RchiOBHm_Chr5g0015051

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001466
Physical Location & Seq
Reverse (-)
10430541 .. 10432088
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29548

Sequence Viewer

Length: 1548 bp
ATGGGCAGCCTCCCGTTCCTCCATGATCCACTTGAAAACAGCTCTTCCCACCTGACCAAAGCCCTAGACACTGAAGAGTTCAGGAAACAAGGCCATATGGTCATAGACTTCATTGCAGATTATTACCAAAACATAGAAAAATACCCGGTTCTAAGCCGAGTAGAACCCGGGTATTTGAAAAAATGCCTACCAGAGTCTGCTCCATATAATCCTGAATCGATACAAACCATACTCCAAGATGTTAAAGATCACATTATTCCCGGCCTAACACATTGGCAAAGTCCCAATTTTTTCGCATACTTTTCTTCGACTGCAAGCACTGCTGGGTTTCTTGGCGAAATGCTCATAACTGGGTTCAATGTAGTGGGGTTCAATTGGGTTTCCTCGCCGGCTGCAACCGAGCTCGAACACATGGTCATGGATTGGCTCGCAGACATGCTTCAGCTTCCCAAGTCGTTTCATTTCTCTGGTAACGGTGGCGGTGTGTTACATGGGAGTACTTGCGAAGCCATTGTTTGCACAATGGCAGCTGCCAGGGACCAAATGCTAAGCCGAATTGGTAGCGAGAATTTGGGAAAGCTTGTTGTGTATGGTTCAGACCAAACACATAGCACACTTCAAAAAGCTACCCAAATTGTTGGAATCAACCCGGAGAACTTCAGGGCCATCAAGACTACAAAGTCAACTGGATTTGCACTATCACCTGCAATGCTAAGACTGACAATAAGTTCAGACAGAGCAAAAGGGCTAGTGCCACTTTTTCTATGTGCCACAATGGGCACAACCGCAACCACTGCTATAGATCCATTGGGGGCAGTGTGCAATGTAGCCAAAGATTATGGCATGTGGGTTCATGTGGATGCTGCATATGCAGGAAGTGCTTGCATTTGTCCAGAGTTTCGTCATTTCATTGATGGCGTTGAGGGTGCAAACTCATTTAGTTTCAACCCGCATAAATGGTTATTTACTGCAATGGACTGTTGCTGCCTTTGGGTTGAGAACCCAAGTGCATTAATAAATTCACTGTCGACGAATCTAGAGTATTTGAGGAACAAAGCCAGTGATACAAAGCAAGTTGTGGACTACAAAAACTGGCAAATAGCACTAAGCCGGAGGTTCCGGGCATTGAAGCTATGGCTTGTGCTAAGAAGCTACGGCGTGGCTAACCTTAGAAAATTTATTCGAAGCCATGTCAAAATAGCCAAGATTTTTGAAGGGCTTGTGAGAATGGACAATAGGTTTGAAATTGTGGTTCCTAGAAACTTCTCCTTGGTCTGTTTCAGGATTTCACTATCCGCAATAGTTAGTGATGATCATTATGATGGTGAGGAGTACAATATGGTAAACGAGATCAATTGCAAGTTGCTGGAGGCCATCAATGCATCAGGCTCTGTGTTCATGACTCATGCTGTGGTTGGAGGTGTCTACGTGCTACGGTGCGCAGTTGGTGCAACTCTAACTGAGGAAAAGCACATTCGAGAGGCCTGGAAGGCGGTGCAAGGCCAGGCACATACAATTCTCTCAGCATGTATTGTTGACTCTGCATAA

Protein Analysis

515

Amino Acids

57.0

Weight (kDa)

