Rw5G045280

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001631
Physical Location & Seq
Reverse (-)
79180024 .. 79182145
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045280.1

Sequence Viewer

Length: 741 bp
ATGGCGCTGAGCTCTCTCCGCCTTCACCGAACACTAAGCAGCCTCTCCACCCTCCATACTGTTCTTCCTATCCTCTACTCCAGTCCCCCAACACCTATATCGTCTACTCAGTCCTGGACCTTTATCCAATCTCCGCCTTTTGGCTTTTTGTATGGCAGAAAGGGTGGGGTTACAAGCAGCGGCTCCTTGTCATGGATTTTCGGCAAGACCCAAAGCCTCGGCCTGAGGAGATGGGCAAATATGGCTTTCTTAGCAACTGCTGAAGTTTGTGATACAAATACAGCTCTTTTGGGAAGTGGAGACCTGCGTGTCCTGCCACCAATATTTCAGATGTATGGACAAAGTCGAGCATTCTCAGGCCCCATTTCCACTGTTAAGGTTTTTGAGGATAACGTTTTAGTTAGAGAGCTTCTCGGAACCAGAGGCGAGGGAAGAGTTTTAGTTATTGATGGGGGAGGAAGCATGAGATGTGCTCTAGTTGGAGGGAACTTGGGACAGTTGGCTCAAAACATGGGATGGGCAGGGATTGTGATAAATGGCTGCATTAGAGATGTAGATGAGATCAATGGATGTGATATTGGGGTGAGAGCTTTGGCATCTCATCCCATGAAGTCCAACAAACGAGGCCATGGCGATAAACATGTTCCGATCAACATTGCAGGAACCTTGATCCGCGAAGGGGAATGGTTGTATGCTGACAGTGATGGCATCCTTATCTCAACATCTGAACTGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

246

Amino Acids

26.33

Weight (kDa)

