Rroxscaffold_1G00031690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004534
Physical Location & Seq
Reverse (-)
44953658 .. 44954833
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031690.1

Sequence Viewer

Length: 714 bp
ATGGATGTTGTTGATGCTGCTGCTGCTGCTACTAGGCTACTAGAGTCTTCAGATAGATTTGTGTTGTCGGAATCAAAGAAACTGGAAATCTCTACTACGGTTTGGGGTGGCGGCGTCTCCTTTGAGATCGTCCTTCCTTGTTTCTACTCCACCTCAATGGAGTCATTCACGGGTTTTGGTCGGTCTCTTGCTATTCTTCCCGCCTCTCGCAAAACATCTCTCGCTTACGGAGACTCGCGACGGAGGCTCGAGTTCGGCCTTGAAGAAGTGCAAGGCCAATCATGGCCTTCGAGAGGTCGTCCACGGTGGTGTTCGCGCGATGGTGACGATGGGCGTGTATTCTCTTCGAGCTCGACCGTTGGGGGAATATGGGATCATGGTGATTTTGGTCTTTGGTCTTGTTTATGCGGGTTGGTTCATACTCTCTCTTCGAATTCTCCACGTCAATGGTGGGTGGTGATCGGGGAGCGAGCCGGCGGTGGTGGTGGGCTAGCAATCGGGGATCAAGCGGCGGCTTTGCGGAAGGACCGGATCCGGATGCGGGATCCGGTGGTTTTGGCAAACGGGTTCGGATGCGGGATCCGGTGGAGATTGGGTTTGGTCTATGACCCACTATCTTTGGTGGGTTGTATATCGATTGCTAGATTGGTTTGGGACTTTTGTGCGTTATCGATATTGGATTCGAGACCCATGTCTATGGTATTCTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

25.84

Weight (kDa)

