Rh6BG395400

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001631
Physical Location & Seq
Reverse (-)
62357733 .. 62359013
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG395400.1

Sequence Viewer

Length: 945 bp
ATGATCGATTCAGTTCCGGCTCCTGAAGTCGATCATGGATATTCATACACCACTCTCACCGTAGAAAAAATATGCAGCATCGTAAATTTCAAGCTCAAGACGCGCGAGATGTACTTTTTCTGGAAGACGCTTTTTGAAGATATCTTCAAGCGTTACGAGCTCACTGGCATTGTCAACGGCAGCGAACCCTGTCCCTATCGGTTCCTCATTGATAAGAACGGAAACCTCACGAATCAAGTCAATCTGGCCTTCGATGTTTGGCATGAGAAGGATCAGAGGATCATAACGTGGATCAAATCGACTCTTTCCGAAGACGTGGTGCTCCCACTAGTCACAACCGGAGTCATACCTTCCAATTCGTCTTGTGAGCTTTGGCTTAGCCTCGGGAAGAGATTCGCAGTCACGGGGGTGTCAGCCGAGTACAGGAGAAGTCTGGCCGCGGCCGCCTTGGCAACCACCGACATTTGTGACGCAAATCCCGCTCGGTTGGAGAGCGGGGACTTGCGTCTTTTGGAGCCAAATTTTCAAAGATTCGGAAAATGGCACGCATTTTCGGGTCGGGTGGTGACCTTGAAGGTGTTTGAGGACAACACGCTGGTGATTGAAGCTTTGTCGAGAAAAGGGGAGGGGAGGGTGTTGGTTGTGGACGGCGGAGGAAGCATGAGACGTGCCGTCACCGGAGGGATGCTGGGCAAGTGTGCCGAGATTATGGGGTGGGCTGGGATTGTGGTGAATGGGTGCGTAAGAGATGTGGATGAGATCAATGAATGTGAGATTGGGGTGAGAGCTTTGGCGACTTGTCCGGTGAGACCGATTAAAGGTGAAGATGGGAGTGGCCAGAAGCATGTTGATGTCAACATTGGAGGAACATGGATTCGTGACGGGGACTGGTTGTATGCGGATAATGATGGCATCCTTGTCTCCACGACTAAATTGTCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

314

Amino Acids

34.73

Weight (kDa)

5.55

Isoelectric Point (pI)

