FvH4_3g09472

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001466
Physical Location & Seq
Reverse (-)
5518495 .. 5520430
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09472.t1

Sequence Viewer

Length: 1629 bp
ATGCACATCGTAGCCTTTGCACAGTATCATGCTGGTCACTTTCGATCTGTTCAAATGGGTAGCCTTCCATTCCTCCATGATCCAATAGAAAACAGCTCATCCCACCTGACCAAACCTCTAGACTCTGAAGAGTTCAGGAAACAAGGGTATATGGTCATAGACTTCATTGCAGATTACTACCAAAACATAGAGAACTACCCAGTTTTAAGCCGAGTAGAATCCGGATATTTGAAAAAATGCCTACCTGATTCTGCCCCATATAACCCTGATTCAATACAAACAATACTCCAAGACGTTAAAGATCACATTATTCCAGGCCTAACACATTGGCAAAGTCCTAACTTTTTTGCATACTTTTCTTCGACGGCAAGCACTGCTGGGTTTCTTGGTGAAATGCTCACAACTGGATTCAATGTAGTGGGGTTCAATTGGGTTTCATCGCCGGCTGCAACTGAACTCGAACACTTGGTCATGGAATGGCTTGCAGACATGCTTCAGCTTCCCAAGTCGTTTCATTTCTCTGGTAATGGTGGTGGTGTGTTACATGGGAGTACTTGCGAAGCCATTGTTTGCACAATGGTAGCTGCCAGGGACCAAATGCTAAGCCAAATTGGTAGCGACAATCTGGGAAAACTTGTTGTGTATGGTTCAGACCAAACACACAGCACACTTCAAAAAGCTACCCAAATTGTTGGAATCAAACCAGAGAACTTCAGGGTCATCAAGACTACAAAGTCAGCTGAATTTGCTCTATCACCTGAAATGCTAAGATTGACAGTTTGTTCAGACTTGGACAAAGGGCTGCTGCCACTTTTCCTATGTGCCACAATAGGCACAACCTCAACCACTGCTATCGATCCATTGGAGGCACTCTGTGATGTAGCAAAAGAACACGGCATTTGGGTTCATGTGGATGCTGCATATGCAGGAAGTGCTTGCATTTGTCCAGAGTTTCGTCATTTCATTGATGGCGTTGAGGGTGCAAACTCCTTCAGTTTCAACCCACATAAATGGTTATTTACTGCGATGGACTGTTGCTGCCTTTGGGTTAAGAACCCAAGTGCATTGATACGTTCACTGTCGACGAATCCGGAGTATTTGAGGAACAAAGCCAGTGATTCAAAGCAGGTGGTGGACTACAAAGACTGGCAAATAGCACTAAGCCGGAGGTTCCGGGCATTGAAGATGTGGCTTTTGCTAAGAAGCTATGGCGTAGCTAACCTTAGAAGCTTTATTCGAAGCCATGTCAAGATGGCCAAGATTTTTGAAGTGCTGGTGAGAATGGACAACAGGTTTGAGATTGTGGTGCCTAGAAACTTCTCCTTGGTCTGTTTCAGGATTTCACCATCCGCAATAGTTAGTGATGATCATTCTTATAATGGTGAGGAGTACAATATGGTTAACGAGATCAACTGCAAATTGCTGGAGGCCATCAATGCATCAGGCTCTGTGTTCATGACTCATGCTGTGGTTGGAGGTGTCTACCTGCTGCGGTGCGCAGTTGGTGCAACTCTAACAGAGGAAAAACACATTGTCGAGGCATGGAAGGTGGTGCAAGGTCAGGCACATGCAATTCTCTCAGCATGCATAGTTGATCTGCATAATCCTATGAGATATCCTAACATATAG

Protein Analysis

543

Amino Acids

60.41

Weight (kDa)

