Rroxscaffold_4G00325150

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001466
Physical Location & Seq
Reverse (-)
56913206 .. 56915100
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325150.1

Sequence Viewer

Length: 1527 bp
ATGGGTAGCCTCCCATCCCACCATGATTCACATGAAACCACCTCTGCCCCCATCACCAAATCCCTAGACCCTGAAGAATTGAGGAAATACGGCCACATGGTCGTAGACTTCATCGCAGATTATTACCAAAACATAGAGAAATACCCAGTTCTAAGCCGAGTGCAACCTGGTTATTTGAGAAAATGCCTATCAGATTCTGCCCCATATGACCCTGAACCCATATCAACCATCCTTAAAGATGTTCAGGATCACATTGTTCCTGGCCTAACACATTGGCAAAGTCCAAATTTCTTTGCATACTTCTCTTCAACTGCTAGCACTGCTGGGTTTCTCGGTGAAATGCTCACCACTGGATTTAATGTGGTGGGGTTCAACTGGGTTTCATCACCAGCAGCAACCGAGCTCGAAAACATAGTCATGGATTGGTTGGCAGACATGCTTCAGCTTCCCAAGTCTTTCCATTTCTCTGGCAACGGAGGCGGTGTGTTACATGGGAGTACTTGTGAGGCCATTGTTTGCACAATGGCAGCTGCCAGGGACCAAATGCTAAGCCGAATTGGTAGCGAGAATTTGGGAAATCTTGTTGTGTATGGTTCAGACCAAACACACAGCACACTTCAGAAAGCTTCCCAAATTCTTGGAATCAACATGGAGAACTTCCGGGCCATCAAGACTACAAAGTCAACTGAATTTTCACTATCACCTGAGATGCTAAGATTGACAATAAGTTCAGACCTAGAAGAAGGGCTAGTGCCACTCTTTGTATGTGCCACAATAGGCACAACCGCAACCACTGCTATTGATCCATTGGAGGCACTATGTCATGTAGCAAAAGAATACGGCGTGTGGGTTCATGTGGATGGTGCATATGCTGGAAGTGCATGCATTTGTCCAGAGTTTCGACATTTCATCGATGGCGTAGAGGGCGCCTACTCTTTTACTTTTAACCCCCATAAGTGGTTATTCACTGGAATGGATTGCTGCTGTCTCTGGGTTAAGAATCCGAGTGCATTGGTACTTTCATTGTCAACGAATCCAGAGTTTTTGAGGAACACAGCCAGTGACTCAAAGAAAGTTGTGGACTACAAAGACTGGCAAATAGCACTGAGCCGGCGGTTCCGAGCATTGAGGGTATGGCTTGTGCTAAGAAGCTACGGCGTGGCCAACCTTCGAAACTTTATTCGTAGCCATGTCAAAATGGCCAAGACTTTTGATGGGCTTGTGAGAATGGACAAGAGGTTTGAGATTGTGGTGCCTAGAGACTTCTCCTTGGTCTGCTTTAGAATTTCACCATCGGCATTGATCAGTAGGGATGAGGATGGTGAGATCGGTATGATAAACGAGGTCAACTGCAAGCTGCTAGATGCCATCAACGAATCAGGTAAAGCATACATGACCCATGCTGTGGTTGGAGGTATCTTTCTGCTGCGGTGCGCCATTGGTGCTACTCTGACAGAAGAAAAGCACATAGTCCAGGCTTGGAAGGTGGTGCAAGACCATGCACAAGCCATCCTCAATATGCATTGA

Protein Analysis

508

Amino Acids

56.38

Weight (kDa)

