Rroxscaffold_1G00061490

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001466
Physical Location & Seq
Forward (+)
83608421 .. 83609961
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061490.1

Sequence Viewer

Length: 1461 bp
ATGGGTAGCCTCGAATTCCACCATGGTCCAGTAGAAAACAACTGTGATCCCCCCATGGCTAACCCTCTGGATCCGGAAGAATTCAGGAGACAAGGCCACATAATGATAGATTTTATTGCAGACTATTACAAGAACATAGAGAAGTACCCGGTTCTAAGCCAAGTTCAACCAGGTTATCTCAAAAAGCGCCTGCCGGAGTCTGCGCCATACGACCCCGAGCCGATTGAAACCATCCTCCAAGATGTGCGAGATCATATTGTTCCTGGTATAACTCATTGGCAAAGCCCAAGCTACTTTGGCTACTTTCCTTCAAGTGGTAGCATCGCAGGGTTTCTAGGCGAAATGCTTAGCAGTGGATTCAATGTGGTTGGATTCAACTGGATGTCATCGCCTGCTGCAACTGAGCTAGAAAGCATAGTCATGGATTGGCTTGGAAACATGCTTCAACTTCCCAAGTCTTTCCTTTTCTCTGGCAACGGTGGTGGTGTGTTACAAGGGACTACTTGTGAGGCCATTGTTTGCACCATGGCTGCTGCTAGAGACCAAATGCTAAGCCGAATTGGTAAAGACAACATTGGGAGGCTAGTTGTATATGGGTCAGATCAAACACATAGTGCACTTAAAAAAGCCTCCCAAATTGTTGGAATCCATCCAAACAATTTCAGGGCCATCAACACTACACAATCCACAAATTTTGCACTATCACCTGAGTTGCTGAGATCCACAATTTGTTCTGACATAGAAAAAGGGTCGGTTCCTTTGTTTCTATGTGCCACGATGGGAACAACTGCAACCACTTCTGTTGATCCATTGGGGGGACTGTGTGATGTAGCAAAAGATTATGGCATATGGGTTCATGTCGATGCAGCATACGCAGGAAGTGTCTGCATTTGTCAAGGCGTTGAGGGAGCAAATTCATTGAAGGCGTTGAGGGAGCAAATTCATTCAGTTTCAATGCACATAAATGAGTTTTTAAGGAACAAAGCCAGTGATTCAAAGCAAGTGGTGGACTACAAAGACTGGCAAATAGCACTGAGCCGAAGGTTCCGCGCATTGAAATTATGGCTCGTGCTAAGAAGCTATGGAGTAGATAATCTCAGAAACTTTCTCAGAAGCCATGTGAAAATGGCCGAGGTCTTTGAAAGGCTTGTGCGAGAGAACAAATGGTTTGAGGTTGTTGTGCCTAGGAATTTCGCCATGGTCTGTTTTAGGATTTCTCCATCAGCAATTAGCGAAACTCCACGTGATGATGATGGAAGTGCTATGATAAACGAGGTCAATTGCAAGCTATTGGAGGCGATCAATGCATCAGGTGATGTATACATGACTCATGCTGTGGTTGGAGGCATGTGTGTGTTACGGTGCGCAATTGGGGCAACTCTGACCGAGGAAAAGCACATACACAGGGCCTGGAAGGTAGTGCAAGACCATGCAGATGCCATCCTGAGCACGTATCAATAG

Protein Analysis

486

Amino Acids

53.85

Weight (kDa)

