Rh5AG475600

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001631
Physical Location & Seq
Forward (+)
81640376 .. 81641335
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG475600.1

Sequence Viewer

Length: 690 bp
ATGGAGTCTGATATAACGATCGATTCAGTTCCGGCTCTCGAAATCGACCATGGATATTCATACACCACTCTCACCGTAGAGAAAGTAAGGAGTATAGTAGATTTCAAGCTCAAGAAGTGCGAGATCCTCGAGAAGAGATTCGCTGTCGCCGGGGTGTCAGCCGAGTACAGGAGAAGTCTGGCTGCCGCCGCGCTGGCAACCACAGACGTTTGTGATGCAAATCCCGGACTGTTGGAGAGCGGAGACTGGCGTCTTTTGGCGCCAATCTTTCAAAAATTTGGAAAATGGCGCGCGTTTTCGGGTCAGGTGGTGACCGTCAAGTTGTTTGAGGACAACACTTTGGTGATCGAGGCTCTGTTGAGAAAAGGTGAGGGGAGGGTGTTGGTTGTGGACGGCGGAGGAAGCATGAGTCGTGCCGTCACTGGAGGGATGCTGGCCAAGTGTGCCGAGATTATGGGGTGGGCTGGGATTGTGGTGAATGGTTGCGTCAGAGATGTGGATGAGATCAATGAATGTGAGATCGGGGTGAGAGCTTTGGGGACTTGTCCTGTGAGACCGAAAAGAAGTAAAGATGTGAGTGGCCACAAGCATGTTGATGTCCACATTGGAGGAAAATGGATTCATGATGGGGAATGGTTGTACGCAGATATGGATGGCATCCTCGTCTCCAAGTCTCAATTGTCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

229

Amino Acids

24.92

Weight (kDa)