6.23

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 75 - 447 4.6e-115 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1377
AarI CACCTGC 1 cut(s) 1117
AasI GACNNNNNNGTC 1 cut(s) 733
Acc16I TGCGCA 1 cut(s) 1498
Acc36I ACCTGC 2 cut(s) 1117, 1494
AccB1I GGYRCC 1 cut(s) 1306
AccI GTMKAC 2 cut(s) 1082, 1482
AccIII TCCGGA 2 cut(s) 221, 1090
AciI CCGC 2 cut(s) 1350, 1492
AclWI GGATC 2 cut(s) 74, 851
AcoI YGGCCR 1 cut(s) 1254
AcsI RAATTY 1 cut(s) 743
AcuI CTGAAG 4 cut(s) 147, 479, 697, 976
AdeI CACNNNGTG 1 cut(s) 875
AfaI GTAC 2 cut(s) 553, 1391
AjnI CCWGG 2 cut(s) 313, 587
AluBI AGCT 8 cut(s) 96, 499, 584, 680, 740, 1206, 1217, 1230
AluI AGCT 8 cut(s) 96, 499, 584, 680, 740, 1206, 1217, 1230
AlwI GGATC 2 cut(s) 74, 851
AlwNI CAGNNNCTG 1 cut(s) 1448
Aor13HI TCCGGA 2 cut(s) 221, 1090
AoxI GGCC 3 cut(s) 316, 1254, 1428
ApeKI GCWGC 7 cut(s) 446, 584, 802, 805, 917, 1038, 1489
ApoI RAATTY 1 cut(s) 743
ArsI GACNNNNNNTTYG 4 cut(s) 453, 485, 1277, 1309
AspLEI GCGC 1 cut(s) 1499
AspS9I GGNCC 1 cut(s) 592
AsuC2I CCSGG 1 cut(s) 1175
AsuHPI GGTGA 5 cut(s) 401, 747, 1288, 1335, 1394
AsuII TTCGAA 1 cut(s) 1237
AvaII GGWCC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 1256
BanI GGYRCC 1 cut(s) 1306
BbvI GCAGC 7 cut(s) 433, 571, 789, 792, 904, 1025, 1476
BccI CCATC 5 cut(s) 962, 1021, 1246, 1354, 1439
BceAI ACGGC 2 cut(s) 381, 910
BciT130I CCWGG 2 cut(s) 315, 589
BclI TGATCA 1 cut(s) 1366
BcnI CCSGG 1 cut(s) 1175
BfaI CTAG 2 cut(s) 119, 1311
BfuAI ACCTGC 2 cut(s) 1117, 1494
BisI GCNGC 7 cut(s) 447, 585, 803, 806, 918, 1039, 1490
BlpI GCTNAGC 1 cut(s) 602
BlsI GCNGC 7 cut(s) 448, 586, 804, 807, 919, 1040, 1491
BmcAI AGTACT 1 cut(s) 553
Bme1390I CCNGG 3 cut(s) 315, 589, 1175
Bme18I GGWCC 1 cut(s) 592
BmgT120I GGNCC 1 cut(s) 592
BmiI GGNNCC 3 cut(s) 593, 1172, 1308
BmrFI CCNGG 3 cut(s) 315, 589, 1175
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 904, 1448
BmuI ACTGGG 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 1445
Bpu1102I GCTNAGC 1 cut(s) 602
Bpu14I TTCGAA 1 cut(s) 1237
BpuMI CCSGG 1 cut(s) 1175
Bsa29I ATCGAT 1 cut(s) 855
BsaJI CCNNGG 2 cut(s) 588, 1323
BsaWI WCCGGW 2 cut(s) 221, 1090
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 4 cut(s) 200, 409, 1113, 1151
Bse3DI GCAATG 1 cut(s) 165
BseAI TCCGGA 2 cut(s) 221, 1090
BseBI CCWGG 2 cut(s) 315, 589
BseCI ATCGAT 1 cut(s) 855
BseDI CCNNGG 2 cut(s) 588, 1323
BseGI GGATG 3 cut(s) 98, 919, 1346
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 1593
BseNI ACTGG 4 cut(s) 200, 409, 1113, 1151
BseRI GAGGAG 1 cut(s) 1400
BseXI GCAGC 7 cut(s) 433, 571, 789, 792, 904, 1025, 1476
BseYI CCCAGC 1 cut(s) 377
BshFI GGCC 3 cut(s) 318, 1256, 1430
BshNI GGYRCC 1 cut(s) 1306
BshVI ATCGAT 1 cut(s) 855