6.02

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 57 - 303 5.3e-66 Pyridoxal-dependent decarboxylase conserved domain
Pyridoxal_deC PF00282 318 - 405 6.1e-20 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1366
AccI GTMKAC 1 cut(s) 1320
AccII CGCG 1 cut(s) 1050
AccIII TCCGGA 1 cut(s) 73
AciI CCGC 1 cut(s) 1048
AclWI GGATC 5 cut(s) 41, 65, 78, 714, 800
AcoI YGGCCR 1 cut(s) 1128
AcsI RAATTY 6 cut(s) 14, 80, 691, 913, 939, 1189
AcvI CACGTG 1 cut(s) 1244
AdeI CACNNNGTG 1 cut(s) 614
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 2 cut(s) 314, 815
AjnI CCWGG 3 cut(s) 169, 262, 1409
AluBI AGCT 4 cut(s) 291, 406, 1080, 1288
AluI AGCT 4 cut(s) 291, 406, 1080, 1288
Alw21I GWGCWC 2 cut(s) 619, 1451
Alw26I GTCTC 2 cut(s) 82, 534
Alw44I GTGCAC 1 cut(s) 615
AlwI GGATC 5 cut(s) 41, 65, 78, 714, 800
AlwNI CAGNNNCTG 1 cut(s) 1410
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 73
AoxI GGCC 5 cut(s) 94, 510, 666, 1128, 1407
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 4 cut(s) 395, 530, 533, 866
ApoI RAATTY 6 cut(s) 14, 80, 691, 913, 939, 1189
AspA2I CCTAGG 1 cut(s) 1184
AspLEI GCGC 4 cut(s) 189, 205, 1052, 1367
AspS9I GGNCC 3 cut(s) 26, 666, 1407
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 696, 1325
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 26
AvrII CCTAGG 1 cut(s) 1184
BaeGI GKGCMC 1 cut(s) 619
BamHI GGATCC 1 cut(s) 70
BauI CACGAG 1 cut(s) 1067
BbrPI CACGTG 1 cut(s) 1244
Bbv12I GWGCWC 2 cut(s) 619, 1451
BbvI GCAGC 4 cut(s) 382, 517, 520, 878
BccI CCATC 7 cut(s) 239, 657, 677, 772, 1228, 1247, 1448
BciT130I CCWGG 3 cut(s) 171, 264, 1411
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 82, 534
BfaI CTAG 5 cut(s) 335, 407, 537, 584, 1185
BfoI RGCGCY 1 cut(s) 190
BisI GCNGC 4 cut(s) 396, 531, 534, 867
BlnI CCTAGG 1 cut(s) 1184
BlpI GCTNAGC 2 cut(s) 347, 551
BlsI GCNGC 4 cut(s) 397, 532, 535, 868
Bme1390I CCNGG 4 cut(s) 149, 171, 264, 1411
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 3 cut(s) 26, 666, 1407
BmiI GGNNCC 3 cut(s) 72, 756, 1046
BmrFI CCNGG 4 cut(s) 149, 171, 264, 1411
BmsI GCATC 4 cut(s) 330, 853, 1316, 1426
Bpu10I CCTNAGC 1 cut(s) 1445
Bpu1102I GCTNAGC 2 cut(s) 347, 551
BpuMI CCSGG 1 cut(s) 149
BsaAI YACGTR 2 cut(s) 1244, 1452
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 7 cut(s) 22, 54, 525, 1131, 1184, 1197, 1386
BsaWI WCCGGW 1 cut(s) 73
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 815
Bse1I ACTGG 4 cut(s) 29, 383, 987, 1025
BseAI TCCGGA 1 cut(s) 73
BseBI CCWGG 3 cut(s) 171, 264, 1411
BseDI CCNNGG 7 cut(s) 22, 54, 525, 1131, 1184, 1197, 1386
