Rw1G005430

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001466
Physical Location & Seq
Forward (+)
10482494 .. 10484400
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005430.1

Sequence Viewer

Length: 1533 bp
ATGGGTAGCCGTAGCCTACCATTCCACCATGATTCACATGAAACCACCTCTGCCCCCATCACCAAATCCCTAGACCCTGAAGAATTGAGGAAATACGGCCACATGGTCGTAGACTTCATCGCAGATTATTACCAAAACATAGAGAAATACCCAGTTCTAAGCCGAGTGCAACCTGGTTATTTGAGAAACTGCCTACCAGCTTCTGCCCCATATGACCCTGAACCCATATCAACCATCCTTAAAGATGTTCAGGATCACATTGTTCCTGGCCTAACACATTGGCAAAGTCCAAATTTCTTTGCATACTTCTCTTCAACTGCTAGCACTGCAGGGTTTCTCGGTGAAATGCTCACCACTGGACTTAATGTGGTGGGGTTCAACTGGGTTTCATCACCAGCAGCAACCGAGCTCGAAAACATAGTCATGGATTGGTTGGCAGACATGCTTCAGCTTCCCAAGTCTTTCCATTTCTCTGGCAACGGAGGCGGTGTGTTACATGGGAGTACTTGCGAGGCCATTGTTTGCACAATGGCAGCTGCCAGAGACCAAATGCTAAGCCGAATTGGTAGCGAGAATTTGGGAAATCTTGTTGTGTATGGTTCAGACCAAACACATAGCACACTTCAGAAAGCTTCCCAAATTCTTGGAATCAACATGGAGAACTTCCGGGCCATCAAGACTACAAAGTCAACTGAATTTGCACTATCACCTGAGATGCTAAGATTGACAATAAGTTCAGACCTAGAAGAAGGGCTAGTGCCACTCTTTGTATGTGCCACAATAGGCACAACCGCAACCACTGCTATTGATCCATTGGAGGCACTATGTCATGTAGCAAAAGAATACGGCGTGTGGGTTCATGTGGATGGTGCATATGCTGGAAGTGCATGCATTTGTCCAGAGTTTCGACATTTCATCGATGGCGTAGAGGGCGCAAACTCTTTTAGTTTTAACCCCCATAAATGGTTATTCACTGGAATGGATTGCTGCTGCCTTTGGGTTAAGAATCCGAGTGCATTGGCACTTTCATTGTCAACGAATCCAGAGTTTTTGAGGAACACAGCCAGTGACTCAAAGCAAGTTGTGGACTACAAAGACTGGCAAATAGCACTGAGCCGGAGGTTCCGAGCATTGAGGCTATGGCTTGTTCTAAGAAGCTACGGCGTGGCTAACCTTAGAAACTTTATTCGTTGCCATGTCAAAATGGCCAAGACTTTTGAAGGGCTTGTGAGAATGGACAAGAGGTTTGAGATTGTGGTGCCTAGAGACTTCTCATTGGTCTGCTTTAGAATTTCACCATCGGCATTGATCAGTAGGGATGAGGATGGTGAGATCGGTATGATAAACGAGGTCAACTGCAAGCTGCTAGATGCCATCAACGCATCAGGTAAAGCATACATGACCCATGCTGTGGTTGGTGGTATCTTTCTGCTGCGGTGCGCCATTGGTGCTACTCTGACAGAAGAAAAGCACATAGTCCAGGCTTGGAAGGTGGTGCAAAACCATGCACAAGCCATCCTCAATATGCATTGA

Protein Analysis

510

Amino Acids

56.48

Weight (kDa)

