Rw6G033710

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001631
Physical Location & Seq
Reverse (-)
58312170 .. 58313346
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033710.1

Sequence Viewer

Length: 837 bp
ATGTATTTTTTCTGGAAGACGCTTTTTGAAGATATCTTCAAGCGTTACGAGCTCACTGGCATTGTCAACGGCAGCGAACCCTGTCCCAATCGGTTCCTCATTCATAAGAACGGAAACCTCACGAATCAAGTCAATCCGGCCTTCGATGTTTGGCATGAGAAGGATCAGAGGATCATAACGTGGATCAAATCGACTCTTTCCGAAGACGTGGTGCTCCCACTAGTCACAACCGGAGTCATACCTTCAAATTCGTCTTGTGAGCTTTGGCTTAGCCTCAGGAAGAGATTCGCAGTCGCGGGGGTGTCAGCCGAGTACAGGAGAAGTCTGGCCGCGGCCACCTTGGCAACCACCGACATTTGTGACGCAAATCCCGCTCGGTTGGAGAGCGGGGACTTGCGTCTTTTGGAGCCAAAGTTTCAAAGATTCGGAAAATGGCACGCGTTTTCGGGTCGGGTGCTGACCTTGAAGGTGTTTGAGGACAACACGTTGGTGATTGAAGCTTTGTCGAGAAAAGGGGAGGGGAGGGTGTTGGTTGTGGACGGCGGAGGAAGCATGAGACGCGCCGTCACCGGAGGGATGCTGGCCAAGTGTGCCGAGATTATGGGGTGGGCTGGGATTGTGGTGAATGGGTGCGTAAGAGATGTGGATGAGATCAATGAATGTGAGATCGGGGTGAGAGCTTTGGCGACTTGTCCTGTGAGACCGATTAAAGGTGAAGATGGGAGTGGCCAGAAGCATGTTGATGTCAACATTGGAGGGACATGGATTCGTGACGGGGAATGGTTGTATGCGGATAATGATGGCATCCTTGTCTCCACGACTAAATTGTCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

278

Amino Acids

30.77

Weight (kDa)