8.15

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 238, 316, 318
AccIII TCCGGA 1 cut(s) 534
AciI CCGC 9 cut(s) 111, 201, 408, 477, 509, 512, 520, 541, 576
AclWI GGATC 8 cut(s) 381, 510, 526, 539, 539, 552, 574, 587
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 293, 541
AgsI TTSAA 1 cut(s) 263
AjiI CACGTC 1 cut(s) 443
AleI CACNNNNGTG 1 cut(s) 307
AluBI AGCT 1 cut(s) 351
AluI AGCT 1 cut(s) 351
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 4 cut(s) 121, 189, 225, 679
AlwI GGATC 8 cut(s) 381, 510, 526, 539, 539, 552, 574, 587
Ama87I CYCGRG 1 cut(s) 248
Aor13HI TCCGGA 1 cut(s) 534
AoxI GGCC 3 cut(s) 256, 274, 284
ApeKI GCWGC 4 cut(s) 17, 20, 23, 26
ApoI RAATTY 1 cut(s) 433
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 3 cut(s) 335, 392, 469
AsuII TTCGAA 1 cut(s) 431
AsuNHI GCTAGC 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 526
BamHI GGATCC 3 cut(s) 531, 544, 579
BanII GRGCYC 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 4 cut(s) 4, 7, 10, 13
BccI CCATC 2 cut(s) 314, 323
BcoDI GTCTC 4 cut(s) 121, 189, 225, 679
BfaI CTAG 4 cut(s) 33, 41, 491, 642
BisI GCNGC 7 cut(s) 18, 21, 24, 27, 112, 510, 513
BlsI GCNGC 7 cut(s) 19, 22, 25, 28, 113, 511, 514
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 3 cut(s) 533, 546, 581
BmsI GCATC 3 cut(s) 4, 528, 563
BmtI GCTAGC 1 cut(s) 494
BoxI GACNNNNGTC 1 cut(s) 691
BpiI GAAGAC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 431
Bsa29I ATCGAT 2 cut(s) 635, 671
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 2 cut(s) 189, 679
BsaJI CCNNGG 1 cut(s) 302
BsaWI WCCGGW 4 cut(s) 528, 534, 547, 582
BsaXI ACNNNNNCTCC 2 cut(s) 580, 610
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 541
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 534
BseCI ATCGAT 2 cut(s) 635, 671
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 3 cut(s) 10, 543, 578
BseLI CCNNNNNNNGG 2 cut(s) 293, 541
BseNI ACTGG 1 cut(s) 87
BseXI GCAGC 4 cut(s) 4, 7, 10, 13
Bsh1236I CGCG 3 cut(s) 238, 316, 318
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 3 cut(s) 258, 276, 286
BshVI ATCGAT 2 cut(s) 635, 671
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 353
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 5 cut(s) 474, 529, 535, 548, 583
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 2 cut(s) 293, 541
BsmAI GTCTC 4 cut(s) 121, 189, 225, 679
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 3 cut(s) 258, 276, 286
Bso31I GGTCTC 2 cut(s) 189, 679
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 353
Bsp13I TCCGGA 1 cut(s) 534
Bsp143I GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
Bsp68I TCGCGA 1 cut(s) 238
BspACI CCGC 9 cut(s) 111, 201, 408, 477, 509, 512, 520, 541, 576
BspANI GGCC 3 cut(s) 258, 276, 286
BspDI ATCGAT 2 cut(s) 635, 671
BspEI TCCGGA 1 cut(s) 534
BspFNI CGCG 3 cut(s) 238, 316, 318
BspLI GGNNCC 3 cut(s) 533, 546, 581
BspOI GCTAGC 1 cut(s) 494
BspPI GGATC 8 cut(s) 381, 510, 526, 539, 539, 552, 574, 587
BspT104I TTCGAA 1 cut(s) 431
BspTNI GGTCTC 2 cut(s) 189, 679
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
BssNI GRCGYC 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 100, 306, 358
Bst6I CTCTTC 2 cut(s) 349, 433
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 3 cut(s) 471, 475, 492
BstDSI CCRYGG 1 cut(s) 302
BstENI CCTNNNNNAGG 1 cut(s) 291
BstF5I GGATG 3 cut(s) 10, 543, 578
BstFNI CGCG 3 cut(s) 238, 316, 318
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 7 cut(s) 129, 376, 462, 505, 534, 547, 582
BstMAI GTCTC 4 cut(s) 121, 189, 225, 679
BstMBI GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 23, 26, 244
BstPAI GACNNNNGTC 1 cut(s) 691
BstUI CGCG 3 cut(s) 238, 316, 318
BstV1I GCAGC 4 cut(s) 4, 7, 10, 13
BstV2I GAAGAC 1 cut(s) 39
BstX2I RGATCY 3 cut(s) 531, 544, 579
BstXI CCANNNNNNTGG 3 cut(s) 157, 447, 697
BstYI RGATCY 3 cut(s) 531, 544, 579
Bsu15I ATCGAT 2 cut(s) 635, 671
BsuRI GGCC 3 cut(s) 258, 276, 286
BsuTUI ATCGAT 2 cut(s) 635, 671
BtgI CCRYGG 1 cut(s) 302
BtgZI GCGATG 1 cut(s) 333
BtrI CACGTC 1 cut(s) 443
BtsCI GGATG 3 cut(s) 10, 543, 578
BtuMI TCGCGA 1 cut(s) 238
Cac8I GCNNGC 3 cut(s) 471, 475, 492
CfoI GCGC 1 cut(s) 318
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 526
ClaI ATCGAT 2 cut(s) 635, 671
CseI GACGC 1 cut(s) 103
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CviAII CATG 3 cut(s) 282, 377, 691
CviJI RGCY 9 cut(s) 37, 247, 258, 276, 286, 351, 473, 490, 515
CviKI_1 RGCY 9 cut(s) 37, 247, 258, 276, 286, 351, 473, 490, 515
DpnI GATC 7 cut(s) 128, 375, 461, 504, 533, 546, 581
DpnII GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
