Rh5BG460700

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002651
Physical Location & Seq
Reverse (-)
73644905 .. 73647761
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG460700.1

Sequence Viewer

Length: 558 bp
ATGATGTTGGCTGTTGTTGAAGCTGCGCAGAGTCACAGGCATTCTGTGAGTGACGTGTCCGTTTCGGATGAGTCCACGTTTGATTCTTGTGGGTCGGATAGTTGTTCTTTGGAGGGTGTGGATTTGGAGAGTGGGGTTTTGGAGCTGAAGAATAAGGTGGTGGTGCATTTGAGTAAGGTTGAGAGAATTTGCAGAATTTGCCACTTGGGTTTTGAGGGTGGTGGGTTGGACTCTTCTGGGCTTCCAATTGACTTGGGATGTTCTTGCAAGGGTGATTTGGGTGCTGCTCATAAGCAATGTGCTGAGACTTGGTTCAAGATCAAGGGTGATACAATCTGCGAGATCTGTGGTGTCGTTGCATTTAACATAGTTGGTGAGCAGATCAACGAGGCAAATGGAGCCATCGCCCCAACATCCCCTTCAACACAACCAGCAGCATCTGTGATCATGGTGGAAACCCAAACCATGTGGCATGGCCGCCGCATTATGAATTTCCTGCTTGCTTGCATGATCATTGCCTTTGTTATTTCATGGCTTTTCCACTTCAAAATACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

185

Amino Acids

19.87

Weight (kDa)