5.98

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 56 - 429 2.1e-111 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 679
AccB1I GGYRCC 2 cut(s) 926, 1252
AccB7I CCANNNNNTGG 1 cut(s) 1405
AccI GTMKAC 1 cut(s) 105
AciI CCGC 4 cut(s) 480, 786, 1114, 1429
AclWI GGATC 2 cut(s) 255, 797
AcoI YGGCCR 3 cut(s) 91, 1161, 1200
AcsI RAATTY 5 cut(s) 286, 568, 633, 689, 1284
AcuI CTGAAG 3 cut(s) 93, 425, 602
AcyI GRCGYC 1 cut(s) 927
AfaI GTAC 2 cut(s) 499, 1017
AfiI CCNNNNNNNGG 2 cut(s) 957, 1405
AgsI TTSAA 2 cut(s) 309, 373
AjnI CCWGG 4 cut(s) 166, 259, 533, 1473
AluBI AGCT 6 cut(s) 403, 445, 530, 626, 1152, 1357
AluI AGCT 6 cut(s) 403, 445, 530, 626, 1152, 1357
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 2 cut(s) 992, 1254
AlwI GGATC 2 cut(s) 255, 797
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 6 cut(s) 91, 262, 507, 663, 1161, 1200
ApeKI GCWGC 6 cut(s) 392, 527, 530, 981, 1357, 1426
ApoI RAATTY 5 cut(s) 286, 568, 633, 689, 1284
ArsI GACNNNNNNTTYG 4 cut(s) 399, 431, 1223, 1255
AspLEI GCGC 2 cut(s) 929, 1436
AspS9I GGNCC 2 cut(s) 538, 663
AsuC2I CCSGG 1 cut(s) 662
AsuHPI GGTGA 7 cut(s) 46, 337, 347, 378, 693, 1281, 1334
AsuII TTCGAA 1 cut(s) 1171
AsuNHI GCTAGC 1 cut(s) 314
AvaII GGWCC 1 cut(s) 538
BalI TGGCCA 2 cut(s) 1163, 1202
BanI GGYRCC 2 cut(s) 926, 1252
BanII GRGCYC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 6 cut(s) 404, 517, 539, 968, 1344, 1413
BceAI ACGGC 3 cut(s) 106, 856, 1171
BciT130I CCWGG 4 cut(s) 168, 261, 535, 1475
BclI TGATCA 1 cut(s) 1302
BcnI CCSGG 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 992, 1254
BfaI CTAG 6 cut(s) 65, 315, 737, 749, 1257, 1361
BfoI RGCGCY 1 cut(s) 930
BisI GCNGC 6 cut(s) 393, 528, 531, 982, 1358, 1427
BlpI GCTNAGC 1 cut(s) 548
BlsI GCNGC 6 cut(s) 394, 529, 532, 983, 1359, 1428
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 5 cut(s) 168, 261, 535, 662, 1475
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 2 cut(s) 538, 663
BmiI GGNNCC 4 cut(s) 539, 928, 1118, 1254
BmrFI CCNGG 5 cut(s) 168, 261, 535, 662, 1475
BmrI ACTGGG 2 cut(s) 140, 385
BmsI GCATC 2 cut(s) 699, 1354
BmtI GCTAGC 1 cut(s) 318
BmuI ACTGGG 2 cut(s) 140, 385
Bpu1102I GCTNAGC 1 cut(s) 548
Bpu14I TTCGAA 1 cut(s) 1171
BpuMI CCSGG 1 cut(s) 662
Bsa29I ATCGAT 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 927
BsaJI CCNNGG 2 cut(s) 534, 1269
BsaXI ACNNNNNCTCC 4 cut(s) 468, 487, 498, 517
Bsc4I CCNNNNNNNGG 2 cut(s) 957, 1405
Bse118I RCCGGY 1 cut(s) 1110
Bse1I ACTGG 6 cut(s) 146, 355, 380, 973, 1059, 1097
BseBI CCWGG 4 cut(s) 168, 261, 535, 1475
BseCI ATCGAT 1 cut(s) 912
BseDI CCNNGG 2 cut(s) 534, 1269
BseGI GGATG 6 cut(s) 14, 228, 865, 1318, 1324, 1509
BseLI CCNNNNNNNGG 2 cut(s) 957, 1405
BseMII CTCAG 2 cut(s) 696, 1097
BseNI ACTGG 6 cut(s) 146, 355, 380, 973, 1059, 1097
BseXI GCAGC 6 cut(s) 404, 517, 539, 968, 1344, 1413
