Rorug05G0384300

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002651
Physical Location & Seq
Forward (+)
52153930 .. 52154568
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0384300.1

Sequence Viewer

Length: 639 bp
ATGGAACCACAAACCCAGCAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGTAGGCGGAACAGGTGGAATCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTTTAGAGCAGATTCAGAGGATCTGTCTCCAGCTGAAATGGTACGGCCAGATCGAGTTCAAGAACGTCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCGGAAGAAGAGGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGACAATGGTACCAATTGGAAACCTGAGGATCAGTATATTAACTTCGAATCTTCTGCATCTGCTTCTGCTTCTTCAGCTGGTGTGATTGCTTTTAACGGGCAGAAGGGGAACTATGGTGGTTATGGATCTATGAACATGGAGATTGAAACCCTTCCTCTGTTCCCCATGCATGGTGAGGACATCTTTGGTAACATGAAGACTACTTCTGATGGAGGTGGCGGTCACGGTGATGGCTCTCACATTCTTGAGCTCAGCCTTTCCTGTGAGGGCAAAGCTGAAAAAAAAATTTCCGGAGCCGCTGACTCGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

212

Amino Acids

23.42

Weight (kDa)

7.71

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 4.7e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccIII TCCGGA 1 cut(s) 617
AciI CCGC 4 cut(s) 82, 265, 546, 624
AclWI GGATC 3 cut(s) 188, 363, 460
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 2 cut(s) 203, 337
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 2 cut(s) 220, 473
AluBI AGCT 5 cut(s) 130, 193, 404, 577, 602
AluI AGCT 5 cut(s) 130, 193, 404, 577, 602
Alw21I GWGCWC 1 cut(s) 579
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 3 cut(s) 188, 363, 460
Ama87I CYCGRG 1 cut(s) 255
Aor13HI TCCGGA 1 cut(s) 617
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 477
Asp718I GGTACC 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 153, 276
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 335, 512, 566
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 2 cut(s) 153, 276
AxyI CCTNAGG 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 2 cut(s) 256, 579
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 579
BbvI GCAGC 1 cut(s) 62
BccI CCATC 3 cut(s) 26, 530, 551
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 102
BisI GCNGC 2 cut(s) 51, 624
BlpI GCTNAGC 1 cut(s) 578
BlsI GCNGC 2 cut(s) 52, 625
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 2 cut(s) 153, 276
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 153, 276
BmiI GGNNCC 4 cut(s) 6, 155, 337, 622
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 173
Bpu1102I GCTNAGC 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 107, 593
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 617
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse21I CCTNAGG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 37, 292
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 2 cut(s) 342, 592
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 15
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 2 cut(s) 54, 618
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 497
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 256, 300, 579
Bsp13I TCCGGA 1 cut(s) 617
Bsp143I GATC 6 cut(s) 106, 180, 210, 308, 355, 452
Bsp1720I GCTNAGC 1 cut(s) 578
BspACI CCGC 4 cut(s) 82, 265, 546, 624
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 2 cut(s) 343, 591
BspEI TCCGGA 1 cut(s) 617
BspLI GGNNCC 4 cut(s) 6, 155, 337, 622
BspPI GGATC 3 cut(s) 188, 363, 460
BspT104I TTCGAA 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 6 cut(s) 106, 180, 210, 308, 355, 452
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 1 cut(s) 266
BstBI TTCGAA 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 351, 578, 636
BstF5I GGATG 2 cut(s) 37, 292
BstKTI GATC 6 cut(s) 109, 183, 213, 311, 358, 455
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 6 cut(s) 106, 180, 210, 308, 355, 452
BstMWI GCNNNNNNNGC 3 cut(s) 79, 401, 632
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 53
BstSFI CTRYAG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 180, 452
BstYI RGATCY 2 cut(s) 180, 452
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 37, 292