6.45

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 116 - 271 5.6e-43 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 826
AccBSI CCGCTC 2 cut(s) 374, 387
AccII CGCG 4 cut(s) 296, 332, 440, 561
AciI CCGC 7 cut(s) 296, 330, 332, 372, 387, 543, 791
AclWI GGATC 3 cut(s) 171, 179, 191
AcoI YGGCCR 4 cut(s) 327, 333, 582, 727
AcsI RAATTY 1 cut(s) 247
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 315, 710
AflIII ACRYGT 2 cut(s) 438, 483
AgsI TTSAA 6 cut(s) 29, 40, 246, 419, 466, 497
AhdI GACNNNNNGTC 1 cut(s) 563
AhlI ACTAGT 1 cut(s) 220
AjiI CACGTC 1 cut(s) 208
AleI CACNNNNGTG 1 cut(s) 488
AluBI AGCT 4 cut(s) 52, 262, 500, 680
AluI AGCT 4 cut(s) 52, 262, 500, 680
Alw21I GWGCWC 2 cut(s) 54, 216
Alw26I GTCTC 3 cut(s) 550, 694, 817
AlwI GGATC 3 cut(s) 171, 179, 191
AoxI GGCC 5 cut(s) 138, 327, 333, 582, 727
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 247
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 563
AsuHPI GGTGA 5 cut(s) 502, 559, 634, 685, 725
AxyI CCTNAGG 1 cut(s) 275
BalI TGGCCA 2 cut(s) 584, 729
BanII GRGCYC 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 23, 210
Bbv12I GWGCWC 2 cut(s) 54, 216
BbvI GCAGC 1 cut(s) 84
BccI CCATC 2 cut(s) 713, 794
BceAI ACGGC 3 cut(s) 85, 548, 556
BcoDI GTCTC 3 cut(s) 550, 694, 817
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 341
BisI GCNGC 3 cut(s) 73, 330, 333
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 3 cut(s) 74, 331, 334
BmeRI GACNNNNNGTC 1 cut(s) 563
BmgBI CACGTC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 95, 408
BmsI GCATC 2 cut(s) 567, 813
BoxI GACNNNNGTC 1 cut(s) 396
BpiI GAAGAC 2 cut(s) 23, 210
Bpu1102I GCTNAGC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 330, 339
BsaWI WCCGGW 2 cut(s) 230, 569
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 315, 710
Bse1I ACTGG 1 cut(s) 61
Bse21I CCTNAGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 330, 339
BseGI GGATG 3 cut(s) 582, 652, 804
BseLI CCNNNNNNNGG 2 cut(s) 315, 710
BseMII CTCAG 1 cut(s) 289
BseNI ACTGG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 84
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 4 cut(s) 296, 332, 440, 561
BshFI GGCC 5 cut(s) 140, 329, 335, 584, 729
BsiHKAI GWGCWC 2 cut(s) 54, 216
BsiSI CCGG 3 cut(s) 137, 231, 570
BslFI GGGAC 3 cut(s) 69, 404, 772
BslI CCNNNNNNNGG 2 cut(s) 315, 710
BsmAI GTCTC 3 cut(s) 550, 694, 817
BsmBI CGTCTC 1 cut(s) 550
BsmFI GGGAC 3 cut(s) 69, 404, 772
BsnI GGCC 5 cut(s) 140, 329, 335, 584, 729
Bso31I GGTCTC 1 cut(s) 694
Bsp1286I GDGCHC 2 cut(s) 54, 216
Bsp143I GATC 5 cut(s) 163, 171, 183, 651, 666
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 7 cut(s) 296, 330, 332, 372, 387, 543, 791
BspANI GGCC 5 cut(s) 140, 329, 335, 584, 729
BspCNI CTCAG 1 cut(s) 288
BspFNI CGCG 4 cut(s) 296, 332, 440, 561
BspLI GGNNCC 2 cut(s) 95, 408
BspPI GGATC 3 cut(s) 171, 179, 191
BspTNI GGTCTC 1 cut(s) 694
BsrBI CCGCTC 2 cut(s) 374, 387
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 330, 339
BssMI GATC 5 cut(s) 163, 171, 183, 651, 666
BssT1I CCWWGG 1 cut(s) 339
Bst6I CTCTTC 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 438, 582
BstDEI CTNAG 2 cut(s) 269, 275
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 582, 652, 804
BstFNI CGCG 4 cut(s) 296, 332, 440, 561
BstHHI GCGC 1 cut(s) 563
BstKTI GATC 5 cut(s) 166, 174, 186, 654, 669
BstMAI GTCTC 3 cut(s) 550, 694, 817
BstMBI GATC 5 cut(s) 163, 171, 183, 651, 666
BstMWI GCNNNNNNNGC 6 cut(s) 49, 341, 371, 549, 558, 590
BstNSI RCATGY 1 cut(s) 740
BstPAI GACNNNNGTC 1 cut(s) 396
BstUI CGCG 4 cut(s) 296, 332, 440, 561
BstV1I GCAGC 1 cut(s) 84
BstV2I GAAGAC 2 cut(s) 23, 210
Bsu36I CCTNAGG 1 cut(s) 275
BsuRI GGCC 5 cut(s) 140, 329, 335, 584, 729
BtgI CCRYGG 1 cut(s) 330
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 3 cut(s) 582, 652, 804
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 2 cut(s) 438, 582
CfoI GCGC 1 cut(s) 563
Cfr42I CCGCGG 1 cut(s) 333
CseI GACGC 4 cut(s) 28, 371, 386, 567
Csp6I GTAC 1 cut(s) 313
CviAII CATG 4 cut(s) 155, 553, 737, 762
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 2 cut(s) 269, 275
DpnI GATC 5 cut(s) 165, 173, 185, 653, 668
DpnII GATC 5 cut(s) 163, 171, 183, 651, 666
DrdI GACNNNNNNGTC 1 cut(s) 826
DriI GACNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 826
EaeI YGGCCR 4 cut(s) 327, 333, 582, 727
Eam1104I CTCTTC 1 cut(s) 275
Eam1105I GACNNNNNGTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 275
