Rorug05G0523300

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001466
Physical Location & Seq
Reverse (-)
70905753 .. 70908576
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0523300.1

Sequence Viewer

Length: 1134 bp
ATGGCGCACGAACCGACAGAACTCCCAAACCTTCCGATGGAGACCATTCGCGAGATTCTCACCTGGCTTCCGGTGAAGCCGTTATGCAGATTCAGGTGCGTATCGAAGTCATGGAATTCCCTAACTTTTGATCCCAAATTTGTGAAAATGCACTTTAACAAAGCCCTTGAGCACGACGATGTACTCTACCAAAGACGAAGAGTCATGGTCAGCGACTGTAATTCCGGCAAGTTTTACTCTTTTAACTTAGATGAGTTTCTCAATCATGATCATATTGATAATGTTGGTGGTGGTGGTGGTGAGAATCATGAATTGTTAGTCACTGAAGTCGATAGAGTCGGAGATGTTTCGGGTTGCTATGTTTTTTACTCCAATGCCTTGTTGTTGTTCCAGTCAAATGAGAGGTTTTTCTTGTTTAACCCGGTAACCAGGGAATCCAAGGTAGTACCTGAGTTTCCAAGACTTGGTCCATGGAGTAGAAGTTTTGGGAAGCTATACGGATTTGGCTTTGATCCATCCACCCAAGATTACAAGATGGTTTATGGGATTAGTGGGGTTTGCTCTACAACCGAACTTAAGTTTAGTGTCTATTCATTGAAAACCGGTTCTTGGCGAGTGATTGAGAAGGGATATCCCTATCAAAGTTTCCACCATGGTATGCAGGGAAGACTGCTGAATGGAGGTATTCATTGGTTGGTTTATAGAGACTATAGAGATGCATCATCTATGGTTCTGTTATCTTTTGTTTTAGCAGAGGAGGAAGTGCGGGAGATTCCACTTCCGCCTTGTTTCAGTATCGAGGGGGAAGATGTTTATTTAAGTGTCTTCAGAGAATGCCTGTGTATGATACTGGGGTATAATGAGGAGCTTTGGGTCATGAAGGAATATGGAGTGAGAGAGTCTTGGACTAAAATAAGGATCTCCATCCCGCCTTGTCAGTGGTTACACTCGGGGTTCAGGAAGAAAAATTATGATCTACTGCTGTTGGAAGATGCCGAAGACAAGTTGGTTCTGCTCAATTTTGATGAGGACACCTTTCGCAATCTATCAATTCGTGGAGTTTCGCAAGTTGGTTATGTTGCGGTCTACTTGGAGAGCCTTGTTTCACCCAATTACTTTGGCGTCACTGATTGA

Protein Analysis

377

Amino Acids

43.66

Weight (kDa)

