Rroxscaffold_1G00061480

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001466
Physical Location & Seq
Forward (+)
83592674 .. 83593882
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061480.1

Sequence Viewer

Length: 1209 bp
ATGCTCATAACTGGGTTCAATGTCGTGGGGTTCAATTGGGTTTCATCGCCGGCTGCAACCGAGCTCGAACACATAGTCATGGATTGGCTCGCAGACATGCTTCAGCTTCCCAAGTCGTTTCATTTCTCTGGTAACGGTGGCGGTGTGTTACATGGGAGTACTTGCGAAGCCATTGTTTGCACAATGGCAGCTGCCAGGGACCAAATGCTAAGCCGAATTGGTAGCGAGAATTTGGGAAAGCTTGTTGTGTATGGTTCAGACCAAACACATTGCACCCTTCAAAAAGCTACCCAAATTGTTGGAATCAACCCGGAGAACTTCAGGGCCATCAAGACTACAAAGTCAACTGGATTTGCACTATCACCTGAAATGCTAAGACTGACAATAAGTTCAGACAGAGCAAAAGGTCTAGTGCCACTTTTTCTATGTGCCACAATGGGCGCTACCGCAACCACTGCTATAGATCCATTAGGGGCAGTCTGCAATGTAGCCAAAGATTATGGCATGTGGGTACATGTGGACGCCGCATATGCAGGAAGTGCTTGCATTTGTCCAGAGTTTCGTCATTTCATTGATGGCGTTGAGGGTGCAAACTCATTTAGTTTCAACCCGCATAAATGGTTATTTACTGCAATGGACTGTTGCTGCCTTTGGGTTAAGAGCCCAAGTGCATTAATAAAGTCACTGTCGACGAATCCAGAGTATTTGAGGAACAAAGCCAGTGATACAAAGCAAGTTGTGGACTACAAAAACTGGCAAATAGCACTAAGCCGGAGGTTCCGGGCATTGAAGCTATGGCTTGTGCTAAGAAGCTACGGCGTGGCTAACCTTAGAAAATTTATTCGAAGCCACGTCAAAATGGCCAAGATTTTTGAAGGGCTTGTGAGAATGGACAATAGGTTTGAGATTGTGGTTCCTAGAAACTTCTCCTTGGTCTGTTTCAGGATTTCACTATCCGCTATAGTTAGTGATGATCATTATGATGGTGAGGAGTACAATATGGTAAACGAGATCAATCGCAAGTTGCTGGAGGCCATCAATGCATCAGGCTCTGTGTTCATGACTCATGCTGTGGTTGGAGGTGTCTACGTGCTACGGTGCGCAGTTGGTGCAACTCTAACCGAGGAAAAGCACATTCGAGAGGCCTGGAAGGCGGTGCAAGGCCAGGCACATACAATTCTCTCAGCATGTATTGTTGACTCTGCATAA

Protein Analysis

402

Amino Acids

44.27

Weight (kDa)