EciI GGCGGA 1 cut(s) 558
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 694
Eco32I GATATC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 52
Eco81I CCTNAGG 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 52
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 550
FaeI CATG 4 cut(s) 158, 556, 740, 765
FaiI YATR 9 cut(s) 105, 156, 176, 239, 554, 602, 738, 763, 789
FaqI GGGAC 3 cut(s) 69, 404, 772
FatI CATG 4 cut(s) 154, 552, 736, 761
FauI CCCGC 3 cut(s) 289, 379, 380
Fnu4HI GCNGC 3 cut(s) 73, 330, 333
FokI GGATG 3 cut(s) 589, 659, 791
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 3 cut(s) 73, 330, 333
FspBI CTAG 1 cut(s) 221
GlaI GCGC 1 cut(s) 562
GluI GCNGC 3 cut(s) 73, 330, 333
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 5 cut(s) 140, 329, 335, 584, 729
HapII CCGG 3 cut(s) 137, 231, 570
HgaI GACGC 4 cut(s) 28, 371, 386, 567
HhaI GCGC 1 cut(s) 563
Hin1II CATG 4 cut(s) 158, 556, 740, 765
Hin6I GCGC 1 cut(s) 561
HinP1I GCGC 1 cut(s) 561
HincII GTYRAC 2 cut(s) 67, 748
HindII GTYRAC 2 cut(s) 67, 748
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 6 cut(s) 124, 193, 234, 285, 423, 766
HpaII CCGG 3 cut(s) 137, 231, 570
HphI GGTGA 5 cut(s) 502, 559, 634, 685, 725
Hpy166II GTNNAC 3 cut(s) 67, 538, 748
Hpy188I TCNGA 3 cut(s) 168, 202, 428
Hpy188III TCNNGA 5 cut(s) 13, 121, 277, 507, 770
Hpy8I GTNNAC 3 cut(s) 67, 538, 748
HpyAV CCTTC 4 cut(s) 151, 154, 252, 460
HpyCH4IV ACGT 3 cut(s) 179, 207, 485
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 341, 371, 549, 558, 590
HpyF3I CTNAG 2 cut(s) 269, 275
HpySE526I ACGT 3 cut(s) 179, 207, 485
Hsp92II CATG 4 cut(s) 158, 556, 740, 765
HspAI GCGC 1 cut(s) 561
KspI CCGCGG 1 cut(s) 333
Kzo9I GATC 5 cut(s) 163, 171, 183, 651, 666
LmnI GCTCC 2 cut(s) 219, 406
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 2 cut(s) 567, 813
MaeI CTAG 1 cut(s) 221
MaeII ACGT 3 cut(s) 179, 207, 485
MaeIII GTNAC 5 cut(s) 44, 223, 359, 565, 770
MalI GATC 5 cut(s) 165, 173, 185, 653, 668
MbiI CCGCTC 2 cut(s) 374, 387
MboI GATC 5 cut(s) 163, 171, 183, 651, 666
MboII GAAGA 6 cut(s) 28, 28, 41, 215, 292, 728
MhlI GDGCHC 2 cut(s) 54, 216
MlsI TGGCCA 2 cut(s) 584, 729
MluCI AATT 3 cut(s) 247, 824, 831
MluI ACGCGT 1 cut(s) 438
MluNI TGGCCA 2 cut(s) 584, 729
MlyI GAGTC 2 cut(s) 187, 243
MmeI TCCRAC 1 cut(s) 360
Mox20I TGGCCA 2 cut(s) 584, 729
MroXI GAANNNNTTC 1 cut(s) 284
MscI TGGCCA 2 cut(s) 584, 729
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 2 cut(s) 488, 741
Msp20I TGGCCA 2 cut(s) 584, 729
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 3 cut(s) 137, 231, 570
MvnI CGCG 4 cut(s) 296, 332, 440, 561
MwoI GCNNNNNNNGC 6 cut(s) 49, 341, 371, 549, 558, 590
NdeII GATC 5 cut(s) 163, 171, 183, 651, 666
NlaIII CATG 4 cut(s) 158, 556, 740, 765
NlaIV GGNNCC 2 cut(s) 95, 408
NmeAIII GCCGAG 2 cut(s) 334, 619
NmuCI GTSAC 4 cut(s) 223, 359, 565, 770
NspI RCATGY 1 cut(s) 740
OliI CACNNNNGTG 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 4 cut(s) 124, 285, 423, 766
PkrI GCNGC 3 cut(s) 74, 331, 334
PleI GAGTC 2 cut(s) 187, 242
PpsI GAGTC 2 cut(s) 187, 242
PshAI GACNNNNGTC 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 611
PspN4I GGNNCC 2 cut(s) 95, 408
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 2 cut(s) 488, 741
SacI GAGCTC 1 cut(s) 54
SacII CCGCGG 1 cut(s) 333
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 73, 330, 333
Sau3AI GATC 5 cut(s) 163, 171, 183, 651, 666
SchI GAGTC 2 cut(s) 187, 243
SduI GDGCHC 2 cut(s) 54, 216
SfaNI GCATC 2 cut(s) 567, 813
Sfr303I CCGCGG 1 cut(s) 333
SgrBI CCGCGG 1 cut(s) 333
SmiMI CAYNNNNRTG 2 cut(s) 488, 741
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 3 cut(s) 247, 824, 831
SsiI CCGC 7 cut(s) 296, 330, 332, 372, 387, 543, 791
SspMI CTAG 1 cut(s) 221
SstI GAGCTC 1 cut(s) 54
StyI CCWWGG 1 cut(s) 339
TaiI ACGT 3 cut(s) 182, 210, 488
TaqI TCGA 3 cut(s) 144, 191, 506
TaqII GACCGA 1 cut(s) 718
TasI AATT 3 cut(s) 247, 824, 831
TatI WGTACW 1 cut(s) 312
TauI GCSGC 2 cut(s) 332, 335
TfiI GAWTC 4 cut(s) 124, 285, 423, 766
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 4 cut(s) 223, 359, 565, 770
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 4 cut(s) 223, 359, 565, 770
TspDTI ATGAA 2 cut(s) 92, 672
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 1 cut(s) 61
XapI RAATTY 1 cut(s) 247
XceI RCATGY 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.