RLG00000036469

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001631
Physical Location & Seq
Reverse (-)
81005928 .. 81007294
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036469

Sequence Viewer

Length: 666 bp
ATGGCTTTCTTAGCAACTGCTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAATGGAGATTTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAACGTCAAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGACAACGTTTTGGTTAGAGAGCTTGTCAGAACCAGAGTCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGAAGTGCTTTGCTTGGAGGGAAATTGGCACAGTTGGCTCAAAACATGGGATGGGCAGGGATTGTGATAAATGGCTGCATTAGAGATGTAGATGACATCAATGGATGTGATATTGGGGTGAGAGCTTTGGCATCTCATCCTGTGAAGCCCGGCAAAAGAGGCCATGGCGATAAGCATGTTCCGATCAACATTGCAGGAACCTTGATCTGCGAAGGGGAATGGTTGTATGCTGATAGTGATGGCATCCTTATCTCCACATCTGAATTTGATCTGTGCATTCATCAATTTTCGGTACCAGAAAGTACTTGCAGTTTGTCTCCTTCCTCATCAAAAGCGAAGTTGAAGGCATATGTTGATCGAGCTTCATGGAGTGGAACAGACATTGCATCCTGGTTCTCTGTTGCTTATGGAATCACTGCGATGAGTAGCTATCCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

222

Amino Acids

23.6

Weight (kDa)

5.78

Isoelectric Point (pI)

