FvH4_3g40272

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001631
Physical Location & Seq
Forward (+)
34026333 .. 34027798
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40272.t1

Sequence Viewer

Length: 1017 bp
ATGAATAAATCGACGATGAAATATATCAAAGCAATTATATATGTAAGCAGAGGAAATAATGAGCCATTCAGTGTTCCGGCTCCTAAAATGGACCATGAATATTCATACACCACTCTCACCATAGAGAAAATATTGAGTATCGTAAATTTCAAGCTCAAGACGCGCGAGATGTACTTCTTCTGGAAGACGCTGTTTGAGGAGATCTTCAAGCGTTACAAGCTCACCGACATTGTCGACGGGAGCGAACCCTGTCCTGACAGGTTCCTGATCGATATGAATGGAAACATCACGGATCAAGCCGATCCCGCCTTTGATGTTTGGCACGAGAAGGATCAGAAGATCATAATGTGGATCAAGTCGACTCTTTCCGACGACGTGGCCCTCCCACTAGTCATAAACGGAGACATCCCTTCCAATTCCAGTCGCGAGCTATGGCTTGGCCTCGAGAAGAGATTCGCAGTTGCCGGGGTGTCAGCCGAGTATAGGAAAAGTATGTTCGCCGCCGCCTTGGCGACCACGGACATTTGTGACGCAAATCCCGCTCTGTTGAAGAGTGGGGACTTGCGTCTTTTGGCGCCAATTTTTCAAAAATTTGGAAAATGGCGCAGGTTTTCGGGTCGTGTTGTGACCCTAAAGGTGTTTGAGGACAACACTTTGGTGTTGGAGGCTTTGTCGAGACAAGGGGAGGGGAGGGTGTTGGTTGTGGACGGCGGAGGAAGCATGAGACGTGCCGTCTCCGGAGGGATGGTGGCAAAGTATGCGGAGTTGATGGGGTGGGCTGGGATTGTTGTGAATGGATGCGTCAGAGATGTGGATGAGATCAATGAATGTGAGATCGGCGTGAGAGCTTTGGCGACTTGTCCGGTGAGACCTACTAAAGGTAAGGATGGAAGCAGTGGACAGAAGCATGTTGATGTCAACATTGGAGGAACATGGATTCTTGATGGAGAATGGTTGTATGCAGATAATGATGGAATCCTTGTCTCCACGACTAAATTTTCATATCTCTTAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

339

Amino Acids

37.78

Weight (kDa)