Cfr13I GGNCC 2 cut(s) 153, 276
Csp6I GTAC 2 cut(s) 202, 336
CviAII CATG 7 cut(s) 70, 293, 301, 463, 493, 497, 520
CviQI GTAC 2 cut(s) 202, 336
DdeI CTNAG 3 cut(s) 351, 578, 636
DpnI GATC 6 cut(s) 108, 182, 212, 310, 357, 454
DpnII GATC 6 cut(s) 106, 180, 210, 308, 355, 452
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 577
Eco24I GRGCYC 2 cut(s) 256, 579
Eco47I GGWCC 2 cut(s) 153, 276
Eco53kI GAGCTC 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 2 cut(s) 256, 579
FaeI CATG 7 cut(s) 73, 296, 304, 466, 496, 500, 523
FaqI GGGAC 1 cut(s) 139
FatI CATG 7 cut(s) 69, 292, 300, 462, 492, 496, 519
Fnu4HI GCNGC 2 cut(s) 51, 624
FokI GGATG 2 cut(s) 44, 299
FriOI GRGCYC 2 cut(s) 256, 579
Fsp4HI GCNGC 2 cut(s) 51, 624
GluI GCNGC 2 cut(s) 51, 624
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 173
HaeIII GGCC 1 cut(s) 207
HapII CCGG 2 cut(s) 54, 618
Hin1II CATG 7 cut(s) 73, 296, 304, 466, 496, 500, 523
HinfI GANTC 5 cut(s) 94, 117, 172, 374, 629
HpaII CCGG 2 cut(s) 54, 618
HphI GGTGA 3 cut(s) 335, 512, 566
Hpy166II GTNNAC 2 cut(s) 244, 279
Hpy188I TCNGA 4 cut(s) 111, 177, 286, 535
Hpy188III TCNNGA 5 cut(s) 220, 241, 257, 572, 618
Hpy8I GTNNAC 2 cut(s) 244, 279
HpyAV CCTTC 2 cut(s) 424, 488
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 383, 496
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 401, 632
HpyF3I CTNAG 3 cut(s) 351, 578, 636
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 7 cut(s) 73, 296, 304, 466, 496, 500, 523
Kpn2I TCCGGA 1 cut(s) 617
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 6 cut(s) 106, 180, 210, 308, 355, 452
LmnI GCTCC 2 cut(s) 127, 620
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 392
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 515, 548
MalI GATC 6 cut(s) 108, 182, 212, 310, 357, 454
MboI GATC 6 cut(s) 106, 180, 210, 308, 355, 452
MboII GAAGA 5 cut(s) 280, 283, 369, 390, 535
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 2 cut(s) 180, 452
MhlI GDGCHC 3 cut(s) 256, 300, 579
MluCI AATT 2 cut(s) 340, 612
MlyI GAGTC 1 cut(s) 623
Mph1103I ATGCAT 1 cut(s) 498
MroI TCCGGA 1 cut(s) 617
MroXI GAANNNNTTC 1 cut(s) 477
MseI TTAA 2 cut(s) 366, 420
MslI CAYNNNNRTG 1 cut(s) 555
MspA1I CMGCKG 3 cut(s) 193, 404, 626
MspI CCGG 2 cut(s) 54, 618
MspR9I CCNGG 1 cut(s) 55
MunI CAATTG 1 cut(s) 340
MwoI GCNNNNNNNGC 3 cut(s) 79, 401, 632
NciI CCSGG 1 cut(s) 55
NdeII GATC 6 cut(s) 106, 180, 210, 308, 355, 452
NlaIII CATG 7 cut(s) 73, 296, 304, 466, 496, 500, 523
NlaIV GGNNCC 4 cut(s) 6, 155, 337, 622
NmeAIII GCCGAG 1 cut(s) 611
NmuCI GTSAC 1 cut(s) 548
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 1 cut(s) 73
NspV TTCGAA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 477
PfeI GAWTC 4 cut(s) 94, 117, 172, 374
PkrI GCNGC 2 cut(s) 52, 625
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 579
Psp5II RGGWCCY 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 4 cut(s) 6, 155, 337, 622
PspPI GGNCC 2 cut(s) 153, 276
PspPPI RGGWCCY 1 cut(s) 153
PsuI RGATCY 2 cut(s) 180, 452
PvuII CAGCTG 2 cut(s) 193, 404
RsaI GTAC 2 cut(s) 203, 337
RsaNI GTAC 2 cut(s) 202, 336
RseI CAYNNNNRTG 1 cut(s) 555
SacI GAGCTC 1 cut(s) 579
SaqAI TTAA 2 cut(s) 366, 420
SatI GCNGC 2 cut(s) 51, 624
Sau3AI GATC 6 cut(s) 106, 180, 210, 308, 355, 452
Sau96I GGNCC 2 cut(s) 153, 276
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 3 cut(s) 256, 300, 579
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 2 cut(s) 153, 276
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 2 cut(s) 122, 572
SmoI CTYRAG 2 cut(s) 122, 572
Sse9I AATT 2 cut(s) 340, 612
SsiI CCGC 4 cut(s) 82, 265, 546, 624
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 1 cut(s) 228
TaqI TCGA 2 cut(s) 213, 372
TasI AATT 2 cut(s) 340, 612
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 4 cut(s) 94, 117, 172, 374
Tru1I TTAA 2 cut(s) 366, 420
Tru9I TTAA 2 cut(s) 366, 420
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 3 cut(s) 281, 473, 536
VpaK11BI GGWCC 2 cut(s) 153, 276
XapI RAATTY 1 cut(s) 612
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.