BseGI GGATG 4 cut(s) 231, 387, 649, 1440
BseLI CCNNNNNNNGG 2 cut(s) 314, 815
BseMII CTCAG 7 cut(s) 393, 699, 707, 1025, 1111, 1123, 1436
BseNI ACTGG 4 cut(s) 29, 383, 987, 1025
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 4 cut(s) 382, 517, 520, 878
Bsh1236I CGCG 1 cut(s) 1050
BshFI GGCC 5 cut(s) 96, 512, 668, 1130, 1409
BsiHKAI GWGCWC 2 cut(s) 619, 1451
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 3 cut(s) 74, 149, 194
BslFI GGGAC 2 cut(s) 511, 831
BslI CCNNNNNNNGG 2 cut(s) 314, 815
BsmAI GTCTC 2 cut(s) 82, 534
BsmFI GGGAC 2 cut(s) 511, 831
BsnI GGCC 5 cut(s) 96, 512, 668, 1130, 1409
Bso31I GGTCTC 1 cut(s) 534
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 619, 1451
Bsp13I TCCGGA 1 cut(s) 73
Bsp143I GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
Bsp1720I GCTNAGC 2 cut(s) 347, 551
Bsp19I CCATGG 4 cut(s) 22, 54, 525, 1197
BspACI CCGC 1 cut(s) 1048
BspANI GGCC 5 cut(s) 96, 512, 668, 1130, 1409
BspCNI CTCAG 7 cut(s) 394, 700, 708, 1026, 1110, 1122, 1437
BspEI TCCGGA 1 cut(s) 73
BspFNI CGCG 1 cut(s) 1050
BspLI GGNNCC 3 cut(s) 72, 756, 1046
BspPI GGATC 5 cut(s) 41, 65, 78, 714, 800
BspTNI GGTCTC 1 cut(s) 534
BsrI ACTGG 4 cut(s) 29, 383, 987, 1025
BssECI CCNNGG 7 cut(s) 22, 54, 525, 1131, 1184, 1197, 1386
BssMI GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
BssNAI GTATAC 1 cut(s) 1321
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 5 cut(s) 22, 54, 525, 1184, 1197
Bst1107I GTATAC 1 cut(s) 1321
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 3 cut(s) 171, 264, 1411
Bst4CI ACNGT 4 cut(s) 44, 479, 822, 1362
BstBAI YACGTR 2 cut(s) 1244, 1452
BstC8I GCNNGC 3 cut(s) 191, 393, 1286
BstDSI CCRYGG 4 cut(s) 22, 54, 525, 1197
BstF5I GGATG 4 cut(s) 231, 387, 649, 1440
BstFNI CGCG 1 cut(s) 1050
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 4 cut(s) 189, 205, 1052, 1367
BstKTI GATC 7 cut(s) 49, 73, 253, 604, 722, 808, 1302
BstMAI GTCTC 2 cut(s) 82, 534
BstMBI GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
BstMWI GCNNNNNNNGC 4 cut(s) 297, 872, 1304, 1373
BstNI CCWGG 3 cut(s) 171, 264, 1411
BstNSI RCATGY 2 cut(s) 442, 1351
BstSCI CCNGG 4 cut(s) 147, 169, 262, 1409
BstSLI GKGCMC 1 cut(s) 619
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 4 cut(s) 382, 517, 520, 878
BstX2I RGATCY 2 cut(s) 70, 719
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 2 cut(s) 70, 719
BstZ17I GTATAC 1 cut(s) 1321
BsuRI GGCC 5 cut(s) 96, 512, 668, 1130, 1409
BtgI CCRYGG 4 cut(s) 22, 54, 525, 1197
BtgZI GCGATG 2 cut(s) 307, 372
BtsCI GGATG 4 cut(s) 231, 387, 649, 1440
