MD03G1045200.v1.1

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001631
Physical Location & Seq
Forward (+)
3613177 .. 3613431
255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1045200.v1.1.491

Sequence Viewer

Length: 255 bp
ATGTTGGCGAAGTGTGCCGAAATTATGGGATGGTCTGGGATTGTGATAAATGGGTGCATCAGAGATGTTGATGAGGTAAATGGATGTGAGATTGGTGTGAGAGCTTTGGCAACTTGTCCTGTGAGACCAGTCAAAAGTGATGGTGGCCAAAAGCATGTTCCTATCAACATTGGAGGAGTCTGGATTCATGAAGGAGAATGGTTGTATGCAGATGGTGATGGCATCCTCATCTCCACATCTCAATTGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

85

Amino Acids

8.93

Weight (kDa)

4.85

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 1 - 77 3.5e-24 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 118
AoxI GGCC 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 227
BalI TGGCCA 1 cut(s) 147
BccI CCATC 4 cut(s) 24, 134, 206, 212
BcoDI GTCTC 1 cut(s) 118
BmsI GCATC 2 cut(s) 66, 231
BsaI GGTCTC 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 128
BseGI GGATG 3 cut(s) 35, 89, 222
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 189
BshFI GGCC 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 118
BspANI GGCC 1 cut(s) 147
BspHI TCATGA 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 118
BsrI ACTGG 1 cut(s) 128
BstF5I GGATG 3 cut(s) 35, 89, 222
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 158
BsuRI GGCC 1 cut(s) 147
BtsCI GGATG 3 cut(s) 35, 89, 222
CciI TCATGA 1 cut(s) 187
CviAII CATG 2 cut(s) 155, 188
CviJI RGCY 2 cut(s) 104, 147
CviKI_1 RGCY 2 cut(s) 104, 147
EaeI YGGCCR 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 118
FaeI CATG 2 cut(s) 158, 191
FaiI YATR 4 cut(s) 26, 156, 189, 207
FatI CATG 2 cut(s) 154, 187
FokI GGATG 3 cut(s) 42, 96, 209
HaeIII GGCC 1 cut(s) 147
Hin1II CATG 2 cut(s) 158, 191
HinfI GANTC 2 cut(s) 177, 184
HphI GGTGA 1 cut(s) 227
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 2 cut(s) 181, 188
HpyAV CCTTC 1 cut(s) 185
HpyCH4V TGCA 2 cut(s) 57, 209
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
Hsp92II CATG 2 cut(s) 158, 191
LpnPI CCDG 4 cut(s) 21, 132, 141, 166
LweI GCATC 2 cut(s) 66, 231
MfeI CAATTG 1 cut(s) 242
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 2 cut(s) 21, 242
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 1 cut(s) 186
MnlI CCTC 3 cut(s) 67, 167, 236
Mox20I TGGCCA 1 cut(s) 147
MscI TGGCCA 1 cut(s) 147
Msp20I TGGCCA 1 cut(s) 147
MunI CAATTG 1 cut(s) 242
MwoI GCNNNNNNNGC 1 cut(s) 14
NlaIII CATG 2 cut(s) 158, 191
NspI RCATGY 1 cut(s) 158
PagI TCATGA 1 cut(s) 187
PfeI GAWTC 1 cut(s) 184
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
SchI GAGTC 1 cut(s) 186
SetI ASST 2 cut(s) 78, 106
SfaNI GCATC 2 cut(s) 66, 231
SgeI CNNG 7 cut(s) 48, 126, 131, 140, 167, 193, 200
Sse9I AATT 2 cut(s) 21, 242
TasI AATT 2 cut(s) 21, 242
TfiI GAWTC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 176, 204
XceI RCATGY 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.