BsiSI CCGG 5 cut(s) 222, 443, 1091, 1165, 1174
BslFI GGGAC 1 cut(s) 605
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 3 cut(s) 318, 1256, 1430
Bsp119I TTCGAA 1 cut(s) 1237
Bsp13I TCCGGA 2 cut(s) 221, 1090
Bsp143I GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
Bsp1720I GCTNAGC 1 cut(s) 602
BspACI CCGC 2 cut(s) 1350, 1492
BspANI GGCC 3 cut(s) 318, 1256, 1430
BspCNI CTCAG 1 cut(s) 1592
BspDI ATCGAT 1 cut(s) 855
BspEI TCCGGA 2 cut(s) 221, 1090
BspHI TCATGA 1 cut(s) 1455
BspLI GGNNCC 3 cut(s) 593, 1172, 1308
BspMI ACCTGC 2 cut(s) 1117, 1494
BspPI GGATC 2 cut(s) 74, 851
BspT104I TTCGAA 1 cut(s) 1237
BspT107I GGYRCC 1 cut(s) 1306
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 4 cut(s) 200, 409, 1113, 1151
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 2 cut(s) 588, 1323
BssMI GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
BssT1I CCWWGG 1 cut(s) 1323
Bst2UI CCWGG 2 cut(s) 315, 589
Bst4CI ACNGT 4 cut(s) 24, 778, 1034, 1080
Bst6I CTCTTC 1 cut(s) 123
BstAPI GCANNNNNTGC 3 cut(s) 374, 932, 1505
BstBI TTCGAA 1 cut(s) 1237
BstC8I GCNNGC 5 cut(s) 370, 444, 483, 937, 1585
BstDEI CTNAG 6 cut(s) 602, 767, 1160, 1199, 1223, 1579
BstF5I GGATG 3 cut(s) 98, 919, 1346
BstHHI GCGC 1 cut(s) 1499
BstKTI GATC 7 cut(s) 47, 82, 304, 859, 1369, 1410, 1597
BstMBI GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
BstMWI GCNNNNNNNGC 6 cut(s) 374, 746, 923, 932, 1436, 1505
BstNI CCWGG 2 cut(s) 315, 589
BstNSI RCATGY 3 cut(s) 493, 1571, 1587
BstSCI CCNGG 3 cut(s) 313, 587, 1173
BstV1I GCAGC 7 cut(s) 433, 571, 789, 792, 904, 1025, 1476
BstXI CCANNNNNNTGG 2 cut(s) 692, 1011
Bsu15I ATCGAT 1 cut(s) 855
BsuRI GGCC 3 cut(s) 318, 1256, 1430
BsuTUI ATCGAT 1 cut(s) 855
BtgZI GCGATG 2 cut(s) 423, 1040
BtsCI GGATG 3 cut(s) 98, 919, 1346
BtsI GCAGTG 2 cut(s) 372, 846
BtsIMutI CAGTG 4 cut(s) 372, 846, 1076, 1120
BveI ACCTGC 2 cut(s) 1117, 1494
Cac8I GCNNGC 5 cut(s) 370, 444, 483, 937, 1585
CaiI CAGNNNCTG 1 cut(s) 1448
CciI TCATGA 1 cut(s) 1455
CfoI GCGC 1 cut(s) 1499
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 592
ClaI ATCGAT 1 cut(s) 855
Csp6I GTAC 2 cut(s) 552, 1390
CspCI CAANNNNNGTGG 2 cut(s) 1110, 1145
CviQI GTAC 2 cut(s) 552, 1390
DdeI CTNAG 6 cut(s) 602, 767, 1160, 1199, 1223, 1579
DpnI GATC 7 cut(s) 46, 81, 303, 858, 1368, 1409, 1596
DpnII GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
DraIII CACNNNGTG 1 cut(s) 875
DrdI GACNNNNNNGTC 1 cut(s) 733
DseDI GACNNNNNNGTC 1 cut(s) 733
EaeI YGGCCR 1 cut(s) 1254
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 1323
Eco147I AGGCCT 1 cut(s) 318
Eco32I GATATC 1 cut(s) 1616
Eco47I GGWCC 1 cut(s) 592
Eco57I CTGAAG 4 cut(s) 147, 479, 697, 976
EcoRII CCWGG 2 cut(s) 313, 587
EcoRV GATATC 1 cut(s) 1616
