pycom03g03450

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001631
Physical Location & Seq
Forward (+)
2745856 .. 2746356
501 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03450.1

Sequence Viewer

Length: 501 bp
ATGGCTACGTTGGCAACTGCTGAAATTTGTGATTCGAATACCACTCTTTTGGAAAGTGGATGTTTGCGTGTGCTGCCACCAATTTTCCAGACATACGGACAATCTCGAGCATTCTCAGGCCCCATTACCACACTTAAGGTGTTTGAGGACAGTGTCTTGGTCAGACAGCTTCTCGAGACCAAAGGCGAGGGAAGAGTTCTGGTTATTGACGGCGGAGGAAGCATGAGATGTTCTTTGGTTGGAGGGAACTTGGGACAGTTGGCTCAAAACATGGGATGGACAGGTATTGTGGTGAATGGCTGCATTAGAGATGTTGACGAGATCAATGAGTGTGACATTGGGGTGAGGGCTTTGGCATCTCATCCGGTGAAATCGAACAAAAAAGGCGTCGGAGAAAAGCATGTTCTGATCAACATTGCGGGAACCTTGATTCGTGAAGGGGAATGGTTGTATGCAGATAATGATGGCATCCTTATTTCAGCATCTGAATTGTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.66

Weight (kDa)

5.01

Isoelectric Point (pI)