5.99

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 67 - 222 1.4e-41 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 259
AccBSI CCGCTC 1 cut(s) 240
AccII CGCG 3 cut(s) 191, 291, 293
AciI CCGC 4 cut(s) 186, 189, 240, 396
AclWI GGATC 1 cut(s) 118
AcoI YGGCCR 2 cut(s) 435, 580
AcsI RAATTY 1 cut(s) 275
AcyI GRCGYC 2 cut(s) 250, 260
AfaI GTAC 2 cut(s) 167, 641
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 2 cut(s) 106, 272
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 2 cut(s) 109, 533
AluI AGCT 2 cut(s) 109, 533
Alw26I GTCTC 4 cut(s) 237, 547, 670, 678
AlwI GGATC 1 cut(s) 118
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 435, 580
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 4 cut(s) 193, 262, 291, 293
AsuC2I CCSGG 2 cut(s) 151, 225
AsuHPI GGTGA 6 cut(s) 64, 322, 355, 380, 487, 538
AvaI CYCGRG 1 cut(s) 128
BalI TGGCCA 2 cut(s) 437, 582
BanI GGYRCC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 169
BccI CCATC 2 cut(s) 620, 647
BceAI ACGGC 2 cut(s) 401, 409
BcnI CCSGG 2 cut(s) 151, 225
BcoDI GTCTC 4 cut(s) 237, 547, 670, 678
BfoI RGCGCY 1 cut(s) 263
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 3 cut(s) 183, 186, 189
BlsI GCNGC 3 cut(s) 184, 187, 190
Bme1390I CCNGG 2 cut(s) 151, 225
BmeT110I CYCGRG 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 2 cut(s) 151, 225
BmsI GCATC 3 cut(s) 205, 420, 666
BoxI GACNNNNGTC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 151, 225
Bsa29I ATCGAT 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 2 cut(s) 250, 260
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 2 cut(s) 49, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 251, 427
Bse8I GATNNNNATC 1 cut(s) 219
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 2 cut(s) 49, 150
BseGI GGATG 4 cut(s) 435, 505, 657, 658
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 251, 427
BsePI GCGCGC 1 cut(s) 289
BseXI GCAGC 1 cut(s) 169
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 3 cut(s) 191, 291, 293
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 2 cut(s) 437, 582
BshNI GGYRCC 1 cut(s) 259
BshVI ATCGAT 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 3 cut(s) 32, 150, 225
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 4 cut(s) 237, 547, 670, 678
BsmBI CGTCTC 1 cut(s) 670
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 2 cut(s) 437, 582
Bso31I GGTCTC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 128
Bsp143I GATC 5 cut(s) 18, 123, 345, 504, 519
Bsp19I CCATGG 1 cut(s) 49
BspACI CCGC 4 cut(s) 186, 189, 240, 396
BspANI GGCC 2 cut(s) 437, 582
BspDI ATCGAT 1 cut(s) 21
BspFNI CGCG 3 cut(s) 191, 291, 293
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 1 cut(s) 261
BspPI GGATC 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 547
BsrBI CCGCTC 1 cut(s) 240
BsrI ACTGG 2 cut(s) 251, 427
BssECI CCNNGG 2 cut(s) 49, 150
BssHII GCGCGC 1 cut(s) 289
BssMI GATC 5 cut(s) 18, 123, 345, 504, 519
BssNI GRCGYC 2 cut(s) 250, 260
BssT1I CCWWGG 1 cut(s) 49
Bst4CI ACNGT 3 cut(s) 76, 231, 316
Bst6I CTCTTC 1 cut(s) 128
BstACI GRCGYC 2 cut(s) 250, 260
BstC8I GCNNGC 3 cut(s) 195, 291, 435
BstDSI CCRYGG 1 cut(s) 49
BstEII GGTNACC 1 cut(s) 310
BstF5I GGATG 4 cut(s) 435, 505, 657, 658
BstFNI CGCG 3 cut(s) 191, 291, 293
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 4 cut(s) 193, 262, 291, 293
BstKTI GATC 5 cut(s) 21, 126, 348, 507, 522
BstMAI GTCTC 4 cut(s) 237, 547, 670, 678
BstMBI GATC 5 cut(s) 18, 123, 345, 504, 519
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 4 cut(s) 188, 194, 402, 443
BstNSI RCATGY 1 cut(s) 593
BstPAI GACNNNNGTC 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 149, 223
BstUI CGCG 3 cut(s) 191, 291, 293
BstV1I GCAGC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 2 cut(s) 437, 582
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 4 cut(s) 435, 505, 657, 658
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 3 cut(s) 195, 291, 435
CciI TCATGA 1 cut(s) 622
CfoI GCGC 4 cut(s) 193, 262, 291, 293
ClaI ATCGAT 1 cut(s) 21
CseI GACGC 2 cut(s) 239, 475
Csp6I GTAC 2 cut(s) 166, 640
CviAII CATG 4 cut(s) 50, 406, 590, 623
CviJI RGCY 9 cut(s) 35, 109, 161, 182, 353, 437, 464, 533, 582
CviKI_1 RGCY 9 cut(s) 35, 109, 161, 182, 353, 437, 464, 533, 582
CviQI GTAC 2 cut(s) 166, 640
DinI GGCGCC 1 cut(s) 261
DpnI GATC 5 cut(s) 20, 125, 347, 506, 521
DpnII GATC 5 cut(s) 18, 123, 345, 504, 519
EaeI YGGCCR 2 cut(s) 435, 580
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
EciI GGCGGA 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 547
