FvH4_3g37440

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002651
Physical Location & Seq
Reverse (-)
32129571 .. 32132301
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37440.t1

Sequence Viewer

Length: 543 bp
ATGATGTTGGCTGTTGTTGAAAGTCATAGGCATTCTGTGAGTGACGTGTCGGTCTCGGATGAGTCCACGTTTGATTCTTGTGGGTCGGATTGTTGCTCTTTGGAGGGTGTGGATTTGGAGAGTGGGGTTTTGGAGCTGAAGCAGAAGCTGGTGGAGTTGAGTAAGGTTGAGAGGATTTGCAGGATATGCCACTTGGGTTATGAGGGTGGAGGGTTGGACTCTTCTGGGGTCCCAATTGACTTGGGGTGCGCTTGCAAAGGTGACTTGGGTGCTGCTCATAAGCAATGTGCTGAGACTTGGTTCAAGATCAAGGGTGATACTATCTGTGAGATCTGTGGCGTCGTTGCATTCAACATACATGGTGAGCAGACCAATGAGGGAAATGGAGCCACCGCTCCGACGTCCCCTTCACCGGCAGCAGCATCTGTGATCATGGTGGAAACTCAAACCATGTGGCATGGCCGCCGCATTATGAATTTCCTGCTTGCTTGCATGATCGTTGCTTTTGTAATCTCATGGCTGTTCCACTTCAAAGTACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

181

Amino Acids

19.34

Weight (kDa)

