MD16G1170500.v1.1

CAP-Gly domain-containing linker protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002436
Physical Location & Seq
Reverse (-)
14200367 .. 14202507
2141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1170500.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCCGCGGCCAACTCTTCTGATCCCTCGATTCCCTCCACTCCTCCTCTCCCCGCCCCTGCTCCTCCACTCTCCTCCAGCAAGGAGAATGTGACCCCGATTGGCTCCAAGCTTGCGGAATTGAATGAATCGAGGTCTGAGCTACTTGGTAGAATTCAAGGGTTGAAGCAGGATTTGCAAAGTTGGAGGTCAAAGTTAGACACTCAAGTGAAAGTCTATCGCGACGAGCTTTCAGAACTCAAGAAATCCCTCAACACCGAAGTGGATCAACTTCGATCTGAATTTCAAGAACTGAGGACCACTCTTCAGCAGCAGCAAGAGGATGTTACAACTAGTCTTAGAAACTTGGGGCTGCAGGATGTCTCAGGAGAGAAAAAAGAAGCCCAAGATACCGAAGAACCAAATGCTTCAACCAAGGTGGATGAAGCCAAAGAAGCCGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.3

Weight (kDa)

4.69

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG16 PF08614 17 - 116 9e-06 Autophagy protein 16 (ATG16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 8, 222
AciI CCGC 4 cut(s) 6, 8, 54, 116
AclWI GGATC 2 cut(s) 17, 273
AcoI YGGCCR 2 cut(s) 3, 9
AcsI RAATTY 2 cut(s) 153, 281
AcuI CTGAAG 1 cut(s) 290
AgsI TTSAA 5 cut(s) 124, 158, 166, 287, 411
AhlI ACTAGT 1 cut(s) 332
AleI CACNNNNGTG 2 cut(s) 206, 260
AluBI AGCT 4 cut(s) 112, 142, 229, 444
AluI AGCT 4 cut(s) 112, 142, 229, 444
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 2 cut(s) 17, 273
AoxI GGCC 2 cut(s) 3, 9
ApeKI GCWGC 3 cut(s) 310, 313, 352
ApoI RAATTY 2 cut(s) 153, 281
AspS9I GGNCC 1 cut(s) 297
AvaII GGWCC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 322, 325, 339
BcoDI GTCTC 1 cut(s) 367
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 2 cut(s) 333, 445
BfmI CTRYAG 1 cut(s) 353
BisI GCNGC 5 cut(s) 6, 9, 311, 314, 353
BlsI GCNGC 5 cut(s) 7, 10, 312, 315, 354
Bme18I GGWCC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 286, 318
BpmI CTGGAG 1 cut(s) 61
BpuEI CTTGAG 2 cut(s) 189, 224
BsaJI CCNNGG 2 cut(s) 6, 414
BseDI CCNNGG 2 cut(s) 6, 414
BseGI GGATG 3 cut(s) 328, 364, 427
BseMII CTCAG 3 cut(s) 129, 284, 378
BseRI GAGGAG 4 cut(s) 33, 36, 54, 64
BseXI GCAGC 3 cut(s) 322, 325, 339
Bsh1236I CGCG 2 cut(s) 8, 222
BshFI GGCC 2 cut(s) 5, 11
BsmAI GTCTC 1 cut(s) 367
BsnI GGCC 2 cut(s) 5, 11
Bsp143I GATC 3 cut(s) 22, 265, 275
Bsp68I TCGCGA 1 cut(s) 222
BspACI CCGC 4 cut(s) 6, 8, 54, 116
BspANI GGCC 2 cut(s) 5, 11
BspCNI CTCAG 3 cut(s) 130, 285, 377
BspFNI CGCG 2 cut(s) 8, 222
BspLI GGNNCC 1 cut(s) 106
BspMAI CTGCAG 1 cut(s) 357
BspPI GGATC 2 cut(s) 17, 273
BssECI CCNNGG 2 cut(s) 6, 414
BssMI GATC 3 cut(s) 22, 265, 275
BssT1I CCWWGG 1 cut(s) 414
Bst6I CTCTTC 2 cut(s) 22, 309
BstAPI GCANNNNNTGC 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 4 cut(s) 138, 293, 338, 364
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 3 cut(s) 328, 364, 427
BstFNI CGCG 2 cut(s) 8, 222
BstKTI GATC 3 cut(s) 25, 268, 278
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 3 cut(s) 22, 265, 275
BstMWI GCNNNNNNNGC 2 cut(s) 175, 434
BstSFI CTRYAG 1 cut(s) 353
BstUI CGCG 2 cut(s) 8, 222
