FvH4_4g04510

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001988
Physical Location & Seq
Forward (+)
3853050 .. 3855264
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04510.t1

Sequence Viewer

Length: 420 bp
ATGCCCTCCCAGACTCCGATTCAGATTCACTCCTCCTCCGACTCCAAGACCTCCTATGCCGTCATCTTCGACACCGGCAGCTCCGGCAGCCGCGTCCACGTCTTCTGCTTCGACCACAACCTCCTCCCCATCGGAAAAGATCTCGAGCTCTTCCTCCAGACCAAGCCCGGTCTCAGCTCCTACGCCTCCAACCCTCGTAGCGCCACCTTGTCGCTCTCAGATCTCTTCCACGAGGCCGAAGCCGCCGTTCCTAAGGATCTATGTCCCTTCACCCTCGTCTGCGTCGGAGCAACCGCCAGCCTCCGAGCGTTAGGTGTTGATGCATCTAATCGCATTCTCCAGGCCGTTAGGGATTTGCTCAAGCAGTCAAGCTCCCTCAGCTCCCAACCTGATGCTGTTACTGTGTGCATGTTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 map00230 map00240
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

140

Amino Acids

14.86

Weight (kDa)

5.73

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDA1_CD39 PF01150 13 - 135 2.1e-28 GDA1/CD39 (nucleoside phosphatase) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 93
AciI CCGC 3 cut(s) 91, 243, 294
AclWI GGATC 1 cut(s) 264
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 5 cut(s) 81, 148, 177, 372, 381
AluI AGCT 5 cut(s) 81, 148, 177, 372, 381
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 264
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 234, 342
ApeKI GCWGC 2 cut(s) 78, 87
AspLEI GCGC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 143
AxyI CCTNAGG 1 cut(s) 252
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 377
BbvI GCAGC 2 cut(s) 90, 99
BccI CCATC 1 cut(s) 137
BceAI ACGGC 3 cut(s) 44, 230, 329
BciT130I CCWGG 1 cut(s) 341
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 176
BfoI RGCGCY 1 cut(s) 204
BglII AGATCT 2 cut(s) 139, 220
BisI GCNGC 4 cut(s) 79, 88, 91, 243
BlsI GCNGC 4 cut(s) 80, 89, 92, 244
Bme1390I CCNGG 2 cut(s) 168, 341
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 100
BmrFI CCNGG 2 cut(s) 168, 341
BmsI GCATC 3 cut(s) 310, 332, 382
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 2 cut(s) 140, 323
Bpu10I CCTNAGC 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bse118I RCCGGY 1 cut(s) 74
Bse21I CCTNAGG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 341
BseMII CTCAG 3 cut(s) 187, 231, 391
BseRI GAGGAG 3 cut(s) 22, 25, 113
BseXI GCAGC 2 cut(s) 90, 99
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 236, 344
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 3 cut(s) 75, 84, 168
BslFI GGGAC 1 cut(s) 249
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 249
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 2 cut(s) 236, 344
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 3 cut(s) 139, 220, 256
BspACI CCGC 3 cut(s) 91, 243, 294
BspANI GGCC 2 cut(s) 236, 344
BspCNI CTCAG 3 cut(s) 186, 230, 390
BspFNI CGCG 1 cut(s) 93
BspPI GGATC 1 cut(s) 264
BspQI GCTCTTC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssMI GATC 3 cut(s) 139, 220, 256
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 2 cut(s) 155, 230
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 4 cut(s) 173, 217, 252, 377
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 3 cut(s) 142, 223, 259
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 3 cut(s) 139, 220, 256
BstMWI GCNNNNNNNGC 4 cut(s) 84, 87, 242, 378
BstNI CCWGG 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 166, 339
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 90, 99
