RLG00000036312

Aldolase/RraA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001631
Physical Location & Seq
Forward (+)
78842419 .. 78845681
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036312

Sequence Viewer

Length: 732 bp
ATGCATGTAAGGGTGATCTTCCGACGAGGAGATAGCGCTATTTTCCTGTGGGTTGGTGGAATCGGTGGCTATGGCTTACGATTGGAATCCGAGGACAAGGCTAATCGGTGGAAGGCCGGTGTTGGTTCCGGTGGTGCAGGGCCCACGCTGGTGGTAGGTCGAGTGCGTGCACGCCGTGCTGCAGATGCTGATGAAGGTTCTAATGGTGATGGCTCTGTCGGCTCTGGAGCCGAGTACAGGAGAAGTCTGGCAACCACAAACGTTTGTGACGCAAATCCCGGACTGTTGGAGAGCGGAGACTTGCGTCTTTTGGGGCCAATCTTTCAAAATTTTGGAAAATGGAGCGCGTTTTCGGATCAGGTGGTGACCCTCAAGTTGTTTGAGGACAACACTTCGGTGATCGAGGCCCTGTCGAGAAAAGGTGAGGGGAGGGTGTTGGTTGTGGACGGCGGAGGAAGCATGAGTCGTGCCGTCACTGGAGGGATGCTGGGCAAGTGTGCCGAAATTATGGGGTGGGCTGGGATTGTGGTGAATGGTTGCGTCAGAGATGTGGATGAGATCAATGAATGTGAGATCGGGGTGATAGCTTTGGGGACTTGCCCTGTGAGACCGAAAAGAAGTAAAGATGGGAGTGGCCACAAGCATGTTGATGTCCATATTGAAGGAAAATGGATTCATGATGGGGAATGGTTGTACGCAGATATTGATGGCATCCTCGTCTCCAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

244

Amino Acids

25.75

Weight (kDa)

