MD13G1169200.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002436
Physical Location & Seq
Reverse (-)
13691304 .. 13692305
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1169200.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCTTCTCAACAAGAACCTCTCAAATATCAGACATGGATCTTGAGAGTTCCTATGCACTGTGAAGGTTGCAAGAGGAAGGTCAAAAAAGTTTTACAAAGAACTGATGGTGTTTACACCACAGCTATTGACTCACAGCAAAACAAAGTAACTGTCATTGGAAACGTCGGCATTCAGACCCTCGTCAAGAAGTTGGCCAGGAAAGCAGGCAAGCAAGCACAGGAATGGCCGGAAAAGCTCACCGGAAAAGATAACAAGGCGGAGAAATGCAAGAACAAGGAGAAACAGAAAGTCCCAAACCAACAAAGTGTAGAGAAAGTTGAAGTCCACAAGCTTACCACGGCTCAAAACAGAGCAACTAATACTACTGAAATCGACCCAACTAAGTTTCCCGGCGAAACTTCACAGAATAAGGCCACCGGCGGCGGCGGGTCTCCGGCCGTGGAGGTTCGGATCGGTGAAAGTGAGATGAGTGTGAGTAGCAAAAAGAATAAGGGGAAGGGGCCAAAAGATGATGAGAGTGGTTCAGCAGGCTCTGGTGCACCAGCATGCATAGGATCTCAACCAGTAGATCCAGTTGATCTCTTCCGTACACGCCAGATGGCATATGTGTACCCGCTGGCTCCTTGTCATTCTTATGTTCCATCGTCACCGTACACGTGTGCACCAACTGGTCATCAAGAAGATGACTGGGTGAAGGCAACAGAGCTGGAGAGTTTGCAGGTTTTCAGTGATGAAAACCCTAATGCATGTTTTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

255

Amino Acids

27.8

Weight (kDa)

