MD03G1080600.v1.1

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002651
Physical Location & Seq
Forward (+)
6509776 .. 6512946
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1080600.v1.1.491

Sequence Viewer

Length: 597 bp
ATGTCAGCTTCCGAGGCAACCCACCACCGCCGTTCGGCCACCGGGGGCAGCGGCTCGTCGGTCTCGGATGACTCCGACGATGACCAGTCTTGGCACTCGATGTCTGAGTCGGGCAATGTCCAGAGGAAGTCTTGTGAGTCGGATTGTCCAGTGGAGGAGGTGGATTTGGAGAGTGGGGTTCTGGAGCTGAAGAAGGTGGTGCATTTGAGTAAAGTTGAGAGAAATTGCAGGATTTGCCACTTGGGTTTGGAAGGGGGCGGTGGGACTGACTCTGGCTCTGGGTTCCCAATTGATTTGGGGTGTTCTTGCAAGGGGGATTTGGGCGCTGCTCATAAGCAATGTGCTGAGACTTGGTTCAAGATCAAGGGAGATACAACCTGCGAGATCTGTGGCGTCACTGCATTCAACATTTCCAGTGAGCAGATAAACGAGTCAAATGCCACTACTGCCACATCCGCATCAGTACCAGCAGCACCCATGATCCTGGTTGAAACCCGAACCATGTGGCATGGGCGCCGCATTATGAATTTCCTGCTTGCCTGCATGATCCTTGCCTTTGTAATTTCATGGCTTTTTCACTTCAAAGTGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

199

Amino Acids

21.27

Weight (kDa)