BseYI CCCAGC 1 cut(s) 323
BshFI GGCC 6 cut(s) 93, 264, 509, 665, 1163, 1202
BshNI GGYRCC 2 cut(s) 926, 1252
BshVI ATCGAT 1 cut(s) 912
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 2 cut(s) 661, 1111
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 957, 1405
BsmAI GTCTC 2 cut(s) 992, 1254
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 6 cut(s) 93, 264, 509, 665, 1163, 1202
Bsp119I TTCGAA 1 cut(s) 1171
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 4 cut(s) 247, 802, 1302, 1326
Bsp1720I GCTNAGC 1 cut(s) 548
BspACI CCGC 4 cut(s) 480, 786, 1114, 1429
BspANI GGCC 6 cut(s) 93, 264, 509, 665, 1163, 1202
BspCNI CTCAG 2 cut(s) 697, 1098
BspDI ATCGAT 1 cut(s) 912
BspLI GGNNCC 4 cut(s) 539, 928, 1118, 1254
BspOI GCTAGC 1 cut(s) 318
BspPI GGATC 2 cut(s) 255, 797
BspT104I TTCGAA 1 cut(s) 1171
BspT107I GGYRCC 2 cut(s) 926, 1252
BsrFI RCCGGY 1 cut(s) 1110
BsrI ACTGG 6 cut(s) 146, 355, 380, 973, 1059, 1097
BssAI RCCGGY 1 cut(s) 1110
BssECI CCNNGG 2 cut(s) 534, 1269
BssMI GATC 4 cut(s) 247, 802, 1302, 1326
BssNI GRCGYC 1 cut(s) 927
BssT1I CCWWGG 1 cut(s) 1269
Bst2UI CCWGG 4 cut(s) 168, 261, 535, 1475
Bst6I CTCTTC 1 cut(s) 310
BstACI GRCGYC 1 cut(s) 927
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 1171
BstC8I GCNNGC 4 cut(s) 316, 883, 1112, 1355
BstDEI CTNAG 6 cut(s) 152, 548, 705, 713, 1106, 1145
BstF5I GGATG 6 cut(s) 14, 228, 865, 1318, 1324, 1509
BstH2I RGCGCY 1 cut(s) 930
BstHHI GCGC 2 cut(s) 929, 1436
BstKTI GATC 4 cut(s) 250, 805, 1305, 1329
BstMAI GTCTC 2 cut(s) 992, 1254
BstMBI GATC 4 cut(s) 247, 802, 1302, 1326
BstMWI GCNNNNNNNGC 6 cut(s) 320, 477, 794, 878, 924, 1442
BstNI CCWGG 4 cut(s) 168, 261, 535, 1475
BstNSI RCATGY 2 cut(s) 439, 885
BstSCI CCNGG 5 cut(s) 166, 259, 533, 660, 1473
BstV1I GCAGC 6 cut(s) 404, 517, 539, 968, 1344, 1413
BstXI CCANNNNNNTGG 2 cut(s) 467, 638
Bsu15I ATCGAT 1 cut(s) 912
BsuRI GGCC 6 cut(s) 93, 264, 509, 665, 1163, 1202
BsuTUI ATCGAT 1 cut(s) 912
BtgZI GCGATG 1 cut(s) 97
BtsCI GGATG 6 cut(s) 14, 228, 865, 1318, 1324, 1509
BtsI GCAGTG 2 cut(s) 318, 792
BtsIMutI CAGTG 6 cut(s) 318, 348, 792, 966, 1066, 1103
Cac8I GCNNGC 4 cut(s) 316, 883, 1112, 1355
CaiI CAGNNNCTG 1 cut(s) 197
CfoI GCGC 2 cut(s) 929, 1436
Cfr10I RCCGGY 1 cut(s) 1110
Cfr13I GGNCC 2 cut(s) 538, 663
ClaI ATCGAT 1 cut(s) 912
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 2 cut(s) 498, 1016
CspCI CAANNNNNGTGG 2 cut(s) 781, 816
CviQI GTAC 2 cut(s) 498, 1016
DdeI CTNAG 6 cut(s) 152, 548, 705, 713, 1106, 1145
DinI GGCGCC 1 cut(s) 928
DpnI GATC 4 cut(s) 249, 804, 1304, 1328
DpnII GATC 4 cut(s) 247, 802, 1302, 1326
DrdI GACNNNNNNGTC 1 cut(s) 679
DseDI GACNNNNNNGTC 1 cut(s) 679
EaeI YGGCCR 3 cut(s) 91, 1161, 1200
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 1269
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 3 cut(s) 93, 425, 602
