FvH4_3g31500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004534
Physical Location & Seq
Forward (+)
25749888 .. 25751209
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31500.t1

Sequence Viewer

Length: 318 bp
ATGTTGCCAGACCGATGGCGACGACGCCTAGATCTCGACGCCGCTAGATCGTCGACGTCGTATCGGCTCAGTCGTCTCTCATGCATCAACAACGCTGGGCCCAGACTAATCATCAGCTGTGGTCGGAGTCGGGAGCTCCTCATCTATCTCTTCTCTGCTTCAATTTCTTCGCCAGACTCCTCCTCATTATCTCCTCCCATTTTTAAAGTTCTTTGCCTTATAATTGTGGTAGTAGCTTTCGAAAATCATGTGAATTTGGATATTGAAGCCTCTCTTAACCAAGAGATCATTCTACTAACCAAGAAATATGTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.85

Weight (kDa)

9.5

Isoelectric Point (pI)

68.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
AatII GACGTC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 53
AciI CCGC 1 cut(s) 42
AcsI RAATTY 1 cut(s) 253
AcyI GRCGYC 3 cut(s) 25, 39, 56
AgsI TTSAA 2 cut(s) 162, 266
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 3 cut(s) 117, 136, 236
AluI AGCT 3 cut(s) 117, 136, 236
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 80
AoxI GGCC 1 cut(s) 98
ApaI GGGCCC 1 cut(s) 102
ApoI RAATTY 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 98, 99
AsuII TTCGAA 1 cut(s) 240
BaeGI GKGCMC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 102, 138
Bbv12I GWGCWC 1 cut(s) 138
BccI CCATC 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 29, 45
BglII AGATCT 1 cut(s) 31
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 98, 99
BmiI GGNNCC 1 cut(s) 100
BmsI GCATC 1 cut(s) 93
Bpu14I TTCGAA 1 cut(s) 240
BsaHI GRCGYC 3 cut(s) 25, 39, 56
BseMII CTCAG 1 cut(s) 82
BseRI GAGGAG 4 cut(s) 128, 169, 172, 183
BseSI GKGCMC 1 cut(s) 102
BseYI CCCAGC 1 cut(s) 95
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 80
BsmBI CGTCTC 1 cut(s) 80
BsnI GGCC 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 240
Bsp120I GGGCCC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 102, 138
Bsp143I GATC 3 cut(s) 31, 47, 285
BspACI CCGC 1 cut(s) 42
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 100
BspT104I TTCGAA 1 cut(s) 240
BssMI GATC 3 cut(s) 31, 47, 285
BssNI GRCGYC 3 cut(s) 25, 39, 56
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 3 cut(s) 25, 39, 56
BstBI TTCGAA 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 68
BstKTI GATC 3 cut(s) 34, 50, 288
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 3 cut(s) 31, 47, 285
BstSLI GKGCMC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 15
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 1 cut(s) 100
Cfr13I GGNCC 2 cut(s) 98, 99
CseI GACGC 2 cut(s) 33, 47
CviAII CATG 2 cut(s) 81, 248
CviJI RGCY 6 cut(s) 67, 100, 117, 136, 236, 269
CviKI_1 RGCY 6 cut(s) 67, 100, 117, 136, 236, 269
DdeI CTNAG 1 cut(s) 68
DpnI GATC 3 cut(s) 33, 49, 287
DpnII GATC 3 cut(s) 31, 47, 285
DraI TTTAAA 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 136
Eco24I GRGCYC 2 cut(s) 102, 138
Eco53kI GAGCTC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 2 cut(s) 102, 138
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 2 cut(s) 84, 251
FaiI YATR 4 cut(s) 82, 221, 249, 309
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FatI CATG 2 cut(s) 80, 247
FblI GTMKAC 1 cut(s) 53
Fnu4HI GCNGC 1 cut(s) 42
FriOI GRGCYC 2 cut(s) 102, 138
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 2 cut(s) 29, 45
GluI GCNGC 1 cut(s) 42
GsaI CCCAGC 1 cut(s) 99
HaeIII GGCC 1 cut(s) 100
HgaI GACGC 2 cut(s) 33, 47
Hin1I GRCGYC 3 cut(s) 25, 39, 56
Hin1II CATG 2 cut(s) 84, 251
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 2 cut(s) 127, 176
Hpy166II GTNNAC 1 cut(s) 54
Hpy188I TCNGA 1 cut(s) 126
Hpy188III TCNNGA 2 cut(s) 35, 131
Hpy8I GTNNAC 1 cut(s) 54
Hpy99I CGWCG 6 cut(s) 24, 27, 41, 55, 58, 61
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 1 cut(s) 84
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 3 cut(s) 25, 39, 56
Hsp92II CATG 2 cut(s) 84, 251
Kzo9I GATC 3 cut(s) 31, 47, 285
LmnI GCTCC 2 cut(s) 133, 141
LpnPI CCDG 4 cut(s) 21, 81, 115, 186
LweI GCATC 1 cut(s) 93
MaeI CTAG 2 cut(s) 29, 45
MaeII ACGT 1 cut(s) 56
MalI GATC 3 cut(s) 33, 49, 287
MboI GATC 3 cut(s) 31, 47, 285
MboII GAAGA 2 cut(s) 142, 159
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 102, 138
MluCI AATT 3 cut(s) 162, 222, 253
MlyI GAGTC 2 cut(s) 136, 170
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 5 cut(s) 149, 190, 193, 204, 280
Mph1103I ATGCAT 1 cut(s) 86
MseI TTAA 2 cut(s) 204, 276
MspA1I CMGCKG 1 cut(s) 117
NdeII GATC 3 cut(s) 31, 47, 285
NlaIII CATG 2 cut(s) 84, 251
NlaIV GGNNCC 1 cut(s) 100
NsiI ATGCAT 1 cut(s) 86
NspV TTCGAA 1 cut(s) 240
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 70
PkrI GCNGC 1 cut(s) 43
PleI GAGTC 2 cut(s) 135, 170
PpsI GAGTC 2 cut(s) 135, 170
PsiI TTATAA 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 138
PspFI CCCAGC 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 100
PspOMI GGGCCC 1 cut(s) 98
PspPI GGNCC 2 cut(s) 98, 99
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 138
SalI GTCGAC 1 cut(s) 52
SaqAI TTAA 2 cut(s) 204, 276
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 3 cut(s) 31, 47, 285
Sau96I GGNCC 2 cut(s) 98, 99
SchI GAGTC 2 cut(s) 136, 170
SduI GDGCHC 2 cut(s) 102, 138
SetI ASST 4 cut(s) 59, 119, 138, 238
SfaNI GCATC 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 240
SgrDI CGTCGACG 1 cut(s) 52
Sse9I AATT 3 cut(s) 162, 222, 253
SsiI CCGC 1 cut(s) 42
SspMI CTAG 2 cut(s) 29, 45
SstI GAGCTC 1 cut(s) 138
TaiI ACGT 1 cut(s) 59
TaqI TCGA 3 cut(s) 36, 53, 240
TaqII GACCGA 1 cut(s) 27
TasI AATT 3 cut(s) 162, 222, 253
TauI GCSGC 1 cut(s) 44
Tru1I TTAA 2 cut(s) 204, 276
Tru9I TTAA 2 cut(s) 204, 276
XapI RAATTY 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 53
XspI CTAG 2 cut(s) 29, 45
ZraI GACGTC 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.