MD14G1000200.v1.1

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001988
Physical Location & Seq
Reverse (-)
44459 .. 47893
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1000200.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGCTCAAGCGTCCCGGCAGAGGAATCGGAATGGGGAGTCCCCAGTACGACTCCCTCTCCGACAAAATGTACAGATACAGGAGCGTCATCCTCGTCATCTCAATCCCTCTCCTCCTCCTTACCATGGTCCTTTACGTCATGCCCCCCGGCGGTAATTTGCGCAACCAGCTCCCCCGCAAGCTGCCTTCCAAGACATCCTACGCTGTGATCTTCGACGCCGGCAGCTCCGGCAGCCGCATCCACGTCTTCTGCTTCGACCACAACCTCCAGCTCCTTCCCATTGGCAAAGATCTCGAGCTCTTTCTTCAGGTCAAGCCTGGTCTGAGCGCTTATGCCAAGGACCCTCGCTCTGCTGCCGAGTCCCTCACCGGTCTTCTCGATAAGGCTGAGAGCGCAGTTCCGAAGGAGCTTCGTTCCTTCACTCCCGTCCGCGTTGGGGCTACGGCTGGCCTTAGGGCTTTGGAGGGCGATGCTTCTGACCGCATTCTCCAGGCCGTCAGGGATCTGCTCAAACAAAGAAGCAGCCTCAGCTCCCGCCCTGATGCCGTTACTGTAATTGATGGAACTCAAGAAGGTTCCTTCCAGTGGGTGACAATAAACTATCTACTAGGAAACTTGGGGAAGAAATATTCAGATACTGTAGGGGTGGTTGACCTTGGAGGTGGATCTGTTCAGATGGCTTATGCTATCTCAGAGGCAGATGCTGCAAAAGCACCCAAGTTATCAGATGGAGAGGATGTATATGTAAGGCAAATGAATCTTAAAGGCGTTAAATATAACCTTTATGTTCACAGTTACTTGCATTACGGGTTACTAGCAGCTCGAGCAGAGATTTTAAAGGTTTCTGAAGATTCTGGCAACCCCTGCATCTTAGCCGGATATTATGGTTCTTACGCATATGGAGGACAATCTTATAAAGCATCAGCTTCCTCATCTGGATCAAGTGTTGAGGAGTGCAGGAGGGTAGTTACGAAGGCACTCAAAGTAAATGAAGCAACATGTTCACACATGAAGTGCACATTTGGCGGGGTATGGAACGGTGGAGGAGGGGATGGACAAAGGAACCTATTTGTGGCTTCATTTTTCTTTGACAGAGCTGCTGAGGCTGGTTTTGTTAACCCAAACGAACCTATTGCAAAAGTTCACCCTGGTAACTTTGAGGAGGCAGCTAAGCGTGCTTGTGAAACTAAACTTGAGGATGCTAAATCAACGTATCCAAGAGTTGAGGAGGGTAACCTGCCGTTCTTGTGCATGGATCTTGTTTACCAATCTTCATTGCTTGTGGATGGATTTGGTCTTGATCCCTGGCAAGATATTACATTGGTGAAGAAAGTTAAATATCAAAATTCCTTGGTTGAAGCAGCATGGCCACTAGGGAGTGCCATAGAGGCCGTGTCCTCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 map00230 map00240
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

469

Amino Acids

50.7

Weight (kDa)

8.29

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDA1_CD39 PF01150 63 - 466 1.6e-106 GDA1/CD39 (nucleoside phosphatase) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 915, 1405
Acc16I TGCGCA 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 1245
AccII CGCG 1 cut(s) 432
AciI CCGC 7 cut(s) 150, 175, 235, 430, 481, 535, 1026
AclWI GGATC 5 cut(s) 510, 673, 946, 1263, 1295
AcoI YGGCCR 1 cut(s) 1367
AcsI RAATTY 1 cut(s) 1345
AcuI CTGAAG 2 cut(s) 290, 867
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 47, 71
AfeI AGCGCT 1 cut(s) 328
AfiI CCNNNNNNNGG 6 cut(s) 20, 124, 149, 436, 585, 1072
AflIII ACRYGT 1 cut(s) 998
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 1 cut(s) 1358
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 4 cut(s) 316, 489, 1147, 1304
Alw21I GWGCWC 2 cut(s) 300, 1019
Alw44I GTGCAC 1 cut(s) 1015
AlwI GGATC 5 cut(s) 510, 673, 946, 1263, 1295
AlwNI CAGNNNCTG 2 cut(s) 638, 704
Ama87I CYCGRG 2 cut(s) 293, 822
Aor51HI AGCGCT 1 cut(s) 328
AoxI GGCC 4 cut(s) 448, 492, 1367, 1389
