MD12G1000500.v1.1

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001988
Physical Location & Seq
Forward (+)
153211 .. 156223
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1000500.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGCTCAAGCGTCCCGGCAGGGGAATTGGAATGGGGAATCCCCAGTACGACTCCCTCTCCGACAAAATGTACAGATACAGGAGCGTCATCCTCGTCATCTCAATTCCTCTCCTCCTCCTTACCATGGTCCTCTACGTCATGCCCCCCGGGGGTAATTTGAGCAACCAGCTCCCCCACAAGCTGCCTTCCAAGACCTCCTACGCCGTGATCTTCGACGCCGGCAGCTCCGGCAGCCGCGTCCACGTCTTCTGCTTCGACAACAACCTCCAGCTCCTTCCCATCGGCAAAGATCTCGAGCTCTTTCTTCAGGTCAAGCCTGGCCTGAGCGCTTATGCCAAGGACCATCGCTCTGCTGCCGAGTCCCTCACCGGTCTTCTCGATAAGGCTGAGAGCGCAGTTCCCAAGGAGCTACGTTCCTTCACTCCCGTCCGCGTTGGGGCTACGGCTGGCCTGAGGGCTTTGAAGGGCGATGCTTCTGACCGCATTCTCCAGGCCGTTAGGGATCTGCTCAAACAAAGAAGCAGCCTCAGCTCCCGCCCTGATGCCGTTACTGTCATTGATGGAACTCAAGAGGGTTCTTTCCAGTGGGTGACAATAAACTATCTACTAGGAAATTTGGGGAAGAAATATTCAGATACTGTAGGGGTGGTTGACCTTGGGGGTGGATCTGTTCAGATGGCTTATGCTATCTCAGAGGCAGATGCTGCAAAAGCACCCAAGTTATCAGATGGAGAGGATGTATATGTAAGGGAAATGAATCTTAAGGGAGCTAAATATAACCTTTATGTTCACAGCTACTTGCATTACGGGTTACTAGCAGCTCGAGCAGAGATTTTAAAGGTTTCTGAAGATTCTGACAACCCCTGCATCTTAGCTGGATATTACGGTTCTTACACATATGGAGGACAATCTTATAAAGCATCAGCTTCCTCATCTGGATCAAGTGTTGAGGAGTGCAGGAGGGTAGTTTCAAGGGCACTCAAAGTAAACGAAGCAACATGTTCACACATGAAGTGCACATTTGGTGGGGTATGGAATGGTGGAGGAGGGGGTGGACAAAGGAACCTATTTGTGGCTTCATTTTTCTTTGACAGAGCTGCTGAGGTATTTCACCATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 map00230 map00240
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

374

Amino Acids

40.33

Weight (kDa)

8.99

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDA1_CD39 PF01150 62 - 364 1.1e-85 GDA1/CD39 (nucleoside phosphatase) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AccII CGCG 2 cut(s) 237, 432
AciI CCGC 4 cut(s) 235, 430, 481, 535
AclWI GGATC 3 cut(s) 510, 673, 946
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 2 cut(s) 290, 867
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 47, 71
AfeI AGCGCT 1 cut(s) 328
AfiI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 436, 1072
AflII CTTAAG 1 cut(s) 761
AflIII ACRYGT 1 cut(s) 998
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 2 cut(s) 463, 972
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 2 cut(s) 316, 489
Alw21I GWGCWC 2 cut(s) 300, 1019
Alw44I GTGCAC 1 cut(s) 1015
AlwI GGATC 3 cut(s) 510, 673, 946
AlwNI CAGNNNCTG 2 cut(s) 638, 704
Ama87I CYCGRG 3 cut(s) 146, 293, 822
Aor51HI AGCGCT 1 cut(s) 328
AoxI GGCC 3 cut(s) 319, 448, 492
ApaLI GTGCAC 1 cut(s) 1015
ApeKI GCWGC 8 cut(s) 181, 222, 231, 353, 522, 704, 818, 1097
ApoI RAATTY 1 cut(s) 613
AsiGI ACCGGT 1 cut(s) 368
AspLEI GCGC 2 cut(s) 329, 395
AspS9I GGNCC 2 cut(s) 127, 340
AsuC2I CCSGG 3 cut(s) 15, 147, 148