6.37

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 172 - 328 6.4e-42 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 597
AccB1I GGYRCC 1 cut(s) 574
AccBSI CCGCTC 1 cut(s) 542
AccI GTMKAC 2 cut(s) 234, 359
AccII CGCG 3 cut(s) 163, 165, 426
AccIII TCCGGA 1 cut(s) 737
AciI CCGC 6 cut(s) 306, 501, 504, 540, 711, 761
AclWI GGATC 4 cut(s) 296, 300, 339, 359
AcsI RAATTY 3 cut(s) 145, 590, 995
AcyI GRCGYC 1 cut(s) 575
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 483, 878
AgsI TTSAA 4 cut(s) 151, 208, 550, 587
AhdI GACNNNNNGTC 1 cut(s) 731
AhlI ACTAGT 1 cut(s) 388
AjiI CACGTC 2 cut(s) 376, 728
AleI CACNNNNGTG 1 cut(s) 656
AluBI AGCT 4 cut(s) 154, 220, 430, 848
AluI AGCT 4 cut(s) 154, 220, 430, 848
Alw26I GTCTC 6 cut(s) 396, 670, 718, 739, 862, 988
AlwI GGATC 4 cut(s) 296, 300, 339, 359
Ama87I CYCGRG 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 737
AoxI GGCC 2 cut(s) 378, 439
ApoI RAATTY 3 cut(s) 145, 590, 995
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 3 cut(s) 165, 577, 606
AspS9I GGNCC 2 cut(s) 91, 379
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 3 cut(s) 109, 214, 877
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 574
BauI CACGAG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 191
BccI CCATC 5 cut(s) 739, 763, 881, 938, 965
BceAI ACGGC 2 cut(s) 716, 724
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 6 cut(s) 396, 670, 718, 739, 862, 988
BcuI ACTAGT 1 cut(s) 388
BfaI CTAG 1 cut(s) 389
BfoI RGCGCY 1 cut(s) 578
BfuAI ACCTGC 1 cut(s) 597
BglI GCCNNNNNGGC 1 cut(s) 509
BglII AGATCT 1 cut(s) 201
BisI GCNGC 2 cut(s) 501, 504
BlsI GCNGC 2 cut(s) 502, 505
Bme1390I CCNGG 1 cut(s) 466
Bme18I GGWCC 1 cut(s) 91
BmeRI GACNNNNNGTC 1 cut(s) 731
BmeT110I CYCGRG 1 cut(s) 443
BmgBI CACGTC 2 cut(s) 376, 728
BmgT120I GGNCC 2 cut(s) 91, 379
BmiI GGNNCC 3 cut(s) 81, 263, 576
BmrFI CCNGG 1 cut(s) 466
BmsI GCATC 1 cut(s) 788
BoxI GACNNNNGTC 1 cut(s) 564
BpiI GAAGAC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 575
BsaI GGTCTC 1 cut(s) 862
BsaJI CCNNGG 3 cut(s) 465, 507, 516
BsaWI WCCGGW 2 cut(s) 737, 862
BsaXI ACNNNNNCTCC 4 cut(s) 656, 686, 732, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 878
Bse1I ACTGG 1 cut(s) 420
BseAI TCCGGA 1 cut(s) 737
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 3 cut(s) 465, 507, 516
BseGI GGATG 5 cut(s) 405, 750, 803, 820, 892
BseLI CCNNNNNNNGG 2 cut(s) 483, 878
BseNI ACTGG 1 cut(s) 420
BseRI GAGGAG 1 cut(s) 212
BseYI CCCAGC 1 cut(s) 779
Bsh1236I CGCG 3 cut(s) 163, 165, 426
BshFI GGCC 2 cut(s) 380, 441
BshNI GGYRCC 1 cut(s) 574
BshVI ATCGAT 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 4 cut(s) 77, 465, 738, 863
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 2 cut(s) 483, 878
BsmAI GTCTC 6 cut(s) 396, 670, 718, 739, 862, 988
BsmBI CGTCTC 2 cut(s) 718, 739
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 2 cut(s) 380, 441
Bso31I GGTCTC 1 cut(s) 862
BsoBI CYCGRG 1 cut(s) 443
Bsp13I TCCGGA 1 cut(s) 737
Bsp143I GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
Bsp68I TCGCGA 1 cut(s) 426
BspACI CCGC 6 cut(s) 306, 501, 504, 540, 711, 761
BspANI GGCC 2 cut(s) 380, 441
BspDI ATCGAT 1 cut(s) 270
BspEI TCCGGA 1 cut(s) 737
BspFNI CGCG 3 cut(s) 163, 165, 426
BspLI GGNNCC 3 cut(s) 81, 263, 576
BspMI ACCTGC 1 cut(s) 597
BspPI GGATC 4 cut(s) 296, 300, 339, 359
BspT107I GGYRCC 1 cut(s) 574
BspTNI GGTCTC 1 cut(s) 862
BsrBI CCGCTC 1 cut(s) 542
