Rroxscaffold_2G00148900

caffeoyl-CoA O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004534
Physical Location & Seq
Forward (+)
86523087 .. 86525716
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148900.1

Sequence Viewer

Length: 744 bp
ATGGATGCAATGCATGTACCAAATTCATCACGGACCGCCACACCAATACCTCCATCTGATCCGAACTCGGACCCAAAACTGCCATCAACATTTATCTTGAGACGCCCACTGGGGGGAGCTGCCATCTGGACTTGGGTCGTGCACACTTGGTCATACTTACTGGGTGTTGCTTCTGATAATCAGACAAAAAGTATGAGACCATCTAACTGCATTGCATGCATTGAAGTACGTACCCTTCCACAAAGTGTTGTTGCGCTCGATCCAAATGACCCACAGATGCATGAAGAAAAGATCACGCTGACTACGTCGATGGATTGGAACAATCTTGATGAGGAGATGAATAGAAGGATGCGAGAGAGCAGAAGAAAAAGAATATTAAATTGGACTTCTCATGATGTGGCACAGTATATACTGAAGACTGGTGTCTACCCAAGAGAACCAAATCCTCTCAAGGAACTGAGGATTGCTACTGCAAATCACCCTATGTCAGTTAATGGCTATGCTCTTAAACTAGTGAATCCCAAAAATGCAATTGAAATCGGAGTTTTTACAGGATACTCTCTTCTCCTCACTGCTCTCACGATTCCTGATTATGGCAAGATTACAGCCATAGACATAAACCGGAAAGCATATGAAATAGGCTTACCAATCGTCAAAAAAGCGGGTGTAGAGCACAAAATTGACTTCATTGAGTCCCCAGCTCTGCCTATTCTTGACAAACTCTTGGAAAATGTAGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00360 ko00940 ko00941 ko00945 ko01100 ko01110 map00360 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Methyltransf_3

Protein Analysis

247

Amino Acids

27.73

Weight (kDa)

