Rw4G020490

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002436
Physical Location & Seq
Forward (+)
44506491 .. 44507566
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020490.1

Sequence Viewer

Length: 858 bp
ATGGCTTCTGTATTGTCTGCTCAAGAAGCCTCACAGCTACTCAAATGTCAGACATGGATCTTGAAAGTGTTTATCCACTGTGAAGGTTGCAAGAGGAAAGTGAAGAAAGTTCTACTGGGAATTGATGGTGTTTTCACCACATCTATTGAGGCAGAGCAAAACAGAGTCACAGTCACTGGAGACATTGACGTTCAGACCCTCATCAAGAAGCTGAATGTAAAAACTGGTAAACATGCCGAGCTATGGCCAGAAAATCTCACCAAGAAAGAAAAGACCAAGCCTGTGAAAGCCAAGAACAACGACAAACAGAAATCCTCAGAAATTTGCCAAAGTAAGCAGAGTACTACATGTGACAAAGTTGGAATGGTCAGGCTAAACAGAGCAAGTGAGGGTGACACAGCTAAGTTTAACAGAGAGGAACAATGTCAAGTATCAAAGAAGGGTCACATTCCGCCGGAGAAATCTCCTGATGGCCATGATTCTCAGGCCATGGATCTTCAGCTCGGTGAAAGTGAAAAAGGAAGTGTTAGTGGCAAAAAGAACAGGAAAGGCCAAAGAGATGATCTGGGATCTCAACATTATGGTGATCACACTGCTGGTCCAGTTGATCTCAGCCATATCATCGCAAACATGTATCCGATGGATTACCACTCTCATCTGGTGTTCAATGGAAGTGACATTACAGCAGCTGCTACAGCACTGCCTAGCAAGAGCTTGGCTCCCTACTATTGTTATGGTCCATCATCACAGTACAAGGATGCAGCTAGTGGTGAACAGATTGATCAAGTGAAAGCAACATTTCTTAACAGTGTGCAGATTTTCAGTGATGAAAATGCTAATGGCTGTTTTATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

285

Amino Acids

31.34

Weight (kDa)

