI CCWGG 3 cut(s) 696, 1611, 2742
Bst4CI ACNGT 8 cut(s) 155, 751, 861, 886, 1348, 1460, 1504, 2404
Bst6I CTCTTC 2 cut(s) 330, 477
BstAFI CTTAAG 1 cut(s) 1964
BstAPI GCANNNNNTGC 1 cut(s) 1688
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 8 cut(s) 963, 1092, 1266, 1488, 1781, 2012, 2578, 2673
BstDEI CTNAG 9 cut(s) 285, 379, 520, 1248, 1584, 1737, 2006, 2421, 2537
BstEII GGTNACC 1 cut(s) 1189
BstF5I GGATG 6 cut(s) 1069, 1079, 1180, 1210, 1594, 2770
BstFNI CGCG 3 cut(s) 1001, 1006, 1049
BstH2I RGCGCY 1 cut(s) 974
BstHHI GCGC 5 cut(s) 90, 291, 961, 973, 1008
BstMAI GTCTC 8 cut(s) 9, 446, 523, 599, 1527, 2167, 2331, 2709
BstMWI GCNNNNNNNGC 4 cut(s) 1688, 2011, 2309, 2593
BstNI CCWGG 3 cut(s) 696, 1611, 2742
BstPI GGTNACC 1 cut(s) 1189
BstSCI CCNGG 3 cut(s) 694, 1609, 2740
BstSLI GKGCMC 1 cut(s) 1228
BstUI CGCG 3 cut(s) 1001, 1006, 1049
BstV1I GCAGC 6 cut(s) 1025, 1073, 1255, 1830, 1842, 1935
BstX2I RGATCY 3 cut(s) 781, 1391, 1891
BstXI CCANNNNNNTGG 1 cut(s) 2551
BstYI RGATCY 3 cut(s) 781, 1391, 1891
Bsu15I ATCGAT 1 cut(s) 761
BsuI GTATCC 1 cut(s) 2225
BsuRI GGCC 6 cut(s) 279, 995, 1166, 1633, 1779, 2745
BsuTUI ATCGAT 1 cut(s) 761
BtgZI GCGATG 1 cut(s) 1161
BtsCI GGATG 6 cut(s) 1069, 1079, 1180, 1210, 1594, 2770
BtsI GCAGTG 2 cut(s) 1023, 1085
BtsIMutI CAGTG 4 cut(s) 857, 1023, 1085, 1353
BveI ACCTGC 2 cut(s) 664, 863
Cac8I GCNNGC 8 cut(s) 963, 1092, 1266, 1488, 1781, 2012, 2578, 2673
CciI TCATGA 2 cut(s) 132, 1975
CfoI GCGC 5 cut(s) 90, 291, 961, 973, 1008
Cfr10I RCCGGY 3 cut(s) 1144, 1162, 2745
Cfr13I GGNCC 5 cut(s) 509, 698, 1165, 1533, 1898
ClaI ATCGAT 1 cut(s) 761
CseI GACGC 4 cut(s) 459, 990, 1038, 1067
Csp6I GTAC 5 cut(s) 677, 1239, 1405, 1500, 2022
CspAI ACCGGT 1 cut(s) 1144
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviQI GTAC 5 cut(s) 677, 1239, 1405, 1500, 2022
DdeI CTNAG 9 cut(s) 285, 379, 520, 1248, 1584, 1737, 2006, 2421, 2537
DraI TTTAAA 1 cut(s) 664
DraIII CACNNNGTG 2 cut(s) 1688, 2611
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 2 cut(s) 993, 1777
Eam1104I CTCTTC 2 cut(s) 330, 477
EarI CTCTTC 2 cut(s) 330, 477
EciI GGCGGA 1 cut(s) 901
Ecl136II GAGCTC 2 cut(s) 2438, 2596
