RLG00000036470

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001631
Physical Location & Seq
Reverse (-)
81025737 .. 81029481
3745 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036470

Sequence Viewer

Length: 603 bp
ATGATCTGGAGTTCGACGCCGCACCGGAGAGAGAAAGCGCTAATCCTAATTTCGACGCCGCACCGGAGGTGCGGATGCACTTCATTCTCTACGCTGACACATATGGCTTTCTTAGCAACTGCTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAGTGGAGACCTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAAAGTCGAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGATAACGTTTTAGTTAGAGAGCTTCTCGAAACCAGAGGCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGATGTGCTTTAGTTGGAGGGAACTTGGGACAGTTGGCTCAAAACATGGGATGGGCAGGGATTGTGATCAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTGAGAGCTTTGGCATCTCATCCCATGAAGTCCAACAAAAGAGGCCATGGCGATAAACATGTTCCGATCAACATTGCAGGAACCTTGATCCGCGAAGGGGAATGGTTGTATGCTGATAGTGATGGCATCCTTATCTCAACATCTGAATTGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

201

Amino Acids

21.56

Weight (kDa)

6.58

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 40 - 193 3.6e-46 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 174
AccII CGCG 1 cut(s) 537
AciI CCGC 4 cut(s) 20, 59, 72, 535
AclI AACGTT 1 cut(s) 255
AclWI GGATC 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 144
AcyI GRCGYC 2 cut(s) 17, 56
AfeI AGCGCT 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 541
AflIII ACRYGT 1 cut(s) 502
AluBI AGCT 3 cut(s) 146, 271, 452
AluI AGCT 3 cut(s) 146, 271, 452
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 1 cut(s) 526
Aor51HI AGCGCT 1 cut(s) 39
AoxI GGCC 2 cut(s) 220, 487
ApeKI GCWGC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 457
BbvI GCAGC 1 cut(s) 389
BccI CCATC 3 cut(s) 305, 372, 560
BclI TGATCA 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 174
BisI GCNGC 3 cut(s) 20, 59, 403
BlsI GCNGC 3 cut(s) 21, 60, 404
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 223, 526
BmsI GCATC 3 cut(s) 65, 467, 579
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 392
BsaHI GRCGYC 2 cut(s) 17, 56
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 490
BsaWI WCCGGW 2 cut(s) 24, 63
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 516
Bse8I GATNNNNATC 1 cut(s) 392
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 5 cut(s) 80, 383, 437, 463, 570
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 222, 489
BsiSI CCGG 2 cut(s) 25, 64
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 2 cut(s) 222, 489
Bso31I GGTCTC 1 cut(s) 156
Bsp143I GATC 5 cut(s) 3, 393, 423, 510, 531
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 4 cut(s) 20, 59, 72, 535
BspANI GGCC 2 cut(s) 222, 489
BspCNI CTCAG 1 cut(s) 230
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 223, 526
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 1 cut(s) 526
BspTNI GGTCTC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 516
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 5 cut(s) 3, 393, 423, 510, 531
BssNI GRCGYC 2 cut(s) 17, 56
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 235, 360
Bst6I CTCTTC 1 cut(s) 289
BstACI GRCGYC 2 cut(s) 17, 56
BstDEI CTNAG 2 cut(s) 112, 217
BstDSI CCRYGG 1 cut(s) 490
BstF5I GGATG 5 cut(s) 80, 383, 437, 463, 570
BstFNI CGCG 1 cut(s) 537
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 5 cut(s) 6, 396, 426, 513, 534
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 5 cut(s) 3, 393, 423, 510, 531
BstMWI GCNNNNNNNGC 2 cut(s) 113, 175
BstNSI RCATGY 1 cut(s) 506
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 1 cut(s) 389
BsuRI GGCC 2 cut(s) 222, 489