BstV1I GCAGC 3 cut(s) 322, 325, 339
BsuRI GGCC 2 cut(s) 5, 11
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 3 cut(s) 328, 364, 427
BtuMI TCGCGA 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 297
Cfr42I CCGCGG 1 cut(s) 9
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
DdeI CTNAG 4 cut(s) 138, 293, 338, 364
DpnI GATC 3 cut(s) 24, 267, 277
DpnII GATC 3 cut(s) 22, 265, 275
EaeI YGGCCR 2 cut(s) 3, 9
Eam1104I CTCTTC 2 cut(s) 22, 309
EarI CTCTTC 2 cut(s) 22, 309
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FauI CCCGC 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 6, 9, 311, 314, 353
FokI GGATG 3 cut(s) 335, 371, 434
Fsp4HI GCNGC 5 cut(s) 6, 9, 311, 314, 353
FspBI CTAG 2 cut(s) 333, 445
GluI GCNGC 5 cut(s) 6, 9, 311, 314, 353
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 2 cut(s) 5, 11
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 2 cut(s) 31, 128
Hpy188I TCNGA 4 cut(s) 22, 139, 235, 280
Hpy188III TCNNGA 4 cut(s) 221, 241, 287, 366
Hpy99I CGWCG 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 178, 355
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 434
HpyF3I CTNAG 4 cut(s) 138, 293, 338, 364
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 3 cut(s) 22, 265, 275
LmnI GCTCC 2 cut(s) 67, 110
LpnPI CCDG 5 cut(s) 72, 91, 155, 341, 351
Lsp1109I GCAGC 3 cut(s) 322, 325, 339
MaeI CTAG 2 cut(s) 333, 445
MaeIII GTNAC 2 cut(s) 91, 325
MalI GATC 3 cut(s) 24, 267, 277
MboI GATC 3 cut(s) 22, 265, 275
MboII GAAGA 3 cut(s) 9, 296, 407
MluCI AATT 3 cut(s) 119, 153, 281
MmeI TCCRAC 1 cut(s) 164
MslI CAYNNNNRTG 2 cut(s) 206, 260
MspA1I CMGCKG 1 cut(s) 8
MvnI CGCG 2 cut(s) 8, 222
MwoI GCNNNNNNNGC 2 cut(s) 175, 434
NdeII GATC 3 cut(s) 22, 265, 275
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 91
NruI TCGCGA 1 cut(s) 222
OliI CACNNNNGTG 2 cut(s) 206, 260
PfeI GAWTC 2 cut(s) 31, 128
PkrI GCNGC 5 cut(s) 7, 10, 312, 315, 354
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 1 cut(s) 297
PstI CTGCAG 1 cut(s) 357
RruI TCGCGA 1 cut(s) 222
RseI CAYNNNNRTG 2 cut(s) 206, 260
SacII CCGCGG 1 cut(s) 9
SatI GCNGC 5 cut(s) 6, 9, 311, 314, 353
Sau3AI GATC 3 cut(s) 22, 265, 275
Sau96I GGNCC 1 cut(s) 297
SetI ASST 7 cut(s) 114, 137, 144, 191, 231, 420, 446
SfcI CTRYAG 1 cut(s) 353
Sfr303I CCGCGG 1 cut(s) 9
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 2 cut(s) 206, 260
SmlI CTYRAG 2 cut(s) 204, 239
SmoI CTYRAG 2 cut(s) 204, 239
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 3 cut(s) 119, 153, 281
SsiI CCGC 4 cut(s) 6, 8, 54, 116
SspMI CTAG 2 cut(s) 333, 445
StyI CCWWGG 1 cut(s) 414
TaqI TCGA 3 cut(s) 29, 131, 274
TasI AATT 3 cut(s) 119, 153, 281
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 2 cut(s) 31, 128
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 3 cut(s) 310, 313, 352
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 141, 438
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 2 cut(s) 153, 281
XspI CTAG 2 cut(s) 333, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.