EcoT14I CCWWGG 1 cut(s) 1323
EcoT22I ATGCAT 2 cut(s) 1441, 1589
ErhI CCWWGG 1 cut(s) 1323
FaqI GGGAC 1 cut(s) 605
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 1366
FblI GTMKAC 2 cut(s) 1082, 1482
Fnu4HI GCNGC 7 cut(s) 447, 585, 803, 806, 918, 1039, 1490
FokI GGATG 3 cut(s) 85, 926, 1333
Fsp4HI GCNGC 7 cut(s) 447, 585, 803, 806, 918, 1039, 1490
FspBI CTAG 2 cut(s) 119, 1311
FspI TGCGCA 1 cut(s) 1498
GlaI GCGC 1 cut(s) 1498
GluI GCNGC 7 cut(s) 447, 585, 803, 806, 918, 1039, 1490
GsaI CCCAGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 1445
HaeIII GGCC 3 cut(s) 318, 1256, 1430
HapII CCGG 5 cut(s) 222, 443, 1091, 1165, 1174
HhaI GCGC 1 cut(s) 1499
Hin6I GCGC 1 cut(s) 1497
HinP1I GCGC 1 cut(s) 1497
HincII GTYRAC 2 cut(s) 1083, 1402
HindII GTYRAC 2 cut(s) 1083, 1402
HindIII AAGCTT 1 cut(s) 1228
HinfI GANTC 9 cut(s) 122, 218, 248, 269, 408, 696, 1087, 1118, 1459
HpaI GTTAAC 1 cut(s) 1402
HpaII CCGG 5 cut(s) 222, 443, 1091, 1165, 1174
HphI GGTGA 5 cut(s) 401, 747, 1288, 1335, 1394
Hpy166II GTNNAC 5 cut(s) 1076, 1083, 1135, 1402, 1483
Hpy188I TCNGA 3 cut(s) 127, 652, 787
Hpy188III TCNNGA 9 cut(s) 119, 136, 222, 724, 947, 1091, 1249, 1336, 1456
Hpy8I GTNNAC 5 cut(s) 1076, 1083, 1135, 1402, 1483
Hpy99I CGWCG 2 cut(s) 367, 1087
HpyAV CCTTC 3 cut(s) 74, 1000, 1540
HpyCH4III ACNGT 4 cut(s) 24, 778, 1034, 1080
HpyCH4IV ACGT 2 cut(s) 294, 1072
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 746, 923, 932, 1436, 1505
HpyF3I CTNAG 6 cut(s) 602, 767, 1160, 1199, 1223, 1579
HpySE526I ACGT 2 cut(s) 294, 1072
HspAI GCGC 1 cut(s) 1497
Kpn2I TCCGGA 2 cut(s) 221, 1090
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 1366
KspAI GTTAAC 1 cut(s) 1402
Kzo9I GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
Lsp1109I GCAGC 7 cut(s) 433, 571, 789, 792, 904, 1025, 1476
LweI GCATC 2 cut(s) 904, 1448
MaeI CTAG 2 cut(s) 119, 1311
MaeII ACGT 2 cut(s) 294, 1072
MaeIII GTNAC 2 cut(s) 35, 540
MalI GATC 7 cut(s) 46, 81, 303, 858, 1368, 1409, 1596
MboI GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
MboII GAAGA 3 cut(s) 140, 351, 1195
MfeI CAATTG 1 cut(s) 427
MlsI TGGCCA 1 cut(s) 1256
MluCI AATT 6 cut(s) 427, 609, 687, 743, 1418, 1572
MluNI TGGCCA 1 cut(s) 1256
MlyI GAGTC 2 cut(s) 116, 1453
MmeI TCCRAC 2 cut(s) 673, 1453
Mox20I TGGCCA 1 cut(s) 1256
Mph1103I ATGCAT 2 cut(s) 1441, 1589
MroI TCCGGA 2 cut(s) 221, 1090
MroNI GCCGGC 1 cut(s) 442
MscI TGGCCA 1 cut(s) 1256
MseI TTAA 4 cut(s) 206, 297, 1050, 1401
MslI CAYNNNNRTG 2 cut(s) 912, 1009
Msp20I TGGCCA 1 cut(s) 1256
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 5 cut(s) 222, 443, 1091, 1165, 1174
MspR9I CCNGG 3 cut(s) 315, 589, 1175
MunI CAATTG 1 cut(s) 427
MvaI CCWGG 2 cut(s) 315, 589