EcoICRI GAGCTC 1 cut(s) 403
EcoRII CCWGG 4 cut(s) 166, 259, 533, 1473
EcoT14I CCWWGG 1 cut(s) 1269
EcoT22I ATGCAT 2 cut(s) 887, 1524
EcoT38I GRGCYC 1 cut(s) 405
EgeI GGCGCC 1 cut(s) 928
EheI GGCGCC 1 cut(s) 928
ErhI CCWWGG 1 cut(s) 1269
FaqI GGGAC 1 cut(s) 551
FauNDI CATATG 2 cut(s) 205, 868
FbaI TGATCA 1 cut(s) 1302
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 6 cut(s) 393, 528, 531, 982, 1358, 1427
FokI GGATG 5 cut(s) 215, 872, 1325, 1331, 1496
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 6 cut(s) 393, 528, 531, 982, 1358, 1427
FspBI CTAG 6 cut(s) 65, 315, 737, 749, 1257, 1361
GlaI GCGC 2 cut(s) 928, 1435
GluI GCNGC 6 cut(s) 393, 528, 531, 982, 1358, 1427
GsaI CCCAGC 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 930
HaeIII GGCC 6 cut(s) 93, 264, 509, 665, 1163, 1202
HapII CCGG 2 cut(s) 661, 1111
HhaI GCGC 2 cut(s) 929, 1436
Hin1I GRCGYC 1 cut(s) 927
Hin6I GCGC 2 cut(s) 927, 1434
HinP1I GCGC 2 cut(s) 927, 1434
HincII GTYRAC 3 cut(s) 684, 1029, 1348
HindII GTYRAC 3 cut(s) 684, 1029, 1348
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 7 cut(s) 26, 194, 642, 1000, 1033, 1064, 1376
HpaII CCGG 2 cut(s) 661, 1111
HphI GGTGA 7 cut(s) 46, 337, 347, 378, 693, 1281, 1334
Hpy166II GTNNAC 5 cut(s) 106, 684, 1029, 1081, 1348
Hpy188I TCNGA 7 cut(s) 193, 598, 621, 733, 1005, 1121, 1452
Hpy188III TCNNGA 4 cut(s) 245, 670, 893, 1037
Hpy8I GTNNAC 5 cut(s) 106, 684, 1029, 1081, 1348
HpyAV CCTTC 3 cut(s) 737, 1178, 1477
HpyF10VI GCNNNNNNNGC 6 cut(s) 320, 477, 794, 878, 924, 1442
HpyF3I CTNAG 6 cut(s) 152, 548, 705, 713, 1106, 1145
Hsp92I GRCGYC 1 cut(s) 927
HspAI GCGC 2 cut(s) 927, 1434
KasI GGCGCC 1 cut(s) 926
KroI GCCGGC 1 cut(s) 1110
KroNI GCCGGC 1 cut(s) 1112
Ksp22I TGATCA 1 cut(s) 1302
Kzo9I GATC 4 cut(s) 247, 802, 1302, 1326
Lsp1109I GCAGC 6 cut(s) 404, 517, 539, 968, 1344, 1413
LweI GCATC 2 cut(s) 699, 1354
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 6 cut(s) 65, 315, 737, 749, 1257, 1361
MaeIII GTNAC 2 cut(s) 486, 1061
MalI GATC 4 cut(s) 249, 804, 1304, 1328
MboI GATC 4 cut(s) 247, 802, 1302, 1326
MboII GAAGA 4 cut(s) 86, 297, 752, 1469
MhlI GDGCHC 1 cut(s) 405
MlsI TGGCCA 2 cut(s) 1163, 1202
MluCI AATT 7 cut(s) 77, 286, 555, 568, 633, 689, 1284
MluNI TGGCCA 2 cut(s) 1163, 1202
Mly113I GGCGCC 1 cut(s) 927
MlyI GAGTC 1 cut(s) 1058
MmeI TCCRAC 1 cut(s) 1390
Mox20I TGGCCA 2 cut(s) 1163, 1202
Mph1103I ATGCAT 2 cut(s) 887, 1524
MroNI GCCGGC 1 cut(s) 1110
MscI TGGCCA 2 cut(s) 1163, 1202
MseI TTAA 4 cut(s) 234, 357, 945, 996
MslI CAYNNNNRTG 3 cut(s) 416, 858, 971
Msp20I TGGCCA 2 cut(s) 1163, 1202
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 2 cut(s) 661, 1111
MspR9I CCNGG 5 cut(s) 168, 261, 535, 662, 1475
MvaI CCWGG 4 cut(s) 168, 261, 535, 1475
MwoI GCNNNNNNNGC 6 cut(s) 320, 477, 794, 878, 924, 1442
NaeI GCCGGC 1 cut(s) 1112
NarI GGCGCC 1 cut(s) 927