27.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 1e-43 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 517
AclI AACGTT 1 cut(s) 153
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 519, 529
AgsI TTSAA 1 cut(s) 568
AjnI CCWGG 1 cut(s) 614
AluBI AGCT 5 cut(s) 44, 169, 350, 587, 654
AluI AGCT 5 cut(s) 44, 169, 350, 587, 654
Alw26I GTCTC 1 cut(s) 546
AoxI GGCC 2 cut(s) 118, 385
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 488
Asp718I GGTACC 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 355
BanI GGYRCC 1 cut(s) 517
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 203, 270, 458
BciT130I CCWGG 1 cut(s) 616
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 546
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 375, 616
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 3 cut(s) 121, 424, 519
BmrFI CCNGG 2 cut(s) 375, 616
BmsI GCATC 3 cut(s) 365, 477, 620
BpuMI CCSGG 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 388
Bse3DI GCAATG 2 cut(s) 414, 606
BseBI CCWGG 1 cut(s) 616
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 5 cut(s) 281, 335, 361, 468, 611
BseMI GCAATG 2 cut(s) 414, 606
BseMII CTCAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 287
BshFI GGCC 2 cut(s) 120, 387
BshNI GGYRCC 1 cut(s) 517
BsiSI CCGG 1 cut(s) 375
BsmAI GTCTC 1 cut(s) 546
BsmI GAATGC 2 cut(s) 110, 501
BsnI GGCC 2 cut(s) 120, 387
Bsp143I GATC 4 cut(s) 408, 429, 493, 580
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 2 cut(s) 120, 387
BspCNI CTCAG 1 cut(s) 128
BspLI GGNNCC 3 cut(s) 121, 424, 519
BspT107I GGYRCC 1 cut(s) 517
BsrDI GCAATG 2 cut(s) 414, 606
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 4 cut(s) 408, 429, 493, 580
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 2 cut(s) 133, 258
Bst6I CTCTTC 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 10, 115, 663
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 5 cut(s) 281, 335, 361, 468, 611
BstKTI GATC 4 cut(s) 411, 432, 496, 583
BstMAI GTCTC 1 cut(s) 546
BstMBI GATC 4 cut(s) 408, 429, 493, 580
BstMWI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
BstNI CCWGG 1 cut(s) 616
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 373, 614
BstV1I GCAGC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 120, 387
BtgI CCRYGG 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 659
BtsCI GGATG 5 cut(s) 281, 335, 361, 468, 611
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 2 cut(s) 129, 639
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 518, 528
CviAII CATG 5 cut(s) 223, 271, 389, 401, 591
CviQI GTAC 2 cut(s) 518, 528
DdeI CTNAG 3 cut(s) 10, 115, 663
DpnI GATC 4 cut(s) 410, 431, 495, 582
DpnII GATC 4 cut(s) 408, 429, 493, 580
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 614
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 5 cut(s) 226, 274, 392, 404, 594
FatI CATG 5 cut(s) 222, 270, 388, 400, 590
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 5 cut(s) 288, 342, 348, 455, 598
Fsp4HI GCNGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 120, 387
HapII CCGG 1 cut(s) 375
Hin1II CATG 5 cut(s) 226, 274, 392, 404, 594
HinfI GANTC 2 cut(s) 183, 636
HpaII CCGG 1 cut(s) 375
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 4 cut(s) 90, 176, 408, 487
HpyAV CCTTC 3 cut(s) 431, 555, 562
HpyCH4III ACNGT 2 cut(s) 133, 258
HpyCH4IV ACGT 2 cut(s) 103, 153
HpyCH4V TGCA 5 cut(s) 303, 419, 501, 534, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
HpyF3I CTNAG 3 cut(s) 10, 115, 663
HpySE526I ACGT 2 cut(s) 103, 153
Hsp92II CATG 5 cut(s) 226, 274, 392, 404, 594
KpnI GGTACC 1 cut(s) 521
Kzo9I GATC 4 cut(s) 408, 429, 493, 580
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 3 cut(s) 365, 477, 620
MaeII ACGT 2 cut(s) 103, 153
MalI GATC 4 cut(s) 410, 431, 495, 582
MboI GATC 4 cut(s) 408, 429, 493, 580
MboII GAAGA 1 cut(s) 204
MluCI AATT 3 cut(s) 248, 488, 509
MlyI GAGTC 1 cut(s) 192
MnlI CCTC 6 cut(s) 139, 181, 209, 236, 377, 559
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 1 cut(s) 644
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 2 cut(s) 375, 616
Mva1269I GAATGC 2 cut(s) 110, 501
MvaI CCWGG 1 cut(s) 616
MwoI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 388
NdeI CATATG 1 cut(s) 574
NdeII GATC 4 cut(s) 408, 429, 493, 580
NlaIII CATG 5 cut(s) 226, 274, 392, 404, 594
NlaIV GGNNCC 3 cut(s) 121, 424, 519
NspI RCATGY 1 cut(s) 404
PctI GAATGC 2 cut(s) 110, 501
PfeI GAWTC 1 cut(s) 636
PflFI GACNNNGTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 614
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 3 cut(s) 121, 424, 519
PspPI GGNCC 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 102
RsaI GTAC 2 cut(s) 519, 529
RsaNI GTAC 2 cut(s) 518, 528
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 4 cut(s) 408, 429, 493, 580
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 2 cut(s) 375, 616
SetI ASST 9 cut(s) 46, 106, 141, 156, 171, 352, 428, 589, 656
SfaNI GCATC 3 cut(s) 365, 477, 620
SmiMI CAYNNNNRTG 1 cut(s) 644
Sse9I AATT 3 cut(s) 248, 488, 509
SspI AATATT 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 373, 614
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 133, 258
TaiI ACGT 2 cut(s) 106, 156
TaqI TCGA 2 cut(s) 186, 583
TasI AATT 3 cut(s) 248, 488, 509
TatI WGTACW 1 cut(s) 527
TfiI GAWTC 1 cut(s) 636
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 2 cut(s) 136, 646
TseI GCWGC 1 cut(s) 300
TspDTI ATGAA 2 cut(s) 494, 579
TspRI CASTG 2 cut(s) 136, 646
Tth111I GACNNNGTC 1 cut(s) 102
XapI RAATTY 1 cut(s) 488
XceI RCATGY 1 cut(s) 404
ZrmI AGTACT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.