Eco130I CCWWGG 3 cut(s) 123, 1425, 1867
Eco147I AGGCCT 1 cut(s) 1633
Eco24I GRGCYC 3 cut(s) 2237, 2440, 2598
Eco31I GGTCTC 1 cut(s) 2167
Eco47I GGWCC 4 cut(s) 509, 698, 1533, 1898
Eco53kI GAGCTC 2 cut(s) 2438, 2596
Eco57I CTGAAG 1 cut(s) 762
Eco91I GGTNACC 1 cut(s) 1189
EcoICRI GAGCTC 2 cut(s) 2438, 2596
EcoO109I RGGNCCY 1 cut(s) 1898
EcoO65I GGTNACC 1 cut(s) 1189
EcoRII CCWGG 3 cut(s) 694, 1609, 2740
EcoT14I CCWWGG 3 cut(s) 123, 1425, 1867
EcoT38I GRGCYC 3 cut(s) 2237, 2440, 2598
ErhI CCWWGG 3 cut(s) 123, 1425, 1867
Esp3I CGTCTC 1 cut(s) 2331
FaqI GGGAC 5 cut(s) 495, 737, 753, 1036, 1519
FauI CCCGC 4 cut(s) 877, 947, 1177, 2585
FbaI TGATCA 1 cut(s) 1621
FblI GTMKAC 1 cut(s) 2336
Fnu4HI GCNGC 9 cut(s) 974, 1014, 1022, 1062, 1095, 1269, 1831, 1844, 1949
FokI GGATG 6 cut(s) 1056, 1066, 1167, 1217, 1601, 2777
FriOI GRGCYC 3 cut(s) 2237, 2440, 2598
Fsp4HI GCNGC 9 cut(s) 974, 1014, 1022, 1062, 1095, 1269, 1831, 1844, 1949
FspBI CTAG 9 cut(s) 230, 723, 1319, 1629, 1635, 1895, 2415, 2451, 2796
GlaI GCGC 5 cut(s) 89, 290, 960, 972, 1007
GluI GCNGC 9 cut(s) 974, 1014, 1022, 1062, 1095, 1269, 1831, 1844, 1949
GsuI CTGGAG 1 cut(s) 1023
HaeII RGCGCY 1 cut(s) 974
HaeIII GGCC 6 cut(s) 279, 995, 1166, 1633, 1779, 2745
HapII CCGG 5 cut(s) 992, 1145, 1163, 2564, 2746
HgaI GACGC 4 cut(s) 459, 990, 1038, 1067
HhaI GCGC 5 cut(s) 90, 291, 961, 973, 1008
Hin6I GCGC 5 cut(s) 88, 289, 959, 971, 1006
HinP1I GCGC 5 cut(s) 88, 289, 959, 971, 1006
HincII GTYRAC 1 cut(s) 2155
HindII GTYRAC 1 cut(s) 2155
HinfI GANTC 7 cut(s) 878, 1315, 1741, 1751, 2077, 2176, 2423
HpaI GTTAAC 1 cut(s) 2155
HpaII CCGG 5 cut(s) 992, 1145, 1163, 2564, 2746
HphI GGTGA 8 cut(s) 887, 894, 1113, 1183, 1384, 1472, 2119, 2713
Hpy166II GTNNAC 6 cut(s) 701, 714, 1226, 2155, 2259, 2337
Hpy188III TCNNGA 8 cut(s) 133, 1136, 1202, 1976, 2093, 2135, 2661, 2706
Hpy8I GTNNAC 6 cut(s) 701, 714, 1226, 2155, 2259, 2337
Hpy99I CGWCG 4 cut(s) 948, 1006, 1061, 2334
HpyCH4III ACNGT 8 cut(s) 155, 751, 861, 886, 1348, 1460, 1504, 2404
HpyCH4IV ACGT 8 cut(s) 195, 244, 628, 633, 2212, 2226, 2324, 2410
HpyF10VI GCNNNNNNNGC 4 cut(s) 1688, 2011, 2309, 2593