8.5

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 22 - 71 2.6e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 452
AclWI GGATC 3 cut(s) 65, 501, 577
AcoI YGGCCR 2 cut(s) 245, 472
AcsI RAATTY 1 cut(s) 321
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 2 cut(s) 343, 752
AfiI CCNNNNNNNGG 1 cut(s) 243
AflIII ACRYGT 2 cut(s) 347, 630
AgsI TTSAA 2 cut(s) 64, 667
AluBI AGCT 8 cut(s) 37, 211, 241, 401, 502, 689, 714, 764
AluI AGCT 8 cut(s) 37, 211, 241, 401, 502, 689, 714, 764
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 65, 501, 577
AlwNI CAGNNNCTG 2 cut(s) 176, 689
AoxI GGCC 4 cut(s) 245, 472, 486, 550
ApeKI GCWGC 3 cut(s) 686, 689, 761
ApoI RAATTY 1 cut(s) 321
AspS9I GGNCC 2 cut(s) 599, 737
AsuHPI GGTGA 6 cut(s) 127, 250, 404, 518, 596, 782
AvaII GGWCC 2 cut(s) 599, 737
BalI TGGCCA 2 cut(s) 247, 474
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbvI GCAGC 3 cut(s) 676, 698, 773
BccI CCATC 4 cut(s) 119, 464, 634, 748
BciVI GTATCC 1 cut(s) 645
BclI TGATCA 2 cut(s) 586, 781
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 705, 765
BfmI CTRYAG 1 cut(s) 693
BfuI GTATCC 1 cut(s) 645
BisI GCNGC 3 cut(s) 687, 690, 762
BlsI GCNGC 3 cut(s) 688, 691, 763
BmcAI AGTACT 1 cut(s) 343
Bme18I GGWCC 2 cut(s) 599, 737
BmgT120I GGNCC 2 cut(s) 599, 737
BmiI GGNNCC 1 cut(s) 720
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 748
BmuI ACTGGG 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 703, 735
BpmI CTGGAG 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 489
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 4 cut(s) 120, 181, 229, 602
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 763
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 3 cut(s) 330, 497, 625
BseNI ACTGG 4 cut(s) 120, 181, 229, 602
BseXI GCAGC 3 cut(s) 676, 698, 773
BsgI GTGCAG 1 cut(s) 833
BshFI GGCC 4 cut(s) 247, 474, 488, 552
BsiSI CCGG 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 1 cut(s) 174
BsnI GGCC 4 cut(s) 247, 474, 488, 552
Bsp143I GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 1 cut(s) 452
BspANI GGCC 4 cut(s) 247, 474, 488, 552
BspCNI CTCAG 3 cut(s) 329, 496, 624
BspLI GGNNCC 1 cut(s) 720
BspPI GGATC 3 cut(s) 65, 501, 577
BsrI ACTGG 4 cut(s) 120, 181, 229, 602
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 4 cut(s) 80, 172, 750, 809
BstDEI CTNAG 4 cut(s) 316, 402, 483, 611
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 1 cut(s) 763
BstKTI GATC 7 cut(s) 60, 496, 565, 572, 589, 610, 784
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
BstMWI GCNNNNNNNGC 2 cut(s) 26, 695
BstNSI RCATGY 3 cut(s) 236, 351, 634
BstSFI CTRYAG 1 cut(s) 693
BstV1I GCAGC 3 cut(s) 676, 698, 773
BstX2I RGATCY 3 cut(s) 57, 493, 569
BstYI RGATCY 3 cut(s) 57, 493, 569
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 4 cut(s) 247, 474, 488, 552
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 1 cut(s) 763
BtsI GCAGTG 2 cut(s) 591, 698
BtsIMutI CAGTG 6 cut(s) 76, 174, 591, 698, 814, 829
CaiI CAGNNNCTG 2 cut(s) 176, 689
Cfr13I GGNCC 2 cut(s) 599, 737
Csp6I GTAC 2 cut(s) 342, 751
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviAII CATG 6 cut(s) 54, 233, 348, 476, 490, 631
CviQI GTAC 2 cut(s) 342, 751
DdeI CTNAG 4 cut(s) 316, 402, 483, 611
DpnI GATC 7 cut(s) 59, 495, 564, 571, 588, 609, 783
DpnII GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
EaeI YGGCCR 2 cut(s) 245, 472
EciI GGCGGA 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 489
Eco47I GGWCC 2 cut(s) 599, 737
Eco57I CTGAAG 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 6 cut(s) 57, 236, 351, 479, 493, 634
FatI CATG 6 cut(s) 53, 232, 347, 475, 489, 630
FbaI TGATCA 2 cut(s) 586, 781
