Rroxscaffold_1G00007180

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001631
Physical Location & Seq
Forward (+)
9211748 .. 9212206
459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007180.1

Sequence Viewer

Length: 459 bp
ATGGCTTTCTTAGCAACTGGTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAGTGGAGATTTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAACGTCGAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGACAACGTTTTGGTTAGAGAGCTTGTCGGAACCAGAGTCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGAAGTGCTTTGCTTGGAGGGAATTTGGCACGGTTGGCTCAAAACATGGGATGGGTAGGGATTGTGATAAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTGAGAGCTTTAGCATCTCATCCCGTGAAGCCCGGCAAAAGAGGCCATGGCAATAAGCACGTTCCGATCAACATTGCAGGAACCTTGATCCGCGAAAGGGGAATGGTTGTATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

152

Amino Acids

16.15

Weight (kDa)

9.16

Isoelectric Point (pI)

23.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 8 - 152 6.2e-37 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 435
AciI CCGC 1 cut(s) 433
AclI AACGTT 1 cut(s) 153
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 1 cut(s) 247
AfiI CCNNNNNNNGG 1 cut(s) 439
AluBI AGCT 3 cut(s) 44, 169, 350
AluI AGCT 3 cut(s) 44, 169, 350
AlwI GGATC 1 cut(s) 424
AoxI GGCC 2 cut(s) 118, 385
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 2 cut(s) 32, 355
BbvI GCAGC 1 cut(s) 287
BccI CCATC 2 cut(s) 203, 270
BcnI CCSGG 1 cut(s) 375
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 3 cut(s) 121, 178, 424
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 388
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 3 cut(s) 281, 335, 361
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 2 cut(s) 120, 387
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 439
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 2 cut(s) 120, 387
Bsp143I GATC 3 cut(s) 321, 408, 429
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 1 cut(s) 433
BspANI GGCC 2 cut(s) 120, 387
BspCNI CTCAG 1 cut(s) 128
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 121, 178, 424
BspPI GGATC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 3 cut(s) 321, 408, 429
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 133, 258
Bst6I CTCTTC 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 10, 115
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 3 cut(s) 281, 335, 361
BstFNI CGCG 1 cut(s) 435
BstKTI GATC 3 cut(s) 324, 411, 432
BstMBI GATC 3 cut(s) 321, 408, 429
BstMWI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
BstSCI CCNGG 1 cut(s) 373
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 120, 387
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 3 cut(s) 281, 335, 361
BtsIMutI CAGTG 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 119
CviAII CATG 3 cut(s) 223, 271, 389
CviJI RGCY 9 cut(s) 5, 44, 120, 169, 263, 300, 350, 373, 387
CviKI_1 RGCY 9 cut(s) 5, 44, 120, 169, 263, 300, 350, 373, 387
DdeI CTNAG 2 cut(s) 10, 115
DpnI GATC 3 cut(s) 323, 410, 431
DpnII GATC 3 cut(s) 321, 408, 429
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 388
EcoO109I RGGNCCY 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 3 cut(s) 226, 274, 392
FaiI YATR 5 cut(s) 96, 224, 272, 390, 454
FatI CATG 3 cut(s) 222, 270, 388
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 3 cut(s) 288, 342, 348
Fsp4HI GCNGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 120, 387
HapII CCGG 1 cut(s) 375
Hin1II CATG 3 cut(s) 226, 274, 392
HinfI GANTC 1 cut(s) 183
HpaII CCGG 1 cut(s) 375
HphI GGTGA 2 cut(s) 32, 355
Hpy188I TCNGA 3 cut(s) 90, 176, 408
Hpy99I CGWCG 1 cut(s) 108
HpyCH4III ACNGT 2 cut(s) 133, 258
HpyCH4IV ACGT 3 cut(s) 103, 153, 402
HpyCH4V TGCA 2 cut(s) 303, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
HpyF3I CTNAG 2 cut(s) 10, 115
HpySE526I ACGT 3 cut(s) 103, 153, 402
Hsp92II CATG 3 cut(s) 226, 274, 392
Kzo9I GATC 3 cut(s) 321, 408, 429
LpnPI CCDG 6 cut(s) 3, 86, 102, 193, 388, 405
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 365
MaeII ACGT 3 cut(s) 103, 153, 402
MalI GATC 3 cut(s) 323, 410, 431
MboI GATC 3 cut(s) 321, 408, 429
MboII GAAGA 1 cut(s) 204
MluCI AATT 1 cut(s) 247
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 5 cut(s) 139, 181, 209, 236, 377
MseI TTAA 1 cut(s) 135
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 110
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 388
NdeII GATC 3 cut(s) 321, 408, 429
NlaIII CATG 3 cut(s) 226, 274, 392
NlaIV GGNNCC 3 cut(s) 121, 178, 424
PctI GAATGC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 153
PspN4I GGNNCC 3 cut(s) 121, 178, 424
PspPI GGNCC 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 3 cut(s) 321, 408, 429
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 1 cut(s) 375
SetI ASST 8 cut(s) 46, 106, 141, 156, 171, 352, 405, 428
SfaNI GCATC 1 cut(s) 365
Sse9I AATT 1 cut(s) 247
SsiI CCGC 1 cut(s) 433
SspI AATATT 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 133, 258
TaiI ACGT 3 cut(s) 106, 156, 405
TaqI TCGA 2 cut(s) 106, 186
TasI AATT 1 cut(s) 247
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 300
TspRI CASTG 1 cut(s) 136
Tth111I GACNNNGTC 1 cut(s) 102
XapI RAATTY 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.