MD03G1044600.v1.1

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001631
Physical Location & Seq
Forward (+)
3545558 .. 3548144
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1044600.v1.1.491

Sequence Viewer

Length: 513 bp
ATGCTGCAAAATATGGCTACCTTGGCAACTGCTGAAATTTGTGATTCGAATACAACTCTTTTGGAAAGTGGAGATTTGCGTGTGCTGCCACCAATTTTCCAGACATACGGACAATCTCGAGTATTCTCAGGCCCCATTACCACACTAAAGGTATTTGAGGACAATGTCTTAGTCAGACAGCTTCTCGAGACCAAAGGCGAGGGAAGAGTTTTGGTTATTGACGGCGCAGGAAGCATGAGATGTGCTTTGGTTGGAGGGAACTTGGGACAGTTGGCTCAAAACATGGGATGGGCAGGTATTGTGGTGAATGGCTGCATTAGAGATGTAGACGAGATCAATGAGTGTGATATTGGGGTGAGGGCTTTGGCATCTCATCCGGTGAAATCGAACAAAAAAGGCGCCGGAGAAAAGCATGTTCTGATCAACATTGCGGGAACCTTGATTCGTGAAGGGGAATGGTTGTATGCAGATAATGATGGCATCCTTATTTCAGTATCTGAATTGTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

171

Amino Acids

18.18

Weight (kDa)