6.49

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 56 - 429 6.7e-113 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 712
AasI GACNNNNNNGTC 1 cut(s) 679
Acc16I TGCGCA 1 cut(s) 1441
Acc36I ACCTGC 1 cut(s) 712
AccI GTMKAC 2 cut(s) 1028, 1425
AciI CCGC 5 cut(s) 480, 786, 950, 1296, 1493
AclWI GGATC 2 cut(s) 20, 797
AcsI RAATTY 3 cut(s) 568, 1018, 1175
AcuI CTGAAG 3 cut(s) 93, 425, 643
AfaI GTAC 2 cut(s) 499, 1334
AgsI TTSAA 9 cut(s) 35, 178, 358, 373, 620, 946, 1129, 1214, 1244
AjnI CCWGG 3 cut(s) 533, 1484, 1503
AluBI AGCT 8 cut(s) 42, 403, 445, 530, 580, 626, 1132, 1152
AluI AGCT 8 cut(s) 42, 403, 445, 530, 580, 626, 1132, 1152
Alw21I GWGCWC 1 cut(s) 405
AlwI GGATC 2 cut(s) 20, 797
AlwNI CAGNNNCTG 2 cut(s) 197, 1391
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 6 cut(s) 91, 262, 663, 1371, 1482, 1501
ApeKI GCWGC 6 cut(s) 6, 392, 527, 530, 863, 984
ApoI RAATTY 3 cut(s) 568, 1018, 1175
ArsI GACNNNNNNTTYG 4 cut(s) 399, 431, 1223, 1255
AseI ATTAAT 1 cut(s) 1013
AspLEI GCGC 1 cut(s) 1442
AspS9I GGNCC 2 cut(s) 538, 663
AsuC2I CCSGG 6 cut(s) 146, 168, 169, 261, 650, 1121
AsuHPI GGTGA 2 cut(s) 693, 1337
AsuII TTCGAA 1 cut(s) 1183
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 6 cut(s) 18, 379, 517, 539, 850, 971
BccI CCATC 4 cut(s) 674, 908, 1316, 1382
BceAI ACGGC 1 cut(s) 1171
BciT130I CCWGG 3 cut(s) 535, 1486, 1505
BclI TGATCA 1 cut(s) 1312
BcnI CCSGG 6 cut(s) 146, 168, 169, 261, 650, 1121
BfaI CTAG 4 cut(s) 65, 749, 1037, 1257
BfmI CTRYAG 1 cut(s) 798
BfuAI ACCTGC 1 cut(s) 712
BglI GCCNNNNNGGC 1 cut(s) 1490
BisI GCNGC 6 cut(s) 7, 393, 528, 531, 864, 985
BlpI GCTNAGC 1 cut(s) 548
BlsI GCNGC 6 cut(s) 8, 394, 529, 532, 865, 986
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 9 cut(s) 146, 168, 169, 261, 535, 650, 1121, 1486, 1505
Bme18I GGWCC 1 cut(s) 538
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 538, 663
BmiI GGNNCC 3 cut(s) 539, 1118, 1254
BmrFI CCNGG 9 cut(s) 146, 168, 169, 261, 535, 650, 1121, 1486, 1505
BmrI ACTGGG 1 cut(s) 360
BmsI GCATC 2 cut(s) 850, 1391
BmuI ACTGGG 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 1388
Bpu1102I GCTNAGC 1 cut(s) 548
Bpu14I TTCGAA 1 cut(s) 1183
BpuMI CCSGG 6 cut(s) 146, 168, 169, 261, 650, 1121
Bsa29I ATCGAT 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 1429
BsaJI CCNNGG 3 cut(s) 167, 534, 1269
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 4 cut(s) 355, 691, 1059, 1097
Bse3DI GCAATG 4 cut(s) 111, 714, 829, 978
BseBI CCWGG 3 cut(s) 535, 1486, 1505
BseCI ATCGAT 1 cut(s) 218
BseDI CCNNGG 3 cut(s) 167, 534, 1269
BseGI GGATG 1 cut(s) 865
BseMI GCAATG 4 cut(s) 111, 714, 829, 978
BseMII CTCAG 2 cut(s) 1452, 1536
BseNI ACTGG 4 cut(s) 355, 691, 1059, 1097
BseRI GAGGAG 1 cut(s) 1343
BseSI GKGCMC 1 cut(s) 782
BseXI GCAGC 6 cut(s) 18, 379, 517, 539, 850, 971
BseYI CCCAGC 1 cut(s) 323