5.56

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 8 - 47 4.6e-10 F-box domain
F-box-like PF12937 9 - 47 1.6e-07 F-box-like
FBA_1 PF07734 110 - 368 3.4e-22 F-box associated beta propeller domain
FBA_3 PF08268 121 - 317 2.5e-15 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 464
AccI GTMKAC 1 cut(s) 1086
AccII CGCG 1 cut(s) 51
AciI CCGC 4 cut(s) 766, 782, 929, 1082
AclWI GGATC 3 cut(s) 125, 506, 926
AcsI RAATTY 2 cut(s) 115, 137
AcuI CTGAAG 2 cut(s) 345, 811
AcyI GRCGYC 1 cut(s) 1122
AfaI GTAC 2 cut(s) 183, 447
AfiI CCNNNNNNNGG 3 cut(s) 37, 464, 609
AflII CTTAAG 1 cut(s) 575
AgeI ACCGGT 1 cut(s) 602
AgsI TTSAA 1 cut(s) 598
AhdI GACNNNNNGTC 1 cut(s) 200
AjnI CCWGG 2 cut(s) 62, 428
AluBI AGCT 2 cut(s) 493, 868
AluI AGCT 2 cut(s) 493, 868
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 2 cut(s) 35, 699
AlwI GGATC 3 cut(s) 125, 506, 926
AlwNI CAGNNNCTG 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 949
ApoI RAATTY 2 cut(s) 115, 137
AsiGI ACCGGT 1 cut(s) 602
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 467
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 4 cut(s) 52, 85, 311, 1098
AvaI CYCGRG 1 cut(s) 949
AvaII GGWCC 1 cut(s) 467
BaeI ACNNNNGTAYC 2 cut(s) 839, 872
BbsI GAAGAC 3 cut(s) 673, 817, 1005
Bbv12I GWGCWC 1 cut(s) 174
BccI CCATC 4 cut(s) 31, 523, 529, 932
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 2 cut(s) 64, 430
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 35, 699
BfmI CTRYAG 1 cut(s) 709
BfrI CTTAAG 1 cut(s) 575
Bme1390I CCNGG 3 cut(s) 64, 422, 430
Bme18I GGWCC 1 cut(s) 467
BmeRI GACNNNNNGTC 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 949
BmgT120I GGNCC 1 cut(s) 467
BmrFI CCNGG 3 cut(s) 64, 422, 430
BmrI ACTGGG 1 cut(s) 860
BmsI GCATC 3 cut(s) 706, 728, 982
BmuI ACTGGG 1 cut(s) 860
BpiI GAAGAC 3 cut(s) 673, 817, 1005
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 1122
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 4 cut(s) 429, 438, 470, 652
BsaWI WCCGGW 2 cut(s) 70, 602
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 464, 609
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 2 cut(s) 391, 855
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 2 cut(s) 64, 430
BseDI CCNNGG 4 cut(s) 429, 438, 470, 652
BseGI GGATG 2 cut(s) 515, 924
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 3 cut(s) 37, 464, 609
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 2 cut(s) 391, 855
BseRI GAGGAG 2 cut(s) 770, 878
Bsh1236I CGCG 1 cut(s) 51
BshTI ACCGGT 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 949
BsiSI CCGG 4 cut(s) 71, 225, 422, 603
BslI CCNNNNNNNGG 3 cut(s) 37, 464, 609
BsmAI GTCTC 2 cut(s) 35, 699
BsmI GAATGC 1 cut(s) 839
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 949
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 5 cut(s) 130, 268, 511, 918, 973
Bsp19I CCATGG 2 cut(s) 470, 652
Bsp68I TCGCGA 1 cut(s) 51
BspACI CCGC 4 cut(s) 766, 782, 929, 1082
BspCNI CTCAG 1 cut(s) 442
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 3 cut(s) 265, 307, 876
BspPI GGATC 3 cut(s) 125, 506, 926
BspTI CTTAAG 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 2 cut(s) 391, 855
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 4 cut(s) 429, 438, 470, 652
BssMI GATC 5 cut(s) 130, 268, 511, 918, 973
BssNI GRCGYC 1 cut(s) 1122
BssT1I CCWWGG 3 cut(s) 438, 470, 652
Bst2UI CCWGG 2 cut(s) 64, 430
Bst4CI ACNGT 1 cut(s) 218
Bst6I CTCTTC 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 1122
BstAFI CTTAAG 1 cut(s) 575
BstDEI CTNAG 2 cut(s) 247, 450
BstDSI CCRYGG 2 cut(s) 470, 652
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 2 cut(s) 515, 924
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 133, 271, 514, 921, 976
BstMAI GTCTC 2 cut(s) 35, 699
BstMBI GATC 5 cut(s) 130, 268, 511, 918, 973
BstNI CCWGG 2 cut(s) 64, 430
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 62, 420, 428
BstSFI CTRYAG 1 cut(s) 709
BstUI CGCG 1 cut(s) 51
BstV2I GAAGAC 3 cut(s) 673, 817, 1005
BstX2I RGATCY 1 cut(s) 918
BstYI RGATCY 1 cut(s) 918
BtgI CCRYGG 2 cut(s) 470, 652
BtsCI GGATG 2 cut(s) 515, 924
BtsIMutI CAGTG 3 cut(s) 321, 944, 1125
BtuMI TCGCGA 1 cut(s) 51
CaiI CAGNNNCTG 1 cut(s) 216
CciI TCATGA 3 cut(s) 265, 307, 876
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 1 cut(s) 467
CseI GACGC 1 cut(s) 1111
Csp6I GTAC 2 cut(s) 182, 446
CspAI ACCGGT 1 cut(s) 602
CviAII CATG 7 cut(s) 111, 205, 266, 308, 471, 653, 877
CviJI RGCY 7 cut(s) 67, 79, 164, 493, 507, 868, 1098
CviKI_1 RGCY 7 cut(s) 67, 79, 164, 493, 507, 868, 1098
CviQI GTAC 2 cut(s) 182, 446
DdeI CTNAG 2 cut(s) 247, 450
DpnI GATC 5 cut(s) 132, 270, 513, 920, 975