8.94

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 15 - 68 2e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 712
AciI CCGC 5 cut(s) 260, 423, 426, 429, 617
AclWI GGATC 4 cut(s) 47, 461, 565, 566
AcoI YGGCCR 3 cut(s) 195, 227, 438
AcvI CACGTG 1 cut(s) 660
AfaI GTAC 3 cut(s) 592, 614, 656
AflIII ACRYGT 2 cut(s) 657, 659
AgsI TTSAA 1 cut(s) 323
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 4 cut(s) 125, 238, 334, 709
AluI AGCT 4 cut(s) 125, 238, 334, 709
Alw21I GWGCWC 2 cut(s) 544, 667
Alw26I GTCTC 1 cut(s) 438
Alw44I GTGCAC 2 cut(s) 540, 663
AlwI GGATC 4 cut(s) 47, 461, 565, 566
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 5 cut(s) 195, 227, 414, 438, 503
ApaLI GTGCAC 2 cut(s) 540, 663
ArsI GACNNNNNNTTYG 2 cut(s) 138, 170
AspS9I GGNCC 1 cut(s) 503
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 4 cut(s) 232, 470, 642, 706
BaeGI GKGCMC 2 cut(s) 544, 667
BalI TGGCCA 1 cut(s) 197
BbrPI CACGTG 1 cut(s) 660
Bbv12I GWGCWC 2 cut(s) 544, 667
BccI CCATC 3 cut(s) 101, 595, 652
BceAI ACGGC 2 cut(s) 357, 425
BciT130I CCWGG 1 cut(s) 199
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 712
BisI GCNGC 2 cut(s) 424, 427
BlsI GCNGC 2 cut(s) 425, 428
Bme1390I CCNGG 2 cut(s) 199, 393
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 2 cut(s) 504, 624
BmrFI CCNGG 2 cut(s) 199, 393
BmrI ACTGGG 1 cut(s) 700
BmuI ACTGGG 1 cut(s) 700
BoxI GACNNNNGTC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 731
BpuEI CTTGAG 1 cut(s) 64
BpuMI CCSGG 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 660
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 339, 441
BsaWI WCCGGW 1 cut(s) 242
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 4 cut(s) 566, 575, 676, 695
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 2 cut(s) 339, 441
BseNI ACTGG 4 cut(s) 566, 575, 676, 695
BseSI GKGCMC 2 cut(s) 544, 667
BseX3I CGGCCG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 5 cut(s) 197, 229, 416, 440, 505
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 2 cut(s) 544, 667
BsiSI CCGG 5 cut(s) 230, 243, 393, 420, 437
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 5 cut(s) 197, 229, 416, 440, 505
Bso31I GGTCTC 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 544, 667
Bsp143I GATC 5 cut(s) 39, 453, 557, 571, 580
BspACI CCGC 5 cut(s) 260, 423, 426, 429, 617
BspANI GGCC 5 cut(s) 197, 229, 416, 440, 505
BspLI GGNNCC 2 cut(s) 504, 624
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 4 cut(s) 47, 461, 565, 566
BspTNI GGTCTC 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 4 cut(s) 566, 575, 676, 695
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 339, 441
BssMI GATC 5 cut(s) 39, 453, 557, 571, 580
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 3 cut(s) 62, 154, 654
Bst6I CTCTTC 1 cut(s) 590
BstBAI YACGTR 1 cut(s) 660
BstC8I GCNNGC 6 cut(s) 208, 212, 216, 532, 550, 621
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 2 cut(s) 339, 441
BstKTI GATC 5 cut(s) 42, 456, 560, 574, 583
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 5 cut(s) 39, 453, 557, 571, 580
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 2 cut(s) 203, 235
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 552, 753
BstPAI GACNNNNGTC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 197, 391
BstSLI GKGCMC 2 cut(s) 544, 667
BstX2I RGATCY 3 cut(s) 39, 557, 571
BstYI RGATCY 3 cut(s) 39, 557, 571
BstZI CGGCCG 1 cut(s) 438
BsuRI GGCC 5 cut(s) 197, 229, 416, 440, 505
BtgI CCRYGG 2 cut(s) 339, 441
BtsIMutI CAGTG 2 cut(s) 58, 736
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 6 cut(s) 208, 212, 216, 532, 550, 621
CaiI CAGNNNCTG 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 503
Csp6I GTAC 3 cut(s) 591, 613, 655
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviAII CATG 4 cut(s) 36, 549, 750, 760
CviQI GTAC 3 cut(s) 591, 613, 655
DdeI CTNAG 1 cut(s) 384