5.26

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 64 - 116 3e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 27
AciI CCGC 2 cut(s) 478, 481
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 3 cut(s) 186, 195, 490
AcuI CTGAAG 1 cut(s) 167
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 20, 316, 423, 547
AjiI CACGTC 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AluBI AGCT 2 cut(s) 23, 145
AluI AGCT 2 cut(s) 23, 145
Alw26I GTCTC 1 cut(s) 299
AlwNI CAGNNNCTG 1 cut(s) 440
AoxI GGCC 1 cut(s) 475
ApeKI GCWGC 3 cut(s) 23, 284, 434
ApoI RAATTY 3 cut(s) 186, 195, 490
AspLEI GCGC 1 cut(s) 28
AsuHPI GGTGA 3 cut(s) 284, 338, 386
BbvI GCAGC 3 cut(s) 10, 271, 446
BccI CCATC 1 cut(s) 410
BclI TGATCA 2 cut(s) 444, 510
BcoDI GTCTC 1 cut(s) 299
BglII AGATCT 1 cut(s) 342
BisI GCNGC 5 cut(s) 24, 285, 435, 478, 481
BlsI GCNGC 5 cut(s) 25, 286, 436, 479, 482
BmgBI CACGTC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 400
BmsI GCATC 1 cut(s) 446
Bse3DI GCAATG 2 cut(s) 302, 513
BseGI GGATG 3 cut(s) 73, 263, 413
BseMI GCAATG 2 cut(s) 302, 513
BseMII CTCAG 1 cut(s) 294
BseXI GCAGC 3 cut(s) 10, 271, 446
BshFI GGCC 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 1 cut(s) 477
Bsp143I GATC 5 cut(s) 318, 342, 381, 444, 510
BspACI CCGC 2 cut(s) 478, 481
BspANI GGCC 1 cut(s) 477
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 302, 513
BssMI GATC 5 cut(s) 318, 342, 381, 444, 510
Bst6I CTCTTC 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 501, 505
BstDEI CTNAG 1 cut(s) 303
BstF5I GGATG 3 cut(s) 73, 263, 413
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 5 cut(s) 321, 345, 384, 447, 513
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 5 cut(s) 318, 342, 381, 444, 510
BstMWI GCNNNNNNNGC 2 cut(s) 198, 398
BstV1I GCAGC 3 cut(s) 10, 271, 446
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BsuRI GGCC 1 cut(s) 477
BtgZI GCGATG 1 cut(s) 388
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 3 cut(s) 73, 263, 413
Cac8I GCNNGC 2 cut(s) 501, 505
CaiI CAGNNNCTG 1 cut(s) 440
CfoI GCGC 1 cut(s) 28
CspCI CAANNNNNGTGG 2 cut(s) 449, 484
CviAII CATG 5 cut(s) 448, 466, 473, 508, 531
CviJI RGCY 7 cut(s) 11, 23, 145, 241, 401, 477, 535
CviKI_1 RGCY 7 cut(s) 11, 23, 145, 241, 401, 477, 535
DdeI CTNAG 1 cut(s) 303
DpnI GATC 5 cut(s) 320, 344, 383, 446, 512
DpnII GATC 5 cut(s) 318, 342, 381, 444, 510
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 167
FaeI CATG 5 cut(s) 451, 469, 476, 511, 534
FaiI YATR 9 cut(s) 291, 368, 449, 467, 474, 488, 509, 532, 556
FatI CATG 5 cut(s) 447, 465, 472, 507, 530
FbaI TGATCA 2 cut(s) 444, 510
Fnu4HI GCNGC 5 cut(s) 24, 285, 435, 478, 481
FokI GGATG 3 cut(s) 80, 270, 400
Fsp4HI GCNGC 5 cut(s) 24, 285, 435, 478, 481
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 1 cut(s) 27
GluI GCNGC 5 cut(s) 24, 285, 435, 478, 481
HaeIII GGCC 1 cut(s) 477
HhaI GCGC 1 cut(s) 28
Hin1II CATG 5 cut(s) 451, 469, 476, 511, 534
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HinfI GANTC 4 cut(s) 31, 71, 83, 230
HphI GGTGA 3 cut(s) 284, 338, 386
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 2 cut(s) 67, 97
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 1 cut(s) 429
HpyCH4IV ACGT 2 cut(s) 54, 77
HpyCH4V TGCA 5 cut(s) 166, 192, 267, 359, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 398
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 2 cut(s) 54, 77
Hsp92II CATG 5 cut(s) 451, 469, 476, 511, 534
HspAI GCGC 1 cut(s) 26
Ksp22I TGATCA 2 cut(s) 444, 510
Kzo9I GATC 5 cut(s) 318, 342, 381, 444, 510
LmnI GCTCC 2 cut(s) 142, 398
LpnPI CCDG 4 cut(s) 22, 222, 444, 509
Lsp1109I GCAGC 3 cut(s) 10, 271, 446
LweI GCATC 1 cut(s) 446
MaeII ACGT 2 cut(s) 54, 77
MaeIII GTNAC 2 cut(s) 32, 50
MalI GATC 5 cut(s) 320, 344, 383, 446, 512
MboI GATC 5 cut(s) 318, 342, 381, 444, 510
MboII GAAGA 2 cut(s) 160, 225
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 342
MluCI AATT 4 cut(s) 186, 195, 246, 490
MlyI GAGTC 3 cut(s) 40, 80, 224
MmeI TCCRAC 2 cut(s) 75, 207
MnlI CCTC 3 cut(s) 106, 208, 382
MseI TTAA 1 cut(s) 363
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 198, 398
NdeII GATC 5 cut(s) 318, 342, 381, 444, 510
NlaIII CATG 5 cut(s) 451, 469, 476, 511, 534
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 32, 50
NsbI TGCGCA 1 cut(s) 27
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 5 cut(s) 25, 286, 436, 479, 482
PleI GAGTC 3 cut(s) 39, 79, 224
PpsI GAGTC 3 cut(s) 39, 79, 224
PspN4I GGNNCC 1 cut(s) 400
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 342
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 5 cut(s) 24, 285, 435, 478, 481
Sau3AI GATC 5 cut(s) 318, 342, 381, 444, 510
SchI GAGTC 3 cut(s) 40, 80, 224
SetI ASST 6 cut(s) 25, 57, 80, 147, 159, 180
SfaNI GCATC 1 cut(s) 446
Sse9I AATT 4 cut(s) 186, 195, 246, 490
SsiI CCGC 2 cut(s) 478, 481
TaiI ACGT 2 cut(s) 57, 80
TasI AATT 4 cut(s) 186, 195, 246, 490
TauI GCSGC 2 cut(s) 480, 483
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseFI GTSAC 2 cut(s) 32, 50
TseI GCWGC 3 cut(s) 23, 284, 434
Tsp45I GTSAC 2 cut(s) 32, 50
TspDTI ATGAA 2 cut(s) 503, 519
TspGWI ACGGA 1 cut(s) 49
XapI RAATTY 3 cut(s) 186, 195, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.