ApaLI GTGCAC 1 cut(s) 1015
ApoI RAATTY 1 cut(s) 1345
AsiGI ACCGGT 1 cut(s) 368
AspLEI GCGC 3 cut(s) 162, 329, 395
AspS9I GGNCC 2 cut(s) 127, 340
AsuC2I CCSGG 2 cut(s) 15, 147
AsuHPI GGTGA 4 cut(s) 358, 601, 1136, 1336
AvaI CYCGRG 2 cut(s) 293, 822
AvaII GGWCC 2 cut(s) 127, 340
AxyI CCTNAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 1019
BalI TGGCCA 1 cut(s) 1369
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 238, 365
Bbv12I GWGCWC 2 cut(s) 300, 1019
BbvCI CCTCAGC 2 cut(s) 527, 1101
BccI CCATC 5 cut(s) 554, 670, 722, 1046, 1280
BceAI ACGGC 5 cut(s) 459, 479, 530, 1225, 1376
BcgI CGANNNNNNTGC 6 cut(s) 7, 41, 274, 308, 1115, 1149
BciT130I CCWGG 4 cut(s) 318, 491, 1149, 1306
BciVI GTATCC 1 cut(s) 1224
BcnI CCSGG 2 cut(s) 15, 147
BfaI CTAG 3 cut(s) 608, 815, 1373
BfmI CTRYAG 1 cut(s) 639
BfoI RGCGCY 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 1245
BfuI GTATCC 1 cut(s) 1224
BglI GCCNNNNNGGC 1 cut(s) 1388
BglII AGATCT 1 cut(s) 289
BlpI GCTNAGC 1 cut(s) 1170
Bme1390I CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
Bme18I GGWCC 2 cut(s) 127, 340
BmeT110I CYCGRG 2 cut(s) 293, 822
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 127, 340
BmiI GGNNCC 3 cut(s) 342, 577, 1064
BmrFI CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 7 cut(s) 246, 460, 532, 691, 876, 929, 1189
BmuI ACTGGG 1 cut(s) 37
BpiI GAAGAC 2 cut(s) 238, 365
BpmI CTGGAG 2 cut(s) 251, 473
Bpu10I CCTNAGC 2 cut(s) 527, 1101
Bpu1102I GCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 2 cut(s) 552, 1214
BpuMI CCSGG 2 cut(s) 15, 147
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 7 cut(s) 123, 145, 336, 655, 1147, 1304, 1350
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 723, 753
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 124, 149, 436, 585, 1072
Bse118I RCCGGY 2 cut(s) 218, 368
Bse1I ACTGG 2 cut(s) 43, 583
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 1274
BseBI CCWGG 4 cut(s) 318, 491, 1149, 1306
BseDI CCNNGG 7 cut(s) 123, 145, 336, 655, 1147, 1304, 1350
BseGI GGATG 7 cut(s) 87, 194, 237, 742, 1057, 1204, 1291
BseLI CCNNNNNNNGG 6 cut(s) 20, 124, 149, 436, 585, 1072
BseMI GCAATG 1 cut(s) 1274
BseMII CTCAG 5 cut(s) 314, 378, 541, 705, 1092
BseNI ACTGG 2 cut(s) 43, 583
BseRI GAGGAG 6 cut(s) 101, 104, 965, 1059, 1175, 1241
BseSI GKGCMC 1 cut(s) 1019
BsgI GTGCAG 1 cut(s) 976
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 450, 494, 1369, 1391
BshTI ACCGGT 1 cut(s) 368
BsiHKAI GWGCWC 2 cut(s) 300, 1019
BsiHKCI CYCGRG 2 cut(s) 293, 822
BsiSI CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
BslFI GGGAC 2 cut(s) 24, 346
BslI CCNNNNNNNGG 6 cut(s) 20, 124, 149, 436, 585, 1072
BsmFI GGGAC 2 cut(s) 24, 346
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 4 cut(s) 450, 494, 1369, 1391
BsoBI CYCGRG 2 cut(s) 293, 822