6.14

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 58 - 431 6.9e-113 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 685
AccB1I GGYRCC 1 cut(s) 1258
AccB7I CCANNNNNTGG 1 cut(s) 1411
AccI GTMKAC 1 cut(s) 111
AciI CCGC 3 cut(s) 486, 792, 1435
AclWI GGATC 2 cut(s) 261, 803
AcoI YGGCCR 2 cut(s) 97, 1206
AcsI RAATTY 5 cut(s) 292, 574, 639, 695, 1290
AcuI CTGAAG 3 cut(s) 99, 431, 608
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 2 cut(s) 963, 1411
AgsI TTSAA 3 cut(s) 315, 379, 1220
AjnI CCWGG 3 cut(s) 172, 265, 1479
AluBI AGCT 7 cut(s) 200, 409, 451, 536, 632, 1158, 1363
AluI AGCT 7 cut(s) 200, 409, 451, 536, 632, 1158, 1363
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 2 cut(s) 537, 1260
AlwI GGATC 2 cut(s) 261, 803
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 5 cut(s) 97, 268, 513, 669, 1206
ApeKI GCWGC 7 cut(s) 398, 533, 536, 987, 990, 1363, 1432
ApoI RAATTY 5 cut(s) 292, 574, 639, 695, 1290
ArsI GACNNNNNNTTYG 4 cut(s) 405, 437, 1229, 1261
AspLEI GCGC 2 cut(s) 935, 1442
AspS9I GGNCC 1 cut(s) 669
AsuC2I CCSGG 1 cut(s) 668
AsuHPI GGTGA 7 cut(s) 52, 343, 353, 384, 699, 1287, 1340
AsuNHI GCTAGC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 1208
BanI GGYRCC 1 cut(s) 1258
BanII GRGCYC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 7 cut(s) 410, 523, 545, 974, 977, 1350, 1419
BccI CCATC 9 cut(s) 65, 242, 680, 860, 914, 1306, 1319, 1382, 1523
BceAI ACGGC 3 cut(s) 112, 862, 1177
BciT130I CCWGG 3 cut(s) 174, 267, 1481
BclI TGATCA 1 cut(s) 1308
BcnI CCSGG 1 cut(s) 668
BcoDI GTCTC 2 cut(s) 537, 1260
BfaI CTAG 6 cut(s) 71, 321, 743, 755, 1263, 1367
BfmI CTRYAG 1 cut(s) 327
BisI GCNGC 7 cut(s) 399, 534, 537, 988, 991, 1364, 1433
BlpI GCTNAGC 1 cut(s) 554
BlsI GCNGC 7 cut(s) 400, 535, 538, 989, 992, 1365, 1434
BmcAI AGTACT 1 cut(s) 505
Bme1390I CCNGG 4 cut(s) 174, 267, 668, 1481
BmgT120I GGNCC 1 cut(s) 669
BmiI GGNNCC 2 cut(s) 1124, 1260
BmrFI CCNGG 4 cut(s) 174, 267, 668, 1481
BmrI ACTGGG 2 cut(s) 146, 391
BmsI GCATC 3 cut(s) 705, 1360, 1391
BmtI GCTAGC 1 cut(s) 324
BmuI ACTGGG 2 cut(s) 146, 391
Bpu1102I GCTNAGC 1 cut(s) 554
BpuMI CCSGG 1 cut(s) 668
Bsa29I ATCGAT 1 cut(s) 918
BsaI GGTCTC 1 cut(s) 537
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 2 cut(s) 963, 1411
Bse1I ACTGG 6 cut(s) 152, 361, 386, 979, 1065, 1103
BseBI CCWGG 3 cut(s) 174, 267, 1481
BseCI ATCGAT 1 cut(s) 918
BseGI GGATG 5 cut(s) 234, 871, 1324, 1330, 1515
BseLI CCNNNNNNNGG 2 cut(s) 963, 1411
BseMII CTCAG 2 cut(s) 702, 1103
BseNI ACTGG 6 cut(s) 152, 361, 386, 979, 1065, 1103
BseXI GCAGC 7 cut(s) 410, 523, 545, 974, 977, 1350, 1419
BshFI GGCC 5 cut(s) 99, 270, 515, 671, 1208
BshNI GGYRCC 1 cut(s) 1258
BshVI ATCGAT 1 cut(s) 918
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 2 cut(s) 667, 1117
BslI CCNNNNNNNGG 2 cut(s) 963, 1411
BsmAI GTCTC 2 cut(s) 537, 1260
BsnI GGCC 5 cut(s) 99, 270, 515, 671, 1208
Bso31I GGTCTC 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 4 cut(s) 253, 808, 1308, 1332
Bsp1720I GCTNAGC 1 cut(s) 554
BspACI CCGC 3 cut(s) 486, 792, 1435
BspANI GGCC 5 cut(s) 99, 270, 515, 671, 1208
BspCNI CTCAG 2 cut(s) 703, 1104
BspDI ATCGAT 1 cut(s) 918
BspLI GGNNCC 2 cut(s) 1124, 1260
BspMAI CTGCAG 1 cut(s) 331
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 261, 803
BspT107I GGYRCC 1 cut(s) 1258