8.51

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 1 - 316 7.5e-93 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 340
Acc16I TGCGCA 1 cut(s) 1102
AccI GTMKAC 2 cut(s) 689, 1086
AciI CCGC 6 cut(s) 141, 447, 525, 611, 957, 1154
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 2 cut(s) 229, 836
AcuI CTGAAG 2 cut(s) 86, 304
AcyI GRCGYC 1 cut(s) 522
AfaI GTAC 3 cut(s) 160, 513, 995
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 6 cut(s) 19, 34, 281, 607, 790, 875
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 3 cut(s) 194, 1145, 1164
AluBI AGCT 7 cut(s) 64, 106, 191, 241, 287, 793, 813
AluI AGCT 7 cut(s) 64, 106, 191, 241, 287, 793, 813
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 1052
AoxI GGCC 5 cut(s) 324, 861, 1032, 1143, 1162
ApeKI GCWGC 4 cut(s) 53, 188, 191, 645
ApoI RAATTY 2 cut(s) 229, 836
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 884, 916
AseI ATTAAT 1 cut(s) 674
AspLEI GCGC 2 cut(s) 443, 1103
AspS9I GGNCC 2 cut(s) 199, 324
AsuC2I CCSGG 2 cut(s) 311, 782
AsuHPI GGTGA 2 cut(s) 354, 998
AsuII TTCGAA 1 cut(s) 844
AvaII GGWCC 1 cut(s) 199
BalI TGGCCA 1 cut(s) 863
BanII GRGCYC 2 cut(s) 66, 665
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 4 cut(s) 40, 178, 200, 632
BccI CCATC 4 cut(s) 335, 569, 977, 1043
BceAI ACGGC 1 cut(s) 832
BciT130I CCWGG 3 cut(s) 196, 1147, 1166
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 311, 782
BfaI CTAG 2 cut(s) 410, 918
BfmI CTRYAG 2 cut(s) 459, 960
BfoI RGCGCY 1 cut(s) 444
BglI GCCNNNNNGGC 1 cut(s) 1151
BisI GCNGC 5 cut(s) 54, 189, 192, 525, 646
BlpI GCTNAGC 1 cut(s) 209
BlsI GCNGC 5 cut(s) 55, 190, 193, 526, 647
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 5 cut(s) 196, 311, 782, 1147, 1166
Bme18I GGWCC 1 cut(s) 199
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 2 cut(s) 199, 324
BmiI GGNNCC 3 cut(s) 200, 779, 915
BmrFI CCNGG 5 cut(s) 196, 311, 782, 1147, 1166
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 1052
BmuI ACTGGG 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 1049
Bpu1102I GCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 844
BpuMI CCSGG 2 cut(s) 311, 782
BsaAI YACGTR 1 cut(s) 1090
BsaHI GRCGYC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 195, 930, 1122
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 4 cut(s) 16, 352, 720, 758
Bse3DI GCAATG 3 cut(s) 268, 490, 639
BseBI CCWGG 3 cut(s) 196, 1147, 1166
BseDI CCNNGG 3 cut(s) 195, 930, 1122
BseMI GCAATG 3 cut(s) 268, 490, 639
BseMII CTCAG 1 cut(s) 1197
BseNI ACTGG 4 cut(s) 16, 352, 720, 758
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 4 cut(s) 40, 178, 200, 632
BshFI GGCC 5 cut(s) 326, 863, 1034, 1145, 1164
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 4 cut(s) 50, 311, 772, 781
BslFI GGGAC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 5 cut(s) 326, 863, 1034, 1145, 1164
Bsp119I TTCGAA 1 cut(s) 844
Bsp1286I GDGCHC 2 cut(s) 66, 665
Bsp143I GATC 3 cut(s) 463, 973, 1011
Bsp1720I GCTNAGC 1 cut(s) 209
BspACI CCGC 6 cut(s) 141, 447, 525, 611, 957, 1154
BspANI GGCC 5 cut(s) 326, 863, 1034, 1145, 1164
BspCNI CTCAG 1 cut(s) 1196
BspHI TCATGA 1 cut(s) 1059
BspLI GGNNCC 3 cut(s) 200, 779, 915
BspPI GGATC 1 cut(s) 458
BspT104I TTCGAA 1 cut(s) 844
BsrDI GCAATG 3 cut(s) 268, 490, 639
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 4 cut(s) 16, 352, 720, 758
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 3 cut(s) 195, 930, 1122
BssMI GATC 3 cut(s) 463, 973, 1011
BssNI GRCGYC 1 cut(s) 522
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 3 cut(s) 196, 1147, 1166
Bst4CI ACNGT 4 cut(s) 137, 641, 687, 1098
BstACI GRCGYC 1 cut(s) 522