6.23

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 85 - 240 4.3e-38 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 294
AccII CGCG 1 cut(s) 347
AciI CCGC 2 cut(s) 294, 450
AclI AACGTT 1 cut(s) 261
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 1 cut(s) 634
AcsI RAATTY 1 cut(s) 328
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 2 cut(s) 236, 695
AfeI AGCGCT 1 cut(s) 37
AfiI CCNNNNNNNGG 1 cut(s) 237
AgsI TTSAA 2 cut(s) 326, 662
AleI CACNNNNGTG 2 cut(s) 149, 395
AluBI AGCT 1 cut(s) 587
AluI AGCT 1 cut(s) 587
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 3 cut(s) 291, 601, 724
Alw44I GTGCAC 1 cut(s) 168
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 37
AoxI GGCC 5 cut(s) 114, 140, 314, 405, 634
ApaI GGGCCC 1 cut(s) 144
ApaLI GTGCAC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 1 cut(s) 328
AspLEI GCGC 2 cut(s) 38, 347
AspS9I GGNCC 4 cut(s) 140, 141, 314, 406
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 7 cut(s) 25, 218, 376, 409, 434, 541, 592
BaeGI GKGCMC 2 cut(s) 144, 172
BalI TGGCCA 1 cut(s) 636
BanII GRGCYC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 166
BccI CCATC 4 cut(s) 203, 620, 674, 701
BceAI ACGGC 3 cut(s) 159, 455, 463
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 291, 601, 724
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 279
BmgT120I GGNCC 4 cut(s) 140, 141, 314, 406
BmiI GGNNCC 4 cut(s) 127, 142, 229, 315
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 3 cut(s) 175, 474, 720
BoxI GACNNNNGTC 1 cut(s) 303
BpmI CTGGAG 2 cut(s) 246, 498
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 85
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 128
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 481
Bse8I GATNNNNATC 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 3 cut(s) 489, 559, 711
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 1 cut(s) 42
BseSI GKGCMC 2 cut(s) 144, 172
BseXI GCAGC 1 cut(s) 166
BseYI CCCAGC 2 cut(s) 487, 518
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 5 cut(s) 116, 142, 316, 407, 636
BsiHKAI GWGCWC 1 cut(s) 172
BsiSI CCGG 3 cut(s) 117, 129, 279
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 1 cut(s) 237
BsmAI GTCTC 3 cut(s) 291, 601, 724
BsmBI CGTCTC 1 cut(s) 724
BsmFI GGGAC 1 cut(s) 607
BsnI GGCC 5 cut(s) 116, 142, 316, 407, 636
Bso31I GGTCTC 1 cut(s) 601
Bsp120I GGGCCC 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 144, 172
Bsp143I GATC 5 cut(s) 15, 355, 399, 558, 573
BspACI CCGC 2 cut(s) 294, 450
BspANI GGCC 5 cut(s) 116, 142, 316, 407, 636
BspFNI CGCG 1 cut(s) 347
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 4 cut(s) 127, 142, 229, 315
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 601
BsrBI CCGCTC 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 481
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 5 cut(s) 15, 355, 399, 558, 573
Bst4CI ACNGT 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 2 cut(s) 168, 172
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 3 cut(s) 489, 559, 711
BstFNI CGCG 1 cut(s) 347
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 347
BstKTI GATC 5 cut(s) 18, 358, 402, 561, 576
BstMAI GTCTC 3 cut(s) 291, 601, 724
BstMBI GATC 5 cut(s) 15, 355, 399, 558, 573
BstMWI GCNNNNNNNGC 4 cut(s) 176, 185, 219, 456
BstNSI RCATGY 2 cut(s) 8, 647
BstPAI GACNNNNGTC 1 cut(s) 303
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 277
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 2 cut(s) 144, 172
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 166
BstXI CCANNNNNNTGG 1 cut(s) 151
BsuRI GGCC 5 cut(s) 116, 142, 316, 407, 636
BtsCI GGATG 3 cut(s) 489, 559, 711
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 2 cut(s) 168, 172
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 676
CfoI GCGC 2 cut(s) 38, 347
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 4 cut(s) 140, 141, 314, 406
CseI GACGC 3 cut(s) 278, 293, 529
Csp6I GTAC 2 cut(s) 235, 694
CviAII CATG 4 cut(s) 5, 460, 644, 677
CviQI GTAC 2 cut(s) 235, 694
DpnI GATC 5 cut(s) 17, 357, 401, 560, 575
DpnII GATC 5 cut(s) 15, 355, 399, 558, 573
DraIII CACNNNGTG 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 634
EciI GGCGGA 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 601