NciI CCSGG 1 cut(s) 662
NdeI CATATG 2 cut(s) 205, 868
NdeII GATC 4 cut(s) 247, 802, 1302, 1326
NgoMIV GCCGGC 1 cut(s) 1110
NheI GCTAGC 1 cut(s) 314
NlaIV GGNNCC 4 cut(s) 539, 928, 1118, 1254
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 1 cut(s) 1061
NsiI ATGCAT 2 cut(s) 887, 1524
NspI RCATGY 2 cut(s) 439, 885
NspV TTCGAA 1 cut(s) 1171
PaeI GCATGC 1 cut(s) 885
PdiI GCCGGC 1 cut(s) 1112
PfeI GAWTC 6 cut(s) 26, 194, 642, 1000, 1033, 1376
PflMI CCANNNNNTGG 1 cut(s) 1405
PkrI GCNGC 6 cut(s) 394, 529, 532, 983, 1359, 1428
PleI GAGTC 1 cut(s) 1058
PluTI GGCGCC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 1058
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 4 cut(s) 166, 259, 533, 1473
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 4 cut(s) 166, 259, 533, 1473
PspN4I GGNNCC 4 cut(s) 539, 928, 1118, 1254
PspPI GGNCC 2 cut(s) 538, 663
PstNI CAGNNNCTG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 2 cut(s) 499, 1017
RsaNI GTAC 2 cut(s) 498, 1016
RseI CAYNNNNRTG 3 cut(s) 416, 858, 971
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 4 cut(s) 234, 357, 945, 996
SatI GCNGC 6 cut(s) 393, 528, 531, 982, 1358, 1427
Sau3AI GATC 4 cut(s) 247, 802, 1302, 1326
Sau96I GGNCC 2 cut(s) 538, 663
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 1 cut(s) 1058
ScrFI CCNGG 5 cut(s) 168, 261, 535, 662, 1475
SduI GDGCHC 1 cut(s) 405
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 2 cut(s) 699, 1354
SfoI GGCGCC 1 cut(s) 928
SfuI TTCGAA 1 cut(s) 1171
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 3 cut(s) 416, 858, 971
SphI GCATGC 1 cut(s) 885
Sse9I AATT 7 cut(s) 77, 286, 555, 568, 633, 689, 1284
SsiI CCGC 4 cut(s) 480, 786, 1114, 1429
SspDI GGCGCC 1 cut(s) 926
SspMI CTAG 6 cut(s) 65, 315, 737, 749, 1257, 1361
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 5 cut(s) 166, 259, 533, 660, 1473
StyI CCWWGG 1 cut(s) 1269
TaqI TCGA 4 cut(s) 405, 901, 912, 1171
TasI AATT 7 cut(s) 77, 286, 555, 568, 633, 689, 1284
TatI WGTACW 1 cut(s) 497
TfiI GAWTC 6 cut(s) 26, 194, 642, 1000, 1033, 1376
Tru1I TTAA 4 cut(s) 234, 357, 945, 996
Tru9I TTAA 4 cut(s) 234, 357, 945, 996
TscAI CASTG 6 cut(s) 325, 355, 799, 973, 1066, 1110
TseFI GTSAC 1 cut(s) 1061
TseI GCWGC 6 cut(s) 392, 527, 530, 981, 1357, 1426
Tsp45I GTSAC 1 cut(s) 1061
TspDTI ATGAA 6 cut(s) 48, 100, 372, 842, 898, 1011
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 6 cut(s) 325, 355, 799, 973, 1066, 1110
Van91I CCANNNNNTGG 1 cut(s) 1405
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 5 cut(s) 286, 568, 633, 689, 1284
XceI RCATGY 2 cut(s) 439, 885
XcmI CCANNNNNNNNNTGG 1 cut(s) 1406
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 6 cut(s) 65, 315, 737, 749, 1257, 1361
ZrmI AGTACT 1 cut(s) 499
Zsp2I ATGCAT 2 cut(s) 887, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.