FvH4_4g17940

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002436
Physical Location & Seq
Forward (+)
21921972 .. 21923706
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17940.t2

Sequence Viewer

Length: 879 bp
ATGTCTGCTCAAGAAGCCTCAGAGCTACTCAAATGTCAGACATGGGTCTTGAAAGTTTTTATCCACTGTGAAGGTTGCCAGAGGCGAGTCAAGAAAGTTCTACAGAGAATTGATGGTGTTTACACCACATCTATTGAGGCAGAGCAAAGCAGAGTCACAGTCACTGGAAACGTCGACGTTCAGACCCTCATCAAGAAGCTGATTGTAAGAACTGGTAAGCATGCCGAACCATGGCCAGAAAATCTGACCACGAAAGAAAAGACCAAGCCTGTGAAAGTCAAGAGCAATGGTAAACAGAAATCCTCAGATATTTGCCAAAGTCAGCGGAGTACTACATGTGACAAAGTTGGAGCGCTCGGGCTAAACAGAACAAGTGAGGGTGACACAACTAGGTTTTACAGAGAGGAACAATGCCAAGTGTCAAAGAAGGGTCACATTCTGCCGAAGAAATCTCTGAATGTTCATGATTCTCAGGCCATGGATCTTCAGCTCAGTGAAAGTGAAAAAGGGAGTGTTAGTGGCAAAAAGACCAGGAATGGCCAAAGATGTTATTTGGGTTCAACAAGCTGTGGTGCACCTGCAAGCATAGTTTCTCAACATTCTAATGATCACAATGTTGGTCCGGTTGATCTCAGCCATATCATCGGAAACGTGTATCCGATGGACTACCACTCTCATTTGGTGTTCAATGGGAGTGACATTACAGCAGCTGCTACATCACTGCGTAGCATGAGCTTGGCTCCTTACTATTGTTATGGTCCATCGTCACAGTACAAGGGTGCAGCTAATGGTGAACAGATTGATCAAGTCAATGCAACATATCTTAACAGCCTGCAGATTTTCAGTGATGACGATGCTAATGGCTGTTTTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

293

Amino Acids

32.11

Weight (kDa)