DpnII GATC 5 cut(s) 130, 268, 511, 918, 973
DriI GACNNNNNGTC 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 193
Eam1105I GACNNNNNGTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 193
EciI GGCGGA 1 cut(s) 771
Eco130I CCWWGG 3 cut(s) 438, 470, 652
Eco31I GGTCTC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 467
Eco57I CTGAAG 2 cut(s) 345, 811
Eco88I CYCGRG 1 cut(s) 949
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 62, 428
EcoRV GATATC 1 cut(s) 632
EcoT14I CCWWGG 3 cut(s) 438, 470, 652
EcoT22I ATGCAT 1 cut(s) 721
ErhI CCWWGG 3 cut(s) 438, 470, 652
FaeI CATG 7 cut(s) 114, 208, 269, 311, 474, 656, 880
FatI CATG 7 cut(s) 110, 204, 265, 307, 470, 652, 876
FauI CCCGC 2 cut(s) 759, 936
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 1086
FokI GGATG 2 cut(s) 502, 911
GlaI GCGC 1 cut(s) 6
HapII CCGG 4 cut(s) 71, 225, 422, 603
HgaI GACGC 1 cut(s) 1111
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 1122
Hin1II CATG 7 cut(s) 114, 208, 269, 311, 474, 656, 880
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 8 cut(s) 55, 90, 201, 304, 336, 434, 772, 899
HpaII CCGG 4 cut(s) 71, 225, 422, 603
HphI GGTGA 4 cut(s) 52, 85, 311, 1098
Hpy166II GTNNAC 1 cut(s) 1087
Hpy188I TCNGA 3 cut(s) 36, 341, 830
Hpy188III TCNNGA 5 cut(s) 50, 266, 308, 877, 958
Hpy8I GTNNAC 1 cut(s) 1087
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 41, 619, 874
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4V TGCA 4 cut(s) 87, 151, 661, 719
HpyF3I CTNAG 2 cut(s) 247, 450
Hsp92I GRCGYC 1 cut(s) 1122
Hsp92II CATG 7 cut(s) 114, 208, 269, 311, 474, 656, 880
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 5 cut(s) 130, 268, 511, 918, 973
LmnI GCTCC 1 cut(s) 865
LweI GCATC 3 cut(s) 706, 728, 982
MaeIII GTNAC 4 cut(s) 319, 424, 942, 1123
MalI GATC 5 cut(s) 132, 270, 513, 920, 975
MboI GATC 5 cut(s) 130, 268, 511, 918, 973
MboII GAAGA 7 cut(s) 210, 678, 817, 818, 973, 1001, 1010
MflI RGATCY 1 cut(s) 918
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 8 cut(s) 115, 137, 220, 311, 967, 1018, 1050, 1111
MlyI GAGTC 3 cut(s) 210, 345, 908
MmeI TCCRAC 2 cut(s) 319, 966
MnlI CCTC 7 cut(s) 396, 674, 748, 751, 793, 856, 1021
Mph1103I ATGCAT 1 cut(s) 721
MseI TTAA 5 cut(s) 156, 243, 417, 576, 818
MslI CAYNNNNRTG 1 cut(s) 177
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 4 cut(s) 71, 225, 422, 603
MspR9I CCNGG 3 cut(s) 64, 422, 430
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 2 cut(s) 64, 430
MvnI CGCG 1 cut(s) 51
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 2 cut(s) 470, 652
NdeII GATC 5 cut(s) 130, 268, 511, 918, 973
NlaIII CATG 7 cut(s) 114, 208, 269, 311, 474, 656, 880
NmuCI GTSAC 2 cut(s) 319, 1123
NruI TCGCGA 1 cut(s) 51
NsiI ATGCAT 1 cut(s) 721
PagI TCATGA 3 cut(s) 265, 307, 876
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 5 cut(s) 55, 90, 304, 434, 772
PflFI GACNNNGTC 1 cut(s) 465
PflMI CCANNNNNTGG 1 cut(s) 464
PinAI ACCGGT 1 cut(s) 602
PleI GAGTC 3 cut(s) 209, 344, 907
PpsI GAGTC 3 cut(s) 209, 344, 907
Psp6I CCWGG 2 cut(s) 62, 428
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 62, 428
PspPI GGNCC 1 cut(s) 467
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 1 cut(s) 918
PsyI GACNNNGTC 1 cut(s) 465
RruI TCGCGA 1 cut(s) 51
RsaI GTAC 2 cut(s) 183, 447
RsaNI GTAC 2 cut(s) 182, 446
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 5 cut(s) 156, 243, 417, 576, 818
Sau3AI GATC 5 cut(s) 130, 268, 511, 918, 973
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 3 cut(s) 210, 345, 908
ScrFI CCNGG 3 cut(s) 64, 422, 430
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 3 cut(s) 706, 728, 982
SfcI CTRYAG 1 cut(s) 709
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 2 cut(s) 167, 575
SmoI CTYRAG 2 cut(s) 167, 575
Sse9I AATT 8 cut(s) 115, 137, 220, 311, 967, 1018, 1050, 1111
SsiI CCGC 4 cut(s) 766, 782, 929, 1082
StyD4I CCNGG 3 cut(s) 62, 420, 428
StyI CCWWGG 3 cut(s) 438, 470, 652
TaaI ACNGT 1 cut(s) 218
TaqI TCGA 3 cut(s) 104, 330, 798
TasI AATT 8 cut(s) 115, 137, 220, 311, 967, 1018, 1050, 1111
TatI WGTACW 1 cut(s) 181
TfiI GAWTC 5 cut(s) 55, 90, 304, 434, 772
Tru1I TTAA 5 cut(s) 156, 243, 417, 576, 818
Tru9I TTAA 5 cut(s) 156, 243, 417, 576, 818
TscAI CASTG 3 cut(s) 328, 944, 1132
TseFI GTSAC 2 cut(s) 319, 1123
Tsp45I GTSAC 2 cut(s) 319, 1123
TspDTI ATGAA 4 cut(s) 324, 582, 677, 893
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 3 cut(s) 328, 944, 1132
Tth111I GACNNNGTC 1 cut(s) 465
Van91I CCANNNNNTGG 1 cut(s) 464
Vha464I CTTAAG 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 2 cut(s) 115, 137
XmiI GTMKAC 1 cut(s) 1086
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.