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 3 cut(s) 358, 994, 1031
Cac8I GCNNGC 3 cut(s) 191, 393, 1286
CaiI CAGNNNCTG 1 cut(s) 1410
CfoI GCGC 4 cut(s) 189, 205, 1052, 1367
Cfr13I GGNCC 3 cut(s) 26, 666, 1407
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 1 cut(s) 145
CspCI CAANNNNNGTGG 8 cut(s) 463, 498, 676, 711, 784, 819, 984, 1019
CviQI GTAC 1 cut(s) 145
DpnI GATC 7 cut(s) 48, 72, 252, 603, 721, 807, 1301
DpnII GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
DraIII CACNNNGTG 1 cut(s) 614
EaeI YGGCCR 1 cut(s) 1128
Eco130I CCWWGG 5 cut(s) 22, 54, 525, 1184, 1197
Eco31I GGTCTC 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 26
Eco72I CACGTG 1 cut(s) 1244
Eco88I CYCGRG 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 1407
EcoRI GAATTC 2 cut(s) 14, 80
EcoRII CCWGG 3 cut(s) 169, 262, 1409
EcoT14I CCWWGG 5 cut(s) 22, 54, 525, 1184, 1197
EcoT22I ATGCAT 1 cut(s) 1309
ErhI CCWWGG 5 cut(s) 22, 54, 525, 1184, 1197
FaqI GGGAC 2 cut(s) 511, 831
FauNDI CATATG 1 cut(s) 848
FblI GTMKAC 1 cut(s) 1320
Fnu4HI GCNGC 4 cut(s) 396, 531, 534, 867
FokI GGATG 4 cut(s) 218, 394, 636, 1427
Fsp4HI GCNGC 4 cut(s) 396, 531, 534, 867
FspBI CTAG 5 cut(s) 335, 407, 537, 584, 1185
FspI TGCGCA 1 cut(s) 1366
GlaI GCGC 4 cut(s) 188, 204, 1051, 1366
GluI GCNGC 4 cut(s) 396, 531, 534, 867
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 5 cut(s) 96, 512, 668, 1130, 1409
HapII CCGG 3 cut(s) 74, 149, 194
HhaI GCGC 4 cut(s) 189, 205, 1052, 1367
Hin6I GCGC 4 cut(s) 187, 203, 1050, 1365
HinP1I GCGC 4 cut(s) 187, 203, 1050, 1365
HinfI GANTC 6 cut(s) 197, 357, 372, 645, 992, 1327
HpaII CCGG 3 cut(s) 74, 149, 194
HphI GGTGA 2 cut(s) 696, 1325
Hpy166II GTNNAC 3 cut(s) 617, 1009, 1321
Hpy188I TCNGA 5 cut(s) 601, 736, 1100, 1112, 1383
Hpy188III TCNNGA 4 cut(s) 68, 74, 85, 1444
Hpy8I GTNNAC 3 cut(s) 617, 1009, 1321
HpyAV CCTTC 4 cut(s) 318, 916, 1035, 1408
HpyCH4III ACNGT 4 cut(s) 44, 479, 822, 1362
HpyCH4IV ACGT 2 cut(s) 1243, 1451
HpyF10VI GCNNNNNNNGC 4 cut(s) 297, 872, 1304, 1373
HpySE526I ACGT 2 cut(s) 1243, 1451
HspAI GCGC 4 cut(s) 187, 203, 1050, 1365
Kpn2I TCCGGA 1 cut(s) 73
Kzo9I GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
LmnI GCTCC 2 cut(s) 908, 934
Lsp1109I GCAGC 4 cut(s) 382, 517, 520, 878
LweI GCATC 4 cut(s) 330, 853, 1316, 1426
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 5 cut(s) 335, 407, 537, 584, 1185
MaeII ACGT 2 cut(s) 1243, 1451
MaeIII GTNAC 2 cut(s) 489, 1356
MalI GATC 7 cut(s) 48, 72, 252, 603, 721, 807, 1301
MboI GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
MboII GAAGA 1 cut(s) 89
MfeI CAATTG 2 cut(s) 1279, 1368