BshFI GGCC 6 cut(s) 93, 264, 665, 1373, 1484, 1503
BshVI ATCGAT 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 7 cut(s) 146, 168, 261, 389, 650, 1111, 1120
BslFI GGGAC 2 cut(s) 267, 551
BsmFI GGGAC 2 cut(s) 267, 551
BsnI GGCC 6 cut(s) 93, 264, 665, 1373, 1484, 1503
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 1183
Bsp1286I GDGCHC 2 cut(s) 405, 782
Bsp143I GATC 5 cut(s) 25, 247, 802, 1312, 1350
Bsp1720I GCTNAGC 1 cut(s) 548
BspACI CCGC 5 cut(s) 480, 786, 950, 1296, 1493
BspANI GGCC 6 cut(s) 93, 264, 665, 1373, 1484, 1503
BspCNI CTCAG 2 cut(s) 1453, 1535
BspDI ATCGAT 1 cut(s) 218
BspHI TCATGA 1 cut(s) 1398
BspLI GGNNCC 3 cut(s) 539, 1118, 1254
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 2 cut(s) 20, 797
BspQI GCTCTTC 1 cut(s) 49
BspT104I TTCGAA 1 cut(s) 1183
BsrDI GCAATG 4 cut(s) 111, 714, 829, 978
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 4 cut(s) 355, 691, 1059, 1097
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 167, 534, 1269
BssMI GATC 5 cut(s) 25, 247, 802, 1312, 1350
BssT1I CCWWGG 1 cut(s) 1269
Bst2UI CCWGG 3 cut(s) 535, 1486, 1505
Bst4CI ACNGT 4 cut(s) 476, 980, 1026, 1437
Bst6I CTCTTC 2 cut(s) 49, 69
BstAPI GCANNNNNTGC 4 cut(s) 320, 794, 878, 1448
BstBAI YACGTR 1 cut(s) 1429
BstBI TTCGAA 1 cut(s) 1183
BstC8I GCNNGC 4 cut(s) 316, 390, 429, 883
BstDEI CTNAG 8 cut(s) 152, 548, 713, 1106, 1145, 1169, 1461, 1522
BstF5I GGATG 1 cut(s) 865
BstHHI GCGC 1 cut(s) 1442
BstKTI GATC 5 cut(s) 28, 250, 805, 1315, 1353
BstMBI GATC 5 cut(s) 25, 247, 802, 1312, 1350
BstMWI GCNNNNNNNGC 7 cut(s) 320, 794, 869, 878, 1379, 1448, 1490
BstNI CCWGG 3 cut(s) 535, 1486, 1505
BstNSI RCATGY 3 cut(s) 439, 847, 1530
BstSCI CCNGG 9 cut(s) 144, 166, 167, 259, 533, 648, 1119, 1484, 1503
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 782
BstV1I GCAGC 6 cut(s) 18, 379, 517, 539, 850, 971
BstX2I RGATCY 1 cut(s) 802
BstXI CCANNNNNNTGG 1 cut(s) 638
BstYI RGATCY 1 cut(s) 802
Bsu15I ATCGAT 1 cut(s) 218
BsuRI GGCC 6 cut(s) 93, 264, 665, 1373, 1484, 1503
BsuTUI ATCGAT 1 cut(s) 218
BtsCI GGATG 1 cut(s) 865
BtsI GCAGTG 3 cut(s) 318, 792, 822
BtsIMutI CAGTG 6 cut(s) 69, 318, 792, 822, 1022, 1066
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 4 cut(s) 316, 390, 429, 883
CaiI CAGNNNCTG 2 cut(s) 197, 1391
CciI TCATGA 1 cut(s) 1398
CfoI GCGC 1 cut(s) 1442
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 2 cut(s) 538, 663
Cfr9I CCCGGG 1 cut(s) 167
ClaI ATCGAT 1 cut(s) 218
Csp6I GTAC 2 cut(s) 498, 1333
CviQI GTAC 2 cut(s) 498, 1333
DdeI CTNAG 8 cut(s) 152, 548, 713, 1106, 1145, 1169, 1461, 1522
DpnI GATC 5 cut(s) 27, 249, 804, 1314, 1352
DpnII GATC 5 cut(s) 25, 247, 802, 1312, 1350
DrdI GACNNNNNNGTC 1 cut(s) 679
DseDI GACNNNNNNGTC 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 49, 69
EarI CTCTTC 2 cut(s) 49, 69
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 1269