BstAPI GCANNNNNTGC 3 cut(s) 455, 539, 1109
BstBAI YACGTR 1 cut(s) 1090
BstBI TTCGAA 1 cut(s) 844
BstC8I GCNNGC 3 cut(s) 51, 90, 544
BstDEI CTNAG 6 cut(s) 209, 374, 767, 806, 830, 1183
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 2 cut(s) 443, 1103
BstKTI GATC 3 cut(s) 466, 976, 1014
BstMBI GATC 3 cut(s) 463, 973, 1011
BstMWI GCNNNNNNNGC 6 cut(s) 455, 530, 539, 1040, 1109, 1151
BstNI CCWGG 3 cut(s) 196, 1147, 1166
BstNSI RCATGY 4 cut(s) 100, 508, 518, 1191
BstSCI CCNGG 5 cut(s) 194, 309, 780, 1145, 1164
BstSFI CTRYAG 2 cut(s) 459, 960
BstV1I GCAGC 4 cut(s) 40, 178, 200, 632
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 1 cut(s) 299
BstYI RGATCY 1 cut(s) 463
BsuRI GGCC 5 cut(s) 326, 863, 1034, 1145, 1164
BtgZI GCGATG 1 cut(s) 30
BtrI CACGTC 1 cut(s) 853
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 3 cut(s) 453, 683, 727
Cac8I GCNNGC 3 cut(s) 51, 90, 544
CaiI CAGNNNCTG 1 cut(s) 1052
CciI TCATGA 1 cut(s) 1059
CfoI GCGC 2 cut(s) 443, 1103
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 2 cut(s) 199, 324
CseI GACGC 1 cut(s) 530
Csp6I GTAC 3 cut(s) 159, 512, 994
CviAII CATG 8 cut(s) 79, 97, 152, 505, 515, 1060, 1067, 1188
CviQI GTAC 3 cut(s) 159, 512, 994
DdeI CTNAG 6 cut(s) 209, 374, 767, 806, 830, 1183
DpnI GATC 3 cut(s) 465, 975, 1013
DpnII GATC 3 cut(s) 463, 973, 1011
DrdI GACNNNNNNGTC 1 cut(s) 340
DseDI GACNNNNNNGTC 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 861
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 930
Eco147I AGGCCT 1 cut(s) 1145
Eco24I GRGCYC 2 cut(s) 66, 665
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 86, 304
EcoICRI GAGCTC 1 cut(s) 64
EcoRII CCWGG 3 cut(s) 194, 1145, 1164
EcoT14I CCWWGG 1 cut(s) 930
EcoT22I ATGCAT 1 cut(s) 1045
EcoT38I GRGCYC 2 cut(s) 66, 665
ErhI CCWWGG 1 cut(s) 930
FaeI CATG 8 cut(s) 82, 100, 155, 508, 518, 1063, 1070, 1191
FaqI GGGAC 1 cut(s) 212
FatI CATG 8 cut(s) 78, 96, 151, 504, 514, 1059, 1066, 1187
FauI CCCGC 1 cut(s) 618
FauNDI CATATG 1 cut(s) 529
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 2 cut(s) 689, 1086
Fnu4HI GCNGC 5 cut(s) 54, 189, 192, 525, 646
FriOI GRGCYC 2 cut(s) 66, 665
Fsp4HI GCNGC 5 cut(s) 54, 189, 192, 525, 646
FspBI CTAG 2 cut(s) 410, 918
FspI TGCGCA 1 cut(s) 1102
GlaI GCGC 2 cut(s) 442, 1102
GluI GCNGC 5 cut(s) 54, 189, 192, 525, 646
GsuI CTGGAG 1 cut(s) 1049
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 5 cut(s) 326, 863, 1034, 1145, 1164
HapII CCGG 4 cut(s) 50, 311, 772, 781
HgaI GACGC 1 cut(s) 530
HhaI GCGC 2 cut(s) 443, 1103
Hin1I GRCGYC 1 cut(s) 522
Hin1II CATG 8 cut(s) 82, 100, 155, 508, 518, 1063, 1070, 1191
Hin6I GCGC 2 cut(s) 441, 1101
HinP1I GCGC 2 cut(s) 441, 1101
HincII GTYRAC 3 cut(s) 345, 690, 1198
HindII GTYRAC 3 cut(s) 345, 690, 1198
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 4 cut(s) 303, 694, 1063, 1199
HpaII CCGG 4 cut(s) 50, 311, 772, 781
HphI GGTGA 2 cut(s) 354, 998
Hpy166II GTNNAC 7 cut(s) 345, 520, 690, 742, 1006, 1087, 1198
Hpy188I TCNGA 2 cut(s) 259, 394
Hpy188III TCNNGA 6 cut(s) 331, 554, 698, 943, 1060, 1139
Hpy8I GTNNAC 7 cut(s) 345, 520, 690, 742, 1006, 1087, 1198
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 3 cut(s) 287, 869, 1144
HpyCH4III ACNGT 4 cut(s) 137, 641, 687, 1098
HpyCH4IV ACGT 2 cut(s) 852, 1089
HpyF10VI GCNNNNNNNGC 6 cut(s) 455, 530, 539, 1040, 1109, 1151
HpyF3I CTNAG 6 cut(s) 209, 374, 767, 806, 830, 1183
HpySE526I ACGT 2 cut(s) 852, 1089
Hsp92I GRCGYC 1 cut(s) 522
Hsp92II CATG 8 cut(s) 82, 100, 155, 508, 518, 1063, 1070, 1191
HspAI GCGC 2 cut(s) 441, 1101
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 3 cut(s) 463, 973, 1011
Lsp1109I GCAGC 4 cut(s) 40, 178, 200, 632