4.88

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 10 - 163 3e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 327
AciI CCGC 1 cut(s) 431
AcsI RAATTY 1 cut(s) 36
AcyI GRCGYC 1 cut(s) 399
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 182
AlwNI CAGNNNCTG 1 cut(s) 497
Ama87I CYCGRG 2 cut(s) 117, 185
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 3 cut(s) 4, 85, 312
ApoI RAATTY 1 cut(s) 36
AspLEI GCGC 2 cut(s) 227, 401
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 3 cut(s) 316, 367, 391
AsuII TTCGAA 1 cut(s) 47
AvaI CYCGRG 2 cut(s) 117, 185
BanI GGYRCC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 72, 299
BccI CCATC 2 cut(s) 282, 470
BceAI ACGGC 1 cut(s) 238
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 182
BfoI RGCGCY 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 3 cut(s) 5, 86, 313
BlsI GCNGC 3 cut(s) 6, 87, 314
BmeT110I CYCGRG 2 cut(s) 117, 185
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 3 cut(s) 133, 400, 436
BmsI GCATC 2 cut(s) 377, 489
Bpu14I TTCGAA 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 376
Bse3DI GCAATG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 3 cut(s) 293, 373, 480
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 141
BseXI GCAGC 2 cut(s) 72, 299
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 1 cut(s) 398
BsiHKCI CYCGRG 2 cut(s) 117, 185
BsiSI CCGG 2 cut(s) 377, 402
BslFI GGGAC 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 2 cut(s) 117, 185
Bsp119I TTCGAA 1 cut(s) 47
Bsp143I GATC 2 cut(s) 333, 420
BspACI CCGC 1 cut(s) 431
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 140
BspLI GGNNCC 3 cut(s) 133, 400, 436
BspMI ACCTGC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 426
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 333, 420
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 199
BstACI GRCGYC 1 cut(s) 399
BstBI TTCGAA 1 cut(s) 47
BstDEI CTNAG 2 cut(s) 127, 169
BstF5I GGATG 3 cut(s) 293, 373, 480
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 2 cut(s) 227, 401
BstKTI GATC 2 cut(s) 336, 423
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 333, 420
BstMWI GCNNNNNNNGC 3 cut(s) 23, 85, 231
BstNSI RCATGY 1 cut(s) 416
BstV1I GCAGC 2 cut(s) 72, 299
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 3 cut(s) 293, 373, 480
BveI ACCTGC 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 2 cut(s) 227, 401
Cfr13I GGNCC 1 cut(s) 131
CviAII CATG 3 cut(s) 235, 283, 413
CviJI RGCY 6 cut(s) 17, 132, 181, 275, 312, 362
CviKI_1 RGCY 6 cut(s) 17, 132, 181, 275, 312, 362
DdeI CTNAG 2 cut(s) 127, 169
DinI GGCGCC 1 cut(s) 400
DpnI GATC 2 cut(s) 335, 422
DpnII GATC 2 cut(s) 333, 420
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 182
Eco88I CYCGRG 2 cut(s) 117, 185
EcoO109I RGGNCCY 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 3 cut(s) 238, 286, 416
FaiI YATR 6 cut(s) 14, 106, 236, 284, 414, 465
FaqI GGGAC 1 cut(s) 279
FatI CATG 3 cut(s) 234, 282, 412
FauI CCCGC 1 cut(s) 424
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 3 cut(s) 5, 86, 313
FokI GGATG 3 cut(s) 300, 360, 467
Fsp4HI GCNGC 3 cut(s) 5, 86, 313
GlaI GCGC 2 cut(s) 226, 400
GluI GCNGC 3 cut(s) 5, 86, 313
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 377, 402
HhaI GCGC 2 cut(s) 227, 401
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 3 cut(s) 238, 286, 416
Hin6I GCGC 2 cut(s) 225, 399
HinP1I GCGC 2 cut(s) 225, 399
HinfI GANTC 2 cut(s) 44, 442
HpaII CCGG 2 cut(s) 377, 402
HphI GGTGA 3 cut(s) 316, 367, 391
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 3 cut(s) 176, 420, 499
Hpy188III TCNNGA 5 cut(s) 100, 117, 185, 187, 446
Hpy8I GTNNAC 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 7, 315, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 85, 231
HpyF3I CTNAG 2 cut(s) 127, 169
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 3 cut(s) 238, 286, 416
HspAI GCGC 2 cut(s) 225, 399
KasI GGCGCC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 420
Kzo9I GATC 2 cut(s) 333, 420
LpnPI CCDG 6 cut(s) 113, 114, 213, 279, 390, 415
Lsp1109I GCAGC 2 cut(s) 72, 299
LweI GCATC 2 cut(s) 377, 489
MalI GATC 2 cut(s) 335, 422
MboI GATC 2 cut(s) 333, 420
MboII GAAGA 1 cut(s) 216
MluCI AATT 3 cut(s) 36, 93, 500
Mly113I GGCGCC 1 cut(s) 399
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 4 cut(s) 151, 193, 248, 351
MspI CCGG 2 cut(s) 377, 402
MwoI GCNNNNNNNGC 3 cut(s) 23, 85, 231
NarI GGCGCC 1 cut(s) 399
NdeII GATC 2 cut(s) 333, 420
NlaIII CATG 3 cut(s) 238, 286, 416
NlaIV GGNNCC 3 cut(s) 133, 400, 436
NspI RCATGY 1 cut(s) 416
NspV TTCGAA 1 cut(s) 47
PaeR7I CTCGAG 2 cut(s) 117, 185
PfeI GAWTC 2 cut(s) 44, 442
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 3 cut(s) 6, 87, 314
PluTI GGCGCC 1 cut(s) 402
PspN4I GGNNCC 3 cut(s) 133, 400, 436
PspPI GGNCC 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 497
PsyI GACNNNGTC 1 cut(s) 164
SatI GCNGC 3 cut(s) 5, 86, 313
Sau3AI GATC 2 cut(s) 333, 420
Sau96I GGNCC 1 cut(s) 131
SetI ASST 5 cut(s) 23, 153, 183, 298, 440
SfaNI GCATC 2 cut(s) 377, 489
SfoI GGCGCC 1 cut(s) 400
Sfr274I CTCGAG 2 cut(s) 117, 185
SfuI TTCGAA 1 cut(s) 47
SlaI CTCGAG 2 cut(s) 117, 185
SmlI CTYRAG 2 cut(s) 117, 185
SmoI CTYRAG 2 cut(s) 117, 185
Sse9I AATT 3 cut(s) 36, 93, 500
SsiI CCGC 1 cut(s) 431
SspDI GGCGCC 1 cut(s) 398
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 270
TaqI TCGA 4 cut(s) 47, 118, 186, 386
TasI AATT 3 cut(s) 36, 93, 500
TfiI GAWTC 2 cut(s) 44, 442
TseI GCWGC 3 cut(s) 4, 85, 312
TspGWI ACGGA 1 cut(s) 123
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 1 cut(s) 36
XceI RCATGY 1 cut(s) 416
XhoI CTCGAG 2 cut(s) 117, 185
XmiI GTMKAC 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.