Fnu4HI GCNGC 3 cut(s) 687, 690, 762
FokI GGATG 1 cut(s) 770
Fsp4HI GCNGC 3 cut(s) 687, 690, 762
FspBI CTAG 2 cut(s) 705, 765
GluI GCNGC 3 cut(s) 687, 690, 762
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 4 cut(s) 247, 474, 488, 552
HapII CCGG 1 cut(s) 455
Hin1II CATG 6 cut(s) 57, 236, 351, 479, 493, 634
HinfI GANTC 2 cut(s) 165, 479
HpaII CCGG 1 cut(s) 455
HphI GGTGA 6 cut(s) 127, 250, 404, 518, 596, 782
Hpy166II GTNNAC 2 cut(s) 230, 773
Hpy188I TCNGA 4 cut(s) 51, 195, 319, 639
Hpy188III TCNNGA 4 cut(s) 23, 61, 205, 467
Hpy8I GTNNAC 2 cut(s) 230, 773
HpyAV CCTTC 2 cut(s) 77, 433
HpyCH4III ACNGT 4 cut(s) 80, 172, 750, 809
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 3 cut(s) 90, 761, 814
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 695
HpyF3I CTNAG 4 cut(s) 316, 402, 483, 611
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 6 cut(s) 57, 236, 351, 479, 493, 634
Ksp22I TGATCA 2 cut(s) 586, 781
Kzo9I GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
LmnI GCTCC 1 cut(s) 724
Lsp1109I GCAGC 3 cut(s) 676, 698, 773
LweI GCATC 1 cut(s) 748
MaeI CTAG 2 cut(s) 705, 765
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 6 cut(s) 166, 172, 350, 392, 443, 674
MalI GATC 7 cut(s) 59, 495, 564, 571, 588, 609, 783
MboI GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
MboII GAAGA 2 cut(s) 115, 488
MflI RGATCY 3 cut(s) 57, 493, 569
MlsI TGGCCA 2 cut(s) 247, 474
MluCI AATT 2 cut(s) 120, 321
MluNI TGGCCA 2 cut(s) 247, 474
MlyI GAGTC 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 7 cut(s) 40, 87, 142, 209, 325, 382, 409
Mox20I TGGCCA 2 cut(s) 247, 474
MscI TGGCCA 2 cut(s) 247, 474
MseI TTAA 2 cut(s) 408, 804
MslI CAYNNNNRTG 1 cut(s) 582
Msp20I TGGCCA 2 cut(s) 247, 474
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 1 cut(s) 455
MwoI GCNNNNNNNGC 2 cut(s) 26, 695
NcoI CCATGG 1 cut(s) 489
NdeII GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
NlaIII CATG 6 cut(s) 57, 236, 351, 479, 493, 634
NlaIV GGNNCC 1 cut(s) 720
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 6 cut(s) 166, 172, 350, 392, 443, 674
NspI RCATGY 3 cut(s) 236, 351, 634
PciI ACATGT 2 cut(s) 347, 630
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 3 cut(s) 688, 691, 763
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PscI ACATGT 2 cut(s) 347, 630
PspN4I GGNNCC 1 cut(s) 720
PspPI GGNCC 2 cut(s) 599, 737
PstNI CAGNNNCTG 2 cut(s) 176, 689
PsuI RGATCY 3 cut(s) 57, 493, 569
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 2 cut(s) 343, 752
RsaNI GTAC 2 cut(s) 342, 751
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 2 cut(s) 408, 804
SatI GCNGC 3 cut(s) 687, 690, 762
Sau3AI GATC 7 cut(s) 57, 493, 562, 569, 586, 607, 781
Sau96I GGNCC 2 cut(s) 599, 737
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 1 cut(s) 174
SfaNI GCATC 1 cut(s) 748
SfcI CTRYAG 1 cut(s) 693
SinI GGWCC 2 cut(s) 599, 737
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 2 cut(s) 120, 321
SsiI CCGC 1 cut(s) 452
SspMI CTAG 2 cut(s) 705, 765
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 80, 172, 750, 809
TaiI ACGT 1 cut(s) 192
TasI AATT 2 cut(s) 120, 321
TatI WGTACW 2 cut(s) 341, 750
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 2 cut(s) 408, 804
Tru9I TTAA 2 cut(s) 408, 804
TscAI CASTG 6 cut(s) 83, 181, 598, 705, 814, 829
TseFI GTSAC 6 cut(s) 166, 172, 350, 392, 443, 674
TseI GCWGC 3 cut(s) 686, 689, 761
Tsp45I GTSAC 6 cut(s) 166, 172, 350, 392, 443, 674
TspDTI ATGAA 1 cut(s) 843
TspRI CASTG 6 cut(s) 83, 181, 598, 705, 814, 829
VpaK11BI GGWCC 2 cut(s) 599, 737
XapI RAATTY 1 cut(s) 321
XceI RCATGY 3 cut(s) 236, 351, 634
XspI CTAG 2 cut(s) 705, 765
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.