BsrI ACTGG 1 cut(s) 420
BssECI CCNNGG 3 cut(s) 465, 507, 516
BssMI GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
BssNI GRCGYC 1 cut(s) 575
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 443, 545
BstACI GRCGYC 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 758
BstC8I GCNNGC 1 cut(s) 428
BstDEI CTNAG 1 cut(s) 1009
BstDSI CCRYGG 1 cut(s) 516
BstENI CCTNNNNNAGG 1 cut(s) 876
BstF5I GGATG 5 cut(s) 405, 750, 803, 820, 892
BstFNI CGCG 3 cut(s) 163, 165, 426
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 3 cut(s) 165, 577, 606
BstKTI GATC 9 cut(s) 204, 270, 295, 304, 334, 342, 354, 822, 837
BstMAI GTCTC 6 cut(s) 396, 670, 718, 739, 862, 988
BstMBI GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
BstMWI GCNNNNNNNGC 7 cut(s) 160, 217, 305, 509, 539, 717, 758
BstNSI RCATGY 1 cut(s) 911
BstPAI GACNNNNGTC 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 464
BstUI CGCG 3 cut(s) 163, 165, 426
BstV2I GAAGAC 1 cut(s) 191
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 2 cut(s) 380, 441
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 516
BtrI CACGTC 2 cut(s) 376, 728
BtsCI GGATG 5 cut(s) 405, 750, 803, 820, 892
BtsI GCAGTG 1 cut(s) 901
BtsIMutI CAGTG 2 cut(s) 76, 901
BtuMI TCGCGA 1 cut(s) 426
BveI ACCTGC 1 cut(s) 597
Cac8I GCNNGC 1 cut(s) 428
CfoI GCGC 3 cut(s) 165, 577, 606
Cfr13I GGNCC 2 cut(s) 91, 379
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 5 cut(s) 169, 196, 539, 554, 790
Csp6I GTAC 1 cut(s) 172
CviAII CATG 4 cut(s) 95, 721, 908, 933
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 1009
DinI GGCGCC 1 cut(s) 576
DpnI GATC 9 cut(s) 203, 269, 294, 303, 333, 341, 353, 821, 836
DpnII GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
DriI GACNNNNNGTC 1 cut(s) 731
Eam1104I CTCTTC 2 cut(s) 443, 545
Eam1105I GACNNNNNGTC 1 cut(s) 731
EarI CTCTTC 2 cut(s) 443, 545
EciI GGCGGA 1 cut(s) 726
Eco130I CCWWGG 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 862
Eco47I GGWCC 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 443
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoT14I CCWWGG 1 cut(s) 507
EgeI GGCGCC 1 cut(s) 576
EheI GGCGCC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 507
Esp3I CGTCTC 2 cut(s) 718, 739
FaeI CATG 4 cut(s) 98, 724, 911, 936
FaqI GGGAC 1 cut(s) 572
FatI CATG 4 cut(s) 94, 720, 907, 932
FauI CCCGC 2 cut(s) 313, 547
FblI GTMKAC 2 cut(s) 234, 359
Fnu4HI GCNGC 2 cut(s) 501, 504
FokI GGATG 5 cut(s) 392, 757, 810, 827, 899
Fsp4HI GCNGC 2 cut(s) 501, 504
FspBI CTAG 1 cut(s) 389
GlaI GCGC 3 cut(s) 164, 576, 605
GluI GCNGC 2 cut(s) 501, 504
GsaI CCCAGC 1 cut(s) 783
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 2 cut(s) 380, 441
HapII CCGG 4 cut(s) 77, 465, 738, 863
HgaI GACGC 5 cut(s) 169, 196, 539, 554, 790
HhaI GCGC 3 cut(s) 165, 577, 606
Hin1I GRCGYC 1 cut(s) 575
Hin1II CATG 4 cut(s) 98, 724, 911, 936
Hin6I GCGC 3 cut(s) 163, 575, 604
HinP1I GCGC 3 cut(s) 163, 575, 604
HincII GTYRAC 3 cut(s) 235, 360, 919
HindII GTYRAC 3 cut(s) 235, 360, 919
HinfI GANTC 4 cut(s) 361, 453, 937, 975
HpaII CCGG 4 cut(s) 77, 465, 738, 863
HphI GGTGA 3 cut(s) 109, 214, 877
Hpy166II GTNNAC 5 cut(s) 235, 360, 706, 899, 919
Hpy188I TCNGA 3 cut(s) 336, 370, 806
Hpy188III TCNNGA 9 cut(s) 157, 181, 254, 265, 425, 445, 675, 738, 941
Hpy8I GTNNAC 5 cut(s) 235, 360, 706, 899, 919
Hpy99I CGWCG 4 cut(s) 16, 239, 374, 377
HpyAV CCTTC 2 cut(s) 322, 420
HpyCH4IV ACGT 2 cut(s) 375, 727
HpyCH4V TGCA 1 cut(s) 962