26.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 152 - 307 3.6e-43 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 934
AccBSI CCGCTC 2 cut(s) 482, 495
AccII CGCG 3 cut(s) 103, 105, 440
AciI CCGC 7 cut(s) 438, 440, 444, 480, 495, 651, 899
AclWI GGATC 3 cut(s) 279, 287, 299
AcoI YGGCCR 3 cut(s) 435, 441, 835
AcsI RAATTY 2 cut(s) 85, 520
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 2 cut(s) 113, 422
AfiI CCNNNNNNNGG 2 cut(s) 423, 818
AgsI TTSAA 6 cut(s) 91, 137, 148, 527, 574, 605
AhdI GACNNNNNGTC 1 cut(s) 671
AhlI ACTAGT 1 cut(s) 328
AjiI CACGTC 2 cut(s) 316, 668
AjuI GAANNNNNNNTTGG 2 cut(s) 759, 791
AleI CACNNNNGTG 2 cut(s) 407, 596
AluBI AGCT 5 cut(s) 94, 160, 370, 608, 788
AluI AGCT 5 cut(s) 94, 160, 370, 608, 788
Alw21I GWGCWC 2 cut(s) 162, 324
Alw26I GTCTC 3 cut(s) 658, 802, 925
AlwI GGATC 3 cut(s) 279, 287, 299
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 4 cut(s) 246, 435, 441, 835
ApeKI GCWGC 2 cut(s) 75, 180
ApoI RAATTY 2 cut(s) 85, 520
Asp700I GAANNNNTTC 1 cut(s) 392
AspLEI GCGC 1 cut(s) 105
AsuHPI GGTGA 8 cut(s) 49, 577, 610, 667, 742, 793, 817, 833
AvaI CYCGRG 1 cut(s) 383
BalI TGGCCA 1 cut(s) 837
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 2 cut(s) 131, 318
Bbv12I GWGCWC 2 cut(s) 162, 324
BbvI GCAGC 2 cut(s) 87, 192
BccI CCATC 2 cut(s) 821, 902
BceAI ACGGC 3 cut(s) 193, 656, 664
BcoDI GTCTC 3 cut(s) 658, 802, 925
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 1 cut(s) 329
BglI GCCNNNNNGGC 1 cut(s) 449
BisI GCNGC 5 cut(s) 76, 181, 438, 441, 444
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 5 cut(s) 77, 182, 439, 442, 445
BmeRI GACNNNNNGTC 1 cut(s) 671
BmeT110I CYCGRG 1 cut(s) 383
BmgBI CACGTC 2 cut(s) 316, 668
BmiI GGNNCC 3 cut(s) 21, 203, 516
BmsI GCATC 3 cut(s) 87, 675, 921
BoxI GACNNNNGTC 1 cut(s) 504
BpiI GAAGAC 2 cut(s) 131, 318
Bpu1102I GCTNAGC 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 802
BsaJI CCNNGG 3 cut(s) 382, 438, 447
BsaWI WCCGGW 3 cut(s) 338, 677, 802
Bsc4I CCNNNNNNNGG 2 cut(s) 423, 818
Bse1I ACTGG 2 cut(s) 169, 893
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 382, 438, 447
BseGI GGATG 3 cut(s) 690, 760, 912
BseLI CCNNNNNNNGG 2 cut(s) 423, 818
BseNI ACTGG 2 cut(s) 169, 893
BseX3I CGGCCG 1 cut(s) 441
BseXI GCAGC 2 cut(s) 87, 192
BseYI CCCAGC 2 cut(s) 688, 719
Bsh1236I CGCG 3 cut(s) 103, 105, 440
Bsh1285I CGRYCG 1 cut(s) 444
BshFI GGCC 4 cut(s) 248, 437, 443, 837
BshVI ATCGAT 1 cut(s) 6
BsiEI CGRYCG 1 cut(s) 444
BsiHKAI GWGCWC 2 cut(s) 162, 324
BsiHKCI CYCGRG 1 cut(s) 383
BsiSI CCGG 4 cut(s) 17, 339, 678, 803
BslFI GGGAC 3 cut(s) 177, 512, 899
BslI CCNNNNNNNGG 2 cut(s) 423, 818
BsmAI GTCTC 3 cut(s) 658, 802, 925
BsmBI CGTCTC 1 cut(s) 658
BsmFI GGGAC 3 cut(s) 177, 512, 899
BsnI GGCC 4 cut(s) 248, 437, 443, 837
Bso31I GGTCTC 1 cut(s) 802
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 162, 324
Bsp143I GATC 6 cut(s) 3, 31, 271, 279, 291, 759
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 7 cut(s) 438, 440, 444, 480, 495, 651, 899
BspANI GGCC 4 cut(s) 248, 437, 443, 837
BspDI ATCGAT 1 cut(s) 6
BspFNI CGCG 3 cut(s) 103, 105, 440
BspLI GGNNCC 3 cut(s) 21, 203, 516
BspPI GGATC 3 cut(s) 279, 287, 299
BspTNI GGTCTC 1 cut(s) 802