BspTNI GGTCTC 1 cut(s) 537
BsrI ACTGG 6 cut(s) 152, 361, 386, 979, 1065, 1103
BssMI GATC 4 cut(s) 253, 808, 1308, 1332
Bst2UI CCWGG 3 cut(s) 174, 267, 1481
Bst6I CTCTTC 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 3 cut(s) 322, 889, 1361
BstDEI CTNAG 7 cut(s) 158, 554, 711, 719, 1112, 1151, 1175
BstF5I GGATG 5 cut(s) 234, 871, 1324, 1330, 1515
BstHHI GCGC 2 cut(s) 935, 1442
BstKTI GATC 4 cut(s) 256, 811, 1311, 1335
BstMAI GTCTC 2 cut(s) 537, 1260
BstMBI GATC 4 cut(s) 253, 808, 1308, 1332
BstMWI GCNNNNNNNGC 7 cut(s) 326, 483, 800, 884, 930, 1379, 1448
BstNI CCWGG 3 cut(s) 174, 267, 1481
BstNSI RCATGY 2 cut(s) 445, 891
BstSCI CCNGG 4 cut(s) 172, 265, 666, 1479
BstSFI CTRYAG 1 cut(s) 327
BstV1I GCAGC 7 cut(s) 410, 523, 545, 974, 977, 1350, 1419
BstXI CCANNNNNNTGG 2 cut(s) 473, 644
Bsu15I ATCGAT 1 cut(s) 918
BsuRI GGCC 5 cut(s) 99, 270, 515, 671, 1208
BsuTUI ATCGAT 1 cut(s) 918
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 5 cut(s) 234, 871, 1324, 1330, 1515
BtsI GCAGTG 2 cut(s) 324, 798
BtsIMutI CAGTG 6 cut(s) 324, 354, 798, 972, 1072, 1109
Cac8I GCNNGC 3 cut(s) 322, 889, 1361
CaiI CAGNNNCTG 1 cut(s) 203
CfoI GCGC 2 cut(s) 935, 1442
Cfr13I GGNCC 1 cut(s) 669
ClaI ATCGAT 1 cut(s) 918
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 1 cut(s) 504
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 7 cut(s) 158, 554, 711, 719, 1112, 1151, 1175
DpnI GATC 4 cut(s) 255, 810, 1310, 1334
DpnII GATC 4 cut(s) 253, 808, 1308, 1332
DrdI GACNNNNNNGTC 1 cut(s) 685
DseDI GACNNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 2 cut(s) 97, 1206
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 411
Eco31I GGTCTC 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 409
Eco57I CTGAAG 3 cut(s) 99, 431, 608
EcoICRI GAGCTC 1 cut(s) 409
EcoRII CCWGG 3 cut(s) 172, 265, 1479
EcoT22I ATGCAT 2 cut(s) 893, 1530
EcoT38I GRGCYC 1 cut(s) 411
FauNDI CATATG 2 cut(s) 211, 874
FbaI TGATCA 1 cut(s) 1308
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 7 cut(s) 399, 534, 537, 988, 991, 1364, 1433
FokI GGATG 5 cut(s) 221, 878, 1331, 1337, 1502
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 7 cut(s) 399, 534, 537, 988, 991, 1364, 1433
FspBI CTAG 6 cut(s) 71, 321, 743, 755, 1263, 1367
GlaI GCGC 2 cut(s) 934, 1441
GluI GCNGC 7 cut(s) 399, 534, 537, 988, 991, 1364, 1433
HaeIII GGCC 5 cut(s) 99, 270, 515, 671, 1208
HapII CCGG 2 cut(s) 667, 1117
HhaI GCGC 2 cut(s) 935, 1442
Hin6I GCGC 2 cut(s) 933, 1440
HinP1I GCGC 2 cut(s) 933, 1440
HincII GTYRAC 3 cut(s) 690, 1035, 1354
HindII GTYRAC 3 cut(s) 690, 1035, 1354
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 5 cut(s) 32, 648, 1006, 1039, 1070
HpaII CCGG 2 cut(s) 667, 1117
HphI GGTGA 7 cut(s) 52, 343, 353, 384, 699, 1287, 1340
Hpy166II GTNNAC 5 cut(s) 112, 690, 1035, 1087, 1354
Hpy188I TCNGA 6 cut(s) 604, 627, 739, 1011, 1127, 1458
Hpy188III TCNNGA 4 cut(s) 251, 676, 899, 1043
Hpy8I GTNNAC 5 cut(s) 112, 690, 1035, 1087, 1354
HpyAV CCTTC 3 cut(s) 743, 1214, 1483
HpyF10VI GCNNNNNNNGC 7 cut(s) 326, 483, 800, 884, 930, 1379, 1448
HpyF3I CTNAG 7 cut(s) 158, 554, 711, 719, 1112, 1151, 1175
HspAI GCGC 2 cut(s) 933, 1440
Ksp22I TGATCA 1 cut(s) 1308
Kzo9I GATC 4 cut(s) 253, 808, 1308, 1332
Lsp1109I GCAGC 7 cut(s) 410, 523, 545, 974, 977, 1350, 1419
LweI GCATC 3 cut(s) 705, 1360, 1391