Eco147I AGGCCT 1 cut(s) 1484
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 3 cut(s) 93, 425, 643
Eco88I CYCGRG 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 403
EcoRII CCWGG 3 cut(s) 533, 1484, 1503
EcoT14I CCWWGG 1 cut(s) 1269
EcoT22I ATGCAT 1 cut(s) 1384
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 1269
FaqI GGGAC 2 cut(s) 267, 551
FauI CCCGC 1 cut(s) 957
FauNDI CATATG 2 cut(s) 96, 868
FbaI TGATCA 1 cut(s) 1312
FblI GTMKAC 2 cut(s) 1028, 1425
Fnu4HI GCNGC 6 cut(s) 7, 393, 528, 531, 864, 985
FokI GGATG 1 cut(s) 872
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 6 cut(s) 7, 393, 528, 531, 864, 985
FspBI CTAG 4 cut(s) 65, 749, 1037, 1257
FspI TGCGCA 1 cut(s) 1441
GlaI GCGC 1 cut(s) 1441
GluI GCNGC 6 cut(s) 7, 393, 528, 531, 864, 985
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 1 cut(s) 1388
HaeIII GGCC 6 cut(s) 93, 264, 665, 1373, 1484, 1503
HapII CCGG 7 cut(s) 146, 168, 261, 389, 650, 1111, 1120
HhaI GCGC 1 cut(s) 1442
Hin6I GCGC 1 cut(s) 1440
HinP1I GCGC 1 cut(s) 1440
HincII GTYRAC 3 cut(s) 684, 1029, 1537
HindII GTYRAC 3 cut(s) 684, 1029, 1537
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 6 cut(s) 194, 215, 642, 1033, 1402, 1538
HpaII CCGG 7 cut(s) 146, 168, 261, 389, 650, 1111, 1120
HphI GGTGA 2 cut(s) 693, 1337
Hpy166II GTNNAC 6 cut(s) 684, 1029, 1081, 1345, 1426, 1537
Hpy188I TCNGA 2 cut(s) 598, 733
Hpy188III TCNNGA 8 cut(s) 82, 212, 670, 893, 1037, 1282, 1399, 1478
Hpy8I GTNNAC 6 cut(s) 684, 1029, 1081, 1345, 1426, 1537
Hpy99I CGWCG 1 cut(s) 1033
HpyAV CCTTC 2 cut(s) 1208, 1483
HpyCH4III ACNGT 4 cut(s) 476, 980, 1026, 1437
HpyCH4IV ACGT 1 cut(s) 1428
HpyF10VI GCNNNNNNNGC 7 cut(s) 320, 794, 869, 878, 1379, 1448, 1490
HpyF3I CTNAG 8 cut(s) 152, 548, 713, 1106, 1145, 1169, 1461, 1522
HpySE526I ACGT 1 cut(s) 1428
HspAI GCGC 1 cut(s) 1440
KroI GCCGGC 1 cut(s) 388
KroNI GCCGGC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 1312
Kzo9I GATC 5 cut(s) 25, 247, 802, 1312, 1350
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 6 cut(s) 18, 379, 517, 539, 850, 971
LweI GCATC 2 cut(s) 850, 1391
MaeI CTAG 4 cut(s) 65, 749, 1037, 1257
MaeII ACGT 1 cut(s) 1428
MaeIII GTNAC 2 cut(s) 470, 486
MalI GATC 5 cut(s) 27, 249, 804, 1314, 1352
MboI GATC 5 cut(s) 25, 247, 802, 1312, 1350
MboII GAAGA 3 cut(s) 36, 86, 297
MfeI CAATTG 2 cut(s) 373, 1354
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 2 cut(s) 405, 782
MlyI GAGTC 3 cut(s) 203, 1396, 1532
MmeI TCCRAC 2 cut(s) 619, 1396
Mph1103I ATGCAT 1 cut(s) 1384
MroNI GCCGGC 1 cut(s) 388
MseI TTAA 2 cut(s) 243, 1013
MslI CAYNNNNRTG 3 cut(s) 416, 858, 1320
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 7 cut(s) 146, 168, 261, 389, 650, 1111, 1120
MspR9I CCNGG 9 cut(s) 146, 168, 169, 261, 535, 650, 1121, 1486, 1505
MunI CAATTG 2 cut(s) 373, 1354