BsrBI CCGCTC 2 cut(s) 482, 495
BsrI ACTGG 2 cut(s) 169, 893
BssECI CCNNGG 3 cut(s) 382, 438, 447
BssMI GATC 6 cut(s) 3, 31, 271, 279, 291, 759
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 438
BstEII GGTNACC 1 cut(s) 565
BstF5I GGATG 3 cut(s) 690, 760, 912
BstFNI CGCG 3 cut(s) 103, 105, 440
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 6 cut(s) 6, 34, 274, 282, 294, 762
BstMAI GTCTC 3 cut(s) 658, 802, 925
BstMBI GATC 6 cut(s) 3, 31, 271, 279, 291, 759
BstMCI CGRYCG 1 cut(s) 444
BstMWI GCNNNNNNNGC 6 cut(s) 100, 157, 443, 449, 479, 657
BstNSI RCATGY 1 cut(s) 848
BstPAI GACNNNNGTC 1 cut(s) 504
BstPI GGTNACC 1 cut(s) 565
BstUI CGCG 3 cut(s) 103, 105, 440
BstV1I GCAGC 2 cut(s) 87, 192
BstV2I GAAGAC 2 cut(s) 131, 318
BstZI CGGCCG 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 4 cut(s) 248, 437, 443, 837
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 438
BtrI CACGTC 2 cut(s) 316, 668
BtsCI GGATG 3 cut(s) 690, 760, 912
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 546
CciNI GCGGCCGC 1 cut(s) 441
CfoI GCGC 1 cut(s) 105
Cfr42I CCGCGG 1 cut(s) 441
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 4 cut(s) 109, 136, 479, 494
Csp6I GTAC 2 cut(s) 112, 421
CviAII CATG 5 cut(s) 35, 263, 661, 845, 870
CviQI GTAC 2 cut(s) 112, 421
DdeI CTNAG 1 cut(s) 377
DpnI GATC 6 cut(s) 5, 33, 273, 281, 293, 761
DpnII GATC 6 cut(s) 3, 31, 271, 279, 291, 759
DrdI GACNNNNNNGTC 1 cut(s) 934
DriI GACNNNNNGTC 1 cut(s) 671
DseDI GACNNNNNNGTC 1 cut(s) 934
EaeI YGGCCR 3 cut(s) 435, 441, 835
EagI CGGCCG 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 383
Eam1105I GACNNNNNGTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 383
EciI GGCGGA 1 cut(s) 666
Ecl136II GAGCTC 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 802
Eco32I GATATC 1 cut(s) 142
Eco52I CGGCCG 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 565
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 658
FaeI CATG 5 cut(s) 38, 266, 664, 848, 873
FaqI GGGAC 3 cut(s) 177, 512, 899
FatI CATG 5 cut(s) 34, 262, 660, 844, 869
FauI CCCGC 2 cut(s) 487, 488
Fnu4HI GCNGC 5 cut(s) 76, 181, 438, 441, 444
FokI GGATG 3 cut(s) 697, 767, 899
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 5 cut(s) 76, 181, 438, 441, 444
FspBI CTAG 1 cut(s) 329
GlaI GCGC 1 cut(s) 104
GluI GCNGC 5 cut(s) 76, 181, 438, 441, 444
GsaI CCCAGC 2 cut(s) 692, 723
HaeIII GGCC 4 cut(s) 248, 437, 443, 837
HapII CCGG 4 cut(s) 17, 339, 678, 803
HgaI GACGC 4 cut(s) 109, 136, 479, 494
HhaI GCGC 1 cut(s) 105
Hin1II CATG 5 cut(s) 38, 266, 664, 848, 873
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HincII GTYRAC 2 cut(s) 175, 856
HindII GTYRAC 2 cut(s) 175, 856
HindIII AAGCTT 1 cut(s) 606
HinfI GANTC 7 cut(s) 8, 232, 301, 342, 393, 531, 874
HpaII CCGG 4 cut(s) 17, 339, 678, 803
HphI GGTGA 8 cut(s) 49, 577, 610, 667, 742, 793, 817, 833
Hpy166II GTNNAC 3 cut(s) 175, 646, 856
Hpy188I TCNGA 3 cut(s) 276, 310, 536
Hpy188III TCNNGA 7 cut(s) 23, 97, 121, 229, 385, 615, 878
Hpy8I GTNNAC 3 cut(s) 175, 646, 856
HpyAV CCTTC 4 cut(s) 259, 262, 360, 568
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4IV ACGT 3 cut(s) 287, 315, 667
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 157, 443, 449, 479, 657