Eco47III AGCGCT 1 cut(s) 37
Eco91I GGTNACC 1 cut(s) 364
EcoO109I RGGNCCY 2 cut(s) 140, 406
EcoO65I GGTNACC 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 144
Esp3I CGTCTC 1 cut(s) 724
FaeI CATG 4 cut(s) 8, 463, 647, 680
FaiI YATR 7 cut(s) 6, 72, 461, 509, 645, 657, 678
FaqI GGGAC 1 cut(s) 607
FatI CATG 4 cut(s) 4, 459, 643, 676
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 3 cut(s) 496, 566, 698
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 180
GlaI GCGC 2 cut(s) 37, 346
GluI GCNGC 1 cut(s) 180
GsaI CCCAGC 2 cut(s) 491, 522
GsuI CTGGAG 2 cut(s) 246, 498
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 5 cut(s) 116, 142, 316, 407, 636
HapII CCGG 3 cut(s) 117, 129, 279
HgaI GACGC 3 cut(s) 278, 293, 529
HhaI GCGC 2 cut(s) 38, 347
Hin1II CATG 4 cut(s) 8, 463, 647, 680
Hin6I GCGC 2 cut(s) 36, 345
HinP1I GCGC 2 cut(s) 36, 345
HinfI GANTC 4 cut(s) 60, 86, 463, 673
HpaII CCGG 3 cut(s) 117, 129, 279
HphI GGTGA 7 cut(s) 25, 218, 376, 409, 434, 541, 592
Hpy166II GTNNAC 2 cut(s) 170, 445
Hpy188I TCNGA 4 cut(s) 23, 91, 355, 545
Hpy188III TCNNGA 3 cut(s) 225, 414, 677
Hpy8I GTNNAC 2 cut(s) 170, 445
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 3 cut(s) 106, 188, 656
HpyCH4III ACNGT 1 cut(s) 285
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 4, 137, 170, 182
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 185, 219, 456
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 4 cut(s) 8, 463, 647, 680
HspAI GCGC 2 cut(s) 36, 345
Kzo9I GATC 5 cut(s) 15, 355, 399, 558, 573
LmnI GCTCC 2 cut(s) 227, 342
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 3 cut(s) 175, 474, 720
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 266, 364, 472
MalI GATC 5 cut(s) 17, 357, 401, 560, 575
MbiI CCGCTC 1 cut(s) 294
MboI GATC 5 cut(s) 15, 355, 399, 558, 573
MboII GAAGA 1 cut(s) 10
MhlI GDGCHC 2 cut(s) 144, 172
MlsI TGGCCA 1 cut(s) 636
MluCI AATT 2 cut(s) 328, 504
MluNI TGGCCA 1 cut(s) 636
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 2 cut(s) 46, 267
Mox20I TGGCCA 1 cut(s) 636
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 636
MseI TTAA 1 cut(s) 730
MslI CAYNNNNRTG 4 cut(s) 149, 395, 642, 648
Msp20I TGGCCA 1 cut(s) 636
MspI CCGG 3 cut(s) 117, 129, 279
MspR9I CCNGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 176, 185, 219, 456
NciI CCSGG 1 cut(s) 279
NdeII GATC 5 cut(s) 15, 355, 399, 558, 573
NlaIII CATG 4 cut(s) 8, 463, 647, 680
NlaIV GGNNCC 4 cut(s) 127, 142, 229, 315
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 3 cut(s) 266, 364, 472
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 647
OliI CACNNNNGTG 2 cut(s) 149, 395
PagI TCATGA 1 cut(s) 676
PfeI GAWTC 3 cut(s) 60, 86, 673
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
PshAI GACNNNNGTC 1 cut(s) 303
Psp1406I AACGTT 1 cut(s) 261
PspEI GGTNACC 1 cut(s) 364
PspFI CCCAGC 2 cut(s) 487, 518
PspN4I GGNNCC 4 cut(s) 127, 142, 229, 315
PspOMI GGGCCC 1 cut(s) 140
PspPI GGNCC 4 cut(s) 140, 141, 314, 406
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 236, 695
RsaNI GTAC 2 cut(s) 235, 694
RseI CAYNNNNRTG 4 cut(s) 149, 395, 642, 648
SaqAI TTAA 1 cut(s) 730
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 5 cut(s) 15, 355, 399, 558, 573
Sau96I GGNCC 4 cut(s) 140, 141, 314, 406
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 144, 172
SetI ASST 6 cut(s) 160, 199, 264, 363, 424, 589
SfaNI GCATC 3 cut(s) 175, 474, 720
SfcI CTRYAG 1 cut(s) 180
SmiMI CAYNNNNRTG 4 cut(s) 149, 395, 642, 648
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 2 cut(s) 328, 504
SsiI CCGC 2 cut(s) 294, 450
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 285
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 160, 402, 413
TaqII GACCGA 1 cut(s) 625
TasI AATT 2 cut(s) 328, 504
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 3 cut(s) 60, 86, 673
Tru1I TTAA 1 cut(s) 730
Tru9I TTAA 1 cut(s) 730
TscAI CASTG 1 cut(s) 481
TseFI GTSAC 3 cut(s) 266, 364, 472
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 3 cut(s) 266, 364, 472
TspDTI ATGAA 3 cut(s) 207, 579, 665
TspRI CASTG 1 cut(s) 481
VneI GTGCAC 1 cut(s) 168
XapI RAATTY 1 cut(s) 328
XceI RCATGY 2 cut(s) 8, 647
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.