Prupe.6G036700_v2.0.a1

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001631
Physical Location & Seq
Forward (+)
2773155 .. 2773536
382 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G036700.1

Sequence Viewer

Length: 285 bp
ATGCGACGTGCTGTCACCGGAGGGATGCTGGCTCGGTCGGCTGAGATTATGGGATGGGCTGGGATTGTGATAAATGGTTGCGTGAGAGATGTGGATGAGATCAATGAATGTGAGATTGGGTTGAGAGCTTTGGCAACTTGTCCAGTGAGACCTATCAAAAACGACTCTGGGTCTGGCCAAAAGCATGTTCCTGTAAGTATTGGAGGGGTCTTGATTCACGAGGGAGAATGGTTGTATGCGGATGGAGATGGCATCCTTGTCTCCTCATCCGAACTGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

95

Amino Acids

9.92

Weight (kDa)

4.97

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 175
AhdI GACNNNNNGTC 2 cut(s) 11, 169
AjiI CACGTC 1 cut(s) 8
AjuI GAANNNNNNNTTGG 4 cut(s) 99, 131, 171, 203
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 142, 265
AoxI GGCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 7
BalI TGGCCA 1 cut(s) 177
BauI CACGAG 1 cut(s) 218
BccI CCATC 3 cut(s) 48, 236, 242
BcoDI GTCTC 2 cut(s) 142, 265
BmeRI GACNNNNNGTC 2 cut(s) 11, 169
BmgBI CACGTC 1 cut(s) 8
BmsI GCATC 2 cut(s) 15, 261
BsaI GGTCTC 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 143
BseGI GGATG 6 cut(s) 30, 59, 100, 247, 252, 266
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 177
BsiEI CGRYCG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 18
BsmAI GTCTC 2 cut(s) 142, 265
BsnI GGCC 1 cut(s) 177
Bso31I GGTCTC 1 cut(s) 142
Bsp143I GATC 1 cut(s) 99
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 142
BsrI ACTGG 1 cut(s) 143
BssMI GATC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 42
BstF5I GGATG 6 cut(s) 30, 59, 100, 247, 252, 266
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 2 cut(s) 142, 265
BstMBI GATC 1 cut(s) 99
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 188
BsuRI GGCC 1 cut(s) 177
BtrI CACGTC 1 cut(s) 8
BtsCI GGATG 6 cut(s) 30, 59, 100, 247, 252, 266
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 30
CviAII CATG 1 cut(s) 185
CviJI RGCY 5 cut(s) 32, 41, 59, 128, 177
CviKI_1 RGCY 5 cut(s) 32, 41, 59, 128, 177
DdeI CTNAG 1 cut(s) 42
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
DriI GACNNNNNGTC 2 cut(s) 11, 169
EaeI YGGCCR 1 cut(s) 175
Eam1105I GACNNNNNGTC 2 cut(s) 11, 169
Eco31I GGTCTC 1 cut(s) 142
FaeI CATG 1 cut(s) 188
FaiI YATR 3 cut(s) 50, 186, 237
FatI CATG 1 cut(s) 184
FokI GGATG 6 cut(s) 37, 66, 107, 239, 253, 254
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 1 cut(s) 177
HapII CCGG 1 cut(s) 18
Hin1II CATG 1 cut(s) 188
HinfI GANTC 2 cut(s) 164, 214
HpaII CCGG 1 cut(s) 18
HphI GGTGA 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 2 cut(s) 211, 218
Hpy99I CGWCG 1 cut(s) 9
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 7
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 1 cut(s) 188
Kzo9I GATC 1 cut(s) 99
LpnPI CCDG 7 cut(s) 14, 31, 45, 153, 156, 159, 204
LweI GCATC 2 cut(s) 15, 261
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 13
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MlsI TGGCCA 1 cut(s) 177
MluNI TGGCCA 1 cut(s) 177
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 4 cut(s) 14, 197, 214, 274
Mox20I TGGCCA 1 cut(s) 177
MscI TGGCCA 1 cut(s) 177
Msp20I TGGCCA 1 cut(s) 177
MspI CCGG 1 cut(s) 18
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 1 cut(s) 99
NlaIII CATG 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 188
PfeI GAWTC 1 cut(s) 214
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 59
Sau3AI GATC 1 cut(s) 99
SchI GAGTC 1 cut(s) 158
SetI ASST 3 cut(s) 10, 130, 154
SfaNI GCATC 2 cut(s) 15, 261
SsiI CCGC 1 cut(s) 239
TaaI ACNGT 1 cut(s) 276
TaiI ACGT 1 cut(s) 10
TaqII GACCGA 1 cut(s) 24
TfiI GAWTC 1 cut(s) 214
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 1 cut(s) 120
TspRI CASTG 1 cut(s) 150
XceI RCATGY 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.