Eam1104I CTCTTC 2 cut(s) 349, 433
EarI CTCTTC 2 cut(s) 349, 433
Ecl136II GAGCTC 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 353
Eco31I GGTCTC 2 cut(s) 189, 679
Eco47I GGWCC 1 cut(s) 526
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 353
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 3 cut(s) 285, 380, 694
FaiI YATR 9 cut(s) 283, 370, 378, 406, 420, 606, 632, 692, 698
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 281, 376, 690
FauI CCCGC 4 cut(s) 208, 401, 534, 569
Fnu4HI GCNGC 7 cut(s) 18, 21, 24, 27, 112, 510, 513
FokI GGATG 3 cut(s) 17, 550, 585
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 7 cut(s) 18, 21, 24, 27, 112, 510, 513
FspBI CTAG 4 cut(s) 33, 41, 491, 642
GlaI GCGC 1 cut(s) 317
GluI GCNGC 7 cut(s) 18, 21, 24, 27, 112, 510, 513
HaeIII GGCC 3 cut(s) 258, 276, 286
HapII CCGG 5 cut(s) 474, 529, 535, 548, 583
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 3 cut(s) 285, 380, 694
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 5 cut(s) 44, 71, 161, 233, 680
HpaII CCGG 5 cut(s) 474, 529, 535, 548, 583
HphI GGTGA 3 cut(s) 335, 392, 469
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 3 cut(s) 52, 70, 572
Hpy188III TCNNGA 4 cut(s) 237, 291, 535, 684
Hpy8I GTNNAC 1 cut(s) 302
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 3 cut(s) 143, 297, 517
HpyCH4III ACNGT 3 cut(s) 100, 306, 358
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 1 cut(s) 271
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 26, 244
HpySE526I ACGT 1 cut(s) 442
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 3 cut(s) 285, 380, 694
HspAI GCGC 1 cut(s) 316
Kpn2I TCCGGA 1 cut(s) 534
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Kzo9I GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 6 cut(s) 68, 487, 542, 548, 561, 596
Lsp1109I GCAGC 4 cut(s) 4, 7, 10, 13
LweI GCATC 3 cut(s) 4, 528, 563
MaeI CTAG 4 cut(s) 33, 41, 491, 642
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 1 cut(s) 323
MalI GATC 7 cut(s) 128, 375, 461, 504, 533, 546, 581
MboI GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
MboII GAAGA 5 cut(s) 39, 188, 275, 336, 420
MflI RGATCY 3 cut(s) 531, 544, 579
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 433
MlyI GAGTC 3 cut(s) 53, 170, 227
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 4 cut(s) 163, 214, 237, 287
MroI TCCGGA 1 cut(s) 534
MroNI GCCGGC 1 cut(s) 473
MslI CAYNNNNRTG 4 cut(s) 155, 307, 445, 695
MspI CCGG 5 cut(s) 474, 529, 535, 548, 583
MvnI CGCG 3 cut(s) 238, 316, 318
MwoI GCNNNNNNNGC 3 cut(s) 23, 26, 244
NaeI GCCGGC 1 cut(s) 475
NdeII GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
NgoMIV GCCGGC 1 cut(s) 473
NheI GCTAGC 1 cut(s) 490
NlaIII CATG 3 cut(s) 285, 380, 694
NlaIV GGNNCC 3 cut(s) 533, 546, 581
NmuCI GTSAC 1 cut(s) 323
NruI TCGCGA 1 cut(s) 238
NspV TTCGAA 1 cut(s) 431
OliI CACNNNNGTG 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 248
PdiI GCCGGC 1 cut(s) 475
PfeI GAWTC 2 cut(s) 71, 680
PkrI GCNGC 7 cut(s) 19, 22, 25, 28, 113, 511, 514
PleI GAGTC 3 cut(s) 52, 169, 227
PpsI GAGTC 3 cut(s) 52, 169, 227
PshAI GACNNNNGTC 1 cut(s) 691
Psp124BI GAGCTC 1 cut(s) 353
PspN4I GGNNCC 3 cut(s) 533, 546, 581
PspPI GGNCC 1 cut(s) 526
PspXI VCTCGAGB 1 cut(s) 248
PsuI RGATCY 3 cut(s) 531, 544, 579
RruI TCGCGA 1 cut(s) 238
RseI CAYNNNNRTG 4 cut(s) 155, 307, 445, 695
SacI GAGCTC 1 cut(s) 353
SatI GCNGC 7 cut(s) 18, 21, 24, 27, 112, 510, 513
Sau3AI GATC 7 cut(s) 126, 373, 459, 502, 531, 544, 579
Sau96I GGNCC 1 cut(s) 526
SchI GAGTC 3 cut(s) 53, 170, 227
SduI GDGCHC 1 cut(s) 353
SetI ASST 4 cut(s) 155, 298, 353, 445
SfaNI GCATC 3 cut(s) 4, 528, 563
Sfr274I CTCGAG 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 1 cut(s) 526
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 4 cut(s) 155, 307, 445, 695
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 1 cut(s) 433
SsiI CCGC 9 cut(s) 111, 201, 408, 477, 509, 512, 520, 541, 576
SspMI CTAG 4 cut(s) 33, 41, 491, 642
SstI GAGCTC 1 cut(s) 353
TaaI ACNGT 3 cut(s) 100, 306, 358
TaiI ACGT 1 cut(s) 445
TaqI TCGA 8 cut(s) 249, 290, 347, 353, 431, 635, 671, 683
TaqII GACCGA 1 cut(s) 171
TasI AATT 1 cut(s) 433
TauI GCSGC 3 cut(s) 114, 512, 515
TfiI GAWTC 2 cut(s) 71, 680
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 4 cut(s) 17, 20, 23, 26
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 2 cut(s) 243, 256
VpaK11BI GGWCC 1 cut(s) 526
XagI CCTNNNNNAGG 1 cut(s) 291
XapI RAATTY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 447
XhoI CTCGAG 1 cut(s) 248
XspI CTAG 4 cut(s) 33, 41, 491, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.