pycom11g03860

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001631
Physical Location & Seq
Forward (+)
3020333 .. 3020851
519 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03860.1

Sequence Viewer

Length: 519 bp
ATGATATGCAGGCGAAATATGGCCACCTTAGCAACTGCTGAAATTTGTGATACGAATACAACTCTTGTGGGAAGCGGAGGTTTGCGTGTGCTGCCACCAATTTTCCAGGTATACGGACAATCCCGAGCATTCTCAGGCCCCATTACGACACTCAAGGTGTTCGAGGACAATGTCTTGGTCAGACAGCTTCTCGAAACCAAAGGCGAGGGAAGAGTTTTGGTCATTGACGGAGGAGGAAGCATGAGATGTGCTTTGGTTGGAGGGAACTTGGGACAGTTGGCCCAAAACATGGGATGGGCAGGTATTGTGGTGAATGGCTGCATTAGAGATGTAGATGAGATCAATGAGTGTGATATTGGGGTGAGGGCTTTGGCATCTCATCCGATGAAATCGAACAAAAAAGGTGTCGGAGAAAAGCATGTTCCGATCAACATTGCTGGAACCTTGATTCGTGAAGGCGAATGGTTGTATGCAGATAACGATGGCGTCCTTATTTCGACATCTGAATTGTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

173

Amino Acids

18.33

Weight (kDa)

5.69

Isoelectric Point (pI)

27.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 12 - 165 3.2e-47 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 290
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 111
AciI CCGC 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 486
AfiI CCNNNNNNNGG 1 cut(s) 289
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 1 cut(s) 187
AluI AGCT 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 123
AoxI GGCC 3 cut(s) 21, 136, 279
ApeKI GCWGC 2 cut(s) 91, 318
ApoI RAATTY 1 cut(s) 42
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
AspS9I GGNCC 2 cut(s) 137, 280
AsuHPI GGTGA 2 cut(s) 322, 373
AvaI CYCGRG 1 cut(s) 123
BalI TGGCCA 1 cut(s) 23
BbvI GCAGC 2 cut(s) 78, 305
BccI CCATC 2 cut(s) 288, 476
BciT130I CCWGG 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 290
BisI GCNGC 2 cut(s) 92, 319
BlsI GCNGC 2 cut(s) 93, 320
Bme1390I CCNGG 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 123
BmgT120I GGNCC 2 cut(s) 137, 280
BmiI GGNNCC 2 cut(s) 139, 442
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 383
Bpu10I CCTNAGC 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 486
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 107
BseGI GGATG 2 cut(s) 299, 379
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 2 cut(s) 78, 305
BshFI GGCC 3 cut(s) 23, 138, 281
BsiHKCI CYCGRG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 3 cut(s) 23, 138, 281
BsoBI CYCGRG 1 cut(s) 123
Bsp143I GATC 2 cut(s) 339, 426
BspACI CCGC 1 cut(s) 75
BspANI GGCC 3 cut(s) 23, 138, 281
BspCNI CTCAG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 139, 442
BspMI ACCTGC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 432
BssMI GATC 2 cut(s) 339, 426
BssNAI GTATAC 1 cut(s) 112
BssNI GRCGYC 1 cut(s) 486
Bst1107I GTATAC 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 205
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 11
BstDEI CTNAG 2 cut(s) 28, 133
BstF5I GGATG 2 cut(s) 299, 379
BstKTI GATC 2 cut(s) 342, 429
BstMBI GATC 2 cut(s) 339, 426
BstMWI GCNNNNNNNGC 2 cut(s) 29, 91
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 78, 305
BstZ17I GTATAC 1 cut(s) 112
BsuRI GGCC 3 cut(s) 23, 138, 281
BtsCI GGATG 2 cut(s) 299, 379
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 137, 280
CseI GACGC 1 cut(s) 475
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 3 cut(s) 241, 289, 419
CviJI RGCY 6 cut(s) 23, 138, 187, 281, 318, 368
CviKI_1 RGCY 6 cut(s) 23, 138, 187, 281, 318, 368
DdeI CTNAG 2 cut(s) 28, 133
DpnI GATC 2 cut(s) 341, 428
DpnII GATC 2 cut(s) 339, 426
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 105
FaeI CATG 3 cut(s) 244, 292, 422
FaiI YATR 7 cut(s) 7, 20, 112, 242, 290, 420, 471
FaqI GGGAC 1 cut(s) 285
FatI CATG 3 cut(s) 240, 288, 418
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 2 cut(s) 92, 319
FokI GGATG 2 cut(s) 306, 366
Fsp4HI GCNGC 2 cut(s) 92, 319
GluI GCNGC 2 cut(s) 92, 319
HaeIII GGCC 3 cut(s) 23, 138, 281
HgaI GACGC 1 cut(s) 475
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 3 cut(s) 244, 292, 422
HinfI GANTC 1 cut(s) 448
HphI GGTGA 2 cut(s) 322, 373
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 5 cut(s) 182, 384, 410, 426, 505
Hpy188III TCNNGA 3 cut(s) 123, 191, 452
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 9, 321, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 91
HpyF3I CTNAG 2 cut(s) 28, 133
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 3 cut(s) 244, 292, 422
Kzo9I GATC 2 cut(s) 339, 426
LpnPI CCDG 5 cut(s) 92, 119, 120, 285, 423
Lsp1109I GCAGC 2 cut(s) 78, 305
LweI GCATC 1 cut(s) 383
MalI GATC 2 cut(s) 341, 428
MboI GATC 2 cut(s) 339, 426
MboII GAAGA 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 3 cut(s) 42, 99, 506
MluNI TGGCCA 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 238, 388
MnlI CCTC 7 cut(s) 71, 157, 199, 224, 227, 254, 357
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 29, 91
NdeII GATC 2 cut(s) 339, 426
NlaIII CATG 3 cut(s) 244, 292, 422
NlaIV GGNNCC 2 cut(s) 139, 442
NspI RCATGY 1 cut(s) 422
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 448
PflFI GACNNNGTC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 2 cut(s) 93, 320
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 139, 442
PspPI GGNCC 2 cut(s) 137, 280
PsyI GACNNNGTC 1 cut(s) 170
SatI GCNGC 2 cut(s) 92, 319
Sau3AI GATC 2 cut(s) 339, 426
Sau96I GGNCC 2 cut(s) 137, 280
ScrFI CCNGG 1 cut(s) 107
SetI ASST 8 cut(s) 29, 82, 111, 159, 189, 304, 406, 446
SfaNI GCATC 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 3 cut(s) 42, 99, 506
SsiI CCGC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 4 cut(s) 162, 192, 392, 497
TasI AATT 3 cut(s) 42, 99, 506
TfiI GAWTC 1 cut(s) 448
TseI GCWGC 2 cut(s) 91, 318
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 2 cut(s) 129, 243
Tth111I GACNNNGTC 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 289
XapI RAATTY 1 cut(s) 42
XceI RCATGY 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.