Rw5G045270

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001631
Physical Location & Seq
Reverse (-)
79161989 .. 79163376
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045270.1

Sequence Viewer

Length: 528 bp
ATGCAGGGGTGGGGACACACGGCAAATATGGCTTTCTTAGCAACTGCTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAGTGGAGACCTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAACGTCGAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGACAACGTTTTGGTTAGAGAGCTTGTCGGAACCAGAGTCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGAAGTGCTTTGCTTGGAGGGATTTTGGCACAGTTGGCTCAAAACATGGGATGGGCAGGGATTGTGATAAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTGAGAGCTTTGGCATCTCATCCCGTGAAGCCCGGCAAAAGAGGCCATGGCAGTAAGCATGTTCCGATCAACATTGCAGGAACCTTGATCCGCGAAGGGGAATGGTTGTATGCTGATAGTGATGGCATCCTTGTCTCCACATCTGAATTGTCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

175

Amino Acids

18.55

Weight (kDa)

6.06

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 15 - 168 3.8e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 99
AccII CGCG 1 cut(s) 462
AciI CCGC 1 cut(s) 460
AclI AACGTT 1 cut(s) 180
AclWI GGATC 1 cut(s) 451
AcuI CTGAAG 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 466
AluBI AGCT 3 cut(s) 71, 196, 377
AluI AGCT 3 cut(s) 71, 196, 377
Alw26I GTCTC 2 cut(s) 81, 508
AlwI GGATC 1 cut(s) 451
AoxI GGCC 2 cut(s) 145, 412
ApeKI GCWGC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 1 cut(s) 402
AsuHPI GGTGA 1 cut(s) 382
BbvI GCAGC 1 cut(s) 314
BccI CCATC 3 cut(s) 230, 297, 485
BceAI ACGGC 1 cut(s) 36
BcnI CCSGG 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 81, 508
BfuAI ACCTGC 1 cut(s) 99
BisI GCNGC 1 cut(s) 328
BlsI GCNGC 1 cut(s) 329
Bme1390I CCNGG 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 3 cut(s) 148, 205, 451
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 2 cut(s) 392, 504
BpuMI CCSGG 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 415
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse3DI GCAATG 1 cut(s) 441
BseDI CCNNGG 1 cut(s) 415
BseGI GGATG 4 cut(s) 308, 362, 388, 495
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 2 cut(s) 147, 414
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 81, 508
BsmFI GGGAC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 137
BsnI GGCC 2 cut(s) 147, 414
Bso31I GGTCTC 1 cut(s) 81
Bsp143I GATC 3 cut(s) 348, 435, 456
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 1 cut(s) 460
BspANI GGCC 2 cut(s) 147, 414
BspCNI CTCAG 1 cut(s) 155
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 148, 205, 451
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 441
BssECI CCNNGG 1 cut(s) 415
BssMI GATC 3 cut(s) 348, 435, 456
BssT1I CCWWGG 1 cut(s) 415
Bst4CI ACNGT 2 cut(s) 160, 285
Bst6I CTCTTC 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 37, 142
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 4 cut(s) 308, 362, 388, 495
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 3 cut(s) 351, 438, 459
BstMAI GTCTC 2 cut(s) 81, 508
BstMBI GATC 3 cut(s) 348, 435, 456
BstMWI GCNNNNNNNGC 5 cut(s) 29, 38, 100, 287, 411
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 400
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 314
BsuRI GGCC 2 cut(s) 147, 414
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 4 cut(s) 308, 362, 388, 495
BtsIMutI CAGTG 1 cut(s) 156
BveI ACCTGC 1 cut(s) 99
Cfr13I GGNCC 1 cut(s) 146
CviAII CATG 4 cut(s) 250, 298, 416, 428
CviJI RGCY 9 cut(s) 32, 71, 147, 196, 290, 327, 377, 400, 414
CviKI_1 RGCY 9 cut(s) 32, 71, 147, 196, 290, 327, 377, 400, 414
DdeI CTNAG 2 cut(s) 37, 142
DpnI GATC 3 cut(s) 350, 437, 458
DpnII GATC 3 cut(s) 348, 435, 456
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 4 cut(s) 253, 301, 419, 431
FaiI YATR 7 cut(s) 29, 123, 251, 299, 417, 429, 480
FaqI GGGAC 1 cut(s) 27
FatI CATG 4 cut(s) 249, 297, 415, 427
Fnu4HI GCNGC 1 cut(s) 328
FokI GGATG 4 cut(s) 315, 369, 375, 482
Fsp4HI GCNGC 1 cut(s) 328
GluI GCNGC 1 cut(s) 328
HaeIII GGCC 2 cut(s) 147, 414
HapII CCGG 1 cut(s) 402
Hin1II CATG 4 cut(s) 253, 301, 419, 431
HinfI GANTC 1 cut(s) 210
HpaII CCGG 1 cut(s) 402
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 5 cut(s) 117, 203, 435, 514, 527
Hpy99I CGWCG 1 cut(s) 135
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 2 cut(s) 160, 285
HpyCH4IV ACGT 2 cut(s) 130, 180
HpyCH4V TGCA 3 cut(s) 4, 330, 446
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 38, 100, 287, 411
HpyF3I CTNAG 2 cut(s) 37, 142
HpySE526I ACGT 2 cut(s) 130, 180
Hsp92II CATG 4 cut(s) 253, 301, 419, 431
Kzo9I GATC 3 cut(s) 348, 435, 456
LpnPI CCDG 7 cut(s) 104, 113, 129, 220, 294, 415, 432
Lsp1109I GCAGC 1 cut(s) 314
LweI GCATC 2 cut(s) 392, 504
MaeII ACGT 2 cut(s) 130, 180
MalI GATC 3 cut(s) 350, 437, 458
MboI GATC 3 cut(s) 348, 435, 456
MboII GAAGA 1 cut(s) 231
MluCI AATT 1 cut(s) 515
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 5 cut(s) 166, 208, 236, 263, 404
MseI TTAA 1 cut(s) 162
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 137
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 5 cut(s) 29, 38, 100, 287, 411
NciI CCSGG 1 cut(s) 402
NcoI CCATGG 1 cut(s) 415
NdeII GATC 3 cut(s) 348, 435, 456
NlaIII CATG 4 cut(s) 253, 301, 419, 431
NlaIV GGNNCC 3 cut(s) 148, 205, 451
NspI RCATGY 1 cut(s) 431
PctI GAATGC 1 cut(s) 137
PflFI GACNNNGTC 1 cut(s) 129
PkrI GCNGC 1 cut(s) 329
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp1406I AACGTT 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 148, 205, 451
PspPI GGNCC 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 1 cut(s) 328
Sau3AI GATC 3 cut(s) 348, 435, 456
Sau96I GGNCC 1 cut(s) 146
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 402
SetI ASST 8 cut(s) 73, 93, 133, 168, 183, 198, 379, 455
SfaNI GCATC 2 cut(s) 392, 504
Sse9I AATT 1 cut(s) 515
SsiI CCGC 1 cut(s) 460
SspI AATATT 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 2 cut(s) 160, 285
TaiI ACGT 2 cut(s) 133, 183
TaqI TCGA 2 cut(s) 133, 213
TasI AATT 1 cut(s) 515
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 1 cut(s) 163
TseI GCWGC 1 cut(s) 327
TspRI CASTG 1 cut(s) 163
Tth111I GACNNNGTC 1 cut(s) 129
XceI RCATGY 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.