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 3 cut(s) 139, 220, 256
BstYI RGATCY 3 cut(s) 139, 220, 256
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 2 cut(s) 236, 344
BtrI CACGTC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 298
CfoI GCGC 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 74
CseI GACGC 2 cut(s) 82, 271
CviAII CATG 1 cut(s) 409
DdeI CTNAG 4 cut(s) 173, 217, 252, 377
DpnI GATC 3 cut(s) 141, 222, 258
DpnII GATC 3 cut(s) 139, 220, 256
Eam1104I CTCTTC 2 cut(s) 155, 230
EarI CTCTTC 2 cut(s) 155, 230
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 148
Eco81I CCTNAGG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 1 cut(s) 412
FaiI YATR 3 cut(s) 57, 262, 410
FaqI GGGAC 1 cut(s) 249
FatI CATG 1 cut(s) 408
Fnu4HI GCNGC 4 cut(s) 79, 88, 91, 243
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 79, 88, 91, 243
GlaI GCGC 1 cut(s) 202
GluI GCNGC 4 cut(s) 79, 88, 91, 243
GsuI CTGGAG 2 cut(s) 140, 323
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 2 cut(s) 236, 344
HapII CCGG 3 cut(s) 75, 84, 168
HgaI GACGC 2 cut(s) 82, 271
HhaI GCGC 1 cut(s) 203
Hin1II CATG 1 cut(s) 412
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 4 cut(s) 13, 19, 25, 41
HpaII CCGG 3 cut(s) 75, 84, 168
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 7 cut(s) 18, 24, 40, 134, 220, 287, 305
Hpy188III TCNNGA 2 cut(s) 143, 157
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 2 cut(s) 323, 408
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 87, 242, 378
HpyF3I CTNAG 4 cut(s) 173, 217, 252, 377
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 1 cut(s) 412
HspAI GCGC 1 cut(s) 201
Kzo9I GATC 3 cut(s) 139, 220, 256
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 5 cut(s) 86, 182, 287, 377, 386
LpnPI CCDG 9 cut(s) 23, 88, 97, 170, 181, 310, 326, 353, 402
Lsp1109I GCAGC 2 cut(s) 90, 99
LweI GCATC 3 cut(s) 310, 332, 382
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 141, 222, 258
MboI GATC 3 cut(s) 139, 220, 256
MboII GAAGA 4 cut(s) 58, 94, 142, 217
MflI RGATCY 3 cut(s) 139, 220, 256
MhlI GDGCHC 1 cut(s) 150
MlyI GAGTC 2 cut(s) 7, 35
MmeI TCCRAC 3 cut(s) 63, 213, 265
Mph1103I ATGCAT 1 cut(s) 325
MspI CCGG 3 cut(s) 75, 84, 168
MspR9I CCNGG 2 cut(s) 168, 341
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 84, 87, 242, 378
NciI CCSGG 1 cut(s) 168
NdeII GATC 3 cut(s) 139, 220, 256
NlaIII CATG 1 cut(s) 412
NsiI ATGCAT 1 cut(s) 325
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 155
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 19, 25
PkrI GCNGC 4 cut(s) 80, 89, 92, 244
PleI GAGTC 2 cut(s) 7, 35
PpsI GAGTC 2 cut(s) 7, 35
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PsuI RGATCY 3 cut(s) 139, 220, 256
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 155
SatI GCNGC 4 cut(s) 79, 88, 91, 243
Sau3AI GATC 3 cut(s) 139, 220, 256
SchI GAGTC 2 cut(s) 7, 35
ScrFI CCNGG 2 cut(s) 168, 341
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 3 cut(s) 310, 332, 382
Sfr274I CTCGAG 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 143, 359
SmoI CTYRAG 2 cut(s) 143, 359
SsiI CCGC 3 cut(s) 91, 243, 294
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 166, 339
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 69, 111, 144
TauI GCSGC 2 cut(s) 93, 245
TfiI GAWTC 2 cut(s) 19, 25
TseI GCWGC 2 cut(s) 78, 87
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.