MD11G1047500.v1.1

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001631
Physical Location & Seq
Forward (+)
4137541 .. 4139681
2141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1047500.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGCTACCTTAGCAACTGCTGAAATTTGTGATACGAATACAACTCTTGTGGGAAGCGGAGGTTTGCGTGTGCTGCCACCAATTTTCCAGGTATATGGACAATCCCGAGCATTCTCAGGCCCCATTACGACACTCAAGGTGTTTGAGGACAATGTCTTAGTCAGACAGCTTCTCGAAACCAAAGGCGAGGGAAGAGTTTTGGTCATTGACGGAGGAGGAAGCATGAGATGTGCTTTGGTTGGAGGGAACTTGGGACAGTTGGCTCAAAACATGGGATGGGCAGGTATTGTGGTGAATGGCTGCATTAGAGATGTAGATGAGATCAATGAGTGTGATATTGGGGTGAGGGCTTTGGCATCTCATCCGATGAAATCGAACAAAAAAGGCGTCGGAGAAAAGCATGTTCCGATCAACATTGCGGGAACCATGATTCGTGAAGGCGAATGGTTGTATGCAGATAACGATGGCGTCCTTATTTCGACATCTGAATTGTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.57

Weight (kDa)

5.15

Isoelectric Point (pI)

23.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 1.6e-47 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AciI CCGC 2 cut(s) 57, 419
AcsI RAATTY 1 cut(s) 24
AcyI GRCGYC 2 cut(s) 387, 468
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 2 cut(s) 73, 300
ApoI RAATTY 1 cut(s) 24
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 304, 355
AvaI CYCGRG 1 cut(s) 105
BbvI GCAGC 2 cut(s) 60, 287
BccI CCATC 2 cut(s) 270, 458
BciT130I CCWGG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 2 cut(s) 74, 301
BlsI GCNGC 2 cut(s) 75, 302
Bme1390I CCNGG 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 121, 424
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 119
BsaHI GRCGYC 2 cut(s) 387, 468
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 89
BseGI GGATG 2 cut(s) 281, 361
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 129
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 2 cut(s) 60, 287
BshFI GGCC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 267
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 1 cut(s) 120
BsoBI CYCGRG 1 cut(s) 105
Bsp143I GATC 2 cut(s) 321, 408
BspACI CCGC 2 cut(s) 57, 419
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 121, 424
BspMI ACCTGC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 414
BssMI GATC 2 cut(s) 321, 408
BssNI GRCGYC 2 cut(s) 387, 468
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 187
BstACI GRCGYC 2 cut(s) 387, 468
BstDEI CTNAG 3 cut(s) 10, 115, 157
BstF5I GGATG 2 cut(s) 281, 361
BstKTI GATC 2 cut(s) 324, 411
BstMBI GATC 2 cut(s) 321, 408
BstMWI GCNNNNNNNGC 2 cut(s) 11, 73
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 60, 287
BstXI CCANNNNNNTGG 1 cut(s) 95
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 2 cut(s) 281, 361
BveI ACCTGC 1 cut(s) 272
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 2 cut(s) 376, 457
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 4 cut(s) 223, 271, 401, 427
CviJI RGCY 6 cut(s) 5, 120, 169, 263, 300, 350
CviKI_1 RGCY 6 cut(s) 5, 120, 169, 263, 300, 350
DdeI CTNAG 3 cut(s) 10, 115, 157
DpnI GATC 2 cut(s) 323, 410
DpnII GATC 2 cut(s) 321, 408
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 87
FaeI CATG 4 cut(s) 226, 274, 404, 430
FaiI YATR 7 cut(s) 94, 96, 224, 272, 402, 428, 453
FaqI GGGAC 1 cut(s) 267
FatI CATG 4 cut(s) 222, 270, 400, 426
FauI CCCGC 1 cut(s) 412
Fnu4HI GCNGC 2 cut(s) 74, 301
FokI GGATG 2 cut(s) 288, 348
Fsp4HI GCNGC 2 cut(s) 74, 301
GluI GCNGC 2 cut(s) 74, 301
HaeIII GGCC 1 cut(s) 120
HgaI GACGC 2 cut(s) 376, 457
Hin1I GRCGYC 2 cut(s) 387, 468
Hin1II CATG 4 cut(s) 226, 274, 404, 430
HinfI GANTC 1 cut(s) 430
HphI GGTGA 2 cut(s) 304, 355
Hpy188I TCNGA 5 cut(s) 164, 366, 392, 408, 487
Hpy188III TCNNGA 3 cut(s) 105, 173, 434
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 303, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 73
HpyF3I CTNAG 3 cut(s) 10, 115, 157
Hsp92I GRCGYC 2 cut(s) 387, 468
Hsp92II CATG 4 cut(s) 226, 274, 404, 430
Kzo9I GATC 2 cut(s) 321, 408
LpnPI CCDG 4 cut(s) 74, 101, 102, 267
Lsp1109I GCAGC 2 cut(s) 60, 287
LweI GCATC 1 cut(s) 365
MalI GATC 2 cut(s) 323, 410
MboI GATC 2 cut(s) 321, 408
MboII GAAGA 1 cut(s) 204
MluCI AATT 3 cut(s) 24, 81, 488
MmeI TCCRAC 2 cut(s) 220, 370
MnlI CCTC 7 cut(s) 53, 139, 181, 206, 209, 236, 339
MspR9I CCNGG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 110
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 11, 73
NdeII GATC 2 cut(s) 321, 408
NlaIII CATG 4 cut(s) 226, 274, 404, 430
NlaIV GGNNCC 2 cut(s) 121, 424
NspI RCATGY 1 cut(s) 404
PctI GAATGC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 430
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 75, 302
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 121, 424
PspPI GGNCC 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 152
SatI GCNGC 2 cut(s) 74, 301
Sau3AI GATC 2 cut(s) 321, 408
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 89
SetI ASST 6 cut(s) 11, 64, 93, 141, 171, 286
SfaNI GCATC 1 cut(s) 365
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 3 cut(s) 24, 81, 488
SsiI CCGC 2 cut(s) 57, 419
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 174, 374, 479
TasI AATT 3 cut(s) 24, 81, 488
TfiI GAWTC 1 cut(s) 430
TseI GCWGC 2 cut(s) 73, 300
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 225
Tth111I GACNNNGTC 1 cut(s) 152
XapI RAATTY 1 cut(s) 24
XceI RCATGY 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.