Rh5CG532700

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001631
Physical Location & Seq
Reverse (-)
74649367 .. 74649867
501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG532700.1

Sequence Viewer

Length: 501 bp
ATGGCTTTCTTAGCAACTGCTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAGTGGAGATTTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAACGTCGAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGACAACGTTTTGGTTAGAGAGCTTGTCGGAACCAGAGTCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGAAGTGCTTTGCTTGGAGGGATTTTGGCACAGTTGGCTCAAAACATGGGATGGGCAGGGATTGTGATTAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTGAGAGCTTTGGCATCTCATCCCGTGAAGCCCGGCAAAAGAGGCCATGGCAATAAGCATGTTCCAATCAACATTGCAGGAACCTTGATCCGCGAAGGGGAATGGTTGTATGCTGATAGTGATGGCATCCTTGTCTCCACATCTGAATTGTCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

166

Amino Acids

17.6

Weight (kDa)

5.89

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 3.3e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 435
AciI CCGC 1 cut(s) 433
AclI AACGTT 1 cut(s) 153
AclWI GGATC 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 439
AluBI AGCT 3 cut(s) 44, 169, 350
AluI AGCT 3 cut(s) 44, 169, 350
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 1 cut(s) 424
AoxI GGCC 2 cut(s) 118, 385
ApeKI GCWGC 1 cut(s) 300
AseI ATTAAT 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 355
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 203, 270, 458
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 481
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 3 cut(s) 121, 178, 424
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 2 cut(s) 365, 477
BpuMI CCSGG 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 388
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 4 cut(s) 281, 335, 361, 468
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 2 cut(s) 120, 387
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 1 cut(s) 481
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 2 cut(s) 120, 387
Bsp143I GATC 2 cut(s) 321, 429
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 1 cut(s) 433
BspANI GGCC 2 cut(s) 120, 387
BspCNI CTCAG 1 cut(s) 128
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 121, 178, 424
BspPI GGATC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 414
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 2 cut(s) 321, 429
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 133, 258
Bst6I CTCTTC 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 10, 115
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 4 cut(s) 281, 335, 361, 468
BstFNI CGCG 1 cut(s) 435
BstKTI GATC 2 cut(s) 324, 432
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 2 cut(s) 321, 429
BstMWI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 373
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 120, 387
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 4 cut(s) 281, 335, 361, 468
BtsIMutI CAGTG 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 119
CviAII CATG 4 cut(s) 223, 271, 389, 401
CviJI RGCY 9 cut(s) 5, 44, 120, 169, 263, 300, 350, 373, 387
CviKI_1 RGCY 9 cut(s) 5, 44, 120, 169, 263, 300, 350, 373, 387
DdeI CTNAG 2 cut(s) 10, 115
DpnI GATC 2 cut(s) 323, 431
DpnII GATC 2 cut(s) 321, 429
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 4 cut(s) 226, 274, 392, 404
FaiI YATR 6 cut(s) 96, 224, 272, 390, 402, 453
FatI CATG 4 cut(s) 222, 270, 388, 400
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 4 cut(s) 288, 342, 348, 455
Fsp4HI GCNGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 120, 387
HapII CCGG 1 cut(s) 375
Hin1II CATG 4 cut(s) 226, 274, 392, 404
HinfI GANTC 1 cut(s) 183
HpaII CCGG 1 cut(s) 375
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 4 cut(s) 90, 176, 487, 500
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 2 cut(s) 133, 258
HpyCH4IV ACGT 2 cut(s) 103, 153
HpyCH4V TGCA 2 cut(s) 303, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
HpyF3I CTNAG 2 cut(s) 10, 115
HpySE526I ACGT 2 cut(s) 103, 153
Hsp92II CATG 4 cut(s) 226, 274, 392, 404
Kzo9I GATC 2 cut(s) 321, 429
LpnPI CCDG 6 cut(s) 86, 102, 193, 267, 388, 405
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 2 cut(s) 365, 477
MaeII ACGT 2 cut(s) 103, 153
MalI GATC 2 cut(s) 323, 431
MboI GATC 2 cut(s) 321, 429
MboII GAAGA 1 cut(s) 204
MluCI AATT 1 cut(s) 488
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 5 cut(s) 139, 181, 209, 236, 377
MseI TTAA 2 cut(s) 135, 294
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 110
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 11, 73, 260, 384
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 388
NdeII GATC 2 cut(s) 321, 429
NlaIII CATG 4 cut(s) 226, 274, 392, 404
NlaIV GGNNCC 3 cut(s) 121, 178, 424
NspI RCATGY 1 cut(s) 404
PctI GAATGC 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 294
Psp1406I AACGTT 1 cut(s) 153
PspN4I GGNNCC 3 cut(s) 121, 178, 424
PspPI GGNCC 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 135, 294
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 2 cut(s) 321, 429
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 1 cut(s) 375
SetI ASST 7 cut(s) 46, 106, 141, 156, 171, 352, 428
SfaNI GCATC 2 cut(s) 365, 477
Sse9I AATT 1 cut(s) 488
SsiI CCGC 1 cut(s) 433
SspI AATATT 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 133, 258
TaiI ACGT 2 cut(s) 106, 156
TaqI TCGA 2 cut(s) 106, 186
TasI AATT 1 cut(s) 488
Tru1I TTAA 2 cut(s) 135, 294
Tru9I TTAA 2 cut(s) 135, 294
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 300
TspRI CASTG 1 cut(s) 136
Tth111I GACNNNGTC 1 cut(s) 102
VspI ATTAAT 1 cut(s) 294
XceI RCATGY 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.