5.14

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 60 - 112 3.3e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
AatII GACGTC 1 cut(s) 404
AccBSI CCGCTC 1 cut(s) 395
AciI CCGC 3 cut(s) 393, 463, 466
AcoI YGGCCR 1 cut(s) 460
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 2 cut(s) 339, 401
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 4 cut(s) 20, 304, 352, 532
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 2 cut(s) 136, 148
AluI AGCT 2 cut(s) 136, 148
Alw26I GTCTC 2 cut(s) 58, 287
AlwNI CAGNNNCTG 2 cut(s) 148, 425
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 3 cut(s) 272, 416, 419
ApoI RAATTY 1 cut(s) 475
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 229
AsuHPI GGTGA 4 cut(s) 272, 326, 374, 402
AvaII GGWCC 1 cut(s) 229
BbvI GCAGC 3 cut(s) 259, 428, 431
BclI TGATCA 1 cut(s) 429
BcoDI GTCTC 2 cut(s) 58, 287
BglII AGATCT 1 cut(s) 330
BisI GCNGC 5 cut(s) 273, 417, 420, 463, 466
BlsI GCNGC 5 cut(s) 274, 418, 421, 464, 467
BmcAI AGTACT 1 cut(s) 537
Bme18I GGWCC 1 cut(s) 229
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 229
BmiI GGNNCC 3 cut(s) 230, 231, 388
BmsI GCATC 1 cut(s) 431
BsaHI GRCGYC 2 cut(s) 339, 401
BsaI GGTCTC 1 cut(s) 58
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 412
Bse3DI GCAATG 1 cut(s) 290
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 282
BseXI GCAGC 3 cut(s) 259, 428, 431
BshFI GGCC 1 cut(s) 462
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 2 cut(s) 215, 388
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 2 cut(s) 58, 287
BsmFI GGGAC 2 cut(s) 215, 388
BsmI GAATGC 2 cut(s) 31, 347
BsnI GGCC 1 cut(s) 462
Bso31I GGTCTC 1 cut(s) 58
Bsp143I GATC 4 cut(s) 306, 330, 429, 495
BspACI CCGC 3 cut(s) 393, 463, 466
BspANI GGCC 1 cut(s) 462
BspCNI CTCAG 1 cut(s) 283
BspLI GGNNCC 3 cut(s) 230, 231, 388
BspTNI GGTCTC 1 cut(s) 58
BsrBI CCGCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 412
BssMI GATC 4 cut(s) 306, 330, 429, 495
BssNI GRCGYC 2 cut(s) 339, 401
Bst6I CTCTTC 1 cut(s) 226
BstACI GRCGYC 2 cut(s) 339, 401
BstAPI GCANNNNNTGC 1 cut(s) 186
BstC8I GCNNGC 3 cut(s) 253, 486, 490
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 64
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 4 cut(s) 309, 333, 432, 498
BstMAI GTCTC 2 cut(s) 58, 287
BstMBI GATC 4 cut(s) 306, 330, 429, 495
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 259, 428, 431
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 1 cut(s) 462
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 253, 486, 490
CaiI CAGNNNCTG 2 cut(s) 148, 425
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 1 cut(s) 229
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 536
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviAII CATG 6 cut(s) 359, 433, 451, 458, 493, 516
CviJI RGCY 6 cut(s) 11, 136, 148, 389, 462, 520
CviKI_1 RGCY 6 cut(s) 11, 136, 148, 389, 462, 520
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 1 cut(s) 291
DpnI GATC 4 cut(s) 308, 332, 431, 497
DpnII GATC 4 cut(s) 306, 330, 429, 495
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
EaeI YGGCCR 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco31I GGTCTC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 229
FaeI CATG 6 cut(s) 362, 436, 454, 461, 496, 519
FaqI GGGAC 2 cut(s) 215, 388
FatI CATG 6 cut(s) 358, 432, 450, 457, 492, 515
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 5 cut(s) 273, 417, 420, 463, 466
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 5 cut(s) 273, 417, 420, 463, 466
GlaI GCGC 1 cut(s) 250
GluI GCNGC 5 cut(s) 273, 417, 420, 463, 466
HaeIII GGCC 1 cut(s) 462
HapII CCGG 1 cut(s) 413
HgaI GACGC 1 cut(s) 328
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 2 cut(s) 339, 401
Hin1II CATG 6 cut(s) 362, 436, 454, 461, 496, 519
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 3 cut(s) 62, 74, 218
HpaII CCGG 1 cut(s) 413
HphI GGTGA 4 cut(s) 272, 326, 374, 402
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 3 cut(s) 58, 88, 399
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 66
Hpy99I CGWCG 2 cut(s) 344, 403
HpyAV CCTTC 1 cut(s) 417
HpyCH4IV ACGT 3 cut(s) 45, 68, 401
HpyCH4V TGCA 4 cut(s) 180, 255, 347, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 3 cut(s) 45, 68, 401
Hsp92I GRCGYC 2 cut(s) 339, 401
Hsp92II CATG 6 cut(s) 362, 436, 454, 461, 496, 519
HspAI GCGC 1 cut(s) 249
KflI GGGWCCC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 4 cut(s) 306, 330, 429, 495
LmnI GCTCC 3 cut(s) 133, 386, 400
LpnPI CCDG 5 cut(s) 134, 166, 210, 426, 494
Lsp1109I GCAGC 3 cut(s) 259, 428, 431
LweI GCATC 1 cut(s) 431
MaeII ACGT 3 cut(s) 45, 68, 401
MaeIII GTNAC 2 cut(s) 41, 260
MalI GATC 4 cut(s) 308, 332, 431, 497
MbiI CCGCTC 1 cut(s) 395
MboI GATC 4 cut(s) 306, 330, 429, 495
MboII GAAGA 1 cut(s) 213
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 330
MluCI AATT 2 cut(s) 234, 475
MlyI GAGTC 2 cut(s) 71, 212
MmeI TCCRAC 3 cut(s) 66, 195, 422
MnlI CCTC 5 cut(s) 97, 165, 196, 203, 370
MspI CCGG 1 cut(s) 413
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 2 cut(s) 31, 347
MwoI GCNNNNNNNGC 1 cut(s) 186
NdeII GATC 4 cut(s) 306, 330, 429, 495
NlaIII CATG 6 cut(s) 362, 436, 454, 461, 496, 519
NlaIV GGNNCC 3 cut(s) 230, 231, 388
NmuCI GTSAC 2 cut(s) 41, 260
PctI GAATGC 2 cut(s) 31, 347
PfeI GAWTC 1 cut(s) 74
PkrI GCNGC 5 cut(s) 274, 418, 421, 464, 467
PleI GAGTC 2 cut(s) 70, 212
PpsI GAGTC 2 cut(s) 70, 212
PpuMI RGGWCCY 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 230, 231, 388
PspPI GGNCC 1 cut(s) 229
PspPPI RGGWCCY 1 cut(s) 229
PstNI CAGNNNCTG 2 cut(s) 148, 425
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SatI GCNGC 5 cut(s) 273, 417, 420, 463, 466
Sau3AI GATC 4 cut(s) 306, 330, 429, 495
Sau96I GGNCC 1 cut(s) 229
ScaI AGTACT 1 cut(s) 537
SchI GAGTC 2 cut(s) 71, 212
SetI ASST 7 cut(s) 48, 71, 138, 150, 168, 262, 404
SfaNI GCATC 1 cut(s) 431
SinI GGWCC 1 cut(s) 229
Sse9I AATT 2 cut(s) 234, 475
SsiI CCGC 3 cut(s) 393, 463, 466
TaiI ACGT 3 cut(s) 48, 71, 404
TaqII GACCGA 1 cut(s) 40
TasI AATT 2 cut(s) 234, 475
TatI WGTACW 1 cut(s) 535
TauI GCSGC 2 cut(s) 465, 468
TfiI GAWTC 1 cut(s) 74
TseFI GTSAC 2 cut(s) 41, 260
TseI GCWGC 3 cut(s) 272, 416, 419
Tsp45I GTSAC 2 cut(s) 41, 260
TspDTI ATGAA 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 1 cut(s) 475
ZraI GACGTC 1 cut(s) 402
ZrmI AGTACT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.