8.71

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 83 2e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 586
Acc36I ACCTGC 1 cut(s) 586
AccI GTMKAC 1 cut(s) 174
AciI CCGC 1 cut(s) 325
AclWI GGATC 1 cut(s) 489
AcoI YGGCCR 2 cut(s) 233, 538
AcuI CTGAAG 1 cut(s) 470
AfaI GTAC 2 cut(s) 331, 773
AfeI AGCGCT 1 cut(s) 354
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 2 cut(s) 335, 651
AgsI TTSAA 3 cut(s) 52, 561, 688
AjnI CCWGG 1 cut(s) 530
AluBI AGCT 7 cut(s) 25, 199, 490, 567, 710, 735, 785
AluI AGCT 7 cut(s) 25, 199, 490, 567, 710, 735, 785
Alw21I GWGCWC 1 cut(s) 577
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 1 cut(s) 489
AlwNI CAGNNNCTG 2 cut(s) 164, 710
Ama87I CYCGRG 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 354
AoxI GGCC 3 cut(s) 233, 474, 538
ApaLI GTGCAC 1 cut(s) 573
ApeKI GCWGC 3 cut(s) 707, 710, 782
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 2 cut(s) 620, 758
AsuHPI GGTGA 2 cut(s) 392, 803
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 2 cut(s) 620, 758
BaeGI GKGCMC 1 cut(s) 577
BalI TGGCCA 2 cut(s) 235, 540
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 697, 719, 794
BccI CCATC 3 cut(s) 107, 655, 769
BciT130I CCWGG 1 cut(s) 532
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 2 cut(s) 607, 802
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 2 cut(s) 101, 833
BfoI RGCGCY 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 586
BfuI GTATCC 1 cut(s) 666
BisI GCNGC 3 cut(s) 708, 711, 783
BlsI GCNGC 3 cut(s) 709, 712, 784
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 1 cut(s) 532
Bme18I GGWCC 2 cut(s) 620, 758
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 2 cut(s) 620, 758
BmiI GGNNCC 1 cut(s) 741
BmrFI CCNGG 1 cut(s) 532
BmsI GCATC 1 cut(s) 844
BoxI GACNNNNGTC 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 724, 756
BsaJI CCNNGG 2 cut(s) 230, 477
BsaWI WCCGGW 1 cut(s) 622
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 169, 217
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 1 cut(s) 532
BseDI CCNNGG 2 cut(s) 230, 477
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 5 cut(s) 33, 318, 485, 505, 646
BseNI ACTGG 2 cut(s) 169, 217
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 3 cut(s) 697, 719, 794
BsgI GTGCAG 1 cut(s) 801
BshFI GGCC 3 cut(s) 235, 476, 540
BsiHKAI GWGCWC 1 cut(s) 577
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 623
BslI CCNNNNNNNGG 1 cut(s) 231
BsnI GGCC 3 cut(s) 235, 476, 540
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 4 cut(s) 481, 607, 628, 802
Bsp19I CCATGG 2 cut(s) 230, 477
BspACI CCGC 1 cut(s) 325
BspANI GGCC 3 cut(s) 235, 476, 540
BspCNI CTCAG 5 cut(s) 32, 317, 484, 504, 645
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 741
BspMAI CTGCAG 1 cut(s) 837
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 1 cut(s) 489
BsrDI GCAATG 1 cut(s) 292
BsrI ACTGG 2 cut(s) 169, 217
BssECI CCNNGG 2 cut(s) 230, 477
BssMI GATC 4 cut(s) 481, 607, 628, 802
BssT1I CCWWGG 2 cut(s) 230, 477
Bst2UI CCWGG 1 cut(s) 532
Bst4CI ACNGT 3 cut(s) 68, 160, 771
BstC8I GCNNGC 3 cut(s) 222, 583, 833
BstDEI CTNAG 5 cut(s) 19, 304, 471, 491, 632
BstDSI CCRYGG 2 cut(s) 230, 477
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 4 cut(s) 484, 610, 631, 805
BstMBI GATC 4 cut(s) 481, 607, 628, 802
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 1 cut(s) 532
BstNSI RCATGY 2 cut(s) 224, 339
BstPAI GACNNNNGTC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 530
BstSFI CTRYAG 2 cut(s) 101, 833
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 3 cut(s) 697, 719, 794
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 235, 476, 540
BtgI CCRYGG 2 cut(s) 230, 477
BtsI GCAGTG 1 cut(s) 719
BtsIMutI CAGTG 5 cut(s) 64, 162, 499, 719, 850
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 3 cut(s) 222, 583, 833
CaiI CAGNNNCTG 2 cut(s) 164, 710
CciI TCATGA 1 cut(s) 463
CfoI GCGC 1 cut(s) 355
Cfr13I GGNCC 2 cut(s) 620, 758
Csp6I GTAC 2 cut(s) 330, 772
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 8 cut(s) 42, 221, 231, 336, 464, 478, 730, 874
CviQI GTAC 2 cut(s) 330, 772
DdeI CTNAG 5 cut(s) 19, 304, 471, 491, 632
DpnI GATC 4 cut(s) 483, 609, 630, 804
DpnII GATC 4 cut(s) 481, 607, 628, 802
EaeI YGGCCR 2 cut(s) 233, 538
Eco130I CCWWGG 2 cut(s) 230, 477
Eco47I GGWCC 2 cut(s) 620, 758
Eco47III AGCGCT 1 cut(s) 354