31.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 8.1e-44 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 213, 419
AcsI RAATTY 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 387
AflII CTTAAG 1 cut(s) 134
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 485
Ama87I CYCGRG 2 cut(s) 105, 173
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 2 cut(s) 73, 300
ApoI RAATTY 1 cut(s) 24
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 304, 355, 379
AsuII TTCGAA 1 cut(s) 35
AvaI CYCGRG 2 cut(s) 105, 173
BbvI GCAGC 2 cut(s) 60, 287
BccI CCATC 2 cut(s) 270, 458
BceAI ACGGC 1 cut(s) 226
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 1 cut(s) 170
BfrI CTTAAG 1 cut(s) 134
BisI GCNGC 2 cut(s) 74, 301
BlsI GCNGC 2 cut(s) 75, 302
BmeT110I CYCGRG 2 cut(s) 105, 173
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 121, 424
BmsI GCATC 3 cut(s) 365, 477, 491
Bpu14I TTCGAA 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 170
BsaWI WCCGGW 1 cut(s) 364
Bse3DI GCAATG 1 cut(s) 414
BseGI GGATG 4 cut(s) 65, 281, 361, 468
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 60, 287
BshFI GGCC 1 cut(s) 120
BsiHKCI CYCGRG 2 cut(s) 105, 173
BsiSI CCGG 1 cut(s) 365
BslFI GGGAC 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 267
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 2 cut(s) 105, 173
Bsp119I TTCGAA 1 cut(s) 35
Bsp143I GATC 2 cut(s) 321, 408
BspACI CCGC 2 cut(s) 213, 419
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 121, 424
BspT104I TTCGAA 1 cut(s) 35
BspTI CTTAAG 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 414
BssMI GATC 2 cut(s) 321, 408
BssNI GRCGYC 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 152, 258
Bst6I CTCTTC 1 cut(s) 187
BstACI GRCGYC 1 cut(s) 387
BstAFI CTTAAG 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 35
BstDEI CTNAG 1 cut(s) 115
BstF5I GGATG 4 cut(s) 65, 281, 361, 468
BstKTI GATC 2 cut(s) 324, 411
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 2 cut(s) 321, 408
BstMWI GCNNNNNNNGC 3 cut(s) 11, 73, 219
BstNSI RCATGY 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 60, 287
BstXI CCANNNNNNTGG 1 cut(s) 49
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 4 cut(s) 65, 281, 361, 468
BtsIMutI CAGTG 1 cut(s) 157
CaiI CAGNNNCTG 1 cut(s) 485
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 1 cut(s) 376
CviAII CATG 3 cut(s) 223, 271, 401
CviJI RGCY 6 cut(s) 5, 120, 169, 263, 300, 350
CviKI_1 RGCY 6 cut(s) 5, 120, 169, 263, 300, 350
DdeI CTNAG 1 cut(s) 115
DpnI GATC 2 cut(s) 323, 410
DpnII GATC 2 cut(s) 321, 408
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
EciI GGCGGA 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 170
Eco88I CYCGRG 2 cut(s) 105, 173
EcoO109I RGGNCCY 1 cut(s) 119
FaeI CATG 3 cut(s) 226, 274, 404
FaiI YATR 5 cut(s) 94, 224, 272, 402, 453
FaqI GGGAC 1 cut(s) 267
FatI CATG 3 cut(s) 222, 270, 400
FauI CCCGC 1 cut(s) 412
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 74, 301
FokI GGATG 4 cut(s) 72, 288, 348, 455
Fsp4HI GCNGC 2 cut(s) 74, 301
GluI GCNGC 2 cut(s) 74, 301
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 365
HgaI GACGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 3 cut(s) 226, 274, 404
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 2 cut(s) 32, 430
HpaII CCGG 1 cut(s) 365
HphI GGTGA 3 cut(s) 304, 355, 379
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 164, 392, 408, 487
Hpy188III TCNNGA 5 cut(s) 88, 105, 173, 175, 434
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 2 cut(s) 152, 258
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 303, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 73, 219
HpyF3I CTNAG 1 cut(s) 115
HpySE526I ACGT 1 cut(s) 8
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 3 cut(s) 226, 274, 404
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 2 cut(s) 321, 408
LpnPI CCDG 5 cut(s) 101, 102, 185, 267, 378
Lsp1109I GCAGC 2 cut(s) 60, 287
LweI GCATC 3 cut(s) 365, 477, 491
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 332
MalI GATC 2 cut(s) 323, 410
MboI GATC 2 cut(s) 321, 408
MboII GAAGA 1 cut(s) 204
MluCI AATT 3 cut(s) 24, 81, 488
MmeI TCCRAC 2 cut(s) 220, 370
MnlI CCTC 5 cut(s) 139, 181, 209, 236, 339
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 1 cut(s) 341
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 11, 73, 219
NdeII GATC 2 cut(s) 321, 408
NlaIII CATG 3 cut(s) 226, 274, 404
NlaIV GGNNCC 2 cut(s) 121, 424
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 404
NspV TTCGAA 1 cut(s) 35
PaeR7I CTCGAG 2 cut(s) 105, 173
PctI GAATGC 1 cut(s) 110
PfeI GAWTC 2 cut(s) 32, 430
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 75, 302
PspN4I GGNNCC 2 cut(s) 121, 424
PspPI GGNCC 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 485
PsyI GACNNNGTC 1 cut(s) 152
RseI CAYNNNNRTG 1 cut(s) 341
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 2 cut(s) 74, 301
Sau3AI GATC 2 cut(s) 321, 408
Sau96I GGNCC 1 cut(s) 119
SetI ASST 5 cut(s) 11, 141, 171, 286, 428
SfaNI GCATC 3 cut(s) 365, 477, 491
Sfr274I CTCGAG 2 cut(s) 105, 173
SfuI TTCGAA 1 cut(s) 35
SlaI CTCGAG 2 cut(s) 105, 173
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 3 cut(s) 105, 134, 173
SmoI CTYRAG 3 cut(s) 105, 134, 173
Sse9I AATT 3 cut(s) 24, 81, 488
SsiI CCGC 2 cut(s) 213, 419
TaaI ACNGT 2 cut(s) 152, 258
TaiI ACGT 1 cut(s) 11
TaqI TCGA 4 cut(s) 35, 106, 174, 374
TasI AATT 3 cut(s) 24, 81, 488
TfiI GAWTC 2 cut(s) 32, 430
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 73, 300
Tsp45I GTSAC 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 157
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 134
XapI RAATTY 1 cut(s) 24
XceI RCATGY 1 cut(s) 404
XhoI CTCGAG 2 cut(s) 105, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.