AsuHPI GGTGA 3 cut(s) 358, 601, 1103
AvaI CYCGRG 3 cut(s) 146, 293, 822
AvaII GGWCC 2 cut(s) 127, 340
AxyI CCTNAGG 1 cut(s) 452
BaeGI GKGCMC 2 cut(s) 979, 1019
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 238, 365
Bbv12I GWGCWC 2 cut(s) 300, 1019
BbvCI CCTCAGC 2 cut(s) 527, 1101
BbvI GCAGC 8 cut(s) 168, 234, 243, 340, 534, 691, 830, 1084
BccI CCATC 5 cut(s) 287, 351, 554, 670, 722
BceAI ACGGC 4 cut(s) 188, 459, 479, 530
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 2 cut(s) 318, 491
BcnI CCSGG 3 cut(s) 15, 147, 148
BfaI CTAG 2 cut(s) 608, 815
BfmI CTRYAG 1 cut(s) 639
BfoI RGCGCY 1 cut(s) 330
BfrI CTTAAG 1 cut(s) 761
BglII AGATCT 1 cut(s) 289
BisI GCNGC 9 cut(s) 182, 223, 232, 235, 354, 523, 705, 819, 1098
BlsI GCNGC 9 cut(s) 183, 224, 233, 236, 355, 524, 706, 820, 1099
Bme1390I CCNGG 5 cut(s) 15, 147, 148, 318, 491
Bme18I GGWCC 2 cut(s) 127, 340
BmeT110I CYCGRG 3 cut(s) 146, 293, 822
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 127, 340
BmiI GGNNCC 1 cut(s) 1064
BmrFI CCNGG 5 cut(s) 15, 147, 148, 318, 491
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 5 cut(s) 460, 532, 691, 876, 929
BmuI ACTGGG 1 cut(s) 37
BpiI GAAGAC 2 cut(s) 238, 365
BpmI CTGGAG 2 cut(s) 251, 473
Bpu10I CCTNAGC 3 cut(s) 323, 527, 1101
BpuEI CTTGAG 1 cut(s) 552
BpuMI CCSGG 3 cut(s) 15, 147, 148
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 7 cut(s) 123, 145, 146, 147, 336, 402, 655
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 723, 753
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 124, 149, 436, 1072
Bse118I RCCGGY 2 cut(s) 218, 368
Bse1I ACTGG 2 cut(s) 43, 583
Bse21I CCTNAGG 1 cut(s) 452
BseBI CCWGG 2 cut(s) 318, 491
BseDI CCNNGG 7 cut(s) 123, 145, 146, 147, 336, 402, 655
BseGI GGATG 3 cut(s) 87, 742, 1114
BseLI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 436, 1072
BseMII CTCAG 6 cut(s) 314, 378, 443, 541, 705, 1092
BseNI ACTGG 2 cut(s) 43, 583
BseRI GAGGAG 4 cut(s) 101, 104, 965, 1059
BseSI GKGCMC 2 cut(s) 979, 1019
BseXI GCAGC 8 cut(s) 168, 234, 243, 340, 534, 691, 830, 1084
BsgI GTGCAG 1 cut(s) 976
Bsh1236I CGCG 2 cut(s) 237, 432
BshFI GGCC 3 cut(s) 321, 450, 494
BshTI ACCGGT 1 cut(s) 368
BsiHKAI GWGCWC 2 cut(s) 300, 1019
BsiHKCI CYCGRG 3 cut(s) 146, 293, 822
BsiSI CCGG 5 cut(s) 15, 147, 219, 228, 369
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 5 cut(s) 20, 124, 149, 436, 1072
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 3 cut(s) 321, 450, 494
BsoBI CYCGRG 3 cut(s) 146, 293, 822
Bsp1286I GDGCHC 3 cut(s) 300, 979, 1019
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 5 cut(s) 207, 289, 502, 665, 938
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 4 cut(s) 235, 430, 481, 535