Bsp1286I GDGCHC 2 cut(s) 300, 1019
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
Bsp1720I GCTNAGC 1 cut(s) 1170
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 7 cut(s) 150, 175, 235, 430, 481, 535, 1026
BspANI GGCC 4 cut(s) 450, 494, 1369, 1391
BspCNI CTCAG 5 cut(s) 315, 379, 540, 704, 1093
BspFNI CGCG 1 cut(s) 432
BspLI GGNNCC 3 cut(s) 342, 577, 1064
BspMI ACCTGC 1 cut(s) 1245
BspPI GGATC 5 cut(s) 510, 673, 946, 1263, 1295
BsrDI GCAATG 1 cut(s) 1274
BsrFI RCCGGY 2 cut(s) 218, 368
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 2 cut(s) 43, 583
BssAI RCCGGY 2 cut(s) 218, 368
BssECI CCNNGG 7 cut(s) 123, 145, 336, 655, 1147, 1304, 1350
BssMI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 4 cut(s) 123, 336, 655, 1350
Bst2UI CCWGG 4 cut(s) 318, 491, 1149, 1306
Bst4CI ACNGT 4 cut(s) 553, 640, 794, 1040
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 2 cut(s) 704, 864
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 4 cut(s) 179, 220, 448, 1176
BstDEI CTNAG 8 cut(s) 323, 387, 452, 527, 691, 871, 1101, 1170
BstDSI CCRYGG 1 cut(s) 123
BstEII GGTNACC 1 cut(s) 1232
BstF5I GGATG 7 cut(s) 87, 194, 237, 742, 1057, 1204, 1291
BstFNI CGCG 1 cut(s) 432
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 162, 329, 395
BstKTI GATC 7 cut(s) 210, 292, 505, 668, 941, 1258, 1303
BstMBI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
BstNI CCWGG 4 cut(s) 318, 491, 1149, 1306
BstNSI RCATGY 1 cut(s) 1002
BstPI GGTNACC 1 cut(s) 1232
BstSCI CCNGG 6 cut(s) 13, 145, 316, 489, 1147, 1304
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 1019
BstUI CGCG 1 cut(s) 432
BstV2I GAAGAC 2 cut(s) 238, 365
BstX2I RGATCY 4 cut(s) 289, 502, 665, 1255
BstYI RGATCY 4 cut(s) 289, 502, 665, 1255
Bsu36I CCTNAGG 1 cut(s) 452
BsuI GTATCC 1 cut(s) 1224
BsuRI GGCC 4 cut(s) 450, 494, 1369, 1391
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 7 cut(s) 87, 194, 237, 742, 1057, 1204, 1291
BtsIMutI CAGTG 1 cut(s) 590
BveI ACCTGC 1 cut(s) 1245
Cac8I GCNNGC 4 cut(s) 179, 220, 448, 1176
CaiI CAGNNNCTG 2 cut(s) 638, 704
CfoI GCGC 3 cut(s) 162, 329, 395
Cfr10I RCCGGY 2 cut(s) 218, 368
Cfr13I GGNCC 2 cut(s) 127, 340
CseI GACGC 2 cut(s) 73, 224
Csp6I GTAC 2 cut(s) 46, 70
CspAI ACCGGT 1 cut(s) 368
CviAII CATG 6 cut(s) 124, 139, 999, 1009, 1252, 1365
CviQI GTAC 2 cut(s) 46, 70
DdeI CTNAG 8 cut(s) 323, 387, 452, 527, 691, 871, 1101, 1170
DpnI GATC 7 cut(s) 209, 291, 504, 667, 940, 1257, 1302
DpnII GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
DraI TTTAAA 1 cut(s) 837
EaeI YGGCCR 1 cut(s) 1367
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 4 cut(s) 123, 336, 655, 1350
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 127, 340
Eco47III AGCGCT 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 290, 867
Eco81I CCTNAGG 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 293, 822