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 5 cut(s) 80, 383, 437, 463, 570
BtsIMutI CAGTG 1 cut(s) 231
BveI ACCTGC 1 cut(s) 174
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 2 cut(s) 25, 64
CviAII CATG 5 cut(s) 325, 373, 469, 491, 503
CviJI RGCY 8 cut(s) 107, 146, 222, 271, 365, 402, 452, 489
CviKI_1 RGCY 8 cut(s) 107, 146, 222, 271, 365, 402, 452, 489
DdeI CTNAG 2 cut(s) 112, 217
DpnI GATC 5 cut(s) 5, 395, 425, 512, 533
DpnII GATC 5 cut(s) 3, 393, 423, 510, 531
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 156
Eco47III AGCGCT 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 5 cut(s) 328, 376, 472, 494, 506
FaiI YATR 9 cut(s) 102, 104, 198, 326, 374, 470, 492, 504, 555
FaqI GGGAC 1 cut(s) 369
FatI CATG 5 cut(s) 324, 372, 468, 490, 502
FauNDI CATATG 1 cut(s) 102
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 20, 59, 403
FokI GGATG 5 cut(s) 87, 390, 444, 450, 557
Fsp4HI GCNGC 3 cut(s) 20, 59, 403
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 20, 59, 403
GsuI CTGGAG 1 cut(s) 28
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 2 cut(s) 222, 489
HapII CCGG 2 cut(s) 25, 64
HgaI GACGC 2 cut(s) 25, 64
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 17, 56
Hin1II CATG 5 cut(s) 328, 376, 472, 494, 506
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HpaII CCGG 2 cut(s) 25, 64
HphI GGTGA 1 cut(s) 457
Hpy188I TCNGA 3 cut(s) 192, 510, 589
Hpy188III TCNNGA 2 cut(s) 7, 275
Hpy99I CGWCG 2 cut(s) 19, 58
HpyAV CCTTC 1 cut(s) 533
HpyCH4III ACNGT 2 cut(s) 235, 360
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 3 cut(s) 78, 405, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 175
HpyF3I CTNAG 2 cut(s) 112, 217
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 2 cut(s) 17, 56
Hsp92II CATG 5 cut(s) 328, 376, 472, 494, 506
HspAI GCGC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 5 cut(s) 3, 393, 423, 510, 531
LpnPI CCDG 8 cut(s) 38, 77, 179, 188, 204, 295, 369, 507
Lsp1109I GCAGC 1 cut(s) 389
LweI GCATC 3 cut(s) 65, 467, 579
MaeII ACGT 1 cut(s) 255
MalI GATC 5 cut(s) 5, 395, 425, 512, 533
MboI GATC 5 cut(s) 3, 393, 423, 510, 531
MboII GAAGA 1 cut(s) 306
MluCI AATT 2 cut(s) 48, 590
MmeI TCCRAC 2 cut(s) 322, 501
MnlI CCTC 7 cut(s) 60, 241, 278, 283, 311, 338, 479
MseI TTAA 1 cut(s) 237
MspI CCGG 2 cut(s) 25, 64
Mva1269I GAATGC 1 cut(s) 212
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 2 cut(s) 113, 175
NcoI CCATGG 1 cut(s) 490
NdeI CATATG 1 cut(s) 102
NdeII GATC 5 cut(s) 3, 393, 423, 510, 531
NlaIII CATG 5 cut(s) 328, 376, 472, 494, 506
NlaIV GGNNCC 2 cut(s) 223, 526
NspI RCATGY 1 cut(s) 506
PciI ACATGT 1 cut(s) 502
PctI GAATGC 1 cut(s) 212
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 3 cut(s) 21, 60, 404
PscI ACATGT 1 cut(s) 502
Psp1406I AACGTT 1 cut(s) 255
PspN4I GGNNCC 2 cut(s) 223, 526
PspPI GGNCC 1 cut(s) 221
PsyI GACNNNGTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 3 cut(s) 20, 59, 403
Sau3AI GATC 5 cut(s) 3, 393, 423, 510, 531
Sau96I GGNCC 1 cut(s) 221
SetI ASST 8 cut(s) 71, 148, 168, 243, 258, 273, 454, 530
SfaNI GCATC 3 cut(s) 65, 467, 579
Sse9I AATT 2 cut(s) 48, 590
SsiI CCGC 4 cut(s) 20, 59, 72, 535
SspI AATATT 1 cut(s) 186
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 2 cut(s) 235, 360
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 14, 53, 208, 276
TasI AATT 2 cut(s) 48, 590
TauI GCSGC 2 cut(s) 22, 61
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 238
TseI GCWGC 1 cut(s) 402
TspDTI ATGAA 2 cut(s) 72, 485
TspRI CASTG 1 cut(s) 238
Tth111I GACNNNGTC 1 cut(s) 204
XceI RCATGY 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.