Eco57I CTGAAG 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 530
EcoT14I CCWWGG 2 cut(s) 230, 477
ErhI CCWWGG 2 cut(s) 230, 477
FaeI CATG 8 cut(s) 45, 224, 234, 339, 467, 481, 733, 877
FatI CATG 8 cut(s) 41, 220, 230, 335, 463, 477, 729, 873
FbaI TGATCA 2 cut(s) 607, 802
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 708, 711, 783
Fsp4HI GCNGC 3 cut(s) 708, 711, 783
FspBI CTAG 1 cut(s) 390
GlaI GCGC 1 cut(s) 354
GluI GCNGC 3 cut(s) 708, 711, 783
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 3 cut(s) 235, 476, 540
HapII CCGG 1 cut(s) 623
HhaI GCGC 1 cut(s) 355
Hin1II CATG 8 cut(s) 45, 224, 234, 339, 467, 481, 733, 877
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 3 cut(s) 87, 153, 467
HpaII CCGG 1 cut(s) 623
HphI GGTGA 2 cut(s) 392, 803
Hpy166II GTNNAC 5 cut(s) 121, 175, 293, 575, 794
Hpy188I TCNGA 8 cut(s) 22, 39, 183, 246, 307, 456, 647, 660
Hpy188III TCNNGA 6 cut(s) 11, 49, 91, 193, 280, 464
Hpy8I GTNNAC 5 cut(s) 121, 175, 293, 575, 794
Hpy99I CGWCG 2 cut(s) 176, 179
HpyAV CCTTC 2 cut(s) 65, 421
HpyCH4III ACNGT 3 cut(s) 68, 160, 771
HpyCH4IV ACGT 3 cut(s) 171, 177, 651
HpyCH4V TGCA 5 cut(s) 575, 581, 782, 815, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 5 cut(s) 19, 304, 471, 491, 632
HpySE526I ACGT 3 cut(s) 171, 177, 651
Hsp92II CATG 8 cut(s) 45, 224, 234, 339, 467, 481, 733, 877
HspAI GCGC 1 cut(s) 353
Ksp22I TGATCA 2 cut(s) 607, 802
Kzo9I GATC 4 cut(s) 481, 607, 628, 802
LmnI GCTCC 2 cut(s) 350, 745
Lsp1109I GCAGC 3 cut(s) 697, 719, 794
LweI GCATC 1 cut(s) 844
MaeI CTAG 1 cut(s) 390
MaeII ACGT 3 cut(s) 171, 177, 651
MaeIII GTNAC 7 cut(s) 154, 160, 338, 380, 431, 695, 765
MalI GATC 4 cut(s) 483, 609, 630, 804
MboI GATC 4 cut(s) 481, 607, 628, 802
MboII GAAGA 2 cut(s) 457, 476
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 1 cut(s) 577
MlsI TGGCCA 2 cut(s) 235, 540
MluCI AATT 1 cut(s) 108
MluNI TGGCCA 2 cut(s) 235, 540
MlyI GAGTC 2 cut(s) 96, 162
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 7 cut(s) 28, 75, 130, 197, 313, 370, 397
Mox20I TGGCCA 2 cut(s) 235, 540
MscI TGGCCA 2 cut(s) 235, 540
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 603
Msp20I TGGCCA 2 cut(s) 235, 540
MspA1I CMGCKG 2 cut(s) 325, 710
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 1 cut(s) 532
MvaI CCWGG 1 cut(s) 532
MwoI GCNNNNNNNGC 1 cut(s) 14
NcoI CCATGG 2 cut(s) 230, 477
NdeII GATC 4 cut(s) 481, 607, 628, 802
NlaIII CATG 8 cut(s) 45, 224, 234, 339, 467, 481, 733, 877
NlaIV GGNNCC 1 cut(s) 741
NmuCI GTSAC 7 cut(s) 154, 160, 338, 380, 431, 695, 765
NspI RCATGY 2 cut(s) 224, 339
PaeI GCATGC 1 cut(s) 224
PagI TCATGA 1 cut(s) 463
PaqCI CACCTGC 1 cut(s) 586
PciI ACATGT 1 cut(s) 335
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 3 cut(s) 709, 712, 784
PleI GAGTC 2 cut(s) 95, 161
PpsI GAGTC 2 cut(s) 95, 161
PscI ACATGT 1 cut(s) 335
PshAI GACNNNNGTC 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 530
PspGI CCWGG 1 cut(s) 530
PspN4I GGNNCC 1 cut(s) 741
PspPI GGNCC 2 cut(s) 620, 758
PstI CTGCAG 1 cut(s) 837
PstNI CAGNNNCTG 2 cut(s) 164, 710
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 2 cut(s) 331, 773
RsaNI GTAC 2 cut(s) 330, 772
RseI CAYNNNNRTG 1 cut(s) 603
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 3 cut(s) 708, 711, 783
Sau3AI GATC 4 cut(s) 481, 607, 628, 802
Sau96I GGNCC 2 cut(s) 620, 758
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 2 cut(s) 96, 162
ScrFI CCNGG 1 cut(s) 532
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 2 cut(s) 101, 833
SgrDI CGTCGACG 1 cut(s) 173
SinI GGWCC 2 cut(s) 620, 758
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
SphI GCATGC 1 cut(s) 224
Sse9I AATT 1 cut(s) 108
SsiI CCGC 1 cut(s) 325
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 1 cut(s) 530
StyI CCWWGG 2 cut(s) 230, 477
TaaI ACNGT 3 cut(s) 68, 160, 771
TaiI ACGT 3 cut(s) 174, 180, 654
TaqI TCGA 1 cut(s) 174
TasI AATT 1 cut(s) 108
TatI WGTACW 2 cut(s) 329, 771
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 5 cut(s) 71, 169, 499, 726, 850
TseFI GTSAC 7 cut(s) 154, 160, 338, 380, 431, 695, 765
TseI GCWGC 3 cut(s) 707, 710, 782
Tsp45I GTSAC 7 cut(s) 154, 160, 338, 380, 431, 695, 765
TspDTI ATGAA 1 cut(s) 452
TspRI CASTG 5 cut(s) 71, 169, 499, 726, 850
VneI GTGCAC 1 cut(s) 573
VpaK11BI GGWCC 2 cut(s) 620, 758
XceI RCATGY 2 cut(s) 224, 339
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 390
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.