LweI GCATC 1 cut(s) 1052
MaeI CTAG 2 cut(s) 410, 918
MaeII ACGT 2 cut(s) 852, 1089
MaeIII GTNAC 3 cut(s) 131, 147, 681
MalI GATC 3 cut(s) 465, 975, 1013
MboI GATC 3 cut(s) 463, 973, 1011
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 2 cut(s) 66, 665
MlsI TGGCCA 1 cut(s) 863
MluCI AATT 6 cut(s) 34, 216, 229, 294, 836, 1176
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 2 cut(s) 1057, 1193
MmeI TCCRAC 2 cut(s) 280, 1057
MnlI CCTC 8 cut(s) 577, 702, 768, 982, 1024, 1073, 1117, 1135
Mox20I TGGCCA 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 1045
MroNI GCCGGC 1 cut(s) 49
MscI TGGCCA 1 cut(s) 863
MseI TTAA 2 cut(s) 657, 674
MslI CAYNNNNRTG 2 cut(s) 77, 981
Msp20I TGGCCA 1 cut(s) 863
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 4 cut(s) 50, 311, 772, 781
MspR9I CCNGG 5 cut(s) 196, 311, 782, 1147, 1166
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 3 cut(s) 196, 1147, 1166
MwoI GCNNNNNNNGC 6 cut(s) 455, 530, 539, 1040, 1109, 1151
NaeI GCCGGC 1 cut(s) 51
NciI CCSGG 2 cut(s) 311, 782
NdeI CATATG 1 cut(s) 529
NdeII GATC 3 cut(s) 463, 973, 1011
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 8 cut(s) 82, 100, 155, 508, 518, 1063, 1070, 1191
NlaIV GGNNCC 3 cut(s) 200, 779, 915
NmuCI GTSAC 1 cut(s) 681
NsbI TGCGCA 1 cut(s) 1102
NsiI ATGCAT 1 cut(s) 1045
NspI RCATGY 4 cut(s) 100, 508, 518, 1191
NspV TTCGAA 1 cut(s) 844
PagI TCATGA 1 cut(s) 1059
PceI AGGCCT 1 cut(s) 1145
PciI ACATGT 1 cut(s) 514
PdiI GCCGGC 1 cut(s) 51
PfeI GAWTC 2 cut(s) 303, 694
PkrI GCNGC 5 cut(s) 55, 190, 193, 526, 647
PleI GAGTC 2 cut(s) 1057, 1193
PpsI GAGTC 2 cut(s) 1057, 1193
Ppu21I YACGTR 1 cut(s) 1090
PscI ACATGT 1 cut(s) 514
PshBI ATTAAT 1 cut(s) 674
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 194, 1145, 1164
PspGI CCWGG 3 cut(s) 194, 1145, 1164
PspN4I GGNNCC 3 cut(s) 200, 779, 915
PspPI GGNCC 2 cut(s) 199, 324
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 1 cut(s) 463
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 3 cut(s) 160, 513, 995
RsaNI GTAC 3 cut(s) 159, 512, 994
RseI CAYNNNNRTG 2 cut(s) 77, 981
SacI GAGCTC 1 cut(s) 66
SalI GTCGAC 1 cut(s) 688
SaqAI TTAA 2 cut(s) 657, 674
SatI GCNGC 5 cut(s) 54, 189, 192, 525, 646
Sau3AI GATC 3 cut(s) 463, 973, 1011
Sau96I GGNCC 2 cut(s) 199, 324
ScaI AGTACT 1 cut(s) 160
SchI GAGTC 2 cut(s) 1057, 1193
ScrFI CCNGG 5 cut(s) 196, 311, 782, 1147, 1166
SduI GDGCHC 2 cut(s) 66, 665
SfaNI GCATC 1 cut(s) 1052
SfcI CTRYAG 2 cut(s) 459, 960
SfuI TTCGAA 1 cut(s) 844
SinI GGWCC 1 cut(s) 199
SmiMI CAYNNNNRTG 2 cut(s) 77, 981
Sse9I AATT 6 cut(s) 34, 216, 229, 294, 836, 1176
SseBI AGGCCT 1 cut(s) 1145
SsiI CCGC 6 cut(s) 141, 447, 525, 611, 957, 1154
SspMI CTAG 2 cut(s) 410, 918
SstI GAGCTC 1 cut(s) 66
StuI AGGCCT 1 cut(s) 1145
StyD4I CCNGG 5 cut(s) 194, 309, 780, 1145, 1164
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 4 cut(s) 137, 641, 687, 1098
TaiI ACGT 2 cut(s) 855, 1092
TaqI TCGA 4 cut(s) 66, 689, 844, 1138
TasI AATT 6 cut(s) 34, 216, 229, 294, 836, 1176
TatI WGTACW 2 cut(s) 158, 993
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 2 cut(s) 303, 694
Tru1I TTAA 2 cut(s) 657, 674
Tru9I TTAA 2 cut(s) 657, 674
TscAI CASTG 3 cut(s) 460, 690, 727
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 4 cut(s) 53, 188, 191, 645
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 4 cut(s) 33, 110, 559, 1048
TspRI CASTG 3 cut(s) 460, 690, 727
VpaK11BI GGWCC 1 cut(s) 199
VspI ATTAAT 1 cut(s) 674
XapI RAATTY 2 cut(s) 229, 836
XceI RCATGY 4 cut(s) 100, 508, 518, 1191
XmiI GTMKAC 2 cut(s) 689, 1086
XspI CTAG 2 cut(s) 410, 918
ZrmI AGTACT 1 cut(s) 160
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.