Eco91I GGTNACC 1 cut(s) 1232
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 340
EcoO65I GGTNACC 1 cut(s) 1232
EcoRII CCWGG 4 cut(s) 316, 489, 1147, 1304
EcoT14I CCWWGG 4 cut(s) 123, 336, 655, 1350
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 4 cut(s) 123, 336, 655, 1350
FaeI CATG 6 cut(s) 127, 142, 1002, 1012, 1255, 1368
FaqI GGGAC 2 cut(s) 24, 346
FatI CATG 6 cut(s) 123, 138, 998, 1008, 1251, 1364
FauI CCCGC 3 cut(s) 182, 542, 1019
FauNDI CATATG 1 cut(s) 898
FokI GGATG 7 cut(s) 74, 181, 224, 749, 1064, 1211, 1298
FriOI GRGCYC 1 cut(s) 300
FspBI CTAG 3 cut(s) 608, 815, 1373
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 3 cut(s) 161, 328, 394
GsuI CTGGAG 2 cut(s) 251, 473
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 4 cut(s) 450, 494, 1369, 1391
HapII CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
HgaI GACGC 2 cut(s) 73, 224
HhaI GCGC 3 cut(s) 162, 329, 395
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 6 cut(s) 127, 142, 1002, 1012, 1255, 1368
Hin6I GCGC 3 cut(s) 160, 327, 393
HinP1I GCGC 3 cut(s) 160, 327, 393
HincII GTYRAC 2 cut(s) 652, 1117
HindII GTYRAC 2 cut(s) 652, 1117
HinfI GANTC 6 cut(s) 24, 37, 50, 359, 757, 851
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
HphI GGTGA 4 cut(s) 358, 601, 1136, 1336
Hpy166II GTNNAC 7 cut(s) 652, 790, 1004, 1017, 1117, 1144, 1264
Hpy188III TCNNGA 5 cut(s) 293, 377, 569, 936, 1298
Hpy8I GTNNAC 7 cut(s) 652, 790, 1004, 1017, 1117, 1144, 1264
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 7 cut(s) 195, 284, 397, 427, 566, 589, 967
HpyCH4III ACNGT 4 cut(s) 553, 640, 794, 1040
HpyCH4IV ACGT 3 cut(s) 135, 243, 1211
HpyCH4V TGCA 7 cut(s) 707, 802, 867, 957, 1017, 1136, 1251
HpyF3I CTNAG 8 cut(s) 323, 387, 452, 527, 691, 871, 1101, 1170
HpySE526I ACGT 3 cut(s) 135, 243, 1211
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 6 cut(s) 127, 142, 1002, 1012, 1255, 1368
HspAI GCGC 3 cut(s) 160, 327, 393
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 1117
Kzo9I GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
LmnI GCTCC 6 cut(s) 81, 174, 230, 276, 406, 536
LweI GCATC 7 cut(s) 246, 460, 532, 691, 876, 929, 1189
MaeI CTAG 3 cut(s) 608, 815, 1373
MaeII ACGT 3 cut(s) 135, 243, 1211
MaeIII GTNAC 7 cut(s) 547, 589, 794, 810, 967, 1151, 1232
MalI GATC 7 cut(s) 209, 291, 504, 667, 940, 1257, 1302
MboI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
MboII GAAGA 8 cut(s) 202, 238, 296, 365, 634, 860, 1263, 1339
MflI RGATCY 4 cut(s) 289, 502, 665, 1255
MhlI GDGCHC 2 cut(s) 300, 1019
MlsI TGGCCA 1 cut(s) 1369
MluCI AATT 3 cut(s) 154, 555, 1345
MluNI TGGCCA 1 cut(s) 1369
MlyI GAGTC 3 cut(s) 44, 46, 368
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 1369
MroNI GCCGGC 1 cut(s) 218
MscI TGGCCA 1 cut(s) 1369
MseI TTAA 5 cut(s) 762, 771, 836, 1116, 1335
Msp20I TGGCCA 1 cut(s) 1369