HpyF10VI GCNNNNNNNGC 7 cut(s) 160, 217, 305, 509, 539, 717, 758
HpyF3I CTNAG 1 cut(s) 1009
HpySE526I ACGT 2 cut(s) 375, 727
Hsp92I GRCGYC 1 cut(s) 575
Hsp92II CATG 4 cut(s) 98, 724, 911, 936
HspAI GCGC 3 cut(s) 163, 575, 604
KasI GGCGCC 1 cut(s) 574
Kpn2I TCCGGA 1 cut(s) 737
Kzo9I GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
LmnI GCTCC 2 cut(s) 85, 240
LweI GCATC 1 cut(s) 788
MaeI CTAG 1 cut(s) 389
MaeII ACGT 2 cut(s) 375, 727
MaeIII GTNAC 3 cut(s) 212, 527, 625
MalI GATC 9 cut(s) 203, 269, 294, 303, 333, 341, 353, 821, 836
MbiI CCGCTC 1 cut(s) 542
MboI GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
MboII GAAGA 6 cut(s) 169, 196, 196, 349, 460, 562
MflI RGATCY 1 cut(s) 201
MluCI AATT 6 cut(s) 33, 145, 415, 579, 590, 995
Mly113I GGCGCC 1 cut(s) 575
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 2 cut(s) 393, 642
MroI TCCGGA 1 cut(s) 737
MroXI GAANNNNTTC 1 cut(s) 452
MslI CAYNNNNRTG 2 cut(s) 656, 912
MspI CCGG 4 cut(s) 77, 465, 738, 863
MspR9I CCNGG 1 cut(s) 466
MvnI CGCG 3 cut(s) 163, 165, 426
MwoI GCNNNNNNNGC 7 cut(s) 160, 217, 305, 509, 539, 717, 758
NarI GGCGCC 1 cut(s) 575
NciI CCSGG 1 cut(s) 466
NdeII GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
NlaIII CATG 4 cut(s) 98, 724, 911, 936
NlaIV GGNNCC 3 cut(s) 81, 263, 576
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 2 cut(s) 527, 625
NruI TCGCGA 1 cut(s) 426
NspI RCATGY 1 cut(s) 911
OliI CACNNNNGTG 1 cut(s) 656
PaeR7I CTCGAG 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 3 cut(s) 453, 937, 975
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 2 cut(s) 502, 505
PleI GAGTC 1 cut(s) 355
PluTI GGCGCC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 355
PshAI GACNNNNGTC 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 779
PspN4I GGNNCC 3 cut(s) 81, 263, 576
PspPI GGNCC 2 cut(s) 91, 379
PsuI RGATCY 1 cut(s) 201
PsyI GACNNNGTC 1 cut(s) 230
RruI TCGCGA 1 cut(s) 426
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 656, 912
SalI GTCGAC 2 cut(s) 233, 358
SatI GCNGC 2 cut(s) 501, 504
Sau3AI GATC 9 cut(s) 201, 267, 292, 301, 331, 339, 351, 819, 834
Sau96I GGNCC 2 cut(s) 91, 379
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 1 cut(s) 466
SfaNI GCATC 1 cut(s) 788
SfoI GGCGCC 1 cut(s) 576
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 1 cut(s) 91
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 2 cut(s) 656, 912
SmlI CTYRAG 2 cut(s) 155, 443
SmoI CTYRAG 2 cut(s) 155, 443
SpeI ACTAGT 1 cut(s) 388
Sse9I AATT 6 cut(s) 33, 145, 415, 579, 590, 995
SsiI CCGC 6 cut(s) 306, 501, 504, 540, 711, 761
SspDI GGCGCC 1 cut(s) 574
SspI AATATT 2 cut(s) 101, 132
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 464
StyI CCWWGG 1 cut(s) 507
TaiI ACGT 2 cut(s) 378, 730
TaqI TCGA 6 cut(s) 11, 234, 270, 359, 444, 674
TasI AATT 6 cut(s) 33, 145, 415, 579, 590, 995
TatI WGTACW 1 cut(s) 171
TauI GCSGC 2 cut(s) 503, 506
TfiI GAWTC 3 cut(s) 453, 937, 975
TscAI CASTG 2 cut(s) 76, 901
TseFI GTSAC 2 cut(s) 527, 625
Tsp45I GTSAC 2 cut(s) 527, 625
TspDTI ATGAA 7 cut(s) 17, 32, 93, 111, 290, 840, 990
TspGWI ACGGA 3 cut(s) 305, 414, 533
TspRI CASTG 2 cut(s) 76, 901
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 91
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 3 cut(s) 145, 590, 995
XceI RCATGY 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 443
XmiI GTMKAC 2 cut(s) 234, 359
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.