RchiOBHm_Chr5g0074341

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001631
Physical Location & Seq
Reverse (-)
80282547 .. 80285417
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34916

Sequence Viewer

Length: 501 bp
ATGGCTTTCTTAGCAACTGCTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAGTGGAGACCTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAAAGTCGAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGATAACGTTTTAGTTAGAGAGCTTCTCGAAACCAGAGGCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGGTGTGCTTTAGTTGGAGGGAACTTGGGACAGTTGGCTCAAAACATGGGATGGGCAGGGATTGTGATAAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTGAGAGCTTTGGCATCTCATCCCATGAAGTCCAACAAACGAGGCCATGGCGATAAACATGTTCCGATCAACATTGCAGGAACCTTGATCGGCGAAGGGGAATGGTTGTATGCTGATAGTGATGGCATTCTTATCTCAACATCTGAACTGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

166

Amino Acids

17.55

Weight (kDa)

4.96

Isoelectric Point (pI)

23.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 1.2e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 72
AclI AACGTT 1 cut(s) 153
AcuI CTGAAG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 400
AluBI AGCT 3 cut(s) 44, 169, 350
AluI AGCT 3 cut(s) 44, 169, 350
Alw26I GTCTC 1 cut(s) 54
AoxI GGCC 2 cut(s) 118, 385
ApeKI GCWGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 355
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 203, 270, 458
BcoDI GTCTC 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 72
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 121, 424
BmsI GCATC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 156, 188
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 388
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 3 cut(s) 281, 335, 361
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 287
BshFI GGCC 2 cut(s) 120, 387
BslFI GGGAC 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 267
BsmI GAATGC 2 cut(s) 110, 468
BsnI GGCC 2 cut(s) 120, 387
Bso31I GGTCTC 1 cut(s) 54
Bsp143I GATC 3 cut(s) 321, 408, 429
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 2 cut(s) 120, 387
BspCNI CTCAG 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 121, 424
BspMI ACCTGC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 414
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 3 cut(s) 321, 408, 429
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 3 cut(s) 133, 258, 492
Bst6I CTCTTC 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 10, 115
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 3 cut(s) 281, 335, 361
BstKTI GATC 3 cut(s) 324, 411, 432
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 3 cut(s) 321, 408, 429
BstMWI GCNNNNNNNGC 2 cut(s) 11, 73
BstNSI RCATGY 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 120, 387
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 3 cut(s) 281, 335, 361
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 119
CviAII CATG 5 cut(s) 223, 271, 367, 389, 401
CviJI RGCY 8 cut(s) 5, 44, 120, 169, 263, 300, 350, 387
CviKI_1 RGCY 8 cut(s) 5, 44, 120, 169, 263, 300, 350, 387
DdeI CTNAG 2 cut(s) 10, 115
DpnI GATC 3 cut(s) 323, 410, 431
DpnII GATC 3 cut(s) 321, 408, 429
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 5 cut(s) 226, 274, 370, 392, 404
FaiI YATR 7 cut(s) 96, 224, 272, 368, 390, 402, 453
FaqI GGGAC 1 cut(s) 267
FatI CATG 5 cut(s) 222, 270, 366, 388, 400
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 3 cut(s) 288, 342, 348
Fsp4HI GCNGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 120, 387
Hin1II CATG 5 cut(s) 226, 274, 370, 392, 404
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 3 cut(s) 90, 408, 487
Hpy188III TCNNGA 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 3 cut(s) 133, 258, 492
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 303, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 73
HpyF3I CTNAG 2 cut(s) 10, 115
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 5 cut(s) 226, 274, 370, 392, 404
Kzo9I GATC 3 cut(s) 321, 408, 429
LpnPI CCDG 6 cut(s) 77, 86, 102, 193, 267, 405
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 365
MaeII ACGT 1 cut(s) 153
MalI GATC 3 cut(s) 323, 410, 431
MboI GATC 3 cut(s) 321, 408, 429
MboII GAAGA 1 cut(s) 204
MmeI TCCRAC 2 cut(s) 220, 399
MnlI CCTC 7 cut(s) 139, 176, 181, 209, 219, 236, 377
MseI TTAA 1 cut(s) 135
Mva1269I GAATGC 2 cut(s) 110, 468
MwoI GCNNNNNNNGC 2 cut(s) 11, 73
NcoI CCATGG 1 cut(s) 388
NdeII GATC 3 cut(s) 321, 408, 429
NlaIII CATG 5 cut(s) 226, 274, 370, 392, 404
NlaIV GGNNCC 2 cut(s) 121, 424
NspI RCATGY 1 cut(s) 404
PciI ACATGT 1 cut(s) 400
PctI GAATGC 2 cut(s) 110, 468
PflFI GACNNNGTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 302
PscI ACATGT 1 cut(s) 400
Psp1406I AACGTT 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 121, 424
PspPI GGNCC 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 3 cut(s) 321, 408, 429
Sau96I GGNCC 1 cut(s) 119
SetI ASST 8 cut(s) 46, 66, 141, 156, 171, 230, 352, 428
SfaNI GCATC 1 cut(s) 365
SspI AATATT 1 cut(s) 84
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 3 cut(s) 133, 258, 492
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 106, 174
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 300
TspDTI ATGAA 1 cut(s) 383
TspRI CASTG 1 cut(s) 136
Tth111I GACNNNGTC 1 cut(s) 102
XceI RCATGY 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.