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 3 cut(s) 287, 315, 667
Hsp92II CATG 5 cut(s) 38, 266, 664, 848, 873
HspAI GCGC 1 cut(s) 103
KspI CCGCGG 1 cut(s) 441
Kzo9I GATC 6 cut(s) 3, 31, 271, 279, 291, 759
LmnI GCTCC 3 cut(s) 25, 327, 514
Lsp1109I GCAGC 2 cut(s) 87, 192
LweI GCATC 3 cut(s) 87, 675, 921
MaeI CTAG 1 cut(s) 329
MaeII ACGT 3 cut(s) 287, 315, 667
MaeIII GTNAC 7 cut(s) 152, 331, 400, 467, 565, 673, 878
MalI GATC 6 cut(s) 5, 33, 273, 281, 293, 761
MbiI CCGCTC 2 cut(s) 482, 495
MboI GATC 6 cut(s) 3, 31, 271, 279, 291, 759
MboII GAAGA 6 cut(s) 136, 136, 149, 323, 400, 836
MhlI GDGCHC 2 cut(s) 162, 324
MlsI TGGCCA 1 cut(s) 837
MluCI AATT 5 cut(s) 85, 355, 520, 932, 939
MluNI TGGCCA 1 cut(s) 837
MlyI GAGTC 2 cut(s) 295, 351
MmeI TCCRAC 1 cut(s) 468
Mox20I TGGCCA 1 cut(s) 837
MroXI GAANNNNTTC 1 cut(s) 392
MscI TGGCCA 1 cut(s) 837
MseI TTAA 1 cut(s) 816
MslI CAYNNNNRTG 3 cut(s) 407, 596, 849
Msp20I TGGCCA 1 cut(s) 837
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 4 cut(s) 17, 339, 678, 803
MvnI CGCG 3 cut(s) 103, 105, 440
MwoI GCNNNNNNNGC 6 cut(s) 100, 157, 443, 449, 479, 657
NdeII GATC 6 cut(s) 3, 31, 271, 279, 291, 759
NlaIII CATG 5 cut(s) 38, 266, 664, 848, 873
NlaIV GGNNCC 3 cut(s) 21, 203, 516
NmeAIII GCCGAG 2 cut(s) 442, 727
NmuCI GTSAC 6 cut(s) 331, 400, 467, 565, 673, 878
NotI GCGGCCGC 1 cut(s) 441
NspI RCATGY 1 cut(s) 848
OliI CACNNNNGTG 2 cut(s) 407, 596
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 5 cut(s) 8, 232, 393, 531, 874
PkrI GCNGC 5 cut(s) 77, 182, 439, 442, 445
PleI GAGTC 2 cut(s) 295, 350
PpsI GAGTC 2 cut(s) 295, 350
PshAI GACNNNNGTC 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 162
PspEI GGTNACC 1 cut(s) 565
PspFI CCCAGC 2 cut(s) 688, 719
PspN4I GGNNCC 3 cut(s) 21, 203, 516
RsaI GTAC 2 cut(s) 113, 422
RsaNI GTAC 2 cut(s) 112, 421
RseI CAYNNNNRTG 3 cut(s) 407, 596, 849
SacI GAGCTC 1 cut(s) 162
SacII CCGCGG 1 cut(s) 441
SaqAI TTAA 1 cut(s) 816
SatI GCNGC 5 cut(s) 76, 181, 438, 441, 444
Sau3AI GATC 6 cut(s) 3, 31, 271, 279, 291, 759
SchI GAGTC 2 cut(s) 295, 351
SduI GDGCHC 2 cut(s) 162, 324
SfaNI GCATC 3 cut(s) 87, 675, 921
Sfr303I CCGCGG 1 cut(s) 441
SgrBI CCGCGG 1 cut(s) 441
SmiMI CAYNNNNRTG 3 cut(s) 407, 596, 849
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SpeI ACTAGT 1 cut(s) 328
Sse9I AATT 5 cut(s) 85, 355, 520, 932, 939
SsiI CCGC 7 cut(s) 438, 440, 444, 480, 495, 651, 899
SspMI CTAG 1 cut(s) 329
SstI GAGCTC 1 cut(s) 162
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 1 cut(s) 61
TaiI ACGT 3 cut(s) 290, 318, 670
TaqI TCGA 5 cut(s) 6, 30, 252, 299, 614
TaqII GACCGA 1 cut(s) 826
TasI AATT 5 cut(s) 85, 355, 520, 932, 939
TatI WGTACW 2 cut(s) 111, 420
TauI GCSGC 3 cut(s) 440, 443, 446
TfiI GAWTC 5 cut(s) 8, 232, 393, 531, 874
Tru1I TTAA 1 cut(s) 816
Tru9I TTAA 1 cut(s) 816
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 6 cut(s) 331, 400, 467, 565, 673, 878
TseI GCWGC 2 cut(s) 75, 180
Tsp45I GTSAC 6 cut(s) 331, 400, 467, 565, 673, 878
TspDTI ATGAA 2 cut(s) 33, 780
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 169
XapI RAATTY 2 cut(s) 85, 520
XceI RCATGY 1 cut(s) 848
XmnI GAANNNNTTC 1 cut(s) 392
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.