MD03G1044900.v1.1

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001631
Physical Location & Seq
Forward (+)
3590881 .. 3591380
500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1044900.v1.1.491

Sequence Viewer

Length: 444 bp
ATGCTATTGCGCCGTTTGCCACCGATATTCCAGAAATATGGAGAACGTGAAGCGTTCTCAGGCCCCATTGTGACTGTCAAGACGTTTGAAGACAATACTCTGATAGTGGGGCTTCTCGGAACGAAAGGCGAGGGAAGGGTTTTGGTTGTTGATGGTGAAGGAAGCTTGAGGCGTGCCGTTACCGGAGGAATGTTGGCTAAGTGTGCTGAAGTTATGGGATGGTCTGGGATTGTGATAAATGGGTGCATTAGAGATGTTGATGAGATAAATCGATGTGAGATTGGGGTGAGAGCTTTAGCAACTTGTCCTGTGAGACCAATCAAAAGTGGTGGTGGCCAAAAGCATGTTCCTATCAACATTGGAGGAATCTGGATTCAGGATGGACAATGGTTGTATGCAGATGGTGATGGCATCCTTGTCTCCACATCTCAATTATCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

148

Amino Acids

15.76

Weight (kDa)

7.65

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 14 - 140 7.1e-42 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 228
AgsI TTSAA 1 cut(s) 89
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 2 cut(s) 165, 293
AluI AGCT 2 cut(s) 165, 293
Alw26I GTCTC 2 cut(s) 307, 424
AoxI GGCC 2 cut(s) 61, 334
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 3 cut(s) 167, 298, 416
BalI TGGCCA 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 96
BccI CCATC 5 cut(s) 146, 213, 374, 395, 401
BceAI ACGGC 1 cut(s) 161
BcoDI GTCTC 2 cut(s) 307, 424
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 64
BmsI GCATC 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 187
Bsa29I ATCGAT 1 cut(s) 271
BsaI GGTCTC 1 cut(s) 307
BsaWI WCCGGW 1 cut(s) 182
BseCI ATCGAT 1 cut(s) 271
BseGI GGATG 3 cut(s) 224, 385, 411
BseMII CTCAG 1 cut(s) 72
BshFI GGCC 2 cut(s) 63, 336
BshVI ATCGAT 1 cut(s) 271
BsiSI CCGG 1 cut(s) 183
BsmAI GTCTC 2 cut(s) 307, 424
BsnI GGCC 2 cut(s) 63, 336
Bso31I GGTCTC 1 cut(s) 307
BspANI GGCC 2 cut(s) 63, 336
BspCNI CTCAG 1 cut(s) 71
BspDI ATCGAT 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 58, 198
BstF5I GGATG 3 cut(s) 224, 385, 411
BstHHI GCGC 1 cut(s) 12
BstMAI GTCTC 2 cut(s) 307, 424
BstMWI GCNNNNNNNGC 2 cut(s) 16, 203
BstNSI RCATGY 1 cut(s) 347
BstV2I GAAGAC 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 271
BsuRI GGCC 2 cut(s) 63, 336
BsuTUI ATCGAT 1 cut(s) 271
BtsCI GGATG 3 cut(s) 224, 385, 411
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 1 cut(s) 12
Cfr13I GGNCC 1 cut(s) 62
ClaI ATCGAT 1 cut(s) 271
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviAII CATG 1 cut(s) 344
CviJI RGCY 6 cut(s) 63, 112, 165, 197, 293, 336
CviKI_1 RGCY 6 cut(s) 63, 112, 165, 197, 293, 336
DdeI CTNAG 2 cut(s) 58, 198
EaeI YGGCCR 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 62
FaeI CATG 1 cut(s) 347
FaiI YATR 4 cut(s) 39, 215, 345, 396
FatI CATG 1 cut(s) 343
FokI GGATG 3 cut(s) 231, 392, 398
GlaI GCGC 1 cut(s) 11
HaeIII GGCC 2 cut(s) 63, 336
HapII CCGG 1 cut(s) 183
HhaI GCGC 1 cut(s) 12
Hin1II CATG 1 cut(s) 347
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 2 cut(s) 366, 373
HpaII CCGG 1 cut(s) 183
HphI GGTGA 3 cut(s) 167, 298, 416
Hpy188I TCNGA 2 cut(s) 102, 119
Hpy188III TCNNGA 4 cut(s) 31, 79, 370, 377
HpyAV CCTTC 2 cut(s) 129, 152
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 2 cut(s) 46, 83
HpyCH4V TGCA 2 cut(s) 246, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 203
HpyF3I CTNAG 2 cut(s) 58, 198
HpySE526I ACGT 2 cut(s) 46, 83
Hsp92II CATG 1 cut(s) 347
HspAI GCGC 1 cut(s) 10
LpnPI CCDG 7 cut(s) 44, 45, 196, 210, 321, 355, 362
LweI GCATC 1 cut(s) 420
MaeII ACGT 2 cut(s) 46, 83
MaeIII GTNAC 2 cut(s) 70, 178
MboII GAAGA 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 1 cut(s) 431
MluNI TGGCCA 1 cut(s) 336
MnlI CCTC 4 cut(s) 124, 162, 179, 356
Mox20I TGGCCA 1 cut(s) 336
MscI TGGCCA 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 336
MspI CCGG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 16, 203
NlaIII CATG 1 cut(s) 347
NlaIV GGNNCC 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 70
NspI RCATGY 1 cut(s) 347
PfeI GAWTC 2 cut(s) 366, 373
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 1 cut(s) 62
Sau96I GGNCC 1 cut(s) 62
SetI ASST 4 cut(s) 49, 86, 167, 295
SfaNI GCATC 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 1 cut(s) 431
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 2 cut(s) 49, 86
TaqI TCGA 1 cut(s) 271
TasI AATT 1 cut(s) 431
TfiI GAWTC 2 cut(s) 366, 373
TseFI GTSAC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 70
XceI RCATGY 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.