5.43

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 76 - 130 1.2e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 513
AciI CCGC 5 cut(s) 28, 51, 258, 456, 517
AclWI GGATC 2 cut(s) 475, 541
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 2 cut(s) 393, 514
AfaI GTAC 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 4 cut(s) 358, 406, 491, 583
AjnI CCWGG 1 cut(s) 483
AluBI AGCT 2 cut(s) 8, 187
AluI AGCT 2 cut(s) 8, 187
Alw26I GTCTC 2 cut(s) 67, 341
AlwI GGATC 2 cut(s) 475, 541
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 3 cut(s) 48, 326, 470
ApoI RAATTY 1 cut(s) 526
AspLEI GCGC 2 cut(s) 326, 516
AsuC2I CCSGG 1 cut(s) 43
BanI GGYRCC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 60, 313, 482
BceAI ACGGC 1 cut(s) 15
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 485
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 67, 341
BfoI RGCGCY 2 cut(s) 327, 517
BfuAI ACCTGC 1 cut(s) 386
BglII AGATCT 1 cut(s) 384
BisI GCNGC 5 cut(s) 49, 52, 327, 471, 517
BlsI GCNGC 5 cut(s) 50, 53, 328, 472, 518
Bme1390I CCNGG 2 cut(s) 43, 485
BmiI GGNNCC 2 cut(s) 284, 515
BmrFI CCNGG 2 cut(s) 43, 485
BmsI GCATC 1 cut(s) 467
BpmI CTGGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 393, 514
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 85, 149, 414
Bse3DI GCAATG 2 cut(s) 121, 344
BseBI CCWGG 1 cut(s) 485
BseDI CCNNGG 2 cut(s) 12, 42
BseGI GGATG 2 cut(s) 73, 452
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 2 cut(s) 121, 344
BseMII CTCAG 2 cut(s) 96, 336
BseNI ACTGG 3 cut(s) 85, 149, 414
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 3 cut(s) 60, 313, 482
BshFI GGCC 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 513
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 67, 341
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 67
Bsp143I GATC 4 cut(s) 360, 384, 480, 546
BspACI CCGC 5 cut(s) 28, 51, 258, 456, 517
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 2 cut(s) 97, 337
BspHI TCATGA 1 cut(s) 593
BspLI GGNNCC 2 cut(s) 284, 515
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 2 cut(s) 475, 541
BspT107I GGYRCC 1 cut(s) 513
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 121, 344
BsrI ACTGG 3 cut(s) 85, 149, 414
BssECI CCNNGG 2 cut(s) 12, 42
BssMI GATC 4 cut(s) 360, 384, 480, 546
BssNI GRCGYC 2 cut(s) 393, 514
Bst2UI CCWGG 1 cut(s) 485
BstACI GRCGYC 2 cut(s) 393, 514
BstAPI GCANNNNNTGC 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 537, 541
BstDEI CTNAG 2 cut(s) 105, 345
BstF5I GGATG 2 cut(s) 73, 452
BstH2I RGCGCY 2 cut(s) 327, 517
BstHHI GCGC 2 cut(s) 326, 516
BstKTI GATC 4 cut(s) 363, 387, 483, 549
BstMAI GTCTC 2 cut(s) 67, 341
BstMBI GATC 4 cut(s) 360, 384, 480, 546
BstMWI GCNNNNNNNGC 4 cut(s) 14, 234, 446, 455
BstNI CCWGG 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 41, 483
BstV1I GCAGC 3 cut(s) 60, 313, 482
BstX2I RGATCY 1 cut(s) 384
BstXI CCANNNNNNTGG 1 cut(s) 484
BstYI RGATCY 1 cut(s) 384
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 2 cut(s) 73, 452
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 3 cut(s) 156, 396, 421
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 2 cut(s) 537, 541
CciI TCATGA 1 cut(s) 593
CfoI GCGC 2 cut(s) 326, 516
CseI GACGC 1 cut(s) 382
Csp6I GTAC 1 cut(s) 464
CviAII CATG 6 cut(s) 478, 502, 509, 544, 567, 594
CviJI RGCY 6 cut(s) 8, 38, 54, 187, 276, 571
CviKI_1 RGCY 6 cut(s) 8, 38, 54, 187, 276, 571
CviQI GTAC 1 cut(s) 464
DdeI CTNAG 2 cut(s) 105, 345
DinI GGCGCC 1 cut(s) 515
DpnI GATC 4 cut(s) 362, 386, 482, 548
DpnII GATC 4 cut(s) 360, 384, 480, 546
EaeI YGGCCR 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 483
EgeI GGCGCC 1 cut(s) 515
EheI GGCGCC 1 cut(s) 515
FaeI CATG 6 cut(s) 481, 505, 512, 547, 570, 597
FaiI YATR 8 cut(s) 333, 479, 503, 510, 524, 545, 568, 595
FaqI GGGAC 1 cut(s) 277
FatI CATG 6 cut(s) 477, 501, 508, 543, 566, 593
Fnu4HI GCNGC 5 cut(s) 49, 52, 327, 471, 517
FokI GGATG 2 cut(s) 80, 439
Fsp4HI GCNGC 5 cut(s) 49, 52, 327, 471, 517
GlaI GCGC 2 cut(s) 325, 515
GluI GCNGC 5 cut(s) 49, 52, 327, 471, 517
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 2 cut(s) 327, 517
HaeIII GGCC 1 cut(s) 38
HapII CCGG 1 cut(s) 42
HgaI GACGC 1 cut(s) 382
HhaI GCGC 2 cut(s) 326, 516
Hin1I GRCGYC 2 cut(s) 393, 514
Hin1II CATG 6 cut(s) 481, 505, 512, 547, 570, 597
Hin6I GCGC 2 cut(s) 324, 514
HinP1I GCGC 2 cut(s) 324, 514
HinfI GANTC 5 cut(s) 71, 107, 137, 269, 431
HpaII CCGG 1 cut(s) 42
Hpy188I TCNGA 5 cut(s) 13, 67, 76, 106, 142
Hpy188III TCNNGA 4 cut(s) 121, 182, 358, 594
Hpy99I CGWCG 2 cut(s) 61, 80
HpyAV CCTTC 2 cut(s) 187, 245
HpyCH4V TGCA 5 cut(s) 202, 228, 309, 401, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 234, 446, 455
HpyF3I CTNAG 2 cut(s) 105, 345
Hsp92I GRCGYC 2 cut(s) 393, 514
Hsp92II CATG 6 cut(s) 481, 505, 512, 547, 570, 597
HspAI GCGC 2 cut(s) 324, 514
KasI GGCGCC 1 cut(s) 513
Kzo9I GATC 4 cut(s) 360, 384, 480, 546
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 3 cut(s) 60, 313, 482
LweI GCATC 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 394
MalI GATC 4 cut(s) 362, 386, 482, 548
MboI GATC 4 cut(s) 360, 384, 480, 546
MboII GAAGA 1 cut(s) 202
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 1 cut(s) 384
MluCI AATT 4 cut(s) 223, 288, 526, 561
Mly113I GGCGCC 1 cut(s) 514
MlyI GAGTC 5 cut(s) 65, 116, 146, 263, 440
MmeI TCCRAC 2 cut(s) 99, 120
MnlI CCTC 4 cut(s) 7, 117, 148, 151
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 2 cut(s) 43, 485
MunI CAATTG 1 cut(s) 288
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 1 cut(s) 485
MwoI GCNNNNNNNGC 4 cut(s) 14, 234, 446, 455
NarI GGCGCC 1 cut(s) 514
NciI CCSGG 1 cut(s) 43
NdeII GATC 4 cut(s) 360, 384, 480, 546
NlaIII CATG 6 cut(s) 481, 505, 512, 547, 570, 597
NlaIV GGNNCC 2 cut(s) 284, 515
NmuCI GTSAC 1 cut(s) 394
PagI TCATGA 1 cut(s) 593
PctI GAATGC 1 cut(s) 401
PkrI GCNGC 5 cut(s) 50, 53, 328, 472, 518
PleI GAGTC 5 cut(s) 65, 115, 145, 263, 439
PluTI GGCGCC 1 cut(s) 517
PpsI GAGTC 5 cut(s) 65, 115, 145, 263, 439
Psp6I CCWGG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 2 cut(s) 284, 515
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
SatI GCNGC 5 cut(s) 49, 52, 327, 471, 517
Sau3AI GATC 4 cut(s) 360, 384, 480, 546
SchI GAGTC 5 cut(s) 65, 116, 146, 263, 440
ScrFI CCNGG 2 cut(s) 43, 485
SetI ASST 5 cut(s) 10, 162, 189, 198, 380
SfaNI GCATC 1 cut(s) 467
SfoI GGCGCC 1 cut(s) 515
Sse9I AATT 4 cut(s) 223, 288, 526, 561
SsiI CCGC 5 cut(s) 28, 51, 258, 456, 517
SspDI GGCGCC 1 cut(s) 513
StyD4I CCNGG 2 cut(s) 41, 483
TaqI TCGA 1 cut(s) 98
TaqII GACCGA 1 cut(s) 49
TasI AATT 4 cut(s) 223, 288, 526, 561
TauI GCSGC 2 cut(s) 54, 519
TscAI CASTG 3 cut(s) 156, 403, 421
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 3 cut(s) 48, 326, 470
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 539, 555, 582
TspRI CASTG 3 cut(s) 156, 403, 421
XapI RAATTY 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.