7.78

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 167 - 244 5.1e-28 O-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 426, 735
AciI CCGC 2 cut(s) 36, 662
AclWI GGATC 2 cut(s) 53, 254
AcsI RAATTY 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 103
AdeI CACNNNGTG 1 cut(s) 245
AfaI GTAC 3 cut(s) 18, 228, 232
AfiI CCNNNNNNNGG 3 cut(s) 112, 113, 593
AgsI TTSAA 2 cut(s) 224, 536
AhlI ACTAGT 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 364, 396
AluBI AGCT 2 cut(s) 119, 701
AluI AGCT 2 cut(s) 119, 701
Alw21I GWGCWC 2 cut(s) 144, 675
Alw26I GTCTC 2 cut(s) 94, 190
Alw44I GTGCAC 1 cut(s) 140
AlwI GGATC 2 cut(s) 53, 254
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 22
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 33, 70
AsuHPI GGTGA 1 cut(s) 470
AvaII GGWCC 2 cut(s) 33, 70
BaeGI GKGCMC 1 cut(s) 144
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 2 cut(s) 144, 675
BbvI GCAGC 1 cut(s) 106
BccI CCATC 5 cut(s) 61, 91, 131, 208, 304
BciVI GTATCC 1 cut(s) 548
BcoDI GTCTC 2 cut(s) 94, 190
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 1 cut(s) 512
BfuI GTATCC 1 cut(s) 548
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme18I GGWCC 2 cut(s) 33, 70
BmgT120I GGNCC 2 cut(s) 33, 70
BmiI GGNNCC 1 cut(s) 72
BmrI ACTGGG 2 cut(s) 119, 170
BmsI GCATC 2 cut(s) 267, 339
BmuI ACTGGG 2 cut(s) 119, 170
BoxI GACNNNNGTC 2 cut(s) 134, 422
BpiI GAAGAC 1 cut(s) 422
BpuEI CTTGAG 2 cut(s) 118, 434
BsaAI YACGTR 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 103
BsaI GGTCTC 1 cut(s) 190
BsaWI WCCGGW 1 cut(s) 621
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 113, 593
Bse1I ACTGG 3 cut(s) 114, 165, 424
Bse3DI GCAATG 2 cut(s) 15, 210
BseGI GGATG 2 cut(s) 10, 354
BseLI CCNNNNNNNGG 3 cut(s) 112, 113, 593
BseMI GCAATG 2 cut(s) 15, 210
BseMII CTCAG 1 cut(s) 449
BseNI ACTGG 3 cut(s) 114, 165, 424
BseRI GAGGAG 2 cut(s) 347, 557
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 1 cut(s) 106
BseYI CCCAGC 1 cut(s) 697
BsiHKAI GWGCWC 2 cut(s) 144, 675
BsiSI CCGG 1 cut(s) 622
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 3 cut(s) 112, 113, 593
BsmAI GTCTC 2 cut(s) 94, 190
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 679
Bso31I GGTCTC 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 144, 675
Bsp143I GATC 3 cut(s) 58, 259, 291
BspACI CCGC 2 cut(s) 36, 662
BspCNI CTCAG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 2 cut(s) 53, 254
BspTNI GGTCTC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 15, 210
BsrI ACTGG 3 cut(s) 114, 165, 424
BssMI GATC 3 cut(s) 58, 259, 291
BssNI GRCGYC 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 405
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 103
BstAPI GCANNNNNTGC 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 458
BstF5I GGATG 2 cut(s) 10, 354
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 61, 262, 294
BstMAI GTCTC 2 cut(s) 94, 190
BstMBI GATC 3 cut(s) 58, 259, 291
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNSI RCATGY 2 cut(s) 17, 219
BstPAI GACNNNNGTC 2 cut(s) 134, 422
BstSLI GKGCMC 1 cut(s) 144
BstSNI TACGTA 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 106
BstV2I GAAGAC 1 cut(s) 422
BsuI GTATCC 1 cut(s) 548
BtsCI GGATG 2 cut(s) 10, 354
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 2 cut(s) 107, 570
Cac8I GCNNGC 1 cut(s) 217
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 2 cut(s) 33, 70
CpoI CGGWCCG 1 cut(s) 33
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 17, 227, 231
CspI CGGWCCG 1 cut(s) 33
CviAII CATG 4 cut(s) 14, 216, 281, 392
CviJI RGCY 5 cut(s) 119, 498, 608, 642, 701
CviKI_1 RGCY 5 cut(s) 119, 498, 608, 642, 701
CviQI GTAC 3 cut(s) 17, 227, 231
DdeI CTNAG 1 cut(s) 458
DpnI GATC 3 cut(s) 60, 261, 293
DpnII GATC 3 cut(s) 58, 259, 291
DraIII CACNNNGTG 1 cut(s) 245
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco105I TACGTA 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 190
Eco47I GGWCC 2 cut(s) 33, 70
Eco57I CTGAAG 1 cut(s) 434