HpyF3I CTNAG 9 cut(s) 285, 379, 520, 1248, 1584, 1737, 2006, 2421, 2537
HpySE526I ACGT 8 cut(s) 195, 244, 628, 633, 2212, 2226, 2324, 2410
HspAI GCGC 5 cut(s) 88, 289, 959, 971, 1006
KpnI GGTACC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 1621
KspAI GTTAAC 1 cut(s) 2155
LmnI GCTCC 3 cut(s) 818, 1132, 1159
Lsp1109I GCAGC 6 cut(s) 1025, 1073, 1255, 1830, 1842, 1935
LweI GCATC 4 cut(s) 842, 1189, 1792, 2290
MaeI CTAG 9 cut(s) 230, 723, 1319, 1629, 1635, 1895, 2415, 2451, 2796
MaeII ACGT 8 cut(s) 195, 244, 628, 633, 2212, 2226, 2324, 2410
MbiI CCGCTC 3 cut(s) 827, 976, 1402
MflI RGATCY 3 cut(s) 781, 1391, 1891
MhlI GDGCHC 5 cut(s) 1228, 1712, 2237, 2440, 2598
MlsI TGGCCA 1 cut(s) 1779
MluCI AATT 7 cut(s) 164, 864, 1117, 1544, 1649, 2168, 2514
MluNI TGGCCA 1 cut(s) 1779
MlyI GAGTC 5 cut(s) 872, 1309, 1750, 2185, 2432
MmeI TCCRAC 3 cut(s) 265, 873, 2345
Mox20I TGGCCA 1 cut(s) 1779
MscI TGGCCA 1 cut(s) 1779
MslI CAYNNNNRTG 4 cut(s) 838, 1941, 2451, 2549
Msp20I TGGCCA 1 cut(s) 1779
MspA1I CMGCKG 2 cut(s) 1843, 2675
MspCI CTTAAG 1 cut(s) 1964
MspI CCGG 5 cut(s) 992, 1145, 1163, 2564, 2746
MspR9I CCNGG 3 cut(s) 696, 1611, 2742
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 3 cut(s) 696, 1611, 2742
MvnI CGCG 3 cut(s) 1001, 1006, 1049
MwoI GCNNNNNNNGC 4 cut(s) 1688, 2011, 2309, 2593
NlaIV GGNNCC 9 cut(s) 345, 511, 678, 1098, 1167, 1535, 1683, 2365, 2497
NmuCI GTSAC 6 cut(s) 196, 624, 875, 886, 1189, 2636
PagI TCATGA 2 cut(s) 132, 1975
PaqCI CACCTGC 1 cut(s) 863
PceI AGGCCT 1 cut(s) 1633
PcsI WCGNNNNNNNCGW 1 cut(s) 2218
PctI GAATGC 1 cut(s) 234
PfeI GAWTC 2 cut(s) 1751, 2077
PflMI CCANNNNNTGG 1 cut(s) 608
PinAI ACCGGT 1 cut(s) 1144
PkrI GCNGC 9 cut(s) 975, 1015, 1023, 1063, 1096, 1270, 1832, 1845, 1950
PleI GAGTC 5 cut(s) 872, 1309, 1749, 2184, 2431
PpsI GAGTC 5 cut(s) 872, 1309, 1749, 2184, 2431
Ppu21I YACGTR 1 cut(s) 629
PpuMI RGGWCCY 1 cut(s) 1898
PshBI ATTAAT 1 cut(s) 1956
Psp124BI GAGCTC 2 cut(s) 2440, 2598
Psp1406I AACGTT 2 cut(s) 244, 633
Psp5II RGGWCCY 1 cut(s) 1898
Psp6I CCWGG 3 cut(s) 694, 1609, 2740
PspEI GGTNACC 1 cut(s) 1189
PspGI CCWGG 3 cut(s) 694, 1609, 2740
