RchiOBHm_Chr6g0261771
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003077
Physical Location & Seq
Forward (+)
16589970 .. 16591234
1265 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23475

Sequence Viewer

Length: 741 bp
ATGAAACTTGTTAGAGCAAATCACTCTCCTACATTGATTTCCTCCCTCTCCACAATCAAAACTGATTGTCATATTGTGCGTTGCAAATGGGTGTTCCGCATTAAGCGGAACCCGGATGGCTCAGTTGAGCGTTACAAGGCCCGCCTTGTTGCCAAAGGATTCCATCAACAGCACGGGATCGACTATGGTGAAACCTATAGTCCTGTTATCAAGCCCGCTACCATTCGAACCGTTCACTGTGTTGCTGTGTCTAGGTGTTGGCCTCTCCGTCAGCTAGATGTCAAAAATGCATTCCTCCATGGCTATCTTCAGGAACACGTTTACATGACTCAGCCTCCTGGCTTCGTCAATTCCTCTCGGCCCTCCTATGTCTGTAAGCTTCACAAGGCACTTTATGGCCTCAAACAAGCACCCCGTGCTTGGTTTCAACGCATGAGCTCTTTTCTGCTTTCTGTTGGCTTTCGACATAGTAAAGCTGACTCATCTTTATTCATCTATCAACATGGACTGCATACAATTTTCTTTTTACTCTATGTTGATGACATTGTCATTACGGGCAGTAGTGATCAGCTTCTTCAAAGTTTTATTGATGCCTTGGGCCGTGGATTTGACATTAAGGATCTTGGTCCCCTTCACTATTTCCTTGGTTTTATTGATGCCTTGGGCCGTGGATTTGACATTAAGGATCTCATTCCAACGTCCTTCACATCAATCAACTCAAGTATGCTCATGATATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

246

Amino Acids

28.03

Weight (kDa)