MwoI GCNNNNNNNGC 6 cut(s) 374, 746, 923, 932, 1436, 1505
NaeI GCCGGC 1 cut(s) 444
NciI CCSGG 1 cut(s) 1175
NdeI CATATG 1 cut(s) 922
NdeII GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
NgoMIV GCCGGC 1 cut(s) 442
NlaIV GGNNCC 3 cut(s) 593, 1172, 1308
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 1 cut(s) 35
NsbI TGCGCA 1 cut(s) 1498
NsiI ATGCAT 2 cut(s) 1441, 1589
NspI RCATGY 3 cut(s) 493, 1571, 1587
NspV TTCGAA 1 cut(s) 1237
PaeI GCATGC 1 cut(s) 1587
PagI TCATGA 1 cut(s) 1455
PaqCI CACCTGC 1 cut(s) 1117
PceI AGGCCT 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 444
PfeI GAWTC 7 cut(s) 218, 248, 269, 408, 696, 1087, 1118
PkrI GCNGC 7 cut(s) 448, 586, 804, 807, 919, 1040, 1491
PleI GAGTC 2 cut(s) 116, 1453
PpsI GAGTC 2 cut(s) 116, 1453
PsiI TTATAA 1 cut(s) 1377
Psp6I CCWGG 2 cut(s) 313, 587
PspFI CCCAGC 1 cut(s) 377
PspGI CCWGG 2 cut(s) 313, 587
PspN4I GGNNCC 3 cut(s) 593, 1172, 1308
PspPI GGNCC 1 cut(s) 592
PstNI CAGNNNCTG 1 cut(s) 1448
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 2 cut(s) 553, 1391
RsaNI GTAC 2 cut(s) 552, 1390
RseI CAYNNNNRTG 2 cut(s) 912, 1009
SalI GTCGAC 1 cut(s) 1081
SaqAI TTAA 4 cut(s) 206, 297, 1050, 1401
SatI GCNGC 7 cut(s) 447, 585, 803, 806, 918, 1039, 1490
Sau3AI GATC 7 cut(s) 44, 79, 301, 856, 1366, 1407, 1594
Sau96I GGNCC 1 cut(s) 592
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 2 cut(s) 116, 1453
ScrFI CCNGG 3 cut(s) 315, 589, 1175
SfaNI GCATC 2 cut(s) 904, 1448
SfuI TTCGAA 1 cut(s) 1237
SinI GGWCC 1 cut(s) 592
SmiMI CAYNNNNRTG 2 cut(s) 912, 1009
SphI GCATGC 1 cut(s) 1587
Sse9I AATT 6 cut(s) 427, 609, 687, 743, 1418, 1572
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 2 cut(s) 1350, 1492
SspMI CTAG 2 cut(s) 119, 1311
StuI AGGCCT 1 cut(s) 318
StyD4I CCNGG 3 cut(s) 313, 587, 1173
StyI CCWWGG 1 cut(s) 1323
TaaI ACNGT 4 cut(s) 24, 778, 1034, 1080
TaiI ACGT 2 cut(s) 297, 1075
TaqI TCGA 7 cut(s) 43, 362, 459, 855, 1082, 1237, 1536
TasI AATT 6 cut(s) 427, 609, 687, 743, 1418, 1572
TatI WGTACW 2 cut(s) 551, 1389
TfiI GAWTC 7 cut(s) 218, 248, 269, 408, 696, 1087, 1118
Tru1I TTAA 4 cut(s) 206, 297, 1050, 1401
Tru9I TTAA 4 cut(s) 206, 297, 1050, 1401
TscAI CASTG 4 cut(s) 379, 853, 1083, 1120
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 7 cut(s) 446, 584, 802, 805, 917, 1038, 1489
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 6 cut(s) 154, 426, 503, 896, 952, 1444
TspRI CASTG 4 cut(s) 379, 853, 1083, 1120
VpaK11BI GGWCC 1 cut(s) 592
XapI RAATTY 1 cut(s) 743
XbaI TCTAGA 1 cut(s) 118
XceI RCATGY 3 cut(s) 493, 1571, 1587
XmiI GTMKAC 2 cut(s) 1082, 1482
XspI CTAG 2 cut(s) 119, 1311
ZrmI AGTACT 1 cut(s) 553
Zsp2I ATGCAT 2 cut(s) 1441, 1589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.