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 6 cut(s) 71, 321, 743, 755, 1263, 1367
MaeIII GTNAC 2 cut(s) 492, 1067
MalI GATC 4 cut(s) 255, 810, 1310, 1334
MboI GATC 4 cut(s) 253, 808, 1308, 1332
MboII GAAGA 4 cut(s) 92, 303, 758, 1475
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 1 cut(s) 1208
MluCI AATT 7 cut(s) 83, 292, 561, 574, 639, 695, 1290
MluNI TGGCCA 1 cut(s) 1208
MlyI GAGTC 1 cut(s) 1064
Mox20I TGGCCA 1 cut(s) 1208
Mph1103I ATGCAT 2 cut(s) 893, 1530
MscI TGGCCA 1 cut(s) 1208
MseI TTAA 4 cut(s) 240, 363, 951, 1002
MslI CAYNNNNRTG 3 cut(s) 422, 864, 977
Msp20I TGGCCA 1 cut(s) 1208
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 2 cut(s) 667, 1117
MspR9I CCNGG 4 cut(s) 174, 267, 668, 1481
MvaI CCWGG 3 cut(s) 174, 267, 1481
MwoI GCNNNNNNNGC 7 cut(s) 326, 483, 800, 884, 930, 1379, 1448
NciI CCSGG 1 cut(s) 668
NdeI CATATG 2 cut(s) 211, 874
NdeII GATC 4 cut(s) 253, 808, 1308, 1332
NheI GCTAGC 1 cut(s) 320
NlaIV GGNNCC 2 cut(s) 1124, 1260
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 1067
NsiI ATGCAT 2 cut(s) 893, 1530
NspI RCATGY 2 cut(s) 445, 891
PaeI GCATGC 1 cut(s) 891
PfeI GAWTC 4 cut(s) 32, 648, 1006, 1039
PflMI CCANNNNNTGG 1 cut(s) 1411
PkrI GCNGC 7 cut(s) 400, 535, 538, 989, 992, 1365, 1434
PleI GAGTC 1 cut(s) 1064
PpsI GAGTC 1 cut(s) 1064
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 3 cut(s) 172, 265, 1479
PspGI CCWGG 3 cut(s) 172, 265, 1479
PspN4I GGNNCC 2 cut(s) 1124, 1260
PspPI GGNCC 1 cut(s) 669
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 203
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
RseI CAYNNNNRTG 3 cut(s) 422, 864, 977
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 4 cut(s) 240, 363, 951, 1002
SatI GCNGC 7 cut(s) 399, 534, 537, 988, 991, 1364, 1433
Sau3AI GATC 4 cut(s) 253, 808, 1308, 1332
Sau96I GGNCC 1 cut(s) 669
ScaI AGTACT 1 cut(s) 505
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 4 cut(s) 174, 267, 668, 1481
SduI GDGCHC 1 cut(s) 411
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 3 cut(s) 705, 1360, 1391
SfcI CTRYAG 1 cut(s) 327
SmiMI CAYNNNNRTG 3 cut(s) 422, 864, 977
SphI GCATGC 1 cut(s) 891
Sse9I AATT 7 cut(s) 83, 292, 561, 574, 639, 695, 1290
SsiI CCGC 3 cut(s) 486, 792, 1435
SspMI CTAG 6 cut(s) 71, 321, 743, 755, 1263, 1367
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 4 cut(s) 172, 265, 666, 1479
TaqI TCGA 3 cut(s) 411, 907, 918
TasI AATT 7 cut(s) 83, 292, 561, 574, 639, 695, 1290
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 4 cut(s) 32, 648, 1006, 1039
Tru1I TTAA 4 cut(s) 240, 363, 951, 1002
Tru9I TTAA 4 cut(s) 240, 363, 951, 1002
TscAI CASTG 6 cut(s) 331, 361, 805, 979, 1072, 1116
TseFI GTSAC 1 cut(s) 1067
TseI GCWGC 7 cut(s) 398, 533, 536, 987, 990, 1363, 1432
Tsp45I GTSAC 1 cut(s) 1067
TspDTI ATGAA 6 cut(s) 54, 106, 378, 848, 904, 1017
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 6 cut(s) 331, 361, 805, 979, 1072, 1116
Van91I CCANNNNNTGG 1 cut(s) 1411
XapI RAATTY 5 cut(s) 292, 574, 639, 695, 1290
XceI RCATGY 2 cut(s) 445, 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 1412
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 6 cut(s) 71, 321, 743, 755, 1263, 1367
ZrmI AGTACT 1 cut(s) 505
Zsp2I ATGCAT 2 cut(s) 893, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.