PspN4I GGNNCC 9 cut(s) 345, 511, 678, 1098, 1167, 1535, 1683, 2365, 2497
PspPI GGNCC 5 cut(s) 509, 698, 1165, 1533, 1898
PspPPI RGGWCCY 1 cut(s) 1898
PsuI RGATCY 3 cut(s) 781, 1391, 1891
PvuII CAGCTG 2 cut(s) 1843, 2675
RsaI GTAC 5 cut(s) 678, 1240, 1406, 1501, 2023
RsaNI GTAC 5 cut(s) 677, 1239, 1405, 1500, 2022
RseI CAYNNNNRTG 4 cut(s) 838, 1941, 2451, 2549
SacI GAGCTC 2 cut(s) 2440, 2598
SatI GCNGC 9 cut(s) 974, 1014, 1022, 1062, 1095, 1269, 1831, 1844, 1949
Sau96I GGNCC 5 cut(s) 509, 698, 1165, 1533, 1898
SchI GAGTC 5 cut(s) 872, 1309, 1750, 2185, 2432
ScrFI CCNGG 3 cut(s) 696, 1611, 2742
SduI GDGCHC 5 cut(s) 1228, 1712, 2237, 2440, 2598
SfaNI GCATC 4 cut(s) 842, 1189, 1792, 2290
SinI GGWCC 4 cut(s) 509, 698, 1533, 1898
SmiMI CAYNNNNRTG 4 cut(s) 838, 1941, 2451, 2549
SmlI CTYRAG 3 cut(s) 828, 1964, 2072
SmoI CTYRAG 3 cut(s) 828, 1964, 2072
Sse9I AATT 7 cut(s) 164, 864, 1117, 1544, 1649, 2168, 2514
SseBI AGGCCT 1 cut(s) 1633
SspMI CTAG 9 cut(s) 230, 723, 1319, 1629, 1635, 1895, 2415, 2451, 2796
SstI GAGCTC 2 cut(s) 2440, 2598
StuI AGGCCT 1 cut(s) 1633
StyD4I CCNGG 3 cut(s) 694, 1609, 2740
StyI CCWWGG 3 cut(s) 123, 1425, 1867
TaaI ACNGT 8 cut(s) 155, 751, 861, 886, 1348, 1460, 1504, 2404
TaiI ACGT 8 cut(s) 198, 247, 631, 636, 2215, 2229, 2327, 2413
TaqI TCGA 6 cut(s) 31, 417, 761, 1511, 2598, 2707
TaqII GACCGA 1 cut(s) 2322
TasI AATT 7 cut(s) 164, 864, 1117, 1544, 1649, 2168, 2514
TauI GCSGC 3 cut(s) 976, 1024, 1097
TfiI GAWTC 2 cut(s) 1751, 2077
TscAI CASTG 4 cut(s) 864, 1023, 1092, 1353
TseFI GTSAC 6 cut(s) 196, 624, 875, 886, 1189, 2636
TseI GCWGC 6 cut(s) 1013, 1061, 1268, 1830, 1843, 1948
Tsp45I GTSAC 6 cut(s) 196, 624, 875, 886, 1189, 2636
TspDTI ATGAA 5 cut(s) 660, 1559, 2270, 2513, 2641
TspGWI ACGGA 3 cut(s) 203, 1404, 2726
TspRI CASTG 4 cut(s) 864, 1023, 1092, 1353
Van91I CCANNNNNTGG 1 cut(s) 608
Vha464I CTTAAG 1 cut(s) 1964
VneI GTGCAC 1 cut(s) 1224
VpaK11BI GGWCC 4 cut(s) 509, 698, 1533, 1898
VspI ATTAAT 1 cut(s) 1956
XapI RAATTY 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 2336
XspI CTAG 9 cut(s) 230, 723, 1319, 1629, 1635, 1895, 2415, 2451, 2796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.