BspANI GGCC 3 cut(s) 321, 450, 494
BspCNI CTCAG 6 cut(s) 315, 379, 444, 540, 704, 1093
BspFNI CGCG 2 cut(s) 237, 432
BspLI GGNNCC 1 cut(s) 1064
BspPI GGATC 3 cut(s) 510, 673, 946
BspTI CTTAAG 1 cut(s) 761
BsrFI RCCGGY 2 cut(s) 218, 368
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 2 cut(s) 43, 583
BssAI RCCGGY 2 cut(s) 218, 368
BssECI CCNNGG 7 cut(s) 123, 145, 146, 147, 336, 402, 655
BssMI GATC 5 cut(s) 207, 289, 502, 665, 938
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 4 cut(s) 123, 336, 402, 655
Bst2UI CCWGG 2 cut(s) 318, 491
Bst4CI ACNGT 3 cut(s) 553, 640, 887
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 704
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 220, 448
BstDEI CTNAG 7 cut(s) 323, 387, 452, 527, 691, 871, 1101
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 87, 742, 1114
BstFNI CGCG 2 cut(s) 237, 432
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 2 cut(s) 329, 395
BstKTI GATC 5 cut(s) 210, 292, 505, 668, 941
BstMBI GATC 5 cut(s) 207, 289, 502, 665, 938
BstMWI GCNNNNNNNGC 7 cut(s) 228, 231, 392, 528, 704, 710, 824
BstNI CCWGG 2 cut(s) 318, 491
BstNSI RCATGY 1 cut(s) 1002
BstSCI CCNGG 5 cut(s) 13, 145, 146, 316, 489
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 2 cut(s) 979, 1019
BstUI CGCG 2 cut(s) 237, 432
BstV1I GCAGC 8 cut(s) 168, 234, 243, 340, 534, 691, 830, 1084
BstV2I GAAGAC 2 cut(s) 238, 365
BstX2I RGATCY 3 cut(s) 289, 502, 665
BstYI RGATCY 3 cut(s) 289, 502, 665
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 3 cut(s) 321, 450, 494
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 2 cut(s) 329, 483
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 3 cut(s) 87, 742, 1114
BtsIMutI CAGTG 1 cut(s) 590
Cac8I GCNNGC 2 cut(s) 220, 448
CaiI CAGNNNCTG 2 cut(s) 638, 704
CfoI GCGC 2 cut(s) 329, 395
Cfr10I RCCGGY 2 cut(s) 218, 368
Cfr13I GGNCC 2 cut(s) 127, 340
Cfr9I CCCGGG 1 cut(s) 146
CseI GACGC 3 cut(s) 73, 224, 226
Csp6I GTAC 2 cut(s) 46, 70
CspAI ACCGGT 1 cut(s) 368
CviAII CATG 4 cut(s) 124, 139, 999, 1009
CviQI GTAC 2 cut(s) 46, 70
DdeI CTNAG 7 cut(s) 323, 387, 452, 527, 691, 871, 1101
DpnI GATC 5 cut(s) 209, 291, 504, 667, 940
DpnII GATC 5 cut(s) 207, 289, 502, 665, 938
DraI TTTAAA 1 cut(s) 837
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 4 cut(s) 123, 336, 402, 655
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 127, 340
Eco47III AGCGCT 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 290, 867
Eco81I CCTNAGG 1 cut(s) 452
Eco88I CYCGRG 3 cut(s) 146, 293, 822
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 2 cut(s) 316, 489
EcoT14I CCWWGG 4 cut(s) 123, 336, 402, 655
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 4 cut(s) 123, 336, 402, 655