Eco88I CYCGRG 1 cut(s) 128
Eco91I GGTNACC 1 cut(s) 310
EcoO65I GGTNACC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 49
EgeI GGCGCC 1 cut(s) 261
EheI GGCGCC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 49
Esp3I CGTCTC 1 cut(s) 670
FaeI CATG 4 cut(s) 53, 409, 593, 626
FaiI YATR 9 cut(s) 14, 51, 61, 95, 407, 455, 591, 624, 650
FaqI GGGAC 1 cut(s) 553
FatI CATG 4 cut(s) 49, 405, 589, 622
Fnu4HI GCNGC 3 cut(s) 183, 186, 189
FokI GGATG 4 cut(s) 442, 512, 644, 665
Fsp4HI GCNGC 3 cut(s) 183, 186, 189
GlaI GCGC 4 cut(s) 192, 261, 290, 292
GluI GCNGC 3 cut(s) 183, 186, 189
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 2 cut(s) 437, 582
HapII CCGG 3 cut(s) 32, 150, 225
HgaI GACGC 2 cut(s) 239, 475
HhaI GCGC 4 cut(s) 193, 262, 291, 293
Hin1I GRCGYC 2 cut(s) 250, 260
Hin1II CATG 4 cut(s) 53, 409, 593, 626
Hin6I GCGC 4 cut(s) 191, 260, 289, 291
HinP1I GCGC 4 cut(s) 191, 260, 289, 291
HinfI GANTC 5 cut(s) 5, 23, 138, 409, 619
HpaII CCGG 3 cut(s) 32, 150, 225
HphI GGTGA 6 cut(s) 64, 322, 355, 380, 487, 538
Hpy166II GTNNAC 2 cut(s) 391, 601
Hpy188I TCNGA 2 cut(s) 10, 491
Hpy188III TCNNGA 4 cut(s) 38, 112, 130, 623
Hpy8I GTNNAC 2 cut(s) 391, 601
HpyCH4III ACNGT 3 cut(s) 76, 231, 316
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 1 cut(s) 218
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 194, 402, 443
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 2 cut(s) 250, 260
Hsp92II CATG 4 cut(s) 53, 409, 593, 626
HspAI GCGC 4 cut(s) 191, 260, 289, 291
KasI GGCGCC 1 cut(s) 259
Kzo9I GATC 5 cut(s) 18, 123, 345, 504, 519
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 3 cut(s) 205, 420, 666
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 2 cut(s) 310, 418
MalI GATC 5 cut(s) 20, 125, 347, 506, 521
MbiI CCGCTC 1 cut(s) 240
MboI GATC 5 cut(s) 18, 123, 345, 504, 519
MboII GAAGA 1 cut(s) 145
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 1 cut(s) 123
MlsI TGGCCA 2 cut(s) 437, 582
MluCI AATT 2 cut(s) 275, 677
MluNI TGGCCA 2 cut(s) 437, 582
Mly113I GGCGCC 1 cut(s) 260
MlyI GAGTC 2 cut(s) 14, 418
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 9 cut(s) 137, 322, 343, 364, 369, 392, 419, 602, 671
Mox20I TGGCCA 2 cut(s) 437, 582
MroXI GAANNNNTTC 1 cut(s) 137
MscI TGGCCA 2 cut(s) 437, 582
MslI CAYNNNNRTG 3 cut(s) 341, 588, 594
Msp20I TGGCCA 2 cut(s) 437, 582
MspI CCGG 3 cut(s) 32, 150, 225
MspR9I CCNGG 2 cut(s) 151, 225
MunI CAATTG 1 cut(s) 677
MvnI CGCG 3 cut(s) 191, 291, 293
MwoI GCNNNNNNNGC 4 cut(s) 188, 194, 402, 443
NarI GGCGCC 1 cut(s) 260
NciI CCSGG 2 cut(s) 151, 225
NcoI CCATGG 1 cut(s) 49
NdeII GATC 5 cut(s) 18, 123, 345, 504, 519
NlaIII CATG 4 cut(s) 53, 409, 593, 626
NlaIV GGNNCC 1 cut(s) 261
NmeAIII GCCGAG 2 cut(s) 187, 472
NmuCI GTSAC 2 cut(s) 310, 418
NspI RCATGY 1 cut(s) 593
OliI CACNNNNGTG 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 1 cut(s) 622
PauI GCGCGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 3 cut(s) 23, 138, 619
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 3 cut(s) 184, 187, 190
Ple19I CGATCG 1 cut(s) 21
PleI GAGTC 2 cut(s) 13, 417
PluTI GGCGCC 1 cut(s) 263
PpsI GAGTC 2 cut(s) 13, 417
PshAI GACNNNNGTC 1 cut(s) 249
PspEI GGTNACC 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 261
PsuI RGATCY 1 cut(s) 123
PteI GCGCGC 1 cut(s) 289
PvuI CGATCG 1 cut(s) 21
RsaI GTAC 2 cut(s) 167, 641
RsaNI GTAC 2 cut(s) 166, 640
RseI CAYNNNNRTG 3 cut(s) 341, 588, 594
SatI GCNGC 3 cut(s) 183, 186, 189
Sau3AI GATC 5 cut(s) 18, 123, 345, 504, 519
SchI GAGTC 2 cut(s) 14, 418
ScrFI CCNGG 2 cut(s) 151, 225
SetI ASST 5 cut(s) 111, 210, 309, 370, 535
SfaNI GCATC 3 cut(s) 205, 420, 666
SfoI GGCGCC 1 cut(s) 261
Sfr274I CTCGAG 1 cut(s) 128
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 3 cut(s) 341, 588, 594
SmlI CTYRAG 2 cut(s) 110, 128
SmoI CTYRAG 2 cut(s) 110, 128
Sse9I AATT 2 cut(s) 275, 677
SsiI CCGC 4 cut(s) 186, 189, 240, 396
SspDI GGCGCC 1 cut(s) 259
StyD4I CCNGG 2 cut(s) 149, 223
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 3 cut(s) 76, 231, 316
TaiI ACGT 1 cut(s) 210
TaqI TCGA 5 cut(s) 21, 39, 45, 129, 348
TaqII GACCGA 1 cut(s) 571
TasI AATT 2 cut(s) 275, 677
TatI WGTACW 1 cut(s) 165
TauI GCSGC 2 cut(s) 188, 191
TfiI GAWTC 3 cut(s) 23, 138, 619
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 2 cut(s) 310, 418
TseI GCWGC 1 cut(s) 182
Tsp45I GTSAC 2 cut(s) 310, 418
TspDTI ATGAA 3 cut(s) 48, 525, 611
TspRI CASTG 1 cut(s) 427
XapI RAATTY 1 cut(s) 275
XceI RCATGY 1 cut(s) 593
XhoI CTCGAG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.