8.76

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 86 - 239 3.3e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 675
AciI CCGC 4 cut(s) 19, 134, 180, 673
AclI AACGTT 1 cut(s) 393
AclWI GGATC 1 cut(s) 664
AcuI CTGAAG 1 cut(s) 282
AfiI CCNNNNNNNGG 3 cut(s) 140, 192, 679
AflIII ACRYGT 1 cut(s) 640
AjnI CCWGG 1 cut(s) 113
AluBI AGCT 4 cut(s) 12, 284, 409, 590
AluI AGCT 4 cut(s) 12, 284, 409, 590
Alw21I GWGCWC 2 cut(s) 14, 475
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 664
AoxI GGCC 3 cut(s) 220, 358, 625
ApeKI GCWGC 3 cut(s) 39, 177, 540
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 117, 359
AsuHPI GGTGA 2 cut(s) 17, 595
AvaII GGWCC 1 cut(s) 117
AxyI CCTNAGG 1 cut(s) 224
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 2 cut(s) 14, 475
BbvI GCAGC 3 cut(s) 51, 189, 527
BccI CCATC 4 cut(s) 225, 443, 510, 698
BciT130I CCWGG 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 312
BisI GCNGC 4 cut(s) 40, 178, 181, 541
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 4 cut(s) 41, 179, 182, 542
Bme1390I CCNGG 1 cut(s) 115
Bme18I GGWCC 1 cut(s) 117
BmgT120I GGNCC 2 cut(s) 117, 359
BmiI GGNNCC 4 cut(s) 184, 361, 418, 664
BmrFI CCNGG 1 cut(s) 115
BmsI GCATC 2 cut(s) 605, 717
BplI GAGNNNNNCTC 4 cut(s) 396, 428, 457, 489
BpmI CTGGAG 1 cut(s) 64
Bpu1102I GCTNAGC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 217, 628
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 192, 679
Bse1I ACTGG 1 cut(s) 81
Bse21I CCTNAGG 1 cut(s) 224
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 2 cut(s) 217, 628
BseGI GGATG 4 cut(s) 521, 575, 601, 708
BseLI CCNNNNNNNGG 3 cut(s) 140, 192, 679
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 3 cut(s) 122, 215, 369
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 241
BseXI GCAGC 3 cut(s) 51, 189, 527
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 3 cut(s) 222, 360, 627
BsiHKAI GWGCWC 2 cut(s) 14, 475
BslFI GGGAC 2 cut(s) 69, 507
BslI CCNNNNNNNGG 3 cut(s) 140, 192, 679
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 2 cut(s) 69, 507
BsmI GAATGC 1 cut(s) 350
BsnI GGCC 3 cut(s) 222, 360, 627
Bso31I GGTCTC 1 cut(s) 294
Bsp1286I GDGCHC 2 cut(s) 14, 475
Bsp143I GATC 3 cut(s) 561, 648, 669
Bsp1720I GCTNAGC 1 cut(s) 8
Bsp19I CCATGG 1 cut(s) 628
BspACI CCGC 4 cut(s) 19, 134, 180, 673
BspANI GGCC 3 cut(s) 222, 360, 627
BspCNI CTCAG 3 cut(s) 121, 216, 368
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 4 cut(s) 184, 361, 418, 664
BspMI ACCTGC 1 cut(s) 312
BspPI GGATC 1 cut(s) 664
BspTNI GGTCTC 1 cut(s) 294
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 217, 628
BssMI GATC 3 cut(s) 561, 648, 669
BssT1I CCWWGG 1 cut(s) 628
Bst2UI CCWGG 1 cut(s) 115
Bst4CI ACNGT 5 cut(s) 61, 373, 498, 701, 732
Bst6I CTCTTC 1 cut(s) 427
BstDEI CTNAG 6 cut(s) 8, 35, 108, 224, 250, 355
BstDSI CCRYGG 1 cut(s) 628
BstF5I GGATG 4 cut(s) 521, 575, 601, 708
BstFNI CGCG 1 cut(s) 675
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 564, 651, 672
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 3 cut(s) 561, 648, 669
BstMWI GCNNNNNNNGC 4 cut(s) 18, 242, 251, 313
BstNI CCWGG 1 cut(s) 115
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 113
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 51, 189, 527
Bsu36I CCTNAGG 1 cut(s) 224
BsuRI GGCC 3 cut(s) 222, 360, 627
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 4 cut(s) 521, 575, 601, 708
BtsIMutI CAGTG 2 cut(s) 369, 706
BveI ACCTGC 1 cut(s) 312
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 117, 359
CviAII CATG 6 cut(s) 192, 463, 511, 607, 629, 641
DdeI CTNAG 6 cut(s) 8, 35, 108, 224, 250, 355
DpnI GATC 3 cut(s) 563, 650, 671
DpnII GATC 3 cut(s) 561, 648, 669
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 427
EarI CTCTTC 1 cut(s) 427
EciI GGCGGA 2 cut(s) 8, 123
Ecl136II GAGCTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 628
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 628
FaeI CATG 6 cut(s) 195, 466, 514, 610, 632, 644
FaqI GGGAC 2 cut(s) 69, 507
FatI CATG 6 cut(s) 191, 462, 510, 606, 628, 640
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 4 cut(s) 40, 178, 181, 541
FokI GGATG 4 cut(s) 528, 582, 588, 695
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 4 cut(s) 40, 178, 181, 541
FspBI CTAG 1 cut(s) 476
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 40, 178, 181, 541
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 222, 360, 627
HhaI GCGC 1 cut(s) 7
Hin1II CATG 6 cut(s) 195, 466, 514, 610, 632, 644
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HphI GGTGA 2 cut(s) 17, 595
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 4 cut(s) 330, 416, 648, 727
Hpy8I GTNNAC 1 cut(s) 105
HpyAV CCTTC 2 cut(s) 32, 671
HpyCH4III ACNGT 5 cut(s) 61, 373, 498, 701, 732
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 2 cut(s) 543, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 242, 251, 313
HpyF3I CTNAG 6 cut(s) 8, 35, 108, 224, 250, 355
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 6 cut(s) 195, 466, 514, 610, 632, 644
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 3 cut(s) 561, 648, 669
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 3 cut(s) 51, 189, 527
LweI GCATC 2 cut(s) 605, 717
MaeI CTAG 1 cut(s) 476
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 169
MalI GATC 3 cut(s) 563, 650, 671
MboI GATC 3 cut(s) 561, 648, 669
MboII GAAGA 2 cut(s) 56, 444
MhlI GDGCHC 2 cut(s) 14, 475
MmeI TCCRAC 2 cut(s) 460, 639
MseI TTAA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 1 cut(s) 115
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 4 cut(s) 18, 242, 251, 313
NcoI CCATGG 1 cut(s) 628
NdeII GATC 3 cut(s) 561, 648, 669
NlaIII CATG 6 cut(s) 195, 466, 514, 610, 632, 644
NlaIV GGNNCC 4 cut(s) 184, 361, 418, 664
NmeAIII GCCGAG 1 cut(s) 198
NspI RCATGY 1 cut(s) 644
PciI ACATGT 1 cut(s) 640
PctI GAATGC 1 cut(s) 350
PflFI GACNNNGTC 1 cut(s) 342
PfoI TCCNGGA 1 cut(s) 113
PkrI GCNGC 4 cut(s) 41, 179, 182, 542
PscI ACATGT 1 cut(s) 640
Psp124BI GAGCTC 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 4 cut(s) 184, 361, 418, 664
PspPI GGNCC 2 cut(s) 117, 359
PsyI GACNNNGTC 1 cut(s) 342
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 4 cut(s) 40, 178, 181, 541
Sau3AI GATC 3 cut(s) 561, 648, 669
Sau96I GGNCC 2 cut(s) 117, 359
ScrFI CCNGG 1 cut(s) 115
SduI GDGCHC 2 cut(s) 14, 475
SfaNI GCATC 2 cut(s) 605, 717
SinI GGWCC 1 cut(s) 117
SsiI CCGC 4 cut(s) 19, 134, 180, 673
SspI AATATT 1 cut(s) 324
SspMI CTAG 1 cut(s) 476
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 113
StyI CCWWGG 1 cut(s) 628
TaaI ACNGT 5 cut(s) 61, 373, 498, 701, 732
TaiI ACGT 1 cut(s) 396
TaqI TCGA 1 cut(s) 346
TauI GCSGC 1 cut(s) 183
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 376, 706
TseI GCWGC 3 cut(s) 39, 177, 540
TspDTI ATGAA 1 cut(s) 623
TspRI CASTG 2 cut(s) 376, 706
Tth111I GACNNNGTC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 117
XceI RCATGY 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 104
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.