EcoT22I ATGCAT 3 cut(s) 15, 221, 282
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 4 cut(s) 17, 219, 284, 395
FaqI GGGAC 1 cut(s) 679
FatI CATG 4 cut(s) 13, 215, 280, 391
FauI CCCGC 1 cut(s) 655
FauNDI CATATG 1 cut(s) 631
FblI GTMKAC 2 cut(s) 426, 735
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 2 cut(s) 17, 361
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 512
GlaI GCGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 120
GsaI CCCAGC 1 cut(s) 701
HapII CCGG 1 cut(s) 622
HgaI GACGC 1 cut(s) 111
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 4 cut(s) 17, 219, 284, 395
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HinfI GANTC 3 cut(s) 517, 583, 692
HpaII CCGG 1 cut(s) 622
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 3 cut(s) 142, 427, 736
Hpy188I TCNGA 6 cut(s) 58, 63, 70, 175, 183, 542
Hpy188III TCNNGA 7 cut(s) 97, 127, 326, 392, 580, 587, 713
Hpy8I GTNNAC 3 cut(s) 142, 427, 736
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 2 cut(s) 245, 339
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4IV ACGT 2 cut(s) 229, 305
HpyCH4V TGCA 9 cut(s) 8, 13, 142, 210, 215, 219, 280, 473, 530
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 2 cut(s) 229, 305
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 4 cut(s) 17, 219, 284, 395
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 3 cut(s) 58, 259, 291
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 8 cut(s) 95, 112, 146, 405, 537, 600, 635, 711
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 2 cut(s) 267, 339
MaeI CTAG 1 cut(s) 512
MaeII ACGT 2 cut(s) 229, 305
MalI GATC 3 cut(s) 60, 261, 293
MboI GATC 3 cut(s) 58, 259, 291
MboII GAAGA 4 cut(s) 296, 375, 427, 554
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 144, 675
MluCI AATT 4 cut(s) 22, 379, 531, 678
MlyI GAGTC 1 cut(s) 701
MnlI CCTC 5 cut(s) 60, 325, 453, 456, 578
Mph1103I ATGCAT 3 cut(s) 15, 221, 282
MseI TTAA 3 cut(s) 377, 492, 507
MspI CCGG 1 cut(s) 622
MunI CAATTG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 216
NdeI CATATG 1 cut(s) 631
NdeII GATC 3 cut(s) 58, 259, 291
NlaIII CATG 4 cut(s) 17, 219, 284, 395
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 3 cut(s) 15, 221, 282
NspI RCATGY 2 cut(s) 17, 219
PaeI GCATGC 1 cut(s) 219
PagI TCATGA 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PfeI GAWTC 2 cut(s) 517, 583
PflFI GACNNNGTC 1 cut(s) 304
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Ppu21I YACGTR 1 cut(s) 230
PshAI GACNNNNGTC 2 cut(s) 134, 422
PspFI CCCAGC 1 cut(s) 697
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 33, 70
PsyI GACNNNGTC 1 cut(s) 304
RsaI GTAC 3 cut(s) 18, 228, 232
RsaNI GTAC 3 cut(s) 17, 227, 231
Rsr2I CGGWCCG 1 cut(s) 33
RsrII CGGWCCG 1 cut(s) 33
SaqAI TTAA 3 cut(s) 377, 492, 507
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 3 cut(s) 58, 259, 291
Sau96I GGNCC 2 cut(s) 33, 70
SchI GAGTC 1 cut(s) 701
SduI GDGCHC 2 cut(s) 144, 675
SetI ASST 5 cut(s) 52, 121, 232, 308, 703
SfaNI GCATC 2 cut(s) 267, 339
SinI GGWCC 2 cut(s) 33, 70
SmlI CTYRAG 2 cut(s) 97, 449
SmoI CTYRAG 2 cut(s) 97, 449
SnaBI TACGTA 1 cut(s) 230
SpeI ACTAGT 1 cut(s) 511
SphI GCATGC 1 cut(s) 219
Sse9I AATT 4 cut(s) 22, 379, 531, 678
SsiI CCGC 2 cut(s) 36, 662
SspI AATATT 1 cut(s) 375
SspMI CTAG 1 cut(s) 512
TaaI ACNGT 1 cut(s) 405
TaiI ACGT 2 cut(s) 232, 308
TaqI TCGA 2 cut(s) 258, 308
TasI AATT 4 cut(s) 22, 379, 531, 678
TfiI GAWTC 2 cut(s) 517, 583
Tru1I TTAA 3 cut(s) 377, 492, 507
Tru9I TTAA 3 cut(s) 377, 492, 507
TscAI CASTG 2 cut(s) 114, 577
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 5 cut(s) 15, 297, 353, 648, 676
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 2 cut(s) 114, 577
Tth111I GACNNNGTC 1 cut(s) 304
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 33, 70
XapI RAATTY 1 cut(s) 22
XceI RCATGY 2 cut(s) 17, 219
XmiI GTMKAC 2 cut(s) 426, 735
XspI CTAG 1 cut(s) 512
Zsp2I ATGCAT 3 cut(s) 15, 221, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.