MvaI CCWGG 3 cut(s) 535, 1486, 1505
MwoI GCNNNNNNNGC 7 cut(s) 320, 794, 869, 878, 1379, 1448, 1490
NaeI GCCGGC 1 cut(s) 390
NciI CCSGG 6 cut(s) 146, 168, 169, 261, 650, 1121
NdeI CATATG 2 cut(s) 96, 868
NdeII GATC 5 cut(s) 25, 247, 802, 1312, 1350
NgoMIV GCCGGC 1 cut(s) 388
NlaIV GGNNCC 3 cut(s) 539, 1118, 1254
NmeAIII GCCGAG 1 cut(s) 182
NsbI TGCGCA 1 cut(s) 1441
NsiI ATGCAT 1 cut(s) 1384
NspI RCATGY 3 cut(s) 439, 847, 1530
NspV TTCGAA 1 cut(s) 1183
PagI TCATGA 1 cut(s) 1398
PaqCI CACCTGC 1 cut(s) 712
PceI AGGCCT 1 cut(s) 1484
PciSI GCTCTTC 1 cut(s) 49
PdiI GCCGGC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 215, 642, 1033
PkrI GCNGC 6 cut(s) 8, 394, 529, 532, 865, 986
PleI GAGTC 3 cut(s) 202, 1396, 1532
PpsI GAGTC 3 cut(s) 202, 1396, 1532
Ppu21I YACGTR 1 cut(s) 1429
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 3 cut(s) 533, 1484, 1503
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 3 cut(s) 533, 1484, 1503
PspN4I GGNNCC 3 cut(s) 539, 1118, 1254
PspPI GGNCC 2 cut(s) 538, 663
PstNI CAGNNNCTG 2 cut(s) 197, 1391
PsuI RGATCY 1 cut(s) 802
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 2 cut(s) 499, 1334
RsaNI GTAC 2 cut(s) 498, 1333
RseI CAYNNNNRTG 3 cut(s) 416, 858, 1320
SacI GAGCTC 1 cut(s) 405
SalI GTCGAC 1 cut(s) 1027
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 243, 1013
SatI GCNGC 6 cut(s) 7, 393, 528, 531, 864, 985
Sau3AI GATC 5 cut(s) 25, 247, 802, 1312, 1350
Sau96I GGNCC 2 cut(s) 538, 663
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 3 cut(s) 203, 1396, 1532
ScrFI CCNGG 9 cut(s) 146, 168, 169, 261, 535, 650, 1121, 1486, 1505
SduI GDGCHC 2 cut(s) 405, 782
SfaNI GCATC 2 cut(s) 850, 1391
SfcI CTRYAG 1 cut(s) 798
SfuI TTCGAA 1 cut(s) 1183
SinI GGWCC 1 cut(s) 538
SmaI CCCGGG 1 cut(s) 169
SmiMI CAYNNNNRTG 3 cut(s) 416, 858, 1320
SseBI AGGCCT 1 cut(s) 1484
SsiI CCGC 5 cut(s) 480, 786, 950, 1296, 1493
SspMI CTAG 4 cut(s) 65, 749, 1037, 1257
SstI GAGCTC 1 cut(s) 405
StuI AGGCCT 1 cut(s) 1484
StyD4I CCNGG 9 cut(s) 144, 166, 167, 259, 533, 648, 1119, 1484, 1503
StyI CCWWGG 1 cut(s) 1269
TaaI ACNGT 4 cut(s) 476, 980, 1026, 1437
TaiI ACGT 1 cut(s) 1431
TaqI TCGA 6 cut(s) 218, 308, 405, 1028, 1183, 1477
TatI WGTACW 2 cut(s) 497, 1332
TfiI GAWTC 3 cut(s) 215, 642, 1033
Tru1I TTAA 2 cut(s) 243, 1013
Tru9I TTAA 2 cut(s) 243, 1013
TscAI CASTG 6 cut(s) 76, 325, 799, 822, 1029, 1066
TseI GCWGC 6 cut(s) 6, 392, 527, 530, 863, 984
TspDTI ATGAA 5 cut(s) 100, 449, 842, 898, 1387
TspMI CCCGGG 1 cut(s) 167
TspRI CASTG 6 cut(s) 76, 325, 799, 822, 1029, 1066
VpaK11BI GGWCC 1 cut(s) 538
VspI ATTAAT 1 cut(s) 1013
XapI RAATTY 3 cut(s) 568, 1018, 1175
XbaI TCTAGA 1 cut(s) 1036
XceI RCATGY 3 cut(s) 439, 847, 1530
XmaI CCCGGG 1 cut(s) 167
XmiI GTMKAC 2 cut(s) 1028, 1425
XspI CTAG 4 cut(s) 65, 749, 1037, 1257
ZrmI AGTACT 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.