DpnI GATC 5 cut(s) 41, 455, 559, 573, 582
DpnII GATC 5 cut(s) 39, 453, 557, 571, 580
EaeI YGGCCR 3 cut(s) 195, 227, 438
EagI CGGCCG 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
EciI GGCGGA 1 cut(s) 275
EclXI CGGCCG 1 cut(s) 438
Eco31I GGTCTC 1 cut(s) 438
Eco52I CGGCCG 1 cut(s) 438
Eco72I CACGTG 1 cut(s) 660
EcoRII CCWGG 1 cut(s) 197
EcoT22I ATGCAT 2 cut(s) 554, 751
FaeI CATG 4 cut(s) 39, 552, 753, 763
FaiI YATR 9 cut(s) 37, 56, 550, 554, 607, 609, 639, 751, 761
FaqI GGGAC 1 cut(s) 278
FatI CATG 4 cut(s) 35, 548, 749, 759
FauI CCCGC 2 cut(s) 422, 624
FauNDI CATATG 1 cut(s) 607
Fnu4HI GCNGC 2 cut(s) 424, 427
Fsp4HI GCNGC 2 cut(s) 424, 427
GluI GCNGC 2 cut(s) 424, 427
GsuI CTGGAG 1 cut(s) 731
HaeIII GGCC 5 cut(s) 197, 229, 416, 440, 505
HapII CCGG 5 cut(s) 230, 243, 393, 420, 437
Hin1II CATG 4 cut(s) 39, 552, 753, 763
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 1 cut(s) 131
HpaII CCGG 5 cut(s) 230, 243, 393, 420, 437
HphI GGTGA 4 cut(s) 232, 470, 642, 706
Hpy166II GTNNAC 7 cut(s) 115, 328, 542, 593, 613, 657, 665
Hpy188I TCNGA 3 cut(s) 33, 177, 453
Hpy188III TCNNGA 3 cut(s) 43, 187, 680
Hpy8I GTNNAC 7 cut(s) 115, 328, 542, 593, 613, 657, 665
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 4 cut(s) 59, 73, 493, 691
HpyCH4III ACNGT 3 cut(s) 62, 154, 654
HpyCH4IV ACGT 2 cut(s) 165, 659
HpyCH4V TGCA 8 cut(s) 58, 72, 270, 542, 552, 665, 721, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 235
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 2 cut(s) 165, 659
Hsp92II CATG 4 cut(s) 39, 552, 753, 763
Kzo9I GATC 5 cut(s) 39, 453, 557, 571, 580
LmnI GCTCC 1 cut(s) 628
MaeII ACGT 2 cut(s) 165, 659
MaeIII GTNAC 2 cut(s) 148, 648
MalI GATC 5 cut(s) 41, 455, 559, 573, 582
MboI GATC 5 cut(s) 39, 453, 557, 571, 580
MboII GAAGA 2 cut(s) 577, 695
MflI RGATCY 3 cut(s) 39, 557, 571
MhlI GDGCHC 2 cut(s) 544, 667
MlsI TGGCCA 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 4 cut(s) 30, 69, 191, 439
Mox20I TGGCCA 1 cut(s) 197
Mph1103I ATGCAT 2 cut(s) 554, 751
MscI TGGCCA 1 cut(s) 197
MslI CAYNNNNRTG 3 cut(s) 223, 547, 636
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 619
MspI CCGG 5 cut(s) 230, 243, 393, 420, 437
MspR9I CCNGG 2 cut(s) 199, 393
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 203, 235
NciI CCSGG 1 cut(s) 393
NdeI CATATG 1 cut(s) 607
NdeII GATC 5 cut(s) 39, 453, 557, 571, 580
NlaIII CATG 4 cut(s) 39, 552, 753, 763
NlaIV GGNNCC 2 cut(s) 504, 624
NmuCI GTSAC 1 cut(s) 648
NsiI ATGCAT 2 cut(s) 554, 751
NspI RCATGY 2 cut(s) 552, 753
PaeI GCATGC 1 cut(s) 552
PctI GAATGC 1 cut(s) 171
PkrI GCNGC 2 cut(s) 425, 428
PleI GAGTC 1 cut(s) 125
PmaCI CACGTG 1 cut(s) 660
PmlI CACGTG 1 cut(s) 660
PpsI GAGTC 1 cut(s) 125
Ppu21I YACGTR 1 cut(s) 660
PshAI GACNNNNGTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 197
PspCI CACGTG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 504, 624
PspPI GGNCC 1 cut(s) 503
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 3 cut(s) 39, 557, 571
RsaI GTAC 3 cut(s) 592, 614, 656
RsaNI GTAC 3 cut(s) 591, 613, 655
RseI CAYNNNNRTG 3 cut(s) 223, 547, 636
SatI GCNGC 2 cut(s) 424, 427
Sau3AI GATC 5 cut(s) 39, 453, 557, 571, 580
Sau96I GGNCC 1 cut(s) 503
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 199, 393
SduI GDGCHC 2 cut(s) 544, 667
SgrAI CRCCGGYG 1 cut(s) 419
SmiMI CAYNNNNRTG 3 cut(s) 223, 547, 636
SmlI CTYRAG 1 cut(s) 43
SmoI CTYRAG 1 cut(s) 43
SphI GCATGC 1 cut(s) 552
SsiI CCGC 5 cut(s) 260, 423, 426, 429, 617
StyD4I CCNGG 2 cut(s) 197, 391
TaaI ACNGT 3 cut(s) 62, 154, 654
TaiI ACGT 2 cut(s) 168, 662
TaqI TCGA 1 cut(s) 375
TauI GCSGC 2 cut(s) 426, 429
TscAI CASTG 2 cut(s) 65, 736
TseFI GTSAC 1 cut(s) 648
Tsp45I GTSAC 1 cut(s) 648
TspDTI ATGAA 1 cut(s) 750
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 2 cut(s) 65, 736
VneI GTGCAC 2 cut(s) 540, 663
XceI RCATGY 2 cut(s) 552, 753
Zsp2I ATGCAT 2 cut(s) 554, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.