MflI RGATCY 2 cut(s) 70, 719
MhlI GDGCHC 2 cut(s) 619, 1451
MlyI GAGTC 2 cut(s) 206, 1321
MmeI TCCRAC 3 cut(s) 349, 622, 1321
Mph1103I ATGCAT 1 cut(s) 1309
MroI TCCGGA 1 cut(s) 73
MseI TTAA 2 cut(s) 621, 974
MslI CAYNNNNRTG 5 cut(s) 419, 861, 963, 1352, 1434
MspI CCGG 3 cut(s) 74, 149, 194
MspR9I CCNGG 4 cut(s) 149, 171, 264, 1411
MunI CAATTG 2 cut(s) 1279, 1368
MvaI CCWGG 3 cut(s) 171, 264, 1411
MvnI CGCG 1 cut(s) 1050
MwoI GCNNNNNNNGC 4 cut(s) 297, 872, 1304, 1373
NciI CCSGG 1 cut(s) 149
NcoI CCATGG 4 cut(s) 22, 54, 525, 1197
NdeI CATATG 1 cut(s) 848
NdeII GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
NlaIV GGNNCC 3 cut(s) 72, 756, 1046
NmeAIII GCCGAG 1 cut(s) 1156
NsbI TGCGCA 1 cut(s) 1366
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 2 cut(s) 442, 1351
PfeI GAWTC 4 cut(s) 357, 372, 645, 992
PkrI GCNGC 4 cut(s) 397, 532, 535, 868
PleI GAGTC 2 cut(s) 205, 1321
PmaCI CACGTG 1 cut(s) 1244
PmlI CACGTG 1 cut(s) 1244
PpsI GAGTC 2 cut(s) 205, 1321
Ppu21I YACGTR 2 cut(s) 1244, 1452
Psp6I CCWGG 3 cut(s) 169, 262, 1409
PspCI CACGTG 1 cut(s) 1244
PspGI CCWGG 3 cut(s) 169, 262, 1409
PspN4I GGNNCC 3 cut(s) 72, 756, 1046
PspPI GGNCC 3 cut(s) 26, 666, 1407
PstNI CAGNNNCTG 1 cut(s) 1410
PsuI RGATCY 2 cut(s) 70, 719
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 5 cut(s) 419, 861, 963, 1352, 1434
SaqAI TTAA 2 cut(s) 621, 974
SatI GCNGC 4 cut(s) 396, 531, 534, 867
Sau3AI GATC 7 cut(s) 46, 70, 250, 601, 719, 805, 1299
Sau96I GGNCC 3 cut(s) 26, 666, 1407
SchI GAGTC 2 cut(s) 206, 1321
ScrFI CCNGG 4 cut(s) 149, 171, 264, 1411
SduI GDGCHC 2 cut(s) 619, 1451
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 4 cut(s) 330, 853, 1316, 1426
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 5 cut(s) 419, 861, 963, 1352, 1434
SsiI CCGC 1 cut(s) 1048
SspMI CTAG 5 cut(s) 335, 407, 537, 584, 1185
StyD4I CCNGG 4 cut(s) 147, 169, 262, 1409
StyI CCWWGG 5 cut(s) 22, 54, 525, 1184, 1197
TaaI ACNGT 4 cut(s) 44, 479, 822, 1362
TaiI ACGT 2 cut(s) 1246, 1454
TaqI TCGA 2 cut(s) 12, 861
TaqII GACCGA 1 cut(s) 1400
TfiI GAWTC 4 cut(s) 357, 372, 645, 992
Tru1I TTAA 2 cut(s) 621, 974
Tru9I TTAA 2 cut(s) 621, 974
TscAI CASTG 3 cut(s) 358, 994, 1038
TseI GCWGC 4 cut(s) 395, 530, 533, 866
TspDTI ATGAA 3 cut(s) 845, 906, 932
TspRI CASTG 3 cut(s) 358, 994, 1038
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 6 cut(s) 14, 80, 691, 913, 939, 1189
XceI RCATGY 2 cut(s) 442, 1351
XmaJI CCTAGG 1 cut(s) 1184
XmiI GTMKAC 1 cut(s) 1320
XspI CTAG 5 cut(s) 335, 407, 537, 584, 1185
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.