FvH4_3g41390

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001631
Physical Location & Seq
Reverse (-)
34764547 .. 34767775
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41390.t4

Sequence Viewer

Length: 501 bp
ATGGCTTTCTTAGCAACTGCTGAAGTTTGTGACACAAATACAGCCCTTTTGGGAAGTGGAGACTTGCGCGTCCTGCCACCAATGTTCCAGATGTATGGACAATGTCGAGCATTCTCAGGCCCCATTTCCACTATTAAAGTTTTTGAGGACAATGTACTGGTTAGGGAGCTTCTTGAAACCAGAGGCGAGGGAAGAGTATTGGTCATTGATGGTGGAGGAAGCATGAGATGTGCTCTGGTTGGAGGGAATATGGGACAGTTGGCTCAAAACATGGGATGGGCTGGTATTGTGGTAAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTTAGGGCCTTGGCATCTCATCCCATGAAGTCAAACAAAAGAGGCCATGGCGATAAGCATGTTCCAATCAACATTGCAGGAACCTTGATCCGCGAAGGGGAATGGTTGTATGCTGATAGTGATGGCATCCTTGTCTCAACATCTGAATTGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.69

Weight (kDa)

5.12

Isoelectric Point (pI)

19.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 85 - 238 7.4e-46 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccII CGCG 2 cut(s) 69, 435
AciI CCGC 1 cut(s) 433
AclWI GGATC 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 1 cut(s) 176
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw21I GWGCWC 1 cut(s) 235
Alw26I GTCTC 2 cut(s) 54, 481
AlwI GGATC 1 cut(s) 424
AoxI GGCC 3 cut(s) 118, 348, 385
ApeKI GCWGC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 119, 348
Bbv12I GWGCWC 1 cut(s) 235
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 203, 270, 458
BcoDI GTCTC 2 cut(s) 54, 481
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 119, 348
BmiI GGNNCC 2 cut(s) 121, 424
BmsI GCATC 2 cut(s) 365, 477
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BsaJI CCNNGG 2 cut(s) 351, 388
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 162
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 351, 388
BseGI GGATG 4 cut(s) 281, 335, 361, 468
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 287
Bsh1236I CGCG 2 cut(s) 69, 435
BshFI GGCC 3 cut(s) 120, 350, 387
BsiHKAI GWGCWC 1 cut(s) 235
BslFI GGGAC 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 54, 481
BsmFI GGGAC 1 cut(s) 267
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 3 cut(s) 120, 350, 387
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 2 cut(s) 321, 429
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 1 cut(s) 433
BspANI GGCC 3 cut(s) 120, 350, 387
BspCNI CTCAG 1 cut(s) 128
BspFNI CGCG 2 cut(s) 69, 435
BspLI GGNNCC 2 cut(s) 121, 424
BspPI GGATC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 2 cut(s) 351, 388
BssMI GATC 2 cut(s) 321, 429
BssT1I CCWWGG 2 cut(s) 351, 388
Bst4CI ACNGT 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 10, 115
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 4 cut(s) 281, 335, 361, 468
BstFNI CGCG 2 cut(s) 69, 435
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 2 cut(s) 324, 432
BstMAI GTCTC 2 cut(s) 54, 481
BstMBI GATC 2 cut(s) 321, 429
BstMWI GCNNNNNNNGC 2 cut(s) 11, 73
BstNSI RCATGY 1 cut(s) 404
BstUI CGCG 2 cut(s) 69, 435
BstV1I GCAGC 1 cut(s) 287
BstXI CCANNNNNNTGG 1 cut(s) 95
BsuRI GGCC 3 cut(s) 120, 350, 387
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 4 cut(s) 281, 335, 361, 468
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 119, 348
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 155
CviAII CATG 5 cut(s) 223, 271, 367, 389, 401
CviJI RGCY 9 cut(s) 5, 44, 120, 169, 263, 281, 300, 350, 387
CviKI_1 RGCY 9 cut(s) 5, 44, 120, 169, 263, 281, 300, 350, 387
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 2 cut(s) 10, 115
DpnI GATC 2 cut(s) 323, 431
DpnII GATC 2 cut(s) 321, 429
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 351, 388
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 2 cut(s) 119, 348
EcoT14I CCWWGG 2 cut(s) 351, 388
ErhI CCWWGG 2 cut(s) 351, 388
FaeI CATG 5 cut(s) 226, 274, 370, 392, 404
FaiI YATR 8 cut(s) 96, 224, 251, 272, 368, 390, 402, 453
FaqI GGGAC 1 cut(s) 267
FatI CATG 5 cut(s) 222, 270, 366, 388, 400
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 4 cut(s) 288, 342, 348, 455
Fsp4HI GCNGC 1 cut(s) 301
GlaI GCGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 3 cut(s) 120, 350, 387
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 69
Hin1II CATG 5 cut(s) 226, 274, 370, 392, 404
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 2 cut(s) 88, 173
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 303, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 73
HpyF3I CTNAG 2 cut(s) 10, 115
Hsp92II CATG 5 cut(s) 226, 274, 370, 392, 404
HspAI GCGC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 321, 429
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 8 cut(s) 86, 101, 102, 143, 193, 221, 267, 405
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 2 cut(s) 365, 477
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 323, 431
MboI GATC 2 cut(s) 321, 429
MboII GAAGA 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 6 cut(s) 139, 176, 181, 209, 236, 377
MseI TTAA 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 110
MvnI CGCG 2 cut(s) 69, 435
MwoI GCNNNNNNNGC 2 cut(s) 11, 73
NcoI CCATGG 1 cut(s) 388
NdeII GATC 2 cut(s) 321, 429
NlaIII CATG 5 cut(s) 226, 274, 370, 392, 404
NlaIV GGNNCC 2 cut(s) 121, 424
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 404
PctI GAATGC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 121, 424
PspPI GGNCC 2 cut(s) 119, 348
PsyI GACNNNGTC 1 cut(s) 102
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 2 cut(s) 321, 429
Sau96I GGNCC 2 cut(s) 119, 348
SduI GDGCHC 1 cut(s) 235
SetI ASST 2 cut(s) 171, 428
SfaNI GCATC 2 cut(s) 365, 477
Sse9I AATT 1 cut(s) 488
SsiI CCGC 1 cut(s) 433
StyI CCWWGG 2 cut(s) 351, 388
TaaI ACNGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 106
TasI AATT 1 cut(s) 488
TatI WGTACW 1 cut(s) 154
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 383
Tth111I GACNNNGTC 1 cut(s) 102
XceI RCATGY 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.