MspI CCGG 6 cut(s) 15, 147, 219, 228, 369, 876
MspR9I CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 4 cut(s) 318, 491, 1149, 1306
MvnI CGCG 1 cut(s) 432
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 2 cut(s) 15, 147
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 898
NdeII GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 6 cut(s) 127, 142, 1002, 1012, 1255, 1368
NlaIV GGNNCC 3 cut(s) 342, 577, 1064
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 589
NsbI TGCGCA 1 cut(s) 161
NspI RCATGY 1 cut(s) 1002
PaeR7I CTCGAG 2 cut(s) 293, 822
PciI ACATGT 1 cut(s) 998
PctI GAATGC 1 cut(s) 483
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 24, 757, 851
PinAI ACCGGT 1 cut(s) 368
PleI GAGTC 3 cut(s) 44, 45, 367
PpsI GAGTC 3 cut(s) 44, 45, 367
PpuMI RGGWCCY 1 cut(s) 340
PscI ACATGT 1 cut(s) 998
PsiI TTATAA 2 cut(s) 915, 1405
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 4 cut(s) 316, 489, 1147, 1304
PspEI GGTNACC 1 cut(s) 1232
PspGI CCWGG 4 cut(s) 316, 489, 1147, 1304
PspN4I GGNNCC 3 cut(s) 342, 577, 1064
PspPI GGNCC 2 cut(s) 127, 340
PspPPI RGGWCCY 1 cut(s) 340
PspXI VCTCGAGB 1 cut(s) 822
PstNI CAGNNNCTG 2 cut(s) 638, 704
PsuI RGATCY 4 cut(s) 289, 502, 665, 1255
RsaI GTAC 2 cut(s) 47, 71
RsaNI GTAC 2 cut(s) 46, 70
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 5 cut(s) 762, 771, 836, 1116, 1335
Sau3AI GATC 7 cut(s) 207, 289, 502, 665, 938, 1255, 1300
Sau96I GGNCC 2 cut(s) 127, 340
SchI GAGTC 3 cut(s) 44, 46, 368
ScrFI CCNGG 6 cut(s) 15, 147, 318, 491, 1149, 1306
SduI GDGCHC 2 cut(s) 300, 1019
SfaNI GCATC 7 cut(s) 246, 460, 532, 691, 876, 929, 1189
SfcI CTRYAG 1 cut(s) 639
Sfr274I CTCGAG 2 cut(s) 293, 822
SinI GGWCC 2 cut(s) 127, 340
SlaI CTCGAG 2 cut(s) 293, 822
SmlI CTYRAG 5 cut(s) 5, 293, 567, 822, 1193
SmoI CTYRAG 5 cut(s) 5, 293, 567, 822, 1193
Sse9I AATT 3 cut(s) 154, 555, 1345
SsiI CCGC 7 cut(s) 150, 175, 235, 430, 481, 535, 1026
SspI AATATT 1 cut(s) 629
SspMI CTAG 3 cut(s) 608, 815, 1373
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 6 cut(s) 13, 145, 316, 489, 1147, 1304
StyI CCWWGG 4 cut(s) 123, 336, 655, 1350
TaaI ACNGT 4 cut(s) 553, 640, 794, 1040
TaiI ACGT 3 cut(s) 138, 246, 1214
TaqI TCGA 5 cut(s) 213, 255, 294, 378, 823
TasI AATT 3 cut(s) 154, 555, 1345
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 24, 757, 851
Tru1I TTAA 5 cut(s) 762, 771, 836, 1116, 1335
Tru9I TTAA 5 cut(s) 762, 771, 836, 1116, 1335
TscAI CASTG 1 cut(s) 590
TseFI GTSAC 1 cut(s) 589
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 5 cut(s) 770, 1005, 1025, 1068, 1263
TspRI CASTG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 1015
VpaK11BI GGWCC 2 cut(s) 127, 340
XapI RAATTY 1 cut(s) 1345
XceI RCATGY 1 cut(s) 1002
XhoI CTCGAG 2 cut(s) 293, 822
XspI CTAG 3 cut(s) 608, 815, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.