9.61

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 23 - 217 1.3e-57 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 97, 106, 142, 216
AclWI GGATC 3 cut(s) 185, 627, 693
AcuI CTGAAG 1 cut(s) 293
AdeI CACNNNGTG 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 420
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 2 cut(s) 428, 578
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 5 cut(s) 274, 379, 438, 476, 571
AluI AGCT 5 cut(s) 274, 379, 438, 476, 571
Alw21I GWGCWC 1 cut(s) 440
AlwI GGATC 3 cut(s) 185, 627, 693
AoxI GGCC 6 cut(s) 138, 260, 359, 397, 598, 664
AspS9I GGNCC 5 cut(s) 139, 360, 598, 626, 664
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 1 cut(s) 200
AsuII TTCGAA 1 cut(s) 226
AvaII GGWCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 440
BccI CCATC 2 cut(s) 110, 171
BceAI ACGGC 2 cut(s) 585, 651
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 1 cut(s) 113
BfaI CTAG 2 cut(s) 252, 275
BfmI CTRYAG 1 cut(s) 196
Bme1390I CCNGG 2 cut(s) 113, 339
Bme18I GGWCC 1 cut(s) 626
BmgT120I GGNCC 5 cut(s) 139, 360, 598, 626, 664
BmiI GGNNCC 2 cut(s) 110, 628
BmrFI CCNGG 2 cut(s) 113, 339
BmsI GCATC 2 cut(s) 580, 646
Bpu14I TTCGAA 1 cut(s) 226
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 1 cut(s) 113
BsaJI CCNNGG 6 cut(s) 298, 594, 601, 643, 660, 667
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 6 cut(s) 298, 594, 601, 643, 660, 667
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 135, 344
BshFI GGCC 6 cut(s) 140, 262, 361, 399, 600, 666
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 1 cut(s) 612
BslI CCNNNNNNNGG 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 612
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 6 cut(s) 140, 262, 361, 399, 600, 666
Bsp119I TTCGAA 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 4 cut(s) 177, 565, 619, 685
Bsp19I CCATGG 1 cut(s) 298
BspACI CCGC 4 cut(s) 97, 106, 142, 216
BspANI GGCC 6 cut(s) 140, 262, 361, 399, 600, 666
BspCNI CTCAG 2 cut(s) 134, 343
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 2 cut(s) 110, 628
BspPI GGATC 3 cut(s) 185, 627, 693
BspT104I TTCGAA 1 cut(s) 226
BssECI CCNNGG 6 cut(s) 298, 594, 601, 643, 660, 667
BssMI GATC 4 cut(s) 177, 565, 619, 685
BssT1I CCWWGG 4 cut(s) 298, 594, 643, 660
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 2 cut(s) 232, 239
BstAPI GCANNNNNTGC 1 cut(s) 416
BstBI TTCGAA 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 142, 216
BstDEI CTNAG 2 cut(s) 121, 330
BstDSI CCRYGG 3 cut(s) 298, 601, 667
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 4 cut(s) 180, 568, 622, 688
BstMBI GATC 4 cut(s) 177, 565, 619, 685
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 111, 337
BstSFI CTRYAG 1 cut(s) 196
BstX2I RGATCY 2 cut(s) 619, 685
BstYI RGATCY 2 cut(s) 619, 685
BsuRI GGCC 6 cut(s) 140, 262, 361, 399, 600, 666
BtgI CCRYGG 3 cut(s) 298, 601, 667
BtsCI GGATG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 142, 216
CciI TCATGA 1 cut(s) 729
Cfr13I GGNCC 5 cut(s) 139, 360, 598, 626, 664
CviAII CATG 5 cut(s) 299, 325, 433, 503, 730
DdeI CTNAG 2 cut(s) 121, 330
DpnI GATC 4 cut(s) 179, 567, 621, 687
DpnII GATC 4 cut(s) 177, 565, 619, 685
DraIII CACNNNGTG 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 438
Eco130I CCWWGG 4 cut(s) 298, 594, 643, 660
Eco24I GRGCYC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 4 cut(s) 298, 594, 643, 660
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 4 cut(s) 298, 594, 643, 660
FaeI CATG 5 cut(s) 302, 328, 436, 506, 733
FaqI GGGAC 1 cut(s) 612
FatI CATG 5 cut(s) 298, 324, 432, 502, 729
FauI CCCGC 2 cut(s) 149, 223
FbaI TGATCA 1 cut(s) 565
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 440
FspBI CTAG 2 cut(s) 252, 275
HaeIII GGCC 6 cut(s) 140, 262, 361, 399, 600, 666
HapII CCGG 1 cut(s) 113
Hin1II CATG 5 cut(s) 302, 328, 436, 506, 733
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 3 cut(s) 159, 328, 479
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 235, 322
Hpy188III TCNNGA 2 cut(s) 311, 730
Hpy8I GTNNAC 2 cut(s) 235, 322
HpyAV CCTTC 2 cut(s) 641, 712
HpyCH4III ACNGT 2 cut(s) 232, 239
HpyCH4IV ACGT 2 cut(s) 318, 698
HpyCH4V TGCA 3 cut(s) 84, 290, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
HpyF3I CTNAG 2 cut(s) 121, 330
HpySE526I ACGT 2 cut(s) 318, 698
Hsp92II CATG 5 cut(s) 302, 328, 436, 506, 733
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 4 cut(s) 177, 565, 619, 685
LpnPI CCDG 5 cut(s) 126, 216, 296, 324, 351
LweI GCATC 2 cut(s) 580, 646
MaeI CTAG 2 cut(s) 252, 275
MaeII ACGT 2 cut(s) 318, 698
MaeIII GTNAC 1 cut(s) 131
MalI GATC 4 cut(s) 179, 567, 621, 687
MboI GATC 4 cut(s) 177, 565, 619, 685
MboII GAAGA 2 cut(s) 299, 566
MflI RGATCY 2 cut(s) 619, 685
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 2 cut(s) 349, 516
MlyI GAGTC 2 cut(s) 322, 473
MmeI TCCRAC 1 cut(s) 719
MnlI CCTC 8 cut(s) 52, 56, 273, 305, 345, 364, 373, 410
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 4 cut(s) 102, 615, 681, 739
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 2 cut(s) 113, 339
Mva1269I GAATGC 1 cut(s) 290
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 416
NciI CCSGG 1 cut(s) 113
NcoI CCATGG 1 cut(s) 298
NdeII GATC 4 cut(s) 177, 565, 619, 685
NlaIII CATG 5 cut(s) 302, 328, 436, 506, 733
NlaIV GGNNCC 2 cut(s) 110, 628
NmeAIII GCCGAG 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 292
NspV TTCGAA 1 cut(s) 226
PagI TCATGA 1 cut(s) 729
PctI GAATGC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 159
PflFI GACNNNGTC 1 cut(s) 545
PleI GAGTC 2 cut(s) 322, 473
PpsI GAGTC 2 cut(s) 322, 473
Psp124BI GAGCTC 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 110, 628
PspPI GGNCC 5 cut(s) 139, 360, 598, 626, 664
PsuI RGATCY 2 cut(s) 619, 685
PsyI GACNNNGTC 1 cut(s) 545
SacI GAGCTC 1 cut(s) 440
SaqAI TTAA 4 cut(s) 102, 615, 681, 739
Sau3AI GATC 4 cut(s) 177, 565, 619, 685
Sau96I GGNCC 5 cut(s) 139, 360, 598, 626, 664
SchI GAGTC 2 cut(s) 322, 473
ScrFI CCNGG 2 cut(s) 113, 339
SduI GDGCHC 1 cut(s) 440
SetI ASST 9 cut(s) 197, 257, 276, 321, 381, 440, 478, 573, 701
SfaNI GCATC 2 cut(s) 580, 646
SfcI CTRYAG 1 cut(s) 196
SfuI TTCGAA 1 cut(s) 226
SinI GGWCC 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
Sse9I AATT 2 cut(s) 349, 516
SsiI CCGC 4 cut(s) 97, 106, 142, 216
SspMI CTAG 2 cut(s) 252, 275
SstI GAGCTC 1 cut(s) 440
StyD4I CCNGG 2 cut(s) 111, 337
StyI CCWWGG 4 cut(s) 298, 594, 643, 660
TaaI ACNGT 2 cut(s) 232, 239
TaiI ACGT 2 cut(s) 321, 701
TaqI TCGA 3 cut(s) 180, 226, 463
TasI AATT 2 cut(s) 349, 516
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 4 cut(s) 102, 615, 681, 739
Tru9I TTAA 4 cut(s) 102, 615, 681, 739
TscAI CASTG 1 cut(s) 242
TspDTI ATGAA 2 cut(s) 17, 481
TspGWI ACGGA 1 cut(s) 257
TspRI CASTG 1 cut(s) 242
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 626
XspI CTAG 2 cut(s) 252, 275
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.