FaeI CATG 4 cut(s) 127, 142, 1002, 1012
FaqI GGGAC 1 cut(s) 346
FatI CATG 4 cut(s) 123, 138, 998, 1008
FauI CCCGC 1 cut(s) 542
FauNDI CATATG 1 cut(s) 898
Fnu4HI GCNGC 9 cut(s) 182, 223, 232, 235, 354, 523, 705, 819, 1098
FokI GGATG 3 cut(s) 74, 749, 1101
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 9 cut(s) 182, 223, 232, 235, 354, 523, 705, 819, 1098
FspBI CTAG 2 cut(s) 608, 815
GlaI GCGC 2 cut(s) 328, 394
GluI GCNGC 9 cut(s) 182, 223, 232, 235, 354, 523, 705, 819, 1098
GsuI CTGGAG 2 cut(s) 251, 473
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 3 cut(s) 321, 450, 494
HapII CCGG 5 cut(s) 15, 147, 219, 228, 369
HgaI GACGC 3 cut(s) 73, 224, 226
HhaI GCGC 2 cut(s) 329, 395
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 4 cut(s) 127, 142, 1002, 1012
Hin6I GCGC 2 cut(s) 327, 393
HinP1I GCGC 2 cut(s) 327, 393
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 5 cut(s) 37, 50, 359, 757, 851
HpaII CCGG 5 cut(s) 15, 147, 219, 228, 369
HphI GGTGA 3 cut(s) 358, 601, 1103
Hpy166II GTNNAC 7 cut(s) 241, 652, 790, 988, 1004, 1017, 1055
Hpy188I TCNGA 8 cut(s) 61, 478, 634, 675, 694, 727, 847, 856
Hpy188III TCNNGA 4 cut(s) 293, 377, 569, 936
Hpy8I GTNNAC 7 cut(s) 241, 652, 790, 988, 1004, 1017, 1055
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 195, 284, 427, 457
HpyCH4III ACNGT 3 cut(s) 553, 640, 887
HpyCH4IV ACGT 3 cut(s) 135, 243, 412
HpyCH4V TGCA 5 cut(s) 707, 802, 867, 957, 1017
HpyF10VI GCNNNNNNNGC 7 cut(s) 228, 231, 392, 528, 704, 710, 824
HpyF3I CTNAG 7 cut(s) 323, 387, 452, 527, 691, 871, 1101
HpySE526I ACGT 3 cut(s) 135, 243, 412
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 4 cut(s) 127, 142, 1002, 1012
HspAI GCGC 2 cut(s) 327, 393
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 5 cut(s) 207, 289, 502, 665, 938
LmnI GCTCC 7 cut(s) 81, 174, 230, 276, 406, 536, 767
Lsp1109I GCAGC 8 cut(s) 168, 234, 243, 340, 534, 691, 830, 1084
LweI GCATC 5 cut(s) 460, 532, 691, 876, 929
MaeI CTAG 2 cut(s) 608, 815
MaeII ACGT 3 cut(s) 135, 243, 412
MaeIII GTNAC 3 cut(s) 547, 589, 810
MalI GATC 5 cut(s) 209, 291, 504, 667, 940
MboI GATC 5 cut(s) 207, 289, 502, 665, 938
MboII GAAGA 6 cut(s) 202, 238, 296, 365, 634, 860
MflI RGATCY 3 cut(s) 289, 502, 665
MhlI GDGCHC 3 cut(s) 300, 979, 1019
MluCI AATT 4 cut(s) 24, 102, 154, 613
MlyI GAGTC 2 cut(s) 44, 368
MmeI TCCRAC 1 cut(s) 84
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 3 cut(s) 762, 836, 1120
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 5 cut(s) 15, 147, 219, 228, 369
MspR9I CCNGG 5 cut(s) 15, 147, 148, 318, 491
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 2 cut(s) 318, 491
MvnI CGCG 2 cut(s) 237, 432
MwoI GCNNNNNNNGC 7 cut(s) 228, 231, 392, 528, 704, 710, 824
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 3 cut(s) 15, 147, 148
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 898
NdeII GATC 5 cut(s) 207, 289, 502, 665, 938
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 4 cut(s) 127, 142, 1002, 1012
NlaIV GGNNCC 1 cut(s) 1064
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 589
NspI RCATGY 1 cut(s) 1002
PaeR7I CTCGAG 2 cut(s) 293, 822
PciI ACATGT 1 cut(s) 998
PctI GAATGC 1 cut(s) 483
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 37, 757, 851
PinAI ACCGGT 1 cut(s) 368
PkrI GCNGC 9 cut(s) 183, 224, 233, 236, 355, 524, 706, 820, 1099
PleI GAGTC 2 cut(s) 44, 367
PpsI GAGTC 2 cut(s) 44, 367
PscI ACATGT 1 cut(s) 998
PsiI TTATAA 1 cut(s) 915
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 316, 489
PspGI CCWGG 2 cut(s) 316, 489
PspN4I GGNNCC 1 cut(s) 1064
PspPI GGNCC 2 cut(s) 127, 340
PspXI VCTCGAGB 1 cut(s) 822
PstNI CAGNNNCTG 2 cut(s) 638, 704
PsuI RGATCY 3 cut(s) 289, 502, 665
RsaI GTAC 2 cut(s) 47, 71
RsaNI GTAC 2 cut(s) 46, 70
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 3 cut(s) 762, 836, 1120
SatI GCNGC 9 cut(s) 182, 223, 232, 235, 354, 523, 705, 819, 1098
Sau3AI GATC 5 cut(s) 207, 289, 502, 665, 938
Sau96I GGNCC 2 cut(s) 127, 340
SchI GAGTC 2 cut(s) 44, 368
ScrFI CCNGG 5 cut(s) 15, 147, 148, 318, 491
SduI GDGCHC 3 cut(s) 300, 979, 1019
SfaNI GCATC 5 cut(s) 460, 532, 691, 876, 929
SfcI CTRYAG 1 cut(s) 639
Sfr274I CTCGAG 2 cut(s) 293, 822
SinI GGWCC 2 cut(s) 127, 340
SlaI CTCGAG 2 cut(s) 293, 822
SmaI CCCGGG 1 cut(s) 148
SmlI CTYRAG 5 cut(s) 5, 293, 567, 761, 822
SmoI CTYRAG 5 cut(s) 5, 293, 567, 761, 822
Sse9I AATT 4 cut(s) 24, 102, 154, 613
SsiI CCGC 4 cut(s) 235, 430, 481, 535
SspI AATATT 1 cut(s) 629
SspMI CTAG 2 cut(s) 608, 815
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 5 cut(s) 13, 145, 146, 316, 489
StyI CCWWGG 4 cut(s) 123, 336, 402, 655
TaaI ACNGT 3 cut(s) 553, 640, 887
TaiI ACGT 3 cut(s) 138, 246, 415
TaqI TCGA 5 cut(s) 213, 255, 294, 378, 823
TasI AATT 4 cut(s) 24, 102, 154, 613
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 37, 757, 851
Tru1I TTAA 3 cut(s) 762, 836, 1120
Tru9I TTAA 3 cut(s) 762, 836, 1120
TscAI CASTG 1 cut(s) 590
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 8 cut(s) 181, 222, 231, 353, 522, 704, 818, 1097
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 3 cut(s) 770, 1025, 1068
TspMI CCCGGG 1 cut(s) 146
TspRI CASTG 1 cut(s) 590
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 1015
VpaK11BI GGWCC 2 cut(s) 127, 340
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 1002
XhoI CTCGAG 2 cut(s) 293, 822
XmaI CCCGGG 